秸秆还田方式对丛枝菌根真菌群落和玉米磷素利用的影响

丛枝菌根真菌（arbuscular mycorrhizal fungi,AMF）与80%左右的植物可形成共生结构,在农田生态系统中对植物—土壤系统养分循环起重要作用。为改善我国南方典型瘠薄红壤加速酸化、磷有效性低和土壤生物功能退化等严重问题,采用高通量测序技术揭示秸秆还田方式对根际AMF群落、土壤磷酸酶活性和磷素利用率的作用机制。结果表明,不同秸秆还田方式显著影响土壤理化性质,其中秸秆猪粪配施处理显著提升有机碳（SOC）、全氮（TN）、全磷（TP）、有效磷（AP）以及酸性磷酸酶（acid phosphomonoesterase,ACP）和碱性磷酸酶（alkaline phosphomonoesterase,ALP）活性。秸秆还田显著影响AMF多样性和群落结构,球囊霉属（Glomus）和类球囊霉属（Paraglomus）是AMF群落的优势属,SOC是影响AMF多样性和群落结构的关键因子。秸秆猪粪配施处理对玉米磷素利用率的提升效果最佳,显著高于秸秆还田和秸秆生物质炭处理。AP、TP、ACP活性和AMF群落多样性显著影响了磷肥利用率。不同秸秆还田方式下根际AMF群落可能调控了土壤磷素活化过程...     

丛枝菌根和根瘤菌双接种对矿区土地复垦的生态效应

通过盆栽试验研究了山西省襄垣县矿区复垦土壤中接种丛枝菌根真菌和根瘤菌对三叶草的生长状况及土壤养分的影响.结果表明,双接种丛枝菌根和根瘤菌能显著提高菌根侵染率和土壤中孢子密度,促进三叶草干物质的积累和对氮、磷元素的吸收,促进三叶草对土壤养分的活化,提高土壤养分利用率,加速矿区生态恢复.     

土壤中丛枝菌根真菌对宿主植物磷吸收作用机制综述

由于贫瘠土壤不能供给植物足够磷素,而丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)在促进植物生长和吸收利用磷方面发挥着重要的作用。评述了国内外丛枝菌根真菌吸收土壤磷、根际环境与AMF共同作用对土壤磷的影响、AMF储磷机理、磷从菌丝到根系转移和植物吸收利用磷机制的研究进展,为今后应用AMF改善土壤肥力和深刻了解接菌土壤中磷的迁移转化规律奠定基础。     

菌剂与肥料配施对矿区复垦土壤白三叶草生长的影响

采用盆栽试验研究了矿区复垦土壤菌剂与肥料的不同配施对白三叶草(Trifolium repens Linn)生长的影响。结果表明: 双接种VA 菌根真菌(Glomus mossea)和根瘤菌(Rhizobium)能显著提高白三叶草根瘤数、根瘤鲜重和固氮酶活性, 根瘤数在有机肥双接种与无机肥双接种处理之间差异不显著, 而根瘤鲜重和固氮酶活性差异显著; 肥料与各菌剂组合处理中, 有机肥双接种处理的白三叶草分枝数、干物质重最大; 在白三叶草生长40 d 和150 d 时, 双接种处理的叶片数均为各处理中最大值; 接种VA 菌根真菌、根瘤菌和双接种均可增加白三叶草根系的菌根侵染率和土壤孢子数, 总体表现为双接种处理＞接种VA 菌根真菌＞接种根瘤菌, 有机肥相应处理＞无机肥相应处理＞对照; 肥料与菌剂的配合施用可有效提高植物对土壤氮、磷、钾养分的吸收。在矿区复垦土壤上有机肥与VA 菌根真菌和根瘤菌菌剂配施能显著促进白三叶草的生长, 是提高矿区复垦土壤植被恢复中比较适宜的组合方式。     

磷肥对酸性红壤上玉米不同部位丛枝菌根真菌群落的影响

为了研究不同磷肥水平下酸性红壤上玉米不同部位丛枝菌根真菌(Arbuscular mycorrhizal fungi, AMF)群落多样性和组成结构,明确玉米不同部位AMF群落的分布特征及对磷肥的响应差别,为提高酸性红壤磷素利用提供理论依据。本实验设置三个磷肥水平：不施磷、低磷(25 mg P /kg)和高磷(100 mg P /kg),玉米培养4周后,测定玉米生物量和土壤理化性质,利用高通量测序技术检测玉米根部、根际和非根际土壤AMF群落结构和多样性。结果显示,随着磷肥水平增加,玉米生物量显著提高,高磷处理下玉米地上部磷含量显著高于不施磷和低磷处理。取样部位(根部、根际和非根际)显著影响了AMF群落优势属球囊霉属(Glomus)、巨孢囊霉属(Paraglomus)和近明球囊霉属(Claroideoglomus)相对丰度,但是磷肥影响不显著。类似的,取样部位而不是磷肥显著影响了AMF群落香农指数和物种丰富度。非度量多维标度(NMDS)结果显示,根部样品与非根际和根际土壤样品群落距离更远,而相同取样部位中不同磷肥水平间群落组成更为相似；置换多元方差分析(PERMANOVA)进一步表明,取样部位而不是磷肥显著影响了AMF群落组成结构,主要表现在根部样品与根际和非根际土壤不同。因此,酸性红壤上玉米不同部位对AMF群落的影响明显高于磷肥作用,表明AMF应用于酸性红壤时应重点考虑作物部位的特性。     

接种AMF提高干旱胁迫下土壤微生物活性和燕麦抗旱能力

【目的】研究接种AMF (arbuscular mycorrhiza fungi)提高干旱胁迫下土壤微生物生物量和酶活性，及提高土壤养分有效性的效果，为旱作区作物生产提供菌根调控技术。【方法】盆栽试验连续进行了两年，供试燕麦品种为‘坝莜18号’，栽培基质为生土与河沙以3∶1混合，每盆添加N 0.315 g、P₂O₅ 0.087 g。试验设两个土壤相对含水量(田间持水量的75%和55%)，并分别设接种AMF和不接种AMF处理，共4个处理。在燕麦抽穗期取样测定土壤微生物特性指标和养分含量，及燕麦各器官氮、磷积累量；成熟期测定燕麦籽粒产量。【结果】接种AMF条件下，55%土壤相对含水量处理的燕麦根系AMF侵染率比75%土壤相对含水量处理显著降低23.28%～33.35%。与正常水分条件相比，干旱胁迫显著降低了土壤微生物生物量碳和酶活性，降低了土壤有效氮、磷含量，pH和有机碳含量升高。干旱胁迫下，与未接种AMF相比，接种AMF显著提升了土壤微生物生物量碳、氮和磷含量，提高了土壤蔗糖酶、脲酶和碱性磷酸酶活性，速效磷含量显著增加了4.92%～5.41%，...     

发酵牛粪对苏丹草根系丛枝菌根（AM）真菌侵染及磷吸收效率的影响

通过温室盆栽试验,研究接种苏格兰球囊霉(Glomus caledonium)条件下添加不同比例发酵牛粪(0.33%、0.50%和1.00%)对苏丹草(Sorghum sudanense)根系丛枝菌根(AM)真菌侵染率、土壤孢子密度、植株生物量与根冠比及根系磷(P)吸收效率的影响。结果发现,与对照相比,接种AM真菌处理植株地上部生物量趋于下降、根冠比显著提高(p<0.05),在此基础上添加0.33%或0.50%发酵牛粪处理土壤孢子密度、植株根系生物量和AM真菌侵染率均趋于升高,根冠比没有明显变化,根系P吸收效率显著提高(p<0.05);添加1.00%发酵牛粪显著提高土壤孢子密度、植株生物量和根系AM真菌侵染率(p<0.05),根冠比与仅接种AM真菌处理相同,根系P吸收效率则达到仅接种AM真菌处理的1.83倍。结果表明,添加1.00%发酵牛粪对苏格兰球囊霉扩繁及其宿主植物P吸收均具有突出促进作用。     

接种AM真菌对甘薯光合作用及碳磷代谢酶活性的影响

为探讨丛枝菌根真菌和磷水平对甘薯生长特性的影响,采用盆栽试验方法,设置3个P水平(P_0,P_(50),P_(150)mg/kg),研究了接种AM真菌对甘薯生长、光合特性和叶片酶活性的影响。结果表明:接种AM真菌显著增加了甘薯根系侵染率、丛枝丰度、根内菌丝丰度和泡囊丰度。不同磷水平间甘薯的侵染率、丛枝丰度均差异显著,中磷的总体侵染情况显著高于低磷和高磷水平(P0.05)。低磷和中磷条件下,接种处理显著提高了甘薯的生物量和氮磷吸收量(P0.05),其中在磷50mg/kg水平下,接种菌根真菌后甘薯氮磷养分吸收量显著高于未接种处理,地上地下部生物量分别提高了28.6%和73.3%,而高磷条件下接种处理甘薯地上和地下部的生长显著降低。在低磷和中磷水平下,接种AM真菌显著提高了甘薯的净光合速率、气孔导度和蒸腾速率;在中磷水平下接种AM真菌甘薯叶片的蒸腾速率和气孔导度达到最大值,之后随着磷水平的升高而降低;当土壤磷素供应过高时,接种AM真菌属非气孔限制因素导致的光合速率降低(P0.05)。在低磷和中磷水平下,接种菌根真菌显著提高了甘薯叶片中蔗糖合成酶、6-磷酸葡萄糖酸脱氢酶、蔗糖磷酸合成酶和磷酸酶的活性;在高磷水平下,接种后甘薯叶片代谢酶活性明显降低。不同磷水平下的菌根效应表现为P_(50)P_0P_(150),说明接种菌根的效果受土壤磷水平的影响。     

丛枝菌根对芘污染土壤修复及植物吸收的影响

采用温室盆栽试验方法,研究了两种丛枝菌根真菌Glomus mosseae和 Glomus etunicatum对三叶草(Trifolium subterraneum L.)和辣椒（Capsicum annuum L.）修复芘污染土壤的影响。供试土样中芘初始浓度为0 ~ 75.18 mg/kg。结果表明,接种AMF可促进供试植物对土壤中芘的吸收,并且显著提高三叶草根的芘含量、根系富集系数、根和茎叶的芘积累量,但对辣椒根和茎叶芘含量、根系富集系数的影响不显著,这主要与植物的菌根侵染率和“菌根依赖度”不同有关。接种AMF土壤中芘的削减率高于普通植物修复,但植物吸收积累对修复的贡献率小于0.2%;因此推测,AM作用下良好的根际环境对土壤微生物数量和活性的提高、进而对土壤中芘降解的促进可能是菌根修复的主要机理。     

多样化培肥模式调控丛枝菌根真菌促进玉米磷素吸收

利用丛枝菌根真菌（Arbuscular mycorrhizal fungi，AMF）从农田生态系统获取养分，是红壤作物吸收土壤磷（P）素的有效途径。针对我国南方红壤生物功能退化、P素生物有效性低、作物产量低等问题，如何调控作物根际AMF群落，优化其与宿主的互惠共生关系，是打破红壤区作物P摄取瓶颈的关键。本研究结合红壤旱地生态间作与有机（秸秆、猪粪、生物肥）无机肥料配施的4种多样化培肥措施，基于作物产量和红壤磷素活化水平，筛选最优培肥模式。进一步利用扩增子高通量测序和显微观察等技术，解析红壤旱地最优培肥措施调控AMF群落组成，揭示优化的AMF群落激发宿主玉米P素摄取机理。结果表明，花生/玉米间作结合秸秆/生物肥的有机无机配施（In+NPKSB）相较于其他培肥方式使红壤旱地全磷（TP）提高29.07%、有效磷（AP）提升1.35倍，且增强了玉米根内AMF群落科水平间的联系。该措施AMF定殖率是传统培肥措施的2.24倍，提高玉米根际酸/碱性磷酸酶（ACP/ALP）活性32.18%和41.66%，玉米生物量提高34.98%，产量提高67.27%。本研究证实红壤旱地花生/玉米生态间作结合秸秆/生物肥有机无机配施的培肥措施可通过优化玉米根内AMF群落组成，促进土壤P素活化，为在红壤旱地因地制宜推广可持续农业发展的集成应用提供理论依据。     

Inoculating maize fields with earthworms (Aporrectodea trapezoides) and an arbuscular mycorrhizal fungus (Rhizophagus intraradices) improves mycorrhizal community structure and increases plant nutrient uptake

Earthworms and arbuscular mycorrhizal fungi (AMF) are important macrofauna and microorganisms of the rhizosphere. The effect of the inoculation of soil with earthworms (Aporrectodea trapezoides) and mycorrhiza (Rhizophagus intraradices) on the community structure of mycorrhizal fungi and plant nutrient uptake was determined with split plots in a maize field. Maize plants were inoculated or not inoculated with AMF, each treated with or without earthworms. Wheat straw was added as a feed source for earthworms. Inoculating AMF significantly increased maize yield (p?<?0.05), and the yield was further enhanced by the addition of earthworms. Alkaline phosphomonoesterase activities, soil microbial biomass carbon (SMBC) and nitrogen (SMBN) increased with the addition of both earthworms and AMF. Soil inorganic N and available K were positively affected by earthworms, while available P showed a negative relationship with AMF. Treatment with both AMF and earthworms increased shoot and root biomass as well as their N and P uptake by affecting soil phosphomonoesterase and urease activities, SMBC, SMBN, and the content of available nutrients in soil. The applied fungal inoculants were successfully traced by polymerase chain reaction with novel primers (AML1 and AML2) which target the small subunit rRNA gene. The amplicons were classified by restriction fragment length polymorphism and sequencing. Moreover, field inoculation with inocula of non-native isolates of R. intraradices appeared to have stimulated root colonization and yield of maize. Adding earthworms might influence native AMF community, and the corresponding abundance increased after earthworms were inoculated, which has positive effects on maize growth.     

Effects of microplastic polystyrene, simulated acid rain and arbuscular mycorrhizal fungi on Trifolium repens growth and soil microbial community composition

Microplastic pollution is a global and ubiquitous environmental problem in the oceans as well as in the terrestrial environment. We examined the fate of microplastic polystyrene (MPS) beads in experimental soil in the presence and absence of symbiotic arbuscular mycorrhizal fungi (AMF) and simulated acid rain (SAR) to determine whether the combinations of these three factors altered the growth of white clover Trifolium repens. We found that MPS, SAR, or AMF added singly to soil did not alter T. repens growth or yields. In contrast, MPS and AMF together significantly reduced shoot biomass, while SAR and MPS together significantly reduced soil available phosphorus independent of AMF presence. Microplastic polystyrene, AMF, and SAR together significantly reduced soil NO^-₃-N. Arbuscular mycorrhizal fungi added singly also enriched the beneficial soil bacteria (genus Solirubrobacter), while MPS combined with AMF significantly enriched the potential plant pathogenic fungus Spiromastix. Arbuscular mycorrhizal fungi inoculation with MPS increased the abundance of soil hydrocarbon degraders independent of the presence of SAR. In addition, the abundance of soil nitrate reducers was increased by MPS, especially in the presence of AMF and SAR. Moreover, SAR alone increased the abundance of soil pathogens within the fungal community including antibiotic producers. These findings indicate that the coexistence of MPS, SAR, and AMF may exacerbate the adverse effects of MPS on soil and plant health.     

南美蟛蜞菊、蟛蜞菊和杂交蟛蜞菊土壤磷组分特征及其影响因素

为探究入侵植物南美蟛蜞菊土壤磷转化过程与驱动机制,采用野外控制试验,比较研究了南美蟛蜞菊、蟛蜞菊和杂交蟛蜞菊土壤磷组分、微生物生物量磷以及酸性磷酸酶和碱性磷酸酶活性.结果表明:3种蟛蜞菊土壤易分解态磷仅占全磷的2.2％～6.3％,且全碳与有机磷的比例大于200,表明本研究区受到磷限制.有机磷是土壤磷库的主要组分,在南美...     

Arbuscular mycorrhizal fungi improve the growth and phosphorus uptake of mung bean plants fertilized with composted rock phosphate fed dung in alkaline soil environment

Abstract

Inoculation effect of arbuscular mycorrhizal fungi (AMF) on phosphorus (P) transfer from composted dung of cattle with a diet supplemented with powdered rock phosphate (RP) and their successive uptake by mung bean plants was assessed in alkaline soil. The efficacy of composted RP fed dung alone or/and in combination with AMF inoculums containing six different species were compared with SSP in six replicates per treatment in pots. The results showed that the association of AMF with composted RP fed dung had a positive effect on mung bean shoot (3.04?g) and root (2.62?g) biomass, chlorophyll (a, b), carotenoid contents and N (58.38?mg plant^?1) and P (4.61?mg plant^?1) uptake. Similarly, the percent roots colonization (56%) and nodulation of mung bean plant roots and their post-harvest soil properties were also improved by the inoculation of AMF together with composted RP fed dung. It is concluded that the combined application of AMF with composted RP fed dung has almost the same effect as SSP for improving mung bean plants growth and their nutrients uptake. Moreover, AMF inoculants can be used as a suitable biofertilizer in combination with locally available organic sources of fertilizers for improving P status and growth of plants in alkaline soils.     

蚯蚓与菌根提高玉米生长和氮磷吸收的互补效应

【目的】蚯蚓和丛枝菌根真菌处于不同的营养级,但在促进植物生长和提高土壤肥力等方面却都发挥着积极作用。研究蚯蚓菌根互作及其对玉米吸收土壤中的氮、磷养分的影响,可为提升土壤生物肥力及促进农业的可持续发展提供理论依据。【方法】本研究采用田间盆栽方式,以玉米为供试作物,研究蚯蚓(Eisenia fetida)与丛枝菌根真菌(Glomus intraradices)互作及其对玉米养分吸收的影响。试验设置P 25和175 mg/kg两个水平。每个磷水平进行接种与不接种菌根真菌以及添加与不添加蚯蚓,共8个处理。调查了玉米生长、养分吸收以及真菌浸染和土壤养分的有效性。【结果】两个磷水平下,蚯蚓和菌根在增加玉米地上部和根系生物量方面有显著正交互作用(P0.05)。接种菌根真菌的各处理显著增加了玉米的侵染率及泡囊丰度、根内菌丝丰度等菌根指标。同时添加蚯蚓和接种菌根真菌的处理(AM+E)显著提高了菌根的侵染率、菌丝密度、丛枝丰度和根内菌丝丰度但是泡囊丰度有所下降。两种磷水平下,AM+E处理玉米地上部和地下部含氮量和含磷量均显著高于其他三个处理。在低磷条件下,地上部氮磷总量的增加分别是添加蚯蚓和接菌的作用;而地下部磷总量的增加主要是菌根真菌的作用。在高磷条件下,单加蚯蚓显著增加玉米氮磷的总量,而接种菌根真菌对玉米氮磷吸收的影响未达显著性水平。在高磷条件下,单加蚯蚓的处理显著提高玉米地上地下部生物量(P0.05),而单接菌的处理效应不显著,蚯蚓菌根互作通过提高土壤微生物量碳、氮实现对玉米生长和养分吸收的调控。在低磷条件下,单接菌显著提高了玉米的生物量(P0.05),单加蚯蚓的处理具有增加玉米生物量的趋势。菌根真菌主要促进玉米对磷的吸收,蚯蚓主要矿化秸秆和土壤中的氮磷养分增加土壤养分的有效性,蚯蚓菌根互作促进了玉米根系对土壤养分的吸收并形成氮磷互补效应。【结论】无论在高磷还是低磷水平下,蚯蚓菌根相互作用都提高了玉米地上地下部生物量、氮磷吸收量同时提高了土壤微生物量碳、氮。蚯蚓菌根相互作用对植物生长的影响取决于土壤养分条件。在高磷条件下(氮相对不足),蚯蚓菌根互作通过调控土壤微生物量碳、氮调控玉米生长和养分吸收。低磷条件下,菌根主要发挥解磷作用,蚯蚓主要矿化秸秆和土壤中的氮素,蚯蚓和菌根互补调控土壤中氮、磷,从而促进植物的生长和养分吸收。     

Additive and interactive effects of functionally dissimilar soil organisms on a grassland plant community

The productivity and diversity of plant communities are affected by soil organisms such as arbuscular mycorrhizal fungi (AMF), root herbivores and decomposers. However, it is unknown how interactions between such functionally dissimilar soil organisms affect plant communities and whether the combined effects are additive or interactive. In a greenhouse experiment we investigated the individual and combined effects of AMF (five Glomus species), root herbivores (wireworms and nematodes) and decomposers (collembolans and enchytraeids) on the productivity and nutrient content of a model grassland plant community as well as on soil microbial biomass and community structure. The effects of the soil organisms on productivity (total plant biomass), total root biomass, grass and forb biomass, and nutrient uptake of the plant community were additive. AMF decreased, decomposers increased and root herbivores had no effect on productivity, but in combination the additive effects canceled each other out. AMF reduced total root biomass by 18%, but decomposers increased it by 25%, leading to no net effect on total root biomass in the combined treatments. Total shoot biomass was reduced by 14% by root herbivores and affected by an interaction between AMF and decomposers where decomposers had a positive impact on shoot growth only in presence of AMF. AMF increased the shoot biomass of forbs, but reduced the shoot biomass of grasses, while root herbivores only reduced the shoot biomass of grasses. Interactive effects of the soil organisms were detected on the shoot biomasses of Lotus corniculatus, Plantago lanceolata, and Agrostis capillaris. The C/N ratio of the plant community was affected by AMF.In soil, AMF promoted abundances of bacterial, actinomycete, saprophytic and AMF fatty acid markers. Decomposers alone decreased bacterial and actinomycete fatty acids abundances but when decomposers were interacting with herbivores those abundances were increased. Our results suggests that at higher resolutions, i.e. on the levels of individual plant species and the microbial community, interactive effects are common but do not affect the overall productivity and nutrient uptake of a grassland plant community, which is mainly affected by additive effects of functionally dissimilar soil organisms.     

低磷环境下接种丛枝菌根真菌促进紫花苜蓿生长和磷素吸收的机理

[目的]磷极易被土壤吸附和固定,导致土壤中磷有效性较低.研究接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)和低磷处理两者交互对紫花苜蓿生长和磷吸收的影响,为提高碱性土壤中磷肥利用率提供理论依据.[方法]以黄绵土和紫花苜蓿(Medicago sativa)为试验材料进行盆栽试验.在施...     

土壤磷酸酶活性对施肥-种植-耕作制度的响应

植物所需的磷素营养,主要来源于土壤内源、外源无机磷。而大量存在的有机磷类化合物在土壤磷酸酶的作用下催化水解为无机磷,不仅是土壤内源无机磷的主要来源,也是土壤磷素循环的一个重要的环节。土壤磷酸酶在土壤中主要以吸附态的形式存在,其活性受到施肥、种植和耕作制度的影响。本文对国内外不同施肥方式、种植及耕作制度下土壤磷酸酶(酸性磷酸酶、中性磷酸酶、碱性磷酸酶、磷酸二酯酶、磷酸三酯酶、焦磷酸酶)活性响应进行综述,拟为不同农业管理制度下土壤磷酸酶活性的调节提供依据。     

Colonization, ALP activity and plant growth promotion of native and exotic arbuscular mycorrhizal fungi at low pH

Soil acidity can affect arbuscular mucorrhizal fungi (AMF) in many aspects. In this study, by inoculating white clover (Trifolium ripens L.) with AMF at two pH levels (pH 5.0 and 6.0), we investigated the influences of low pH on the colonization, alkaline phosphatase (ALP) activity and promoting effect of AMF, and also compared the different responses of native AMF community and exotic species (Gigaspora margarita) to low pH. PCR-DGGE profiles revealed the obvious difference in native AMF community structure between low and high pH. Compared to low pH, high pH decreased the total and vesicle colonization, external hyphae length density of native AMF, but exerted no effect on those of exotic AMF. For both native and exotic AMF, high pH enhanced the proportions of arbuscule and fungal structure with ALP activity in colonized roots. Both native and exotic AMF promoted the shoot biomass, with greater increase at low pH than at high pH. Plant growth was more dependent on exotic AMF than on native AMF at both pH levels. The same trends were observed in P uptake as in biomass. Our study suggested that the increase in pH can exert inhibitory effects on AMF species native to acidic soil, including ecological (community structure), functional (ALP activity) and developmental (hyphal length, vesicle and arbuscule percentage, total colonization) aspects, but have no effect on exotic species in the present experiment.     

分室磷添加下菌根对滇池流域红壤间作玉米生长及磷素利用的影响

随着全球范围内磷矿资源短缺问题的日益严重,间作或菌根技术强化作物对土壤磷(P)的利用及增产增收的效应受到越来越多的关注。通过三室隔网盆栽模拟试验研究了分室磷处理[不添加磷(P0)、添加有机磷(OP50)、添加无机磷(IOP50)]和根室不接种(NM)、根室接种丛枝菌根真菌Glomus mosseae(GM)对与大豆间作的玉米的生长及磷素利用的影响。研究结果表明:所有复合处理中,以间作?GM?IOP50组合处理下的玉米根系最短和地上部生物量最高;OP50处理下,间作玉米的菌根侵染率显著高于单作处理。间作条件下,无论分室磷添加与否,接种GM处理的玉米地上部生物量明显高于NM处理;接种GM处理的玉米根系生物量和株高均显著高于NM处理,且根系生物量以间作?GM?OP50组合处理下最高。接种GM条件下,P0、IOP50、OP50处理下的间作植株生物量较单作处理分别提高45.98%、111.33%、33.56%。单作条件下,无论分室磷添加与否,接种GM处理的玉米地上部磷含量均显著高于NM处理;无论何种种植模式及分室磷添加与否,接种GM处理的植物根系磷含量均显著高于NM处理。无论磷添加与否,间作?GM组合条件下的玉米地上部磷吸收量均显著较高,其中IOP50处理下的地上部磷吸收量显著高于OP50处理。间作?GM组合条件下,IOP50处理玉米根系的磷吸收效率均显著高于OP50处理。可见,接种GM、分室磷添加和间作各自在一定程度上促进了玉米的生长。综合菌根侵染、生物量及磷含量与吸收量、磷吸收效率等指标,所有复合处理中以间作?GM?IOP50组合对玉米地上部的促生作用最好,玉米磷素吸收最多,可望有效强化滇池流域红壤坡耕地磷素的利用。