饲料蛋白水平对星斑川鲽幼鱼生长、体组成及血浆生化指标的影响

以鱼粉为蛋白源,配制5个不同蛋白质水平(34.85%,40.48%,46.54%,51.54%,56.69%)的等能饲料.以初始体质量为(54.52±0.23)g的星斑川鲽(Platichthys stellatus)为实验对象,在室内循环水养殖系统中进行54 d的摄食生长实验,研究饲料蛋白水平对星斑川鲽幼鱼生长、体组成及血浆生化指标的影响.结果显示:(1)增重率(WGR)和特定生长率(SGR)随着饲料蛋白水平的增加而上升,51.54%和56.69%饲料组之间差异不显著(P>0.05),其余各组差异显著(P<0.05);51.54%饲料组的蛋白质效率(PER)显著高于其他各组(P<0.05);51.54%饲料组的蛋白质沉积率(PRE)也显著高于34.85%、46.54%和56.69%组(P<0.05),但与40.48%饲料组差异不显著(P>0.05).以增重率为参考指标,折线回归分析结果表明,星斑川鲽幼鱼获得最佳增重时对饲料中蛋白质的需要量为53.56%.(2)饲料不同蛋白水平对星斑川鲽幼鱼鱼体灰分含量没有显著影响,但显著影响了鱼体粗蛋白、粗脂肪和水分的含量(P<0.05).51.54%饲料组鱼体粗蛋白含量最高,显著高于34.85%组(P<0.05),而与40.48%、46.54%和56.69%组之间无显著差异(P>0.05);鱼体粗脂肪含量随着饲料蛋白水平的升高而下降,水分含量表现出与粗脂肪含量相反的趋势.(3)饲料蛋白含量对星斑川鲽幼鱼部分血浆生化指标也产生了显著性的影响.血浆总蛋白(TP)以51.54%组最高,与56.69%组差异不显著(P>0.05),,但显著高于34.85%、40.48%和46.54%组(P<0.05);40.48%、51.54%和56.69%组间血浆尿素氮(BUN)含量差异不显著(P>0.05),但显著低于34.85%和46.54%组(P<0.05).综合以上结果,星斑川鲽饲料中蛋白质适宜添加量为51.54%～53.56%.     

饥饿后再投喂对星斑川鲽生化组成以及能值的影响

采用饥饿不同时间后再恢复投喂相同时间的方法,在温度19±1℃、盐度32±1的条件下,对相同规格的星斑川鲽(26.02±0.30 g)鱼体和粪便的生化组成以及能值等相关指标的影响进行了研究.研究结果表明,饥饿后再投喂对星斑川鲽鱼体内水分、脂肪和灰分的含量影响显著,对鱼体内蛋白质含量无显著影响;对粪便脂肪和蛋白质含量影响显著,对粪便中灰分无显著影响;对鱼体能值影响显著,对粪便的能值无显著影响.研究还发现,饥饿处理时间越长,各相关指标变化越显著;随着恢复投喂的进行各相关指标逐渐恢复到对照组水平,饥饿处理时间越长,恢复到对照组水平所需要的时间越长.     

紫花苜蓿浓缩叶蛋白替代鱼粉对星斑川鲽幼鱼生长、体组成及血液生化指标的影响

研究了紫花苜蓿浓缩叶蛋白替代0%(S0)、10%(S10)、20%(S20)、30%(S30)、40%(S40)、50%(S50)的鱼粉蛋白对星斑川鲽(Platichthys stellatus)幼鱼生长、体组成及血液生化指标的影响。实验幼鱼体质量(83.0±0.20)g。结果显示:1)当替代比例≤20%时,各组间星斑川鲽幼鱼特定生长率(SGR)、日摄食率(DFI)、饲料效率(FE)和蛋白质效率(PER)均无显著变化,高于此值时呈显著下降(P<0.05)。随饲料中紫花苜蓿浓缩叶蛋白替代鱼粉比例的升高,鱼体肥满度(CF)呈显著下降趋势(P<0.05),而消化道指数(DTI)则呈显著上升趋势(P<0.05);各实验组干物质、蛋白质及脂肪表观消化率均呈直线下降趋势(P<0.05)。以星斑川鲽幼鱼特定生长率(SGR)为参考指标,采用折线回归分析得出,在本实验条件下,星斑川鲽幼鱼获得最佳生长时紫花苜蓿浓缩叶蛋白替代鱼粉蛋白的适宜比例为19.0%。2)紫花苜蓿浓缩叶蛋白替代鱼粉对星斑川鲽幼鱼肌肉水分、蛋白及粗灰分含量未产生显著性影响,肌肉脂肪含量仅S40组显著低于S0和S20组,其他各组间无显著差异;当替代比例小于20%时,肝脏水分呈显著上升趋势(P<0.05),之后趋于平稳(P>0.05)。肝脏粗脂肪变化趋势与此相反。肝脏粗蛋白含量仅表现为S30、S50组显著高于S0组(P<0.05),其他各组无显著差异(P>0.05)。肝脏粗灰分含量不受饲料鱼粉替代比例的影响(P>0.05);全鱼水分呈显著上升趋势(P<0.05),而粗脂肪含量呈显著下降(P<0.05)。S30组全鱼粗蛋白最高,S40组最低(P>0.05),除S30组外,其他各组均与S0组间无显著性差异(P>0.05)。各组间全鱼粗灰分含量变化不显著(P>0.05)。3)当替代比例>20%时,星斑川鲽幼鱼血浆甘油三酯(TG)含量呈显著降低。各组间胆固醇(CHO)含量随紫花苜蓿浓缩叶蛋白的添加呈直线下降趋势(P<0.05)。当替代比例≥20%时,碱性磷酸酶(ALP)活性有下降趋势(P<0.05)。当替代比例分别大于20%和40%时,谷草转氨酶(AST)和谷丙转氨酶(ALT)活性显著升高但各组间差异不显著(P>0.05)。实验鱼血浆溶菌酶(LSZ)活力随鱼粉替代比例的升高呈显著上升(P<0.05)。各实验组间血浆丙二醛(MDA)含量无显著差异,但均低于S0组(P<0.05),超氧化物歧化酶(SOD)活性不受紫花苜蓿浓缩叶蛋白替代鱼粉比例的影响(P>0.05)。结论认为,在本实验条件下,星斑川鲽幼鱼获得最佳生长时紫花苜蓿浓缩叶蛋白替代鱼粉蛋白的适宜比例为19.0%。     

温度、盐度与pH对星斑川鲽呼吸频率的影响

为探讨星斑川鲽的呼吸行为与环境条件的关系,研究了温度、盐度与pH对星斑川鲽呼吸频率的影响。结果显示,在适宜温度范围星斑川鲽呼吸频率随温度的升高而加快,在4～26℃范围内,其呼吸频率Vf与温度T基本呈线性关系Vf=1.8168T+27.23(R=0.9376);在盐度5‰～31‰范围内其呼吸频率变动幅度小,呼吸频率的改变量只有10次/min,为广盐性鱼类;高pH(10.15)和低pH(3.0)情况下,星斑川鲽鱼的鳃盖张合大,鳃盖外缘外翘;30min后表现出不适应,上下窜动,而在pH=3.0中的鱼1h50min全部死亡,可见星斑川鲽耐酸性不如耐碱性。     

环境因子对星斑川鲽呼吸频率的影响

研究了温度、盐度与pH对星斑川鲽呼吸频率的影响。结果显示：在适宜温度范围星斑川鲽呼吸频率随温度的升高而加快,在4—26℃范围内,其呼吸频率V冉温度T基本呈线性关系：Vf=1．8168T＋27．23,R=0．9376;在盐度5—31范围内其呼吸频率变动幅度小,呼吸频率的改变量只有10次,分,为广盐性鱼类;高pH（10．15）和低pH（3．0）情况下,星斑川鲽的鳃盖张合大,鳃盖外缘外翘;30分钟后表现出不适应,上下窜动,而在pH=3．0中的鱼1小时50分钟后全部死亡,可见星斑川鲽耐酸性不如耐碱性。     

星斑川鲽的营养分析与评价

测定了养殖星斑川鲽肌肉中的蛋白质、脂类和灰分等生化组分,并计算了其比能值,分析了肌肉中构成蛋白质的16种常见氨基酸和主要脂肪酸含量。结果表明,星斑川鲽属高蛋白、低脂肪鱼类;肌肉氨基酸含量同其他鲆鲽鱼类相比,属中上等水平;必需氨基酸和呈味氨基酸的含量较高。肌肉蛋白中氨基酸的支／芳值为2．33。各类脂肪酸中,油酸（18：In）含量最高,其次为亚油酸（18：2n6）和棕榈酸（16：0）。星斑川鲽富含EPA和DHA,营养丰富且平衡良好,是值得推荐的优良养殖品种和优质食用鱼类。     

盐度对鲤能量收支的影响

以水蚯蚓为饵料，在温度为２７℃，盐度为淡水、３、５、７、９的条件下对幼鲤（初始体重为２．１９－３．３１ｇ）进行了摄食－生长实验，每一盐度下设４个摄食水平（饥饿－饱足），测定了最大摄食率、吸收率、特定生长率、转化效率和能量收支。结果表明：盐度对最大摄食率、特定生长率（ＳＧＲ）和转化效率有显著影响；盐度对排出废物所占比例（Ｅ／Ｃ）影响不显著，对代谢能所占比例（Ｒ／Ｃ）和生长能所占比例（Ｇ／Ｃ）有显著影     

盐度对饥饿状态下河川沙塘鳢稚鱼生化组成及能量收支的影响

在0.00、1.00、2.00、3.00、4.00、5.00不同盐度条件下,分析和测定了饥饿6 d的河川沙塘鳢稚鱼的生化组成、NH3-N排泄率,并对能量流转进行了研究。试验结果表明,盐度对河川沙塘鳢饥饿稚鱼的生化组成、NH3-N排泄率均有显著影响。盐度2.00,饥饿稚鱼的失重最少,蛋白质、脂肪等能源物质的消耗最少;NH3-N排泄率随着盐度的升高逐渐增高,但在盐度5.00时又稍有下降。盐度对河川沙塘鳢稚鱼在饥饿期间能量收支的各组分有显著影响,其最佳盐度为2.00,在此盐度条件下,河川沙塘鳢饥饿稚鱼损失的生长能最少,为-13.295 J,代谢耗能最低,为11.94 J。     

饲料中添加锌对星斑川鲽幼鱼生长、生理生化指标和机体抗氧化的影响

以酪蛋白和明胶为蛋白源,以七水硫酸锌为锌源,在基础饲料中分别添加锌0、50、100、150、200、400mg/kg,配制成6种等氮等能的精制饲料,饱食投喂初始体重为62.89±0.51g的星斑川鲽幼鱼66d,探讨饲料锌水平对星斑川鲽幼鱼生长、血液生理生化指标和机体抗氧化功能的影响.结果表明,添加150～400mg/kg饲料锌显著提高了星斑川鲽幼鱼的增重率(WGR)(P<0.05),且WGR的最大值及饲料系数(FCR)的最小值均出现在150mg/kg锌饲料组.添加100～200mg/kg饲料锌显著提高了试验鱼血液红细胞数量(P<0.05),0mg/kg锌饲料组的血细胞比容和血红蛋白含量均显著低于其他各组(P<0.05).血清蛋白含量不受饲料锌添加量的影响(P>0.05).血清溶菌酶(LSZ)活力随着锌添加量的增加而显著升高(P<0.05),在锌添加量为150mg/kg时达最高值;当添加量高于150mg/kg时,LSZ活力变化不显著(P>0.05).0与50mg/kg锌饲料组的血清铜锌-超氧化物歧化酶(CuZn-SOD)活力显著低于其他各组(P<0.05).0和50mg/kg锌饲料组肌肉丙二醛(MDA)含量显著高于其他各组(P<0.05),以150mg/kg锌饲料组最低(P<0.05).建议星斑川鲽幼鱼精制饲料中锌的适宜添加量为150mg/kg.     

饲料中DHA/EPA值对星斑川鲽幼鱼生长、体组成及血清生理指标的影响

为研究饲料DHA/EPA值对星斑川鲽幼鱼生长、体组成和血液生理指标的影响,实验配制等氮、等能的5种不同DHA/EPA值(0.64、0.97、1.18、1.59和1.91)的饲料,每个比值设3个重复,饲养周期56 d。结果显示:(1)随着饲料DHA/EPA值的升高,星斑川鲽幼鱼增重率、饲料效率、蛋白质效率均呈先上升后下降的趋势(P0.05)。当饲料DHA/EPA值为0.97~1.59时实验鱼增重最快,饲料效率最高。蛋白质效率则在DHA/EPA值为0.97~1.18时达到最高。蛋白质沉积率(protein retention efficiency,PRE)与饲料DHA/EPA值呈显著二次回归关系(y=-1.589 5x2+2.858 3x+45.184;R2=0.910 8,x=饲料DHA/EPA值,y=PRE),当饲料DHA/EPA值大于0.90时呈下降趋势。肝体比呈先下降后小幅回升的趋势(P0.05),在饲料DHA/EPA值为1.18时达到最低,为2.85%,脾脏指数呈显著上升趋势(P0.05),于饲料DHA/EPA值为1.59组最高(0.12%);(2)肝脏粗脂肪含量随饲料DHA/EPA值的增加呈明显下降趋势(P0.05),且在饲料DHA/EPA值为1.18时降到最低,为8.60%,而后又显著上升,但仍显著低于饲料DHA/EPA值为0.64时的水平(13.44%)。二次回归分析(y=5.199 6x2-15.652x+20.866;R2=0.634 8,x=饲料DHA/EPA值,y=肝脏脂肪含量)显示,当饲料中DHA/EPA值为1.51时肝脏脂肪含量最低。脂肪酸分析结果显示,随着饲料DHA/EPA值的升高,肝脏及肌肉中EPA含量均呈线性下降趋势(P0.05),而DHA含量及DHA/EPA均呈直线上升趋势(P0.05)。肝脏和肌肉组织n-3 HUFA总量不受饲料处理的影响(P0.05);(3)血清总蛋白、球蛋白含量在饲料DHA/EPA值为1.59时显著高于其他各组(P0.05),白蛋白在饲料DHA/EPA值为0.64、0.97和1.59水平最高。溶菌酶(LSZ)活性在饲料DHA/EPA值为1.18时达到峰值(P0.05),为2.76μg/mL。谷丙转氨酶(ALT)活性在饲料DHA/EPA值1.91时无显著变化,而当饲料DHA/EPA1.18时,血清谷草转氨酶(AST)活性提高了65%左右。研究表明,在本实验条件下,以增重率为参考指标,采用二次回归(y=-31.066x2+77.26x+76.541;R2=0.957 4,x=饲料DHA/EPA值,y=增重率)分析可得,当饲料脂肪水平为8.3%,n-3 HUFA含量为0.74%时,星斑川鲽幼鱼[初始体质量(31.70±0.12)g]对DHA/EPA值的最适需要量应为1.24。     

水温和盐度对南美白对虾幼虾能量收支的影响

用室内生态实验法测定了南美白对虾幼虾在不同盐度(5,15或25)和水温(20～23℃,25℃,30℃或33℃)下摄食水丝蚓的能量收支方程。在盐度5～25时,随盐度降低,对虾的能量转换效率升高,同化率降低。在盐度为5时,K1最高(29.02%),同化率最低(77.89%)。对虾具补偿机制来补偿调节渗透压的能耗。盐度对同化率的影响,主要是通过影响呼吸代谢实现的。对虾的特定生长率(y)(%·d-1)随水温(X)(℃)上升而增加,其关系式为:y=0.0168LnX 0.0831(R2=0.9324)。对虾在较高温度下生长快,主要是增加绝对摄食量,提高吸收效率,减少粪便排泄量。在相同温度下,变温时对虾的摄食量高于恒温时。     

饥饿和补偿生长对史氏鲟幼鱼摄食、生长和体成分的影响

报道了饥饿和再投喂对史氏鲟幼鱼摄食、生长以及生化组成的影响.22±2℃条件下,随着饥饿时间延长,幼鱼白肌的RNA/DNA比值不断减小,体重逐渐下降,后者与同期对照组之间存在极显著性差异(P<0.01).饥饿7d,鱼的肝糖原和肌糖原含量显著降低(P<0.05),但随着饥饿时间的延长,肝糖原和肌糖原含量则出现不同程度的回升;脂肪含量和蛋白质含量分别在饥饿14d和21d时下降幅度最大,提示史氏鲟幼鱼动用储存物质的顺序依次是糖原、脂肪和蛋白质.而饥饿过程中鱼体水分和灰分含量则有所上升.恢复投食后,饥饿幼鱼的摄食强度增大,生长加快,其中7d、14d饥饿组幼鱼的RNA/DNA比值达到或接近正常投喂组水平,但21d饥饿组的比值仍明显低于正常投喂组(P<0.05).恢复投食30d后,7d和14d饥饿组幼鱼体重接近对照组(P>0.05),21d饥饿组的终体重未能赶上对照组(P<0.05),这表明史氏鲟幼鱼的补偿生长随饥饿时间不同而异.试验结束时,各处理组鱼体生化组成与正常投喂组没有显著差异(P>0.05).     

Interactive effects of temperature and diet on the growth and biochemical composition of juveniles of the pearl oyster Pinctada mazatlanica

The combined effects of temperature and diet on the growth and biochemical composition of juveniles of the pearl oyster Pinctada mazatlanica at the hatchery were investigated. Specimens were subjected to a combination of four temperatures (20, 23, 26 and 29 °C) and five microalgal diets ( Isochrysis galbana alone, I. galbana + Pavlova salina, I. galbana + Chaetoceros muelleri, C. muelleri + P. salina and I. galbana + P. salina + C. muelleri ). An increase in shell height occurred in a linear pattern, while wet weight gain fitted a power law. Temperature, more than diet, exerted a stronger influence on the growth and condition of the specimens. The combination of 29 °C with P. salina+C. muelleri led to the fastest growth in shell height, while the combination of 29 °C with I. galbana + C. muelleri led to the highest wet and dry weight biomass. In contrast, specimens grew significantly less in shell height and wet weight at 20 °C, regardless of the diet. At all temperatures, the monoalgal diet of I. galbana led to the slowest growth of specimens, but in turn favoured the highest levels of protein, carbohydrate and lipid reserves as a possible strategy to store more energy reserves under stressful events.     

Growth,osmoregulation and energy budget of rainbow and steelhead trout under different salinity acclimation methods and the best transition size of steelhead trout

In order to meet the demand of salmon market, Chinese scientists and entrepreneurs are working on salmon mariculture far offshore in the Yellow Sea, China. Rainbow, steelhead trout and Atlantic salmon were selected as the main culture species. The aims of the present study were as follows: (a) investigate the effect of the salinity acclimation method on the growth, osmoregulation and energy budget in two forms of Oncorhynchus mykiss, rainbow and steelhead trout and (b) explore the optimal size of steelhead trout for the seawater entry. In trial I, rainbow (mean = 99.44 g) and steelhead trout (mean = 99.01 g) were reared for 40 days after undergoing salinity acclimation at three rates: an abrupt increase in salinity from 0 to 30 g/L (T30); an abrupt increase in salinity to 14 g/L, followed by a daily increase of 2 g/L (T2) or 6 g/L (T6) until reaching 30 g/L; and no salinity exposure (control treatment) (T0). In trial II, steelhead trout with body weights of approximately 100 and 400 g were cultured for 60 days with two treatments, T0 and T2, and the specific growth rate (SGR) was calculated every 10 days. In trial I, in both kinds of fish, the optimal growth performance, survival rate, osmoregulation and energy budget were observed in the T0 treatment, followed by the T2 treatment. These results indicate that O. mykiss with a body weight of approximately 100 g can adapt to sea water with a gradual transition (T2), but they are still not suitable for the seawater entry because of low growth. Based on the recorded SGRs in trial II, our formulated regression formula revealed that approximately 200 g is the optimal size of steelhead trout for the transition to sea water.     

Effect of salinity on growth and energy budget of red and green colour variant sea cucumber Apostichopus japonicus (Selenca)

Sea cucumber, Apostichopus japonicus (Selenca), tolerates salinity fluctuations inhabiting intertide zone. This study deals with growth, food intake, food conversion and the bioenergetic responses of the red variant (wet weight of 2.60 ± 0.11g) and green variant (wet weight of 2.56 ± 0.08 g) A. japonicus to different salinities of 22, 26, 30, 34, and 38 psu at 16.5 ± 0.5°C. The results showed that salinity had a significant effect on specific growth rate (SGR) of both green and red variants A. japonicus (P < 0.05). Both colour variants of sea cucumber had highest SGR at 30 psu, and then decreased when salinity below or above this point. Maximum SGR (the green 1.07 ± 0.08% day^?1, the red 1.14 ± 0.09% day^?1 respectively) is related with maximum food intake (FI) and highest food conversion efficiency (FCE) (P < 0.05) occurring at 30 psu. Only under 22 psu, the green variant grew faster than the red variant (P < 0.05), and under other four salinity treatments there was no significant difference between SGR of two colour variant holothruians (P > 0.05). Values of adaptable salinity scope for green and red variants sea cucumber survival are 18.5~39 psu and 20.9~38.6 psu respectively. The average energy budget formula of sea cucumber at 30 psu was: 100C = 6G +42F +3U+49R (C, energy ingested; G, energy for growth; F, energy loss as faeces; U, energy used for ammonia excretion; R, energy loss for respiration). The sea cucumber had maximum energy ingested (C) and highest proportion of energy for growth (G) at 30 psu, and then decreased when salinity is above or below this salinity. Both red and green variants of A. japonicus deposited for growth were very low, and the energy loss in faeces and energy for respiration accounted for the majority of assimilation energy. The result clearly showed that the optimum condition for farming green and red variants A. japonicus, both with respect growth and energy allocation, is the salinity scope of 26 ~ 30 psu.     

Effects of frequency and amplitude of salinity fluctuation on the growth and energy budget of juvenile Litopenaeus vannamei (Boone)

The effects of salinity fluctuation on the growth, intermoult period and energy budget of juvenile Litopenaeus vannamei were investigated. Salinity fluctuation regimes were set in different frequencies of 2, 4 and 8 days and different amplitudes of ±2, ±5 and ±10 g L^?1 from a control salinity of 20 g L^?1. After a 48‐day feeding trial, the intermoult period of shrimp became shorter with increasing amplitude and frequency of salinity fluctuation (P<0.05). Both the frequency and the amplitude of salinity fluctuation had a significant effect on the growth rate of L. vannamei juveniles (P<0.05). At the frequency of 4 days, the highest growth rates occurred at amplitudes of 5–10 g L^?1, whereas the growth rate was the lowest at 10 g L^?1 when the frequency was reduced to 2 days. Feed intake (FI) and assimilation efficiency (AE) of shrimp were also significantly affected by the salinity fluctuation (P<0.05) and matched the growth rate response. The energy expenditures for growth (G), respiration (R), excretion (U) and exuviae (E) to the energy consumed as food (C) were not affected by salinity fluctuation. However, salinity fluctuation significantly affected the percentage of C as faeces (F), with the lowest value occurring at salinity amplitudes of 5–10 g L^?1 and frequencies of 4–8 days. Therefore, salinity fluctuations (every 4 days by ±5–10 g L^?1) result in higher growth rates than constant salinity conditions (20 g L^?1) through greater FI, enhanced feed assimilation and reduced faecal energy loss.     

低盐度对泥东风螺(Babylonia lutosa)稚螺生长与存活的影响

采用盐度渐变和突变2种方法试验低盐度对平均体重3.7 mg、平均壳高(2.24±0.24)mm的泥东风螺稚螺生长与存活的影响。结果表明:盐度28时,稚螺的生长速度最快,盐度降到24时,对稚螺的影响并不明显,摄食基本正常,但活力略有降低;变态7 d的泥东风螺稚螺能够适应高于21的低盐环境,存活基本正常,有比较高的成活率,但对其摄食和生长有一定的影响;当盐度突变至18时,泥东风螺稚螺仍能存活,突变至15时,则不能存活;而盐度渐变至15时,稚螺仍可存活;盐度逐渐降低只可扩大泥东风螺稚螺的存活盐度范围,但其对最适生长盐度范围影响有限。     

不同温度和盐度条件对脆江蓠生长及其生化组分的影响

在不同温度（14℃、17℃、20℃、23℃和26℃）和不同盐度（5、10、15、20、25、30和35）条件下培养脆江蓠（Gracilaria chouae）,观察测定其生长及藻体生化组分的变化。试验结果显示,脆江蓠生长的适宜温度为14～26℃,最适温度为17～20℃,在此温度条件下藻体可以保持最快相对生长速率（relative growth rate,RGR）;温度高于20℃时脆江蓠的生长受到抑制。在生长状态、光合色素和抗氧化等方面,脆江蓠对低温的耐受能力要比高温强。脆江蓠生长的适宜盐度为20～35,最适盐度为30,在此盐度条件下藻体可以保持最快RGR,盐度低于20时脆江蓠的生长受到抑制。高盐度培养条件下脆江蓠在生长状态、光合色素和抗氧化等方面强于低盐度培养条件。