Effects of frequency of irrigation and gypsum treatment on leaf water potential and leaf stomatal conductance of lucerne (Medicago sativa L.) grown on a heavy clay soil

Summary Concurrent diurnal measurements of water potential, osmotic potential and conductance were made on leaves of lucerne grown under weekly (W) and fortnightly (F) irrigation on gypsum-treated (G) and untreated soil (C). Measurements were made throughout the period of vegetative growth.Leaf water potentials were lower both at dawn and in the afternoon under fortnightly as compared to weekly irrigation. Gypsum application led to a slower decline in water potential under fortnightly irrigation, although the effect was small compared with more frequent irrigation. Stomatal conductance was reduced under treatments F_G and F_C during the later stages of vegetative growth, coinciding with leaf water potentials of less than c. –1.6 MPa.The relationship between leaf water potential and turgor potential changed with time such that positive turgor was maintained as leaf water potential declined. Turgor maintenance was achieved through a decrease in leaf osmotic potential. These data suggest that lucerne is capable of osmotic adjustment.Stomatal conductance declined rapidly below a leaf turgor potential of c. 0.1 MPa. It is hypothesised that osmotic adjustment enabled stomatal adjustment, which contributed to continued assimilation despite increasing soil moisture deficits.     

The effects of partial root-zone irrigation and regulated deficit irrigation on the vegetative and reproductive development of field-grown Monastrell grapevines

The effects of regulated deficit irrigation (RDI) and partial root-zone irrigation (PRI) strategies that apply the same irrigation volumes on vegetative and reproductive development were analyzed during a 3-year-period in field-grown Monastrell grapevines under semiarid conditions. Five treatments were applied: control irrigated at 60?% ETc (crop evapotranspiration) for the whole season (308?mm?year^?1); RDI-1 and PRI-1 that received the same irrigation as the control before fruit set, 30?% ETc from fruit set to harvest and 45?% ETc post-harvest (192?mm?year^?1); and RDI-2 and PRI-2 that were the same, except with 15?% ETc from fruit set to harvest (142?mm?year^?1). Distinctive PRI effects on vegetative and reproductive development were observed depending on the total soil water content and the vine water stress level. PRI-1 vines showed less restriction of vegetative growth, lower leaf abscission, and higher leaf area during post-veraison than RDI-1 vines. Higher supply of water (close to field capacity) via half of the root system in PRI-1 vines maintained better water supply and more favorable phloem sap flow (water and carbohydrates) into the fruit during post-veraison and showed a positive differential effect on fruit growth compared with RDI-1 vines. This was reflected in a higher solute content per berry (12?% higher) and higher fresh berry weight (8?% higher) at harvest in PRI-1 compared to RDI-1 berries. However, this positive effect in fruit growth was not reflected in either an improved final yield or the water use efficiency of PRI-1 vines. In PRI-2, the soil water in the wet half was insufficient to maintain more favorable shoot water supply and phloem sap flow into the berry, and no substantial changes were observed in vine vigor, leaf and fruit growth between PRI-2 and RDI-2. Higher irrigation amount in the wet root zone and higher depth of irrigation under PRI seem to be more effective for 1103P–Mourvedre combination to produce a favorable effect in berry growth and development.     

Is partial root-zone drying an effective irrigation technique to improve water use efficiency and fruit quality in field-grown wine grapes under semiarid conditions?

We investigated the effects of partial root-zone drying (PRD) applied at different periods on leaf water relations, vegetative development, fruit yield, must and wine quality in wine grapes (Vitis vinifera L. cv. Monastrell) during a 3-year field experiment in order to determine the importance of the timing of PRD application on physiological and agronomical vine response under semiarid conditions. Two irrigation treatments were applied: conventional drip irrigation (CI) and PRD. Both treatments received the same annual water quantity. Each year the PRD treatment was applied at different periods of the growth cycle. In 1999 PRD was applied from veraison to harvest (end July–early September); in 2000 from fruit set to harvest (mid June–early September); and in 2001 PRD from budburst to harvest (mid April–early September). Leaf water relations and gas exchange during the experimental period were not significantly affected by PRD treatment. In 1999 and 2000 there was no significant treatment effect on vegetative development, yield or fruit quality. However, in 2001 (when PRD was applied from budburst to harvest), reproductive and vegetative development was clearly altered in PRD vines. Fruit set percentage and vegetative development (shoot length, pruning weight and primary and lateral leaf area) were significantly increased in PRD vines compared to CI. This resulted in both higher yield (kg per vine) (43%) and water use efficiency (40%) compared to CI vines. Berry number per cluster and cluster weight were also significantly increased in PRD vines. Notwithstanding higher yield in PRD vines and a similar berry size, the must and wine quality was not significantly altered, indicating a higher synthesis and accumulation of photoassimilates and metabolites in the berries of PRD vines. We conclude that there was an positive effect on vegetative and reproductive growth when long-term PRD was applied from the beginning of growing season (budburst), suggesting that early onset of PRD is desirable to intensify PRD response under these semiarid conditions. Nevertheless from these results we need to further investigate the long- and short-term effects of PRD, with moderate water amounts, on vegetative and reproductive development such as flowering and fruit set processes in wine grapes.     

Response of tomatoes,a crop of indeterminate growth,to soil salinity

Tomatoes were grown in tanks filled with loam and clay, and were irrigated with waters of three different levels of salinity. Osmotic adjustment was determined by analysing the pressure–volume curves at four growth stages. Owing to the osmotic adjustment, tomatoes are able to maintain the turgor potential and the stomatal conductance at the same value for the lower values of the leaf-water potential. Salinity affected the pre-dawn leaf-water potential, stomatal conductance, evapotranspiration, leaf area and fruit yield on both soils. Soil texture only affected the fruit yield. The evapotranspiration showed a moderate decrease, owing to the small decrease in leaf area and the effect of osmotic adjustment on the stomatal conductance, whereas the fruit yield decreased strongly. The tomato plant apparently favours under saline conditions, the growth of foliage at the expense of fruit formation.     

Osmotic adjustment of sugar beets in response to soil salinity and its influence on stomatal conductance,growth and yield

Sugar beets were grown in tanks filled with loam and clay, and were irrigated with waters of three different levels of salinity. Osmotic adjustment was determined by analyzing the pressure-volume curves at three growth stages. Sugar beets showed osmotic adjustment in two ways: with their phenological development and towards salinity. Owing to the latter adjustment sugar beets are able to maintain the turgor potential at the same value for lower values of the leaf water potential, to maintain stomatal conductance and photosynthesis and finally their production under severe water stress.Salinity affected the pre-dawn leaf water potential, stomatal conductance and evapotranspiration on both soils, but leaf area and yield only on loam.Soil texture affected stomatal conductance, evapotranspiration, leaf area and yield. As the latter was about 35% lower on clay, whereas the evapotranspiration decreased 10 to 15%, the water use efficiency was about 25% lower on clay compared with loam.     

Whole-tree water balance and indicators for short-term drought stress in non-bearing ‘Barnea’ olives

Drainage-weighing lysimeters allowed monitoring of water balance components of non-bearing olive (Olea europaea cv Barnea) trees over a 3-month period including short-term events of controlled but severe water stress. The objective of the study was to evaluate a variety of soil and plant-based water status and drought stress monitoring methods on the basis of tree-scale evapotranspiration (ET). As the trees entered into and recovered from water stress, meteorological data, actual ET (ET_a), soil water content and changes in leaf turgor pressure were continuously monitored. Additionally, midday measurements of stem water potential, stomatal conductance, canopy temperature, and quantum yield of PSII photochemistry were conducted. Diurnal (dawn to dusk) measurements of all the above were made hourly on days of maximum stress. Shoot elongation rate was measured for periods of stress and recovery. Quantum yield of PSII photochemistry, stomatal conductance, and stem water potential all successfully indicated reductions in whole-tree water consumption beginning at moderate stress levels. These measured parameters fully recovered to the levels of non-stressed trees soon after water application was renewed. Shoot elongation was reduced 25-30% for the 10-day period during and following drought and recovered thereafter to levels of non-stressed trees. Whole-tree ET_a was reduced by as much as 20% even following full recovery of the leaf level parameters, suggesting reduced canopy size and growth due to the stress period. Non-destructive, continuous (turgor pressure) and remotely sensed (canopy temperature) methods showed promising potential for monitoring effects of water stress, in spite of technological and data interpretation challenges requiring further attention.     

Isohydrodynamic behavior in deficit-irrigated Cabernet Sauvignon and Malbec and its relationship between yield and berry composition

Cabernet Sauvignon and Malbec grapevines were irrigated at 70 or 23 % of estimated crop evapotranspiration throughout berry development over four growing seasons. Stomatal behavior was characterized by relating predawn leaf water potential and mid-morning stomatal conductance to mid-morning leaf water potential. Seasonal average weekly midday leaf water potential was lower in Cabernet Sauvignon than Malbec despite similar irrigation amounts. Both cultivars exhibited anisohydric behavior with midday leaf water potential decreasing linearly with declining predawn leaf water potential (r ² = 0.51) and stomatal conductance (r ² = 0.42). However, both cultivars utilized hydrodynamic mechanisms to maintain a soil-to-leaf water potential gradient of ?0.62 (±0.03) MPa under standard irrigation and ?0.75 (±0.04) MPa under reduced irrigation. Berry fresh weight and titratable acidity decreased, and the concentration of total anthocyanins increased in both cultivars in response to decreases in midday leaf water potential. The slope of regression equations for seasonal mean midday leaf water potential was used to estimate cultivar-specific levels of water stress associated with changes in berry weight and berry composition at fruit maturity.     

Water relations of field grown Pomegranate trees (Punica granatum) under different drip irrigation regimes

Pomegranate (Punica granatum L.) is a deciduous fruit tree native of central Asia included in the so-called group of minor fruit tree species, not widely grown but of some importance in the south east of Spain. Fruit consumption interest is due to the organoleptic characteristics and to the beneficial effects on health. Pomegranate tree are considered as a culture tolerant to soil water deficit. However, very little is known about pomegranate orchard water management. The objective of this work was to characterize, for the first time in P. granatum, water relations aspects of applied significance for irrigation scheduling. Trees under different irrigation regimes were used and midday stem water potential (Ψ_stem) and midday leaf gas exchange were periodically measured over the course of an entire season. During spring and autumn, Ψ_stem did not show significant differences between irrigation treatments while there were considerable differences in leaf photosynthesis and stomatal conductance, suggesting a near-isohydric behaviour of pomegranate trees. This might explain why the signal intensity of Ψ_stem was lower than those of gas exchange indicators during the experimental period. Thus, leaf photosynthesis rates and stomatal conductance might have a greater potential for irrigation scheduling of pomegranate trees than Ψ_stem measured at solar noon.     

水分胁迫对枇杷果实发育阶段的光合特性和果实品质的影响

以6年生大五星枇杷为试材,系统研究了果实发育阶段水分胁迫处理对枇杷叶片的光合特性和果实品质的影响,结果表明:随水分胁迫加剧,叶片的净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Cs)下降,水分利用率(WUE)提高。不同水分处理的Pn日变化曲线呈双峰型,具有明显的"午休"现象。果实的可溶性固形物、总糖、总酸的含量随水分胁迫加剧而提高。水分胁迫导致果实单果重和果实含水率显著下降。该研究为攀西干旱河谷枇杷果实发育阶段的水分管理提供决策支持。     

Combined effects of water stress and fruit thinning on fruit and vegetative growth of a very early-maturing peach cultivar: assessment by means of a fruit tree model, QualiTree

Regulated deficit irrigation strategies are common practices in areas with low water availability. Thus, water stress, which can limit fruit growth, is imposed to the trees. Fruit thinning can be used to relieve this water stress in peach. In this paper, the ability of an existing fruit tree model (QualiTree) for describing the effects of water stress and fruit thinning on peach fruit and vegetative growth was assessed. The model was parameterized and calibrated for a very early-maturing peach cultivar (“Flordastar”). Important parameters were those expressing the effect of distance between organs on carbon exchange within the tree, the potential dry masses, and the relative growth rates of fruits and leafy shoots. Then, the model was tested in a wide range of water stress situations and three fruit thinning intensities: no thinning, commercial thinning, and heavy thinning. Fruit and vegetative growth simulations were consistent with observed data derived from 2006 field experiments. The variability over time of fruit and vegetative growth was well predicted. The model reproduced reductions in fruit growth observed in field experiments. It also reacted to simulated scenarios that combined water stress and thinning. Increasing thinning intensity reduced total fruit yield but increased fruit size at harvest, compensating the negative effects of water stress on fruit growth. These simulations broadened the predictive capabilities of the model and showed that it might be a useful tool in the design of innovative horticultural practices.     

Responses of “Chardonnay” to deficit irrigation applied at different phenological stages: vine growth, must composition, and wine quality

Adoption of water-saving irrigation strategies is necessary especially for grapevine that has the highest acreage of any fruit crop in the world. We applied deficit irrigation to Chardonnay wine grape at the following phenological stages: anthesis to fruit set, fruit set to veraison, and veraison to harvest. Four irrigation levels (0, 25, 50, and 100?% of crop evapotranspiration, ET _c ) were applied in 2009. Vines grown in large containers were used to enable imposition of water stress early in the growing season. The following parameters were measured: midday leaf water potential, vine growth, yield, and quality of must and wine. The same parameters were measured in 2010 although all vines were fully irrigated. The 0 and 25?% treatments caused defoliation and had negative impacts on yield and wine quality in both 2009 and 2010. Chardonnay was most sensitive to water stress in post-veraison in terms of productivity and wine quality.     

Simulation of possible adaptive mechanisms in crops subjected to water stress

Summary The dynamics of stress development in crops involves a decrease in turgor potential of leaves which causes decreases in leaf expansion, photosynthesis, and transpiration. A study was conducted to evaluate the effectiveness of three possible adaptive mechanisms in maintaining turgor potential and growth. These mechanisms — osmotic adjustment, increased root growth, and increased stomatal resistance at full turgor — were examined by a simulation experiment using a dynamic model of the soil-crop-atmospheric system. Osmotic adjustment was found to be ineffective in maintaining turgor for crops grown in a sandy soil because of the rapid development of stress. When a ten-day drying cycle was simulated for a clay soil, cumulative transpiration, photosynthesis and growth were increased by osmotic adjustment, indicating an improved ability of the crop to maintain turgor under the simulated conditions for the clay soil. increased stomatal resistance was ineffective for the simulated conditions because of a concomitant decrease in photosynthetic rate. Increased soil volume occupied by roots was found to be the most effective adaptive mechanism for maintaining turgor, transpiration, photosynthesis and growth of crops in both soil types.Contribution from Department of Agricultural Engineering, Institute of Food and Agricultural Sciences, University of Florida. Supported in part by Grant No. FL-AGO-1911 Cooperative Research, United States Department of Agriculture. Florida Agricultural Experiment Station Journal No. 2657     

Water potential and turgor pressure as a selection basis for wind-grown winter wheat

Two varieties of winter wheat (Triticum aestivum L. em. Thell. ‘Ponca’ and ‘KanKing’), one considered drought sensitive (‘Ponca’) and one considered drought resistant (‘KanKing’), were grown from seed in pots of soil placed in wind or still air for 36 days to determine the effect of wind on water potentials, turgor pressure, and stomatal resistance of leaves. During the experiment, plants were well watered and soil was moist. In wind and still air, water potentials and turgor pressures of the drought sensitive wheat were higher, and stomatal resistances were lower, than those of the drought resistant wheat. Plants grown in wind had lower turgor pressures than plants grown in still air.The results showed that wind reduced turgor pressure and that measurements of water potential, turgor pressure, and stomatal resistance could be used to select varieties for windy areas.     

Response of sorghum to moisture stress using line source sprinkler irrigation I. Plant-water relations

The response of sorghum (Sorghum bicolor L.) to moisture stress during the post-rainy season was studied at ICRISAT research center on a medium deep Alfisol using a line source sprinkler irrigation system. Changes in soil moisture content, stomatal conductance, leaf-water potential and leaf temperature of sorghum as a function of distance away from the line source sprinkler system were monitored throughout the season. Use of the line source technique facilitated the imposition of a range of moisture stress levels as indicated by increased water use by sorghum closer to the line source compared with the crop farther away from the line source. Canopy response measured in terms of stomatal conductance, leaf-water potential, and leaf temperature clearly reflected the gradient in moisture stress perpendicular to the line source.     

A partial root zone drying irrigation strategy for citrus—Effects on water use efficiency and fruit characteristics

In Australian irrigated citriculture, fruit yield and quality outcomes are not tightly related to levels of plant available water, which raises the possibility of using mild water stress applied to part of the root zone, i.e. partial root zone drying, to stimulate physiological mechanisms that reduce tree water use by changing the relationship between stomatal conductance and ambient evaporative conditions.The PRD technique alternates irrigation such that one side of the tree root zone is allowed to dry whilst the other side is irrigated. This significantly reduces the wetted soil volume at any point in time, whilst always maintaining a readily available water supply to part of the root zone. By adopting this irrigation strategy water use of mature Navel orange trees C. sinensis (L.) Osbeck was reduced and water use efficiency was increased. The technique did not induce excessive fruitlet drop and crop yield was unaffected. Both fruit size and juice percentage slightly decreased whereas total soluble solids percentage (TSS) and juice acid percentage increased. As water use was reduced and juice quality attributes were increased, this technique has obvious benefits for juice fruit production.PRD offers an advantage over conventional deficit irrigation strategies because it helps reduce water use by separating the biochemical signaling responses to water deficit in the dry part of the root zone from the physical effects of reduced stomatal conductance due to lower water availability, allowing developmental processes associated with plant growth to remain unperturbed. Irrigating a reduced root zone volume in this way reduces crop water requirement. However, it is also important to understand that this technique pushes the crop to its limits and should only be applied to well established healthy trees.When applying PRD irrigation, it is important that water is supplied with sufficient frequency and depth of wetting to meet the water needs of the whole plant.     

Optimal levels of postharvest deficit irrigation for promoting early flowering and harvest dates in loquat (Eriobotrya japonica Lindl.)

Deficit irrigation after harvest has been proven to be a more profitable strategy for producing loquats due to its effects on promoting earlier flowering and harvest date next season. To determine water savings which most advance flowering and harvest dates, an experiment was established to compare phenology, fruit quality and yield in ‘Algerie’ loquats over two consecutive seasons. In this experiment some trees were programmed to receive 50%, 25% or 0% of the water applied to controls (RDI_50%, RDI_25%, and RDI_0%, respectively) from mid-June to the end of July (6 weeks). Fully irrigated trees acted as first controls while trees undergoing previously tested postharvest deficit irrigation (25% of water applied to controls; RDI_Long) from early June up to the end of August (13 weeks of RDI total) acted as second controls. All deficit irrigation treatments promoted earlier flowering when compared to fully irrigated trees; the greatest advancement in full bloom date (27 days) was achieved with severe short term RDI (RDI_0% and RDI_25%). The trees suffering an extended period of water stress advanced full bloom date but to a lesser extent (13 and 18 days; 2004/2005 and 2005/2006, respectively). Earlier bloom derived in an earlier harvest date without detrimental effects on fruit quality and productivity. In this regard, the most severe RDI (RDI_0%) advanced mean harvest date the most (7 and 9 days, depending on the season), and increased the percentage of precocious yield to the highest extent. Productivity was not diminished by reduced irrigation in either season. Fruit size and grading was enhanced thanks to RDI in both seasons. Earliness and better fruit class distribution under RDI also improved fruit value and gross revenue enabling farmers both to increase earning and economize on water.     

灌水处理对冬小麦生理生长特性等的影响研究

通过对冬小麦不同生育期进行有限灌溉的大田试验,研究不同水分处理对冬小麦的土壤水分变化、株高、叶面积指数、光合特性、产量以及水分利用效率的影响。结果表明:①灌水量越大,含水量变化的土层深度越大;并且土层深度越大,含水量的变化越小。②开花期灌水对缺水处理叶面积指数和株高的补偿效应明显。③各处理的光合特性日变化趋势一致,蒸腾速率和气孔导度日变化趋势均是双峰曲线,光合速率日变化趋势均是单峰曲线,均有"午休"现象。④开花水有利于籽粒的形成,只灌开花水也可获得较高产量。但产量与总灌水量之间呈抛物线关系。⑤单叶和产量水平的水分利用效率均随灌水量的不同而不同。总灌水量越大,叶片水分利用效率越大,灌溉水利用效率越低,水分利用效率越低。⑥综合考虑,全生育期只灌1次75 mm开花水的处理最合理。     

Effect of salinity on water stress, growth, and yield of maize and sunflower

Maize and sunflower were grown in tanks filled with loam and clay, and were irrigated with water of three different levels of salinity. Predawn leaf-water potential and stomatal conductance were used as parameters for water stress. The predawn leaf-water potential of maize was higher than that of sunflower, but the effect of salinity and soil texture on the predawn leaf-water potential was the same for both crops. The stomatal conductance of sunflower was much higher and more severely affected by salinity and soil texture than the stomatal conductance of maize.

Although salinity had a more serious effect on the development of leaf area and canopy dry matter of sunflower, its effect on evapotranspiration and grain yield was the same for both crops. Soil texture had a stronger effect on the development of leaf area and canopy dry matter of sunflower, which also appeared in the evapotranspiration and grain yield, indicating that sunflower is more sensitive to drought than maize.     

水分和锌对番茄叶水势、光合特性及蒸腾效率的影响

通过盆栽试验,研究了3个土壤水分水平W1、W2、W3和2个锌溶液水平Z0、Z1对番茄叶水势、光合特性及水分利用效率的影响。结果表明,随着土壤水分的增加,番茄叶水势升高,晴朗天气中午12:00时W3处理叶水势可达-1.52 MPa;W3处理叶面积、光合速率、蒸腾速率和气孔导度分别比W1与W2高出25.7%与10.0%、19.6%与4.2%、20.7%与9.3%、26.6%与15.8%;叶面喷施锌不同程度的提高了叶片光合速率、蒸腾速率和气孔导度,在W2适宜水分下,三者分别提高9.1%、2.9%和7.3%;土壤水分的提高使植株的蒸腾效率增加,在W1、W2条件下叶面喷锌显著提高了叶片蒸腾效率。     

Photosynthesis,plant growth and dry matter distribution in kiwifruit as influenced by water deficits

The effects of water deficits on photosynthesis, plant growth and dry matter accumulation and distribution in the kiwifruit, cv Hayward, grown under controlled conditions in the glasshouse were studied. Water stress was imposed by irrigating the plants with 100%, 85%, 65% and 40% of water needed to reach pot capacity in the soil. Water deficits reduced the rate of photosynthesis by up to 53–64% in relation to the control. This decline was attributed to stomatal closure, since stomatal conductance was reduced significantly, or/and to inhibition of photosynthesis at chloroplast level. Severe water stress reduced plant height by 78–84%, total dry weight by 58–66% and total leaf area by 72–77%. The root to shoot ratio was 3.5 times higher in water-stressed plants, showing that water stress in kiwifruit alters the pattern of dry matter distribution favouring the roots. The decrease in growth induced by water deficits was a consequence of a reduction in both photosynthesis and photosynthates partitioning, which adversely affects leaf area development.