共查询到19条相似文献,搜索用时 156 毫秒
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杂交柳优良无性系组培快繁技术研究 总被引:7,自引:0,他引:7
试验以我省从江苏省林科所引种栽培的杂交柳优良无性系903,799,795为试材,对不同外植体材料进行初代培养,培养基筛选。继代增殖与生根培养,以及试管苗移栽试验,经系统观测与分析,其试验结果:(1)以当年春根际萌条为外植体进行初代培养,其污染率最低,有利于快速,高效建立无菌培养系;(2)不同优良无性系对培养基的选择存在较大差异。其中903号无性系能适应较大范围的激素浓度变幅,而799,795号无性系对培养基内激素水平要求较严格。(3)不同优良无性系,不同外植体来源与不同类型外植体材料,其芽的诱导率和增殖倍数有着明显差异性。 相似文献
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尾赤桉无性系DH201组培技术 总被引:1,自引:0,他引:1
通过以Ms改良后对尾赤桉无性系DH201的腋芽诱导、继代增殖、生根培养的试验,确定了尾赤桉无性系DH201组培生产的关键技术:(1)芽诱导:改良MS 6-BA 0.5 mg/L为最有效配方,出芽率高,成本低;(2)继代增殖:改良MS 6-BA 0.5 mg/L KT 0.2 mg/L NAA 0.05 mg/L为最佳配方,增殖倍数高;(3)生根培养:1/2改良MS ABT1 1.0mg/L 蔗糖2%为效果最优配方,生根率高达95%. 相似文献
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对云南省杉木优良无性系组培快繁育苗技术进行了研究,从不同的材料采集和诱导培养、继代增殖培养、壮苗培养、生根培养、炼苗和移栽等方面分析了不同条件下苗木的移栽效果,为杉木无性系组培快繁育苗,提供重要的参考价值。 相似文献
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户太8号葡萄是20世纪90年代陕西户县葡萄研究所选育的优良鲜食葡萄新品种。以户太8号葡萄春季萌发的半木质化嫩枝作为外植体,从试验材料与方法、初代培养、继代培养、炼苗移栽技术等四方面,进行了两年的试验,成功获得了初代培养配方、继代培养配方和炼苗移栽技术,并掌握了适宜的培养环境,初代培养成功率达到36%,继代培养周期25~28 d ,繁殖系数6.2,移栽成活率90%以上。 相似文献
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体细胞离体培养筛选桉树抗青枯病新品种研究 总被引:1,自引:0,他引:1
以尾叶桉胚性愈伤组织为材料,采用病原菌活体接种与添加致病毒素二种方法进行了离体条件下抗青枯病细胞无性系筛选研究。结果表明:采用致病毒素为抗病愈伤无性系选择压力时,所需致病毒素提取液的加入最适浓度为130 m L/L培养基,最适选择时间为培养后15 天;三种活体接种法选择抗病愈伤无性系时,培养基混菌法较为切实可行,加入浓度为50 m L/L菌液培养基为宜,选择时期为培养后10~12 天。愈伤组织的继代年龄对选择剂的加入量与选择效果有较大的影响,以接种后培养3~5 天的材料作为选择对象较好。 相似文献
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Bon MC 《Tree physiology》1988,4(4):381-387
Shoot apex proteins from 11 seedlings, two juvenile clones and three mature grafted clones of Sequoiadendron giganteum (Lindl.) Buchh. were examined throughout the year by SDS-polyacrylamide gel electrophoresis. A membrane-associated protein known as J 16, with an approximate molecular weight of 16 kD, was always detected in juvenile tissue, but was absent in tissue of the mature clones. The J 16 protein was also present in extracts of a mature clone that had been morphologically rejuvenated by micrografting. Protein extracts from the rejuvenated clone, were separated by electrophoresis and assayed for J 16 using an immunoblotting procedure with anti-J 16 serum. This test revealed two bands, J 16 and another protein with a lower molecular weight. The small molecular weight protein was not detected in juvenile clones. 相似文献
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Several techniques have been developed for reinvigorating, rejuvenating or maintaining the juvenility of plants. None of these techniques is as effective as natural rejuvenation whereby the most mature plant parts, the reproductive organs, produce the most juvenile plant parts, the embryos, through gametogenesis and sexual reproduction. The most common criteria for identifying reinvigoration or rejuvenation are based on morphology, morphogenic capacity and the ability to produce cones or flowers. Doubts remain over whether true rejuvenation (reduced ontogenetic age) can be achieved by artificial methods such as serial vegetative propagation, or whether these methods merely provide reinvigoration (reduced physiological age) by temporarily removing environmental and physiological constraints to growth. Rejuvenation and reinvigoration are difficult to distinguish from each other but there is some evidence that artificial methods provide at least partial rejuvenation of plants. This review examines methods for delaying the maturation of juvenile plants and for reducing the ontogenetic or physiological age of more-mature plants. The review discusses theoretical and practical aspects of juvenility, rejuvenation and reinvigoration in clonal forestry, and identifies research areas in maturation that could lead to more-effective methods for establishing high-productivity plantations. 相似文献
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分析了牡丹植株催花后到复壮前练苗的必要性,研究了圃地复壮和盆栽复壮的栽培技术.结果表明:适当修剪枝叶是影响牡丹成活的主导因子之一;精细的肥水管理对催花后牡丹植株快速恢复生机起重要作用;在牡丹的不同栽培品种之间存在着一定的差异.经2年圃地复壮栽培,牡丹能够再度正常开花. 相似文献
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Influencing micropropagation and somatic embryogenesis in mature trees by manipulation of phase change, stress and culture environment 总被引:1,自引:0,他引:1
This review focuses on clonal propagation of mature trees by tissue culture. Most trees have marked phase changes that result in a decline in their potential for somatic embryogenesis or micropropagation. By altering conditions of the source material ex vitro, or by changing in vitro conditions encountered by the explant, rejuvenation and increased propagation can sometimes be accomplished. Various methods of enhancing micropropagation are reviewed, with particular emphasis on manipulations that involve application of osmotic, temperature or hormonal stress. 相似文献
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通过对上海方塔园编号为0126古银杏生长衰萎原因的分析,从改良土壤、修补树洞、支撑加固等方面提出对古银杏复壮措施,并对古树的复壮要点作初步探析。 相似文献
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更新复壮技术对冷箭竹种群密度及地上部分生物量影响的研究 总被引:4,自引:0,他引:4
更新复壮技术对大熊猫主食竹种之一冷箭竹的种群密度和地上部分生物量的影响的研究,结果表明:更新复壮技术主要提高冷箭竹1~3年生的种群密度和地上部分生物量,1~3年生冷箭竹实验区的种群密度为24.4株m~(-2),是对照区的1.88倍;生物量为1064.021kg/hm~2,是对照区的1.49倍。其各器官生物量的排序为竹秆>竹枝>竹叶,并且为大熊猫提供了更多的可利用的食物量,约1007.909kg/hm~2,为对照区的1.40倍。同时,还探讨了不同林分类型和经营措施内冷箭竹的种群密度和生物量,对于林分类型的影响效果为,糙皮桦—冷箭竹林>岷江冷杉—冷箭竹林>红桦—冷箭竹林,对于经营措施来说,则是集约经营最好,中等经营次之,粗放经营较差。总之,更新复壮技术能够延缓冷箭竹的开花枯死,促进新笋萌发,提高竹子生物量,从而缓解大熊猫的食物危机,促进大熊猫的生存繁衍。 相似文献
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本文报导西加云杉优树离体复壮与无性繁殖。低温预处理(2℃,1-30天)促进休眠顶芽破裂和自由生长芽形成。C型外植体为最佳接种类型。连续继代培羊可改进复壮效应,腋芽和不定芽再生率。目前还未找到有效试管内生根法。组培嫩梢扦插生根率可达75%以上。大量部分复壮组培苗已成功移入温室,经园艺生理和外源植物生长调节物质处理,可为生产提供大量扦条,实现快速大量繁殖。 相似文献