丛枝菌根真菌对青稞生长发育及磷营养的影响研究

青稞是大麦类作物中的一种，是西藏地区栽培面积最大、产量最多、分布最广的农作物品种。近年来，青稞的保健作用（富含-β葡聚糖和“生育三烯酚”）已得到国内外公认，因而青稞生产受到更广泛的关注。然而，根据西藏首次土壤资源调查结果，全自治区低磷（速效磷〈10mgkg^-1）土壤占总耕地面积的46．9％～72．3％（内部资料）。土壤供磷不足是限制青稞高产的重要潜在因素之一，同时西藏目前没有一家化肥厂，生产、科研所需肥料全靠从内地长途运输。寻找一条经济有效的途径缓解西藏地区农业生产肥料紧缺的状况成为一项迫切的任务。     

菌根接种对柑桔磷素营养和生长效应的研究

许多研究证明菌根能显著地促进柑桔生长.Menge等(1975)报道接种Glomus fasciculatus于不同柑桔品种实生苗后,其植株生长量比无菌根的增加120—2600%.目前,美国、澳大利亚和南非等国以菌根真菌作为一种“生物肥料”,正研究菌根接种剂的商业生产,企图应用于柑桔果园,来提高肥效和减少施肥量.     

水磷耦合对冬小麦幼苗营养元素含量的影响

本文研究了速效水范围内水磷耦合对冬小麦幼苗的N、P、K吸收量的影响。结果表明:不同速效水水平对小麦苗期N、P和K的吸收有明显影响。在不同的P水平处理时最高N、P吸收值都出现在速效水高的处理上,低水时N、P吸收也较低。中P与高水配合有利于N、P的吸收。低速效水时提高P水平可促进P吸收,但不能显著提高N吸收。速效水的水平对K吸收影响不如对P吸收影响明显,品种间差别不大。结果还显示土壤低P处理显著降低植株幼苗对P素的吸收,不同速效水水平时增加土壤P可以提高植株吸P量。土壤缺P对小麦植株K吸收有一定影响,水磷耦合后品种间的K吸收量差异加大。     

PHOSPHORUS EFFECTS IN THE NUTRITION AND GROWTH OF DEVELOPING MANGO PLANTS

The objective was to study the effects of phosphorous (P) fertilization on nutritional and developmental aspects of growing mango plants. The mango plants were evaluated by soil chemical analyses, leaf chemical analyses, biological examination of plant growth, and the starting point of fruit production. Having this in view, an experiment was set up on 2 January 2003, at Flora, a farm in Uberlandia, state of Minas Gerais, Brazil. The soil was a clayish Oxisol. The doses at planting were: D₀ = zero, D₁ = 40, D₂ = 100, D₃ = 200, and D₄ = 300 g of P₂O₅ plant^?1. These doses at the beginning of the second year were multiplied by 1.5 and at the beginning of the third year by 2.0 and applied to the plants. The fertilizer used in this experiment was triple superphosphate (44% of P₂O_5.). During August of 2004, 2005, and 2006, soil samples were taken at a depth of 20 cm in between the plant rows. Leaf samples were taken during August of 2004 and 2005 to determine macro- and micronutrient contents in the leaves. Plant stem diameter was measured during January of 2004 and 2005. Plant height and crown radius were measured during January of 2005 and fruit production in 2005 and 2006. Fertilizer applications increased the level of P in the soil but significantly influenced plant performance only after the second year. The effects of phosphorus on mango plants take place slowly leading to increments in plant stem diameter only at the third year. Fruit set was not influenced by phosphorous fertilization.     

EFFECTS OF CONVENTIONAL AND CONTROLLED RELEASE PHOSPHORUS FERTILIZER ON CROP EMERGENCE AND GROWTH RESPONSE UNDER CONTROLLED ENVIRONMENT CONDITIONS

A series of experiments on the effects of form and rate of seed row placed phosphorus (P) fertilizer were carried out under controlled environment conditions using flats of a P-deficient Brown Chernozemic soil from Saskatchewan, Canada. The experiments were conducted in the laboratory and growth chamber using rates of seed row placed granular P fertilizer up to 100 kg P₂O₅ ha^?1. Two forms of monoammonium phosphate fertilizer were compared: 1) conventional MAP granules and 2) controlled release phosphorus (CRP) fertilizer granules (Agrium Inc, Denver, CO, USA.) made with a polymer coating to slow the release of phosphate to soil solution. Six crops were utilized in the study to provide a range of commonly grown cereal, oilseed, pulse and forage crops in Western Canada: wheat (Triticum aestivum), canola (Brassica napus), mustard (Brassica juncea), flax (Linum usitatissimum), yellow pea (Pisum sativum) and alfalfa (Medicago sativum). Parameters measured were percentage of planted seeds that had emerged after two weeks, plant biomass yield, and plant P uptake after four weeks. Most of the crops tested showed no negative impact on emergence with seed row placed conventional P fertilizer at rates up to ～20 to 30 kg P₂O₅ ha^?1. Pea, flax and mustard tended to be most sensitive to injury from high rates of seed placed MAP while wheat was least sensitive. The controlled release phosphorus fertilizer (CRP) product greatly increased the tolerance of crops to high rates of seed row placed P, with rates of 80 kg P₂O₅ ha^?1 placed in the seed row producing no significant injury for most crops. This effect is attributed to the coating reducing the harmful salt effect that occurs when high rates of fertilizer are placed in the seed row in close proximity to the seed. Generally, a rate of 30 kg P₂O₅ ha^?1 was sufficient to produce maximum early season biomass yield and P uptake for both conventional MAP and CRP fertilizers. Large differences in early P availability were not evident between the conventional P and controlled released P fertilizer products.     

METABOLIC CHANGE,TILLERING AND ROOT SYSTEM OF BRACHIARIA BRIZANTHA IN RESPONSE TO PHOSPHORUS AND ZINC NUTRITION

Phosphorus (P) and zinc (Zn) availabilities are important to the nutrition of tropical forage grasses. In this manner, this study aimed to identify and quantify changes in metabolism, tillering, and root system of Brachiaria brizantha cv. ‘Marandu’ related to P and Zn availabilities. Plants of B. brizantha cv. ‘Marandu’ were grown in nutrient solution under five rates of P (0.1, 0.6, 1.1, 1.6, and 2.1 mmol L^?1) and five rates of Zn (0.00, 0.75, 1.5, 2.25, and 3.00 μmol L^?1), in a fractionated factorial. The interaction P x Zn rates and the Zn supply were not significant for the activity of acid phosphatase enzyme, P-use index, number of tillers, and root parameters. P-use index and enzyme activity decreased as P availability increased. The high efficiency in P use was a result of high acid phosphatase activity and P supply was essential to the development of ‘Marandu’ palisadegrass root system.     

EFFECT OF VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI ON THE RELATIONS OF PLANT GROWTH, INTERNAL PHOSPHORUS CONCENTRATION AND SOIL PHOSPHATE ANALYSES

The effect of infection by vesicular-arbuscular mycorrhiza on the predictive value of soil analysis for available P was tested in a glasshouse experiment. Leeks (Allium porrum) were grown on ten Rothamsted soils with a wide range of initial NaHCO₃-soluble P, each of which also received 5 levels of added P. Soils were partially sterilized with 1 Mrad of γ-radiation, or left untreated; plants on sterilized soil were infected with yellow-vacuolate endophyte (Glomus mosseae) or left non-mycorrhizal. The NaHCO₃-soluble P in the soils was measured 5 days after phosphate addition. Yields from all P levels on all soils were plotted against soil NaHCO₃-soluble P content and separate smooth response curves were obtained for non-infected and artificially-infected plants. Infection increased yield only on soils with less than 100 mg P kg^?1. In contrast, naturally-infected plants gave no clearly defined response curve to P, and on three soils the yields were consistently low. These soils gave very low natural infections and had low spore densities probably due to the cropping history of the sites. Artificially-infected plants had much higher shoot P concentrations than did uninfected plants of similar dry weight, over a large range of soil phosphate levels; this effect was also noted with the endophyte G. fasciculatus. Naturally-infected plants showed a poor relationship between shoot P concentration and yield.     

EFFECT OF SOIL AMENDMENT WITH ALFALFA POWDERS AND DISTILLERS GRAINS ON NUTRITION AND GROWTH OF CANOLA

Two pot experiments were carried out under controlled environment conditions in the growth chamber to assess the potential use of alfalfa powders and distiller grains as organic fertilizers. Two types of dehydrated alfalfa powders (one with canola meal protein extraction by-product and one without) and two types of distiller grains (dried distillers grain with distillation solubles added and wet distillers grain without solubles) from wheat-based ethanol production were evaluated. Four different nitrogen (N)-based amendment application rates (0, 100, 200 and 400 kg N ha^?1) were used along with urea applications made at the same N rates to a Brown Chernozem (Aridic Haploboroll) loamy textured soil collected from south-central Saskatchewan, Canada. Canola biomass yield, N, phosphorus (P), potassium (K), zinc (Zn), and cadmium (Cd) uptake were measured along with soil properties including pH, salinity, organic carbon, total nitrogen, phosphorus and extractable nutrients and cadmium before and after canola growth in each of the treatments. Application of alfalfa powder and distiller grain amendments resulted in significant canola biomass yield increases along with increased N, P, and K uptake compared to the unfertilized control. However, only a portion of the N added (～30% to 50%) in the organic amendments was rendered available over the five week duration of the experiments. Amendments that had higher N content and lower carbon (C):N ratios such as dried distillers grain with solubles resulted in greater canola N uptake. Reduced germination and emergence of canola seedlings was observed at high rates of addition of distillers grain (400 kg N ha^?1), the reason for which is unclear but may be due to a localized salt or toxicity effect of the amendment. The amendment with alfalfa powders and distiller grains resulted in small increases in residual soil nutrients. Effects on pH, salinity, organic carbon and extractable metals tended to be small and often not significant. Alfalfa powders and distillers grains appear to be quite effective in supplying nutrients, especially N, for plant growth over the short-term.     

RESPONSE OF MAIZE GENOTYPES TO SEVERAL MYCORRHIZAL INOCULUMS IN TERMS OF PLANT GROWTH,NUTRIENT UPTAKE AND SPORE PRODUCTION

A greenhouse experiment was conducted at the University of Çukurova, Rhisosphere Lab, Adana, Turkey, on a growth medium to assess the impact of several selected mycorrhiza including indigenous AMF-maize hybrid combinations on spore production, plant growth and nutrient uptake. In the experiment, six maize (Zea mays L.) (Luce, Vero, Darva, Pegasso, P.3394, and P.32K61) genotypes were used. Control, Glomus mossea, G. caledonium, G. etunicatum, G. clarium, G. macrocarpum, G. fasciculatum, G. intraradices, Dr. Kinkon (Japanese species), indigenous mycorrhizae (Balcal? series) and cocktail mycorrhizae species spores were used. The growth of maize genotypes was found to depend on the mycorrhizal species. For shoot and root dry weight production G. intraradices is one of the most efficient mycorrhiza species on average on all maize genotypes. Genotypes P.3394 and P.32K61 produced the highest shoot and root dry weight as well. Pagasso and Darva genotypes compared to the other genotypes have high root colonization percentages. On average G. clarium inoculated plants also have high percentages of root colonization. It has been found that the P.32K61 genotype has a high phosphorus (P)% content and Pagasso genotypes have higher zinc (Zn) content uptake than other genotypes. G. clarium inoculated maize genotype plant tissues have high P% and Zn content. G. intraradices is also efficient for P and Zn uptake. Mycorrhizal dependent maize genotypes showed variability in P efficiency from inefficient to efficient genotypes.     

INTERACTION OF MOLYBDENUM AND PHOSPHORUS SUPPLY ON UPTAKE AND TRANSLOCATION OF PHOSPHORUS AND MOLYBDENUM BY BRASSICA NAPUS

A hydroponic trial was conducted to assess interaction of molybdenum (Mo) and phosphorus (P) on uptake and translocation of P and Mo by Brassica napus. Molybdenum was applied at four rates (0, 0.01, 0.1 and 1 mg L^?1) and P at three rates (1, 30, and 90 mg L^?1) in nutrient solution. The results indicated that P increased shoot growth and 0.01 mg L^?1 Mo improved the growth of shoots and roots. Molybdenum increased shoot P uptake and root P concentration and uptake when higher P was provided, and had a stimulating effect on P translocation from shoots to roots. P increased shoot Mo concentration and uptake, decreased those in roots, and enhanced Mo transport from roots to shoots. These results implied that both Mo and P had beneficial effects on Mo and P absorption and translocation and co-application of them were necessary to promote growth and utilization of Mo and P for Brassica napus.     

低盐基土壤K、Ca、Mg的交互作用对水稻生长与养分吸收的影响

本文研究了我国南方的低盐基土壤施用Ｋ、Ｃａ、Ｍｇ对水稻生长,养分迁移和养分吸收的影响,结果表明,在低盐基土壤ＮＰ基础上施Ｋ,随Ｋ用量增加到一定量,水稻生长受阻,生长量和产量下降的趋势。ＮＰＫ肥基础上施Ｍｇ生长量随之增加;ＮＰＫ肥基础上Ｃａ,Ｍｇ一起补给,产生协同作用,使水稻生长量明显增加。阳离子交换树脂模拟试验表明,Ｋ－Ｍｇ、Ｋ－Ｃａ和Ｍｇ－Ｃａ在养分向根迁移过程中即存在正的交互作用。盆载试验表明     

大豆品种耐低磷和对磷肥效应的遗传差异

大豆不同品种对低磷胁迫和磷肥效应有显著的遗传差异。低磷胁迫下,苗期植株叶片酸性磷酸酶活性（APA）和叶面积与品种的子粒产量呈极显著相关;APA、叶面积、干物质量和氮积累量与生物学产量呈显著或极显著相关。但在施磷条件下,它们之间则无相关关系。这表明APA、叶面积、干物质量和氨积累量可作为耐低磷基因型的筛选指标。试验表明,耐低磷能力弱的品种对磷肥反应更为敏感。在低磷和施磷条件下产量均较高的品种也是存在的,它们对不同的磷素营养环境有着广泛的适应性,进一步挖掘这类基因型很有意义。     

INTERACTION EFFECT OF POTASSIUM AND SULFUR FERTILIZATION ON PRODUCTIVITY AND MINERAL NUTRITION OF SUNNHEMP

The interactive effect of potassium (K) and sulfur (S) fertilization on productivity and mineral nutrition of sunnhemp (Crotalaria juncea L.) was evaluated in a field experiment during 2008 and 2009 cropping seasons at Uttar Pradesh, India. Potassium and sulfur fertilizers increased fiber yield and nutrient uptake of sunnhemp. It was observed that an application of K and S at 40 kg ha^?1 each significantly increased the total dry matter, fiber yield, and nutrient uptake of sunnhemp. The crop yield response to the added S was greater than for K and the nutrient use efficiency was also higher at lower levels of fertilizer addition. The increased levels of K and S improved the number of nodules and crude protein content of sunnhemp leaves.     

菌根真菌对茶树吸收磷、钾素的影响

以辐照灭菌的酸性黄壤为供试土，种植有性繁殖的茶苗并接种ＶＡ菌根真菌，以不接种菌根真菌为对照，分别追施３２Ｐ－过磷酸钙和＾８６Ｒｂ－氯化铷，试验结果表明：菌根侵染率，＾３２Ｐ组合合为５２．６０％，＾８６Ｒｂ组合为５６．７０％，茶树株高分别对照的２．１０倍和２．５０倍，对磷、钾元素的吸收量分别比对照增加４．６０和３．１０倍，磷肥利用率提高１４．１０％，钾肥利用率提高１７．１３％。     

EXPLOITATION OF PHOSPHORUS PATCHES WITH DIFFERENT PHOSPHORUS ENRICHMENT BY THREE ARBUSCULAR MYCORRHIZAL FUNGI

The effect of three arbuscular mycorrhizal (AM) fungi on phosphorus (P) nutrient activation and acquisition by maize from spatially heterogeneous sand was investigated using dual-mesh packages enriched with different P concentrations and compared with non-mycorrhizal cotrols. As would be expected the AM fungi significantly enhanced leaf photosynthetic rate and the biomass and P concentrations in shoots and roots. All three fungi (Glomus intraradices, Glomus mosseae and Glomus etunicatum) displayed the capacity to dissolve inorganic P and promoted P nutrient availability in the packages (P patches). G. etunicatum showed the largest effect comparing with Glomus intraradices and Glomus mosseae, particularly in packages with high concentrations of P. Possible mechanisms involved include the acidification of the P patches by the AM fungi, promotion of the dissolution of the P, and more marked effects of the three fungal isolates with increasing enrichment of P in the patches. Inoculation with G. etunicatum resulted in greater acidification compared to the other two fungi. We conclude that AM fungi can promote P availability by acidifying the soil and consequently exploiting the P in nutrient patches and by facilitating the growth and development of the host plants.     

水分胁迫及VA菌根接种对绿豆生长的影响

本文采用土培试验研究了水分胁迫下接种VA菌根真菌Glomusmosseae,G .sp .和G .caledonium对绿豆生长及代谢活动的影响。结果表明 ,水分胁迫严重抑制了植株的生长 ,但对VA菌根真菌的侵染能力影响不大。接种VA菌根真菌不仅有利于植株对土壤中磷和氮的吸收 ,而且明显改善了植株的水分状况 ,降低了植株叶片的脯氨酸含量 ,提高了接种株叶片的光合效率 ,显著增加了植株干物质量 ,增强了绿豆的抗旱性或耐旱性。 3种真菌中 ,以Glomusmosseae的接种效果最好。     

玉米、小麦细胞磷、锌营养及交互作用的研究

采用溶液培养研究玉米、小麦在不同磷、锌浓度下细胞磷、锌营养及交互作用。结果表明,随着外界磷浓度（０,０．１２,０．６,３．０ｍｍｏｌ／Ｌ）的提高,玉米、小麦根系和叶片细胞壁、细胞质和液泡中磷含量也随之增高,但细胞壁和液泡中磷的含量高于细胞质中的含量。介质中磷浓度的提高使根细胞壁中锌的含量增加,但高磷（３．０ｍｍｏｌ／Ｌ）处理使细胞质、液泡中锌的含量下降。高磷处理抑制了两种作物的锌由根系向地上部的转运,同时叶片中大部分锌被结合在细胞壁中,因而叶细胞中的细胞质和液泡处于相对的低锌状态。与足量锌（２．０ｍｏｌ／Ｌ）供应比较,低锌（０．１ｍｏｌ／Ｌ）处理使玉米、小麦根系和叶细胞壁、细胞质和液泡中磷的浓度增高。低锌和过量磷的供给抑制了植株的生长,最终使干物质的积累下降。     

INTERACTIVE EFFECTS OF SALINITY AND PHOSPHORUS NUTRITION ON PHYSIOLOGICAL RESPONSES OF TWO BARLEY SPECIES

Salinity is one of the most important agricultural problems in Iran. The effect of different levels of salinity and phosphorus on shoot length, root and shoot fresh and dry weight, nutrient elements (sodium (Na⁺), potassium (K⁺), phosphorus (P) and chloride (Cl^?), proline and soluble sugar contents of barley were investigated. Two cultivars of barley, Hordeum murinum (wild resistant germplasm) and Hordeum vulgar, variety Afzal were treated in vegetative stage under hydroponics condition in a factorial arrangement based on completely randomized block (CRB) design with four levels of salinity [0, 100, 200 and 300 mM sodium chloride (NaCl)] and three levels of phosphorus (15, 30 and 55 μm L^?1) with three replications. By increasing salinity, all the measured parameters, except sodium (Na⁺) content were reduced. Furthermore, with increased in phosphorus levels from 15 to 55 μm, Na⁺ content of the plant shoots decreased, but length, fresh and dry weights of roots and shoots and K⁺, P, Cl^?, proline, and soluble sugars content of the shoots increased. The results indicated that accumulation of mineral ions for osmotic adjustment and restriction of Na⁺ accumulation in shoots were involved in phosphorus enhancement of the salt tolerance of barley. Thus, it seems that in saline soils, where there is no possibility for soil leaching and amending, application of phosphorus fertilizers can lead to a satisfactory growth and production in barely yield.