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1.
Studies have reported significant differences in nitrate accumulation among genotypes within a crop species, but the reason for these differences is not clear. This study investigated nitrate (NO3) efflux from roots of two oilseed rape cultivars (Brassica napus L. cvs. ZY821 and D89) and the relationship between nitrate efflux and plant nitrate accumulation. Nitrate efflux was estimated by measuring nitrate released from roots into nitrate-free nutrient solutions 58 days after sowing. The solutions were buffered with either 2.0 mM (n-morpholino) ethanesulfonic acid (MES) or 0.05 mM phosphate. Whole-plant and petiole nitrate accumulations were significantly greater for ZY821 compared to D89. Nitrate efflux varied diurnally, and the difference between cultivars was greater in the morning than in the afternoon. Data suggested that the relatively high pH of the phosphate-buffered solutions increased nitrate efflux rates from oilseed rape plants; therefore phosphate buffer should not be used when making direct determinations of nitrate efflux. Maximum cumulative nitrate efflux was 0.97 μmol g?1 fresh-weight (FW) root for ZY821 and 1.9 μmol g?1 FW root for D89. Maximum nitrate efflux rate was 0.084 μmol g?1 FW min?1 for ZY821 and 0.097 μmol g?1 FW min?1 for D89. These results indicated that between the two cultivars, ZY821 had the greatest petiole nitrate nitrogen concentration and the lowest total nitrate efflux. We suggest that cultivars with low nitrate efflux rates are able to translocate and store greater amounts of nitrate in aboveground plant organs, especially in petioles.  相似文献   

2.
Evaluations of vegetative growth and leaf concentrations of nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg), and iron (Fe) were made of apple (Malus domestica Borkh. cvs. Granny Smith, Gala, and Golab) grown with five treatments of NO3?:NH4+ ratios in pot culture. The concentrations of NO3?:NH4+ ratios were 2.5:0.1, 6:0.3, 6:0.5, 6:0.7, and 6: 1 meq L?1. Regression analysis showed that growth parameters of main stems and branches were not affected by increases of NH4+ in the ratios. Granny Smith, Gala, and Golab differed in some of these parameters. Concentrations of N and Fe increased as NH4+ increased, whereas K and Ca decreased and Mg was not affected significantly. Generally, the treatment of 2.5:0.1 produced leaves with lower N but higher K, Ca, and Mg concentrations than the other treatments. This research showed that vegetative growth was not affected by NH4+ concentration whereas elemental composition was affected.  相似文献   

3.
ABSTRACT

A pot experiment was conducted to study the growth and pungency of Allium cepa L. grown in Perlite as affected by colonization by the arbuscular mycorrhizal (AM) fungi Glomus versiforme and Glomus intraradices BEG141 and by ammonium:nitrate (NH4 +:NO3 ?) ratios of 3:1, 1:1, and 1:3 in 4 mM solutions. Plants were harvested when bulb formation commenced. In general, mycorrhizal colonization resulted in increased shoot dry weight, shoot length, sheath diameter, root nitrogen (N) and phosphorus (P) content (except with G. intraradices and a NH4 +:NO3 ? ratio of 1:3), shoot N and P concentrations (except with G. versiforme and a NH4 +:NO3= ratio of 3:1) and content. Plants inoculated with G. versiforme had higher growth parameters and N and P content than those with G. intraradices, whereas N and P concentrations showed the opposite trends. Growth parameters and N and P content of non-mycorrhizal plants were highest at a NH4 +:NO3= ratio of 1:3, while those of plants inoculated with G. versiforme or G. intraradices were highest at a ratio of NH4 +:NO3 ? 3:1 or 1:1. Neither mycorrhizal colonization nor proportion of inorganic N species significantly affected bulb enzyme-produced pyruvate or total or organic sulfur (S) concentrations in plant shoots. Colonization by AM fungi made a substantial contribution to onion growth and may not have been directly related to bulb pungency at early stages of plant growth. However, the influence of AM fungi on plant N and P metabolism may have implications for onion flavor at later stages of plant growth.  相似文献   

4.
王伟  宋文峰  赵学强  沈仁芳 《土壤》2015,47(5):853-857
相对于NO_3~--N,NH_4~+-N可以缓解植物铝毒害,但其机制还不清楚。铝在细胞膜表面的吸附量受细胞膜表面电势影响,直接影响到铝毒害。本文采用酶解法提取水稻根尖原生质体,研究了原生质膜表面zeta(ξ)电势对NH_4~+N、NO_3~--N、铝和pH的响应。结果表明,不管是否加铝,NH_4~+-N和NO_3~--N本身并不显著影响原生质膜表面ξ电势,但是加铝和降低pH均可以去极化原生质膜表面ξ电势。水稻吸收NH_4~+-N一般降低生长介质pH,吸收NO_3~--N升高生长介质pH。因此,NH_4~+-N缓解水稻铝毒的原因,不是因为NH_4~+-N本身去极化了细胞膜表面电势,而是因为水稻吸收NH_4~+-N降低了介质pH,去极化了细胞膜表面电势,从而可能降低了铝在水稻根尖表面的吸附。  相似文献   

5.
[目的]探究百里杜鹃景区马缨杜鹃林下凋落物覆盖对土壤蒸发的影响,为其林下水源涵养功能的发挥提供科学依据。[方法]根据土壤蒸发理论,采用自制微型蒸渗装置进行模拟试验,参照百里杜鹃林下凋落物现存状况,研究不同质量相同比例及相同质量不同比例的凋落物覆盖对土壤蒸发抑制的影响。[结果]不同分解程度凋落覆盖比例相同质量不同时,土壤水分蒸发量随凋落物的质量增大而减小;土壤水分蒸发量随着土壤含水率的下降呈递减关系,当含水率每下降0.5%时,60,30和15 g凋落物覆盖的土壤水分蒸发量分别依次减少了0.11,0.49和1.23 g。凋落物覆盖质量相同时,不同分解程度的凋落物对土壤水分蒸发抑制作用不同,表现为:未分解半分解已分解。[结论]凋落物覆盖量越多,对土壤蒸发的抑制作用越大;凋落物分解程度越低,对土壤蒸发的抑制作用越好。  相似文献   

6.
李海英  彭光浩 《土壤》2007,39(6):896-899
以生菜和青菜作为供试材料,在液培条件下,研究了不同形态N素对蔬菜硝酸盐累积的影响,同时观测了叶面喷施两种低硝酸化剂的化控技术对降低蔬菜硝酸盐积累的效果.研究结果表明:N素形态对生菜的生长和硝酸盐的积累影响显著,而对青菜的影响不显著;相同形态N素供应时,青菜叶片中硝酸盐含量显著高于生菜中的含量,硝酸盐的积累取决于不同蔬菜品种的遗传特性.青菜和生菜叶面喷施两种低硝酸化剂对降低蔬菜中硝酸盐含量的作用不显著.  相似文献   

7.
ABSTRACT

The purpose of this work was to determine the effects of pre-plant micronutrient mixes and various ammonium/nitrate (NH4 +/NO3 ?) ratios in a fertilizer solution on growth and the nutrient uptake of French marigold ‘Orange Boy’ in a plug culture. Two kinds of granular glass frits containing six micronutrients were produced and incorporated into peatmoss+vermiculite (1:1, v/v) substrate at a rate of 0.3 g · L? 1. The five NH4 +/NO3 ? ratios in fertilizer solution were 0/100, 25/75, 50/50, 75/25, and 100/0, each giving a total nitrogen (N) supply of 80, 100, and 120 mg · L? 1in the root substrate during stages 2, 3, and 4, respectively. The plants produced a significantly higher fresh and dry matter yield at 35 days after sowing when grown in 25/75 N nutrition in micronutrient fertilizer 1 (MF1) and 50/50 in micronutrient fertilizer 2 (MF2) than in the other NH4 +/NO3 ? ratios tested. Treatments of the highest accumulation of iron (Fe), manganese (Mn), zinc (Zn), copper (Cu), and boron (B) in plants were 25/75, 50/50, 50/50, 25/75 and 50/50 in MF1 and 50/50, 25/75, 50/50, 50/50, 50/50 in MF2, respectively. The trends in accumulations of Fe, Mn, Zn, Cu, and B in plants were quadratic (Q), linear (L) and Q, L and Q, L and Q, and Q, respectively, in MF1. Those in MF2 were Q, Q, Q, L and Q, and Q, respectively.  相似文献   

8.
ABSTRACT

Two cultivars of pea: ‘PS210713’ (‘PS’), sensitive to iron deficiency, and ‘Marveille de Kelvedon’ (‘MK’), tolerant, were cultivated in controlled climatic conditions during one month, on a nutrient solution containing either nitrate (NO3 ?, 4 mM) or ammonium (NH4 +, 4 mM) and in the presence of bicarbonate (10 mM). The effects of these nitrogen forms on the growth and the mineral nutrition, and especially iron nutrition are analyzed.

The reduction of growth by bicarbonate was approximately 30% in case of NO3 ? nutrition in the two cultivars, whereas in ammoniacal treatment the reduction is only 6% and 18% respectively in ‘PS’ and ‘MK’ cultivars.

In presence of bicarbonate, the plant growth is not stimulated by NO3 ?relatively to its growth on ammoniacal medium, as often noticed when plants are cultivated on medium without bicarbonate: In presence of this compound, the biomass production of plant pea, was not influenced by the nitrogen forms. The nitric source led to a ferric chlorosis in the sensitive cultivar plants whereas any chlorosis was noted when ammoniacal source was applied. On the other hand, nitric nitrogen form decreased the nitrogen feeding of plants and increased the potassium one, while the effect of the ammoniacal nitrogen form on these nutrients was quite the inverse. In addition, the later increased the allocation of iron towards shoots. Besides, with this nitrogen source there was not accumulation of nitrate in the plant tissues. In nitric feeding case, the nitrate is mainly accumulated in the roots of the two cultivars. It is noticeable that the sensitive cultivar (‘PS’) accumulates three times more nitrate than the tolerant one (‘MK’).

On the level of the whole plant, the iron and phosphorus nutrition seems unaltered by the nitrogen form.  相似文献   

9.
《Journal of plant nutrition》2013,36(8):1683-1696
Abstract

Soil acidity is one of the limiting factors affecting the production and sustainability of pastures and crops in many parts of the world. An on‐farm experiment was conducted in Australia to investigate the cultivar variation in alfalfa (lucerne) (Medicago sativa L.) with respect to soil acidity and response to applied lime. The experimental site was a brown sandy clay loam with a soil pH of 4.8 (1:5 calcium chloride). Ten cultivars (Hunter River, Hunterfield, Sceptre, Aurora, Genesis, Aquarius, Venus, PL90, PL55, and breeding line Y8804) were tested at two levels of lime (0 and 2 t ha?1). Lime application significantly increased the root growth, nodulation, leaf retention, leaf to stem ratio, herbage yield, and crude protein content of alfalfa. Liming had a significant effect on elemental composition of alfalfa shoots. Aluminum (Al) concentration was reduced from 93 mg kg?1 DM in nil lime treatment to 45 mg kg?1 DM in +lime treatment. Similarly, manganese (Mn) and iron (Fe) shoot concentrations were reduced from 74 mg kg?1 DM and 92 mg kg?1 DM to 59 mg kg?1 DM and 76 mg kg?1 DM, respectively. Liming significantly improved the calcium (Ca) concentration of shoots, while there was a little effect on phosphorus (P) and zinc (Zn) concentrations of alfalfa shoots. Cultivars had differential response to lime application. Response to lime application was greater in Y8804 and Aurora alfalfa where yield increased by 32% and 31%, while yield increase was 11–22% in other cultivars. Cultivars also differed significantly in root growth, nodulation, leaf drop, leaf to stem ratio, crude protein content, and elemental composition of shoots. Cultivars with better performance in no liming treatment had comparatively lower shoot Al, Mn, and Fe concentrations compared with other cultivars.  相似文献   

10.
本试验研究了农用化肥代替化学试剂,做无土栽培营养液肥源的可行性。试验结果表明,利用普通化肥代替化学试剂配制营养液,不仅是可行的,而且能大幅度降低成本,经济效益显著。在春夏季试验中,适当增加营养液中氨态氮化肥的比例,明显地促进黄瓜早熟,提高早期产量。在秋冬季试验中,适当比例的氨态氮肥,能促进黄瓜总产量的提高。春秋两次试验还表明,若含硝态和酰胺态氮的化肥同时作氮源时,可不同程度地促进黄瓜的营养生长及生殖生长。此外本试验还研究了不同营养液对黄瓜叶片中叶绿素的含量,氨基酸的含量及光合作用的影响。  相似文献   

11.
环境因素对蔬菜累积硝酸盐影响的研究进展   总被引:18,自引:3,他引:18  
蔬菜中的硝酸盐是一种对人体有害的化学物质,其含量比其它植物都高,人体摄入它会引起多种疾病。对近年来国内外有关光照、温度、气体、土壤中的水分和养分等对蔬菜硝酸盐积累的影响进行了综述。  相似文献   

12.
ABSTRACT

Interactions between nitrate (NO3 ?), potassium (K+), and ammonium (NH4 +) were investigated using hydroponically grown cucumber (Cucumis sativus L.) plants. Ammonium as the sole nitrogen (N) source at 10 mM was toxic and led to overall growth suppression, chlorosis, and necrosis of leaves. After 20 days, 50% of the plants were dead. However, when NO3 ? was supplied at very low concentration together with high NH4 + (only 1% of total 10 mM N) all seedlings survived and their growth was improved. High K+ concentration (5 mM) also alleviated NH4 + toxicity and increased plant growth several fold compared to intermediate concentration of K+ (0.6 mM). Leaf total N and 15N derived from 15N-labelled NH4 + increased in the presence of NO3 ?, but decreased at high K+ concentration. High K+ supply enhanced total carbon (C) and δ 13C and stimulated GS and PEPCase activities in leaves and roots. Nitrate supplementation had no effect on GS or PEPCase activities. It is concluded that K+ may alleviate NH4 + toxicity, partly by inhibiting NH4 + uptake, partly by stimulating C and N assimilation in the roots.  相似文献   

13.
ABSTRACT

The source of nitrogen (N) used in soil fertility practices affects plant growth, nutrient absorption, and the availability of nutrients. Consequently, the potential of plants to extract zinc (Zn) from soils may be increased by controlling the ratio of NH4 + to NO3 ? to maximize growth and Zn accumulation. The objectives of this research were to determine the effects of Zn supply and different molar ratios of NH4 + to NO3 ? on growth and Zn accumulation in Indian mustard (Brassica juncea Czern.). In a factorial experiment with solution culture, Indian mustard (accession 182921) was supplied with two concentrations of Zn (0.05 and 4.0 mg L?1) in combination with six N treatments with different molar percentage ratios of NH4 + to NO3 ? (0:100, 10:90, 20:80, 30:70, 40:60, and 50:50) for three weeks. Zinc supplied at 0.05 mg Zn L?1 represented a common concentration of Zn in solution culture, whereas 4.0 mg Zn L?1 was excessive for plant nutrition. If the supply of Zn in solution was excessive, plants developed symptoms of foliar chlorosis, which became severe if plants were supplied with 80% of N as NO3 ?. Supplying high proportions of NO3 ? in the nutrient medium stimulated Zn accumulation, whereas increasing proportions of NH4 + (up to 50% of the total N) enhanced shoot growth. The pH of nutrient solutions generally decreased with increasing proportion of NH4 + in solutions and with increased Zn supply. The Zn phytoextraction potential of Indian mustard was maximized, at about 15 mg Zn plant?1, if plants received 10% of the total N as NH4 + and 90% as NO3 ?.  相似文献   

14.
Strawberry (Fragaria ananasa) plants were grown in hydroponics in a greenhouse, where the supply of different ammonium (NH4+): nitrate (NO3?) ratios, was investigated to reduce the negative effect of alkalinity in nutrient solutions. The experiment was arranged in factorial combination with two factors, NH4+:NO3? ratios (0%:100%, 25%:75%, 50%:50%, 75%:25%, and 100%:0%) and bicarbonate (0, 25, 50, and 75 mM; NaHCO3). Plants treated with increasing bicarbonate concentrations exhibited significantly inhibited growth. In plants treated with NH4+ and NO3? simultaneously, there was a counteraction of the bicarbonate-induced growth suppression. Sole NO3? application in the presence of high bicarbonate resulted in poor growth and plant death due to high alkalinity. The adverse effect of alkalinity on SPAD values and maximal quantum yield of PSII photochemistry (Fv/Fm) alleviated with increasing proportion of NH4+ in nutrient solutions. The results showed that fruit numbers and yield of strawberry increased with elevation of NH4+ in nutrient solutions.  相似文献   

15.
《Journal of plant nutrition》2013,36(12):2413-2424
Abstract

Tomato and watermelon plants were grown in nutrient solutions in which nitrogen (N) was supplied as NO3 ? (6 mM‐N) or NH4 + (6 mM‐N). The experiments were conducted to evaluate the effect which different N sources exert on iron (Fe) uptake and accumulation, on the enzymatic activities of aconitase (Aco), chelate reductase (FeCH‐R), peroxidase (POD), catalase (CAT), and Fe‐superoxide dismutase (FeSOD), and on biomass production. For both species of plants, fertilization with NH4 + caused the total Fe concentration to be lower, in the roots and in the leaves in relation to the concentrations recorded in plants fertilized with NO3 ?. The response of the enzymes related to Fe correlated with their concentration. The plants treated with N?NO3 ? registered the highest activities in Aco, FeCH‐R, POD, and CAT for both tomato and watermelon. On the other hand, only in the tomato plants was the superoxide dismutase (SOD) activity appreciably influenced primarily by NH4 +, due possibly to the toxic effect of this N source. Finally, in relation to biomass production, fertilization with NH4 + drastically reduced growth in the tomato plants, while in watermelon plants, no significant alteration was detected in dry‐matter production, regardless of the N form used. It was concluded that the response of the parameters analyzed to NH4 + fertilization, in tomato and watermelon, compared to fertilization with NO3 ? was similar. By contrast, tomato plants, but not watermelon plants, were negatively influenced by NH4 +.  相似文献   

16.
Nitrogen is taken up by most plant species in the form of nitrate and ammonium. The objective of this study was to investigate the effect of different nitrogen forms on the growth of watermelon seedlings. Plants were grown in hydroponic culture with five nitrate (NO3?)/ammonium (NH4+) ratios (100/0, 75/25, 50/50, 25/75, 0/100). When the proportion of NH4+ was increased, the leaf number, leaf area, shoot height, net photosynthesis, biomass, and root growth were significantly decreased. Higher concentrations of nitrogen (N) and phosphorus (P) were observed when plants were supplied with mixed NO3? and NH4+ compared to NO3? or NH4+ alone, whereas the concentrations of potassium (K), calcium (Ca), and magnesium (Mg) were decreased with increasing NH4+. The microelements concentrations were generally increased with more NH4+ added. In addition, plants fed with higher NO3?/NH4+ ratios resulted in more minerals accumulation.  相似文献   

17.
ABSTRACT

Two separate field trials comparing three different commercial sulfur (S) fertilizers were conducted in 2016 and 2017, respectively, to evaluate their initial agronomic effectiveness in increasing soybean grain yield. The S compounds of these products were elemental S (ES) and ammonium sulfate (AS). The fertilizer sources were (1) AS, (2) granular MAP-10 S (5% ES + 5% AS-S) and (3) bulk-blend (bentonite-ES) + (AS) containing 25% ES + 25% AS-S. Rates of total S applied were 0, 5.6, 11.2, 22.4 and 33.6 kg S ha?1. A significant S response in grain yield was observed with all S sources. The grain yield was significantly higher with AS than with the other two S sources at S rates ≤22.4 kg S ha?1 of total S applied but equal for all sources above this rate. All data points for the three S sources followed the same S response function based on a quadratic plateau model when the grain yield was plotted versus AS-S rate applied. This suggests that there was no significant ES oxidation from MAP-10S and (ES) + (AS) products to provide soybean crop growth to maturity. The experimentally observed AS-S rate at which the maximum grain yield was attained in the 2 years of fieldwork was 11.2 kg S ha?1 while the calculated average AS-S rate based on a quadratic plateau model was 13.4 kg S ha?1.

Abbreviations: AS: ammonium sulfate; ES: elemental S; MAP: monoammonium phosphate; DAP: diammonium phosphate; TSP: triple superphosphate.  相似文献   

18.
The effect of ammonium:nitrate (NH4:NO3) ratio in nutrient solution on growth, photosynthesis (Pn), yield, and fruit quality attributes in hydroponically grown strawberry (Fragaria × ananassa Duch) cvs. ‘Camarosa’ and ‘Selva’ was evaluated. There were four nutrient solutions of differing NH4:NO3 ratios as follows: 0:100, 25:75, 50:50, and 75:25. Plants grown in solution with 75% NH4 had lower leaf fresh and dry weights and leaf area than those with 25% NH4 in both cultivars. High ratios of NH4 and NO3 in the solution always reduced the yield. The yield was increased by 38% and 84% in ‘Camarosa’ and ‘Selva,’ respectively, when the plants were grown in the 25NH4:75NO3 solution compared with plants grown in a higher NH4 ratio solution. The increased yield at the 25 NH4:75NO3 ratio was the result of the increase in fruit size, i.e., length and fresh weight of fruits. Plants grown in the 25NH4:75NO3 solution had the highest rate of Pn, while those grown in 75NH4:25NO3 solution had the lowest Pn rates in both cultivars. Increasing the NH4 ratio in the solution from 0 to 75% significantly reduced the calcium (Ca) concentration and postharvest life of the fruits in both cultivars. Both higher leaf area and Pn rate appeared to be the reason for the increased yield and plant growth in the 25:75 ratios of nitrogen (N). The results indicate the preference of strawberry plant growth toward a greater nitrate N form in a hydroponic solution. Therefore, a combination of two forms of N in an appropriate ratio (25NH4:75NO3) appears to be beneficial to plant growth, yield, and quality of strawberry fruits.  相似文献   

19.
低浓度NaCl对水培韭菜生长、产量及品质的影响   总被引:4,自引:0,他引:4  
NaCl一直以来被认为是一个胁迫因子,且是造成植物盐害的主要因素,而低浓度NaCl对植物生长不仅没有危害,反而具有促进植物生长,提高品质的作用。韭菜营养液水培作为一种新型的栽培方式,很好的解决了韭蛆等病虫的为害。为了探究低浓度NaCl添加到营养液中对韭菜生长、品质和产量的影响,试验设定了0 mmol·L-1(对照)、5 mmol·L-1、10 mmol·L-1、20 mmol·L-1、30 mmol·L-1 5个NaCl浓度,研究不同浓度NaCl处理下韭菜生长、生理和产量的变化。研究结果表明,韭菜的株高、假茎粗和叶长均以20 mmol·L-1 NaCl处理下最高,各处理的叶片数均多于对照,叶片宽度随着NaCl浓度的增加呈现下降趋势。低浓度NaCl增加了韭菜的光合色素含量,10 mmol·L-1处理下叶绿素a、叶绿素b、类胡萝卜素和叶绿素a+b含量最高。NaCl处理能有效降低韭菜中硝酸盐的积累,各NaCl处理硝酸盐含量分别比对照下降12.42%、13.17%、15.16%和26.76%。Vc含量随添加NaCl浓度的增加表现出先上升后下降的趋势,其中各NaCl处理含量分别比对照高12.2%、12.8%、5.1%、-15.4%,可溶性糖与粗纤维均在10 mmol·L-1时达到最大值。产量以10 mmol·L-1处理最高,3茬产量及总产量分别比对照处理增加6.7%、6.6%、6.9%、6.7%。综合分析认为,韭菜营养液中添加10 mmol·L-1的NaCl,在促进韭菜生长,降低硝酸盐,改善品质和提高产量上综合表现最佳。  相似文献   

20.
铵钾施用次序和比例对油菜生长和氮钾养分吸收的影响   总被引:5,自引:3,他引:2  
陈小琴 《土壤》2008,40(4):571-574
通过油菜盆栽试验研究了铵(NH4 )钾(K )离子施用次序和比例对这两种离子在土壤中的交互作用及其对油菜生长和N、K养分吸收的影响.结果表明:当NH4 、K 摩尔比为1:1时,先NH4 后K ,油菜的生物产量和N、K养分吸收量均较低,该结果与后施入的K 促进了先施入的NIL4 的固定,降低了土壤NH4 -N含量有关.而当NH4 、K 质量比为1:1(其摩尔比约为3:1)时,施用次序对该土壤油菜的生长和养分吸收影响不大,这也说明了合适的N、K比例对作物生长的重要性.  相似文献   

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