湖南省部分地区棉田牛筋草(Eleusine indica)对精喹禾灵的抗性

采用整株法测定了湖南省部分地区棉田牛筋草(Eleusine indica)对精喹禾灵的抗性水平,还测定了精喹禾灵对牛筋草抗(R)、感(S)种群体内GSTs及SOD、POD、CAT影响的差异。整株法测定结果显示,相对于敏感的南县种群,其他地区棉田牛筋草对精喹禾灵的抗性指数在1.6~9.7之间,其中湘阴种群最高为9.7,澧县种群最低为1.6;精喹禾灵处理2d后,R种群GSTs活性急剧上升,在第3天达到最高,随后急剧下降,5d之后趋于平缓。S种群GSTs活性在施药后先上升后下降。药剂处理后,R种群的SOD和POD活性在中期显著低于S种群,R、S种群的CAT活性在药后第9天有明显差异。结果表明,R种群对精喹禾灵产生抗性的原因可能是GSTs对精喹禾灵的代谢作用加强,抗氧化酶系活性的变化可能与抗性相关。     

牛筋草生防菌NJC-16的分离鉴定及生物学特性研究

为了筛选并获得低毒且高效的牛筋草生防菌,采用常规组织分离法获得牛筋草炭疽病病原菌菌株NJC-16,通过形态学观察结合ITS序列分析对菌株进行鉴定,确定该菌株为牛筋草炭疽菌Colletotrichum eleusines.该病原菌最适生长温度为25℃;不同光照条件对病原菌生长无显著差异;在供试碳(氮)源中病原菌对淀粉和酵...     

抗草甘膦杂草检测方法的研究进展

目前,抗草甘膦杂草问题日趋严重。通过检索国内外抗草甘膦杂草的检测方法,总结出常用检测方法和其他检测方法,进行了简单概述,为抗草甘膦杂草检测体系的发展提供依据。     

美国草甘膦抗药性日益严重

去年,市场研究公司Stratus农产品市场营销(圭尔夫,安大略省)的调查指出,美国和加拿大的近一半(49%)的美国农民表示在他们的农场存在有抗草甘膦除草剂药性的杂草。与此相比,2011年调查的有34%的农民表示有此现象。过去三年该公司就抗药性问题对横跨美国31个州上万名美国农民进行了调查。     

抗草甘膦杂草及其检测方法发展现状

草甘膦在世界范围的多年大量使用已经引起了抗草甘膦杂草的产生。本文针对全球迄今为止发现的21种抗草甘膦杂草的发生、发展状况进行了论述。探讨了抗草甘膦杂草抗药性检测方法,分别从整株生物测定及生物化学等方面介绍了抗草甘膦杂草检测方法的研究现状,为抗草甘膦杂草检测方法的发展及其抗性监测方法的建立提供参考。     

外来杂草非洲牛筋草的形态特征研究

本文记述了一种口岸常规监测中发现并及时铲除的外来杂草——非洲牛筋草（Eleusine africana Kenn-O’Byrne）,阐述了其分类地位、分布和起源、形态特征及潜在风险等,并将其与近似种牛筋草（E. indica）和穇子（E. coracana）进行了形态比较区分,以便于识别。     

草甘膦应用技术研究

目前草甘膦应用浪费问题严重 ,本文对国内外草甘膦应用技术方面的研究 ,包括施药最适期的选择 ,施药技术 ,发挥最佳药效的环境条件 ,合理混用技术等进行了论述 ,以其得到更经济、更广泛的应用。     

不同生育期转基因抗草甘膦抗虫棉花对草甘膦的耐受性

为明确转基因抗草甘膦抗虫棉田草甘膦的使用适期,于棉花子叶期、3~4片真叶期和花铃期喷施不同浓度的草甘膦,比较药害发生情况、棉花产量和纤维品质。棉花子叶期、3~4片真叶期和花铃期喷施1 640~9 840 g/hm²(有效成分,下同)草甘膦后,施药初期棉花均表现出一定的药害症状,药后4~8 d药害株率和药害指数达最大值,药后12 d药害症状逐渐减轻或消失。棉花子叶期和3~4片真叶期喷施草甘膦,对棉花产量无显著影响,但棉花花铃期喷施2 460~9 840 g/hm²草甘膦后,棉花产量显著降低,降幅达35.0%~63.3%。3个时期喷施草甘膦均不影响棉花的纤维品质。研究表明,转基因抗草甘膦抗虫棉田草甘膦的使用适期为棉花苗期。     

马唐对烟嘧磺隆的抗药性研究

2008—2009年在我国东北、华北的6个省份烟嘧磺隆防效下降的玉米田块采集80个马唐种群,在可控温室中采用整株测定法对其进行抗性检测试验。结果表明,不同用药历史玉米田的马唐种群对烟嘧磺隆已经产生了不同程度的抗药性,其中有10个种群产生的抗药性较显著。     

牛筋草ISSR-PCR反应体系的建立与优化

以牛筋草地上组织为材料,采用正交优化和单因子试验两种方法对影响牛筋草ISSR-PCR体系的Mg2+浓度、dNTP浓度、Taq DNA聚合酶浓度、引物浓度和DNA模板浓度5个因素进行优化试验,建立适合牛筋草ISSR-PCR的反应体系.结果表明,牛筋草ISSR-PCR反应体系的最佳条件为:Mg2+1.75 mmol/L,dNTP 0.4 mmol/L,Taq DNA聚合酶1 U/25 μL,引物0.3μmol/L,模板DNA 80 ng/25μL,10×PCR Buffer 2.5 μL/25 μL.利用优化体系进行牛筋草的ISSR-PCR反应,可获得稳定性高、重复性好、背景清晰的电泳结果.     

Changes in seed bank size and dormancy characteristics of the glyphosate-resistant biotype of goosegrass (Eleusine indica[L.] Gaertn.)

A study on changes in the seed bank size of a glyphosate ( N -(phosphonomethyl)glycine)-resistant (R) Eleusine indica biotype was carried out on a naturally occurring infestation of a young oil palm plantation. The total number of shed seeds collected was 53% of the predicted potential total number of seeds produced by the plants. Ametryn ( N -ethyl- N' -(methylethyl)-6-(methylthio)-1,3,5-triazine-2, 4-diamine) was found to be able to control the R seed population in the soil. The periodicity of seedling emergence was influenced by rainfall subsequent to the 1 month period of seed shedding. Seed burial at 2, 10 and 20 cm depths showed that 39, 33 and 79%, respectively, of viable seed persisted in the soil after 2 years. Seeds in the state of enforced dormancy played an important role in maintaining the seed population throughout the 2 year period of burial in the soil.     

Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia

Summary Resistant (R) and susceptible (S) biotypes of Eleusine indica were collected from four areas, namely Chaah, Lenggeng, Bidor and Temerloh, in Malaysia. Restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR)-RFLP analyses using Sph I restriction enzyme were able to differentiate the R biotype from the S biotype by showing R-specific and S-specific polymorphisms in E. indica from three of the areas, with the exception of Temerloh where no polymorphisms were detected. The different DNA profiles for the R biotypes obtained indicate that Sph I is not a useful diagnostic marker. The DNA polymorphisms detected in the 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase gene suggest that there are different mutation events leading to development of resistance to glyphosate. Partial sequencing of the EPSP synthase gene confirmed different mutations occurring with substitution of proline with serine or threonine at amino acid 106 for the R biotype in Chaah, Bidor and Temerloh.     

Germination and seedling emergence of glyphosate‐resistant and susceptible biotypes of goosegrass (Eleusine indica[L.] Gaertn.)

Effects of environmental factors on the germination and seedling emergence of glyphosate‐resistant (R) and ‐susceptible (S) biotypes of Eleusine indica (L.) Gaertn. were examined under laboratory and greenhouse conditions. The R biotype exhibited a higher germination percentage compared with the S biotype at constant temperatures of 20 and 35°C under dark conditions, and alternating temperatures of 30/25°C, and 35/25°C during a 12 h photo period. For both biotypes, germination was optimal at alternating temperatures of 30/20°C and 35/20°C. However, there was no significant difference (P > 0.05) in the germination between the R and S biotypes at these temperature regimes. The germination of both biotypes was inhibited by osmotic stress imposed by a water potential of ?0.80 MPa. When the moisture stress was released and the seeds were subsequently transferred to distilled water, the germination was enhanced to approximately 90% and 16% for the R and S biotype seeds, respectively. Higher emergence rates were obtained in shallow seed depths (0 or 2 cm) compared to deep depths. Emergence percentage of the R biotype was higher than that of the S biotype at 0 cm and 2 cm depths. The maximum emergence percentage of the R biotype was higher than that of S biotype when seeds were sown on the surface of either loamy or clay loam soil taken from three different sites.     

外来入侵恶性杂草牛筋草的化学防除药剂评价筛选

牛筋草是全球危害最严重的5种杂草之一,为了筛选防除牛筋草的高效除草剂品种,采用温室盆栽法和田间小区试验测定了10种苗后除草剂对牛筋草的除草活性及防除效果。盆栽试验结果表明,30%苯唑草酮SC和40 g/L烟嘧磺隆OD对牛筋草防效优异,ED₉₀分别为8.7 g/hm~2和19.4 g/hm~2(有效成分,下同),约为各自登记低剂量的1/3和1/2。100 g/L氰氟草酯EC、10%噁唑酰草胺EC、69 g/L精噁唑禾草灵EW、120 g/L烯草酮EC和10%精喹禾灵EC对牛筋草具有较好的防效,ED₉₀分别为68.9、80.5、50.0、54.2 g/hm~2和48.6 g/hm~2,与各自的登记低剂量相当或略低。25%啶嘧磺隆WG和11%三氟啶磺隆OD对牛筋草的防效较差,ED₉₀分别为115.0 g/hm~2和112.4 g/hm~2,约为各自登记高剂量的1.5倍和2.3倍。75%硝磺草酮WG对牛筋草的防效很差,ED₉₀为742.6 g/hm~2,是其登记高剂量的3.3倍。田间试验结果表明,40 g/...     

Effects of nitrogen fertilization and cutting height on the forage yield and feeding value of Eleusine indica in the dry season in Nepal

The objective of this study was to determine the regrowth characteristics, crude protein (CP) content, and feeding value of Eleusine indica grass during the dry season in Nepal. The grass was cultivated using three different levels of N fertilization (0, 50, and 100 kg ha⁻¹) and cutting heights (2, 4, and 6 cm above the ground) in a 3 × 3 factorial design. The forage yield, number of tillers per plant, and CP content of the grass were determined. A digestibility trial was conducted with six local, female Khari goats (15 ± 1.7 kg body weight) in a cross-over design to compare the feeding value of E. indica with local forages. A comparatively higher forage yield, number of tillers per plant, and CP content were obtained with 100 kg N ha⁻¹. However, the forage yield and CP content were not significantly affected by the cutting height. With different levels of N fertilization and cutting heights, the cumulative forage yield in five harvests during the dry season (December to May) ranged from 1.83–3.82 t dry matter ha⁻¹. The digestibility coefficients of the dry matter, CP, crude fiber, and ether extract content of E. indica for goats were 0.54, 0.67, 0.70, and 0.54, respectively. The nutrient digestibility and palatability of E. indica were comparable to that of the mixed local forages. Hence, E. indica in conjunction with a sound N fertilization practise potentially can be utilized as an approach to overcome the problem of green forage scarcity, especially during the dry winter season in regions that have a tropical climate.     

Effects of trampling and ethephon on leaf morphology in trampling-tolerant Plantago asiatica and Eleusine indica

Summary The effects of simulated human trampling on leaf morphology and ethylene production as well as the effect of an ethylene releaser ethephon on leaf morphology were investigated for trampling-tolerant species, Plantago asiatica and Eleusine indica , and trampling-susceptible species, Gnaphalium affine and Panicum bisulcatum . In Pl. asiatica , trampling decreased the leaf length and increased the leaf blade length to width ratio and the leaf blade length to leaf (leaf blade + petiole) length ratio. In E. indica , trampling only decreased the leaf blade length to width ratio. Trampling increased ethylene production in intact seedlings of Pl. asiatica , E. indica and G. affine . The ethephon (1 mM) treatment increased the leaf blade length to width ratio in seedlings of Pl. asiatica , but decreased that in seedlings of E. indica in a manner similar to the morphological changes induced by trampling. In contrast, no significant differences in leaf morphology were observed between the ethephon-treated and control seedlings of trampling-susceptible species. These results suggest that trampling-tolerant species have higher responsiveness to ethylene than trampling-susceptible species and change their leaf morphology in response to ethylene induced by trampling.