大通县役用马全血铁含量的测定

对大通县35匹役用马全血铁含量进行了测定。结果表明,大通县役用马全血铁含量为29.53mmol/L±4.31mmol/L。公(骟)、母马组之间全血铁含量差异不显著(P>0.05)。     

门源马骨营养状况的检测

对门源县 3 8匹马的骨营养状况指标进行了检测。结果发现 :门源马的血清钙浓度为 2 .68mmol L ,血清无机磷浓度为 1.3 6mmol L ,血清碱性磷酸酶活性为 66.0 4IU L ,血清羟脯氨酸含量为 15 .2 6μmol L ,均处于正常的范围。按性别组间比较 ,公马的血清钙浓度显著高于母马的 (P <0 .0 5 ) ,其他各项指标均无显著性差异 (P >0 .0 5 )。     

大通含野血牦牛10项血清生化指标的测定

对大通种牛场10头含野血牦牛10项生化指标进行了测定.结果表明,血清钠130.20mmol/L±6.88mmol/L;血清钾4.28mmol/L±0.88mmol/L;血清氯124.61 mmol/L±5.54mml/L;血清总钙8.58mnol/L±1.19mmol/L;血清无机磷1.14mmol/L±0.57mmol/L;血清铁33.22μmol/L±32.23μmol/L;血清镁1.63±0.36mmol/L;血清尿素氮12.54±5.75mmol/L;血清肌酐131.72±2.44 nmol/L;血清总蛋白78.63g/L±0.94 g/L.     

西宁市某地绵羊胰脏功能研究初探

为了研究绵羊胰脏功能 ,对西宁市某地 1 0只 1岁～ 2岁绵羊的血液、尿液中的肌酐含量和淀粉酶活性进行了测定 ,结果表明所测绵羊的血清肌酐含量为 57.61 μmol/L±1 7.73 μmol/L ,血清淀粉酶活性为 75.2 8(μmol·s- 1 ) /L± 3 1 .68(μmol·s- 1 ) /L ;尿液肌酐的含量为 2 949.2 1 μmol/L± 42 6.3 4μmol/L ,尿液淀粉酶活性为 61 .92 (μmol·s- 1 ) /L± 5.77(μmol·s- 1 ) /L ;淀粉酶和肌酐清除率 (CAMS/CCR)比值为 1 .60 %± 0 .75%     

大通县役用马血清总蛋白及蛋白组分含量的测定

对大通县青林乡32匹健康役用马的血清总蛋白及蛋白组分含量进行了测定。结果为:血清总蛋白69.30±3.41 g/L,白蛋白34.26±5.73 g/L,球蛋白35.04±4.92 g/L,白/球比值(A/G)0.98±0.47;血清蛋白质组分(%):A 39.85±0.15,a—球蛋白13.44±0.13,β—球蛋白24.63±0.14,γ—球蛋白22.08±0.41。均处于正常值范围内,公(骟)、母马组之间无显著的差异(P0.05)。     

湟源县巴燕乡役用马血清游离羟脯氨酸含量的测定

采用比色法对湟源县巴燕乡35匹（♂14，♀21）役用马的血清游离羟脯氨酸含量进行了测定。结果发现被检马的血清游离羟脯氨酸含量为16.43μmol/L，同时，公（Shan)、母马组之间无显著差异（P＞0．05）。     

大通县环湖型牦牛血清尿素氮含量的测定

采用改进后的二乙酰一肟微量测定法对大通县35头环湖型牦牛血清尿素氮含量进行了测定。结果表明,血清尿素氮含量为11.07 mmol/L±3.51 mmol/L。公、母牦牛组间的血清尿素氮含量无显著性差异(P>0.05)。     

某屠宰场杂交育肥猪胰脏功能研究初探

对某屠宰场150头杂交育肥猪的血液、尿液中的肌酐含量和淀粉酶活性进行了测定。结果表明,所测杂交育肥猪的血清中肌酐含量为110.50μmol/L±20.54μmol/L,淀粉酶活性为258.67 U/L±61.81 U/L;尿液中肌酐的含量为3 094.36μmol/L±397.57μmol/L,淀粉酶活性为196.82 U/L±16.83 U/L;淀粉酶和肌酐比值(CAMS/CCR)为2.72%±1.41%。     

杜洛克杂种仔猪四项血清钙指标的研究

对青海省湟中县多巴镇猪场的20头杜洛克(父本)杂种仔猪的四项血清钙指标进行了测定。结果为:杜洛克二元杂交仔猪,血清总钙,2.40±0.12mmol/L;血清结合钙,1.57±0.20mmol/L;血清游离钙,0.85±0.19 mmol/血清游离钙百分率,35.27±7.64%。杜洛克三元杂交仔猪,血清总钙,2.45 0.24mmol/L;血清结合钙,1.66±0.13mmol/L;血清游离钙,0.80±0.22mmol/L;血清游离钙百分率,32.12±6.64%。且公、母仔猪间四项钙指标差异不显著(P>0.05)。     

青海环湖地区放牧绵羊骨营养状况的研究

为了探讨青海环湖地区放牧绵羊骨营养状况,采用二甲氨基苯甲醛比色法、孔雀绿-钼酸比色法、MTB比色法和磷酸苯二钠法,对青海环湖地区4个县55只2～3岁放牧绵羊血清羟脯氨酸、钙和磷含量以及碱性磷酸酶进行了测定.结果表明,被检放牧绵羊的血清游离羟脯氨酸含量为(15.359±1.655)μmol/L,血清碱性磷酸酶活性为(2.797±0.741)nmol,(L·s),血清钙、磷含量分别为(2.443±0.291)mmoL/L和(1.210±0.390)mmol/L,血清无机磷明显低于正常值(P<0.05).结论说明,被检的55只放牧绵羊处于低磷的营养水平.     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.     

Mechanism of Action of Probiotic Function of Lactobacilli

乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …