Changes in Metallic Cation Accumulation in Tomato (Solanum Lycopersicum L.) Affected by Boron and Potassium

The aim of this study was to determine the combined effects of boron (B) and potassium (K) on metallic cation accumulation, membrane permeability (MP) and growth of tomato (Solanum lycopersicum L.). Four levels of B (0, 5, 10 and 20 mg kg^?1) and two levels of K (0 and 200 mg kg^?1) were applied. B treatments, with or without K, enhanced the MP of leaves and the concentration of metallic cations except for manganese (Mn) and zinc (Zn) concentration as well B concentration. B uptake increased linearly with increasing B treatments. Metallic cation uptakes decreased at the highest B level. Excess B decreased the shoot and root biomass with and without K supply; however, K partially improved the detrimental effect of toxic B on plant growth. It was concluded that supplying K to growth media can be beneficial for alleviating plant growth reduction and imbalances of metallic cations accumulation caused by excess B.     

Diversity of Nordic Landrace Potatoes (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.) Revealed by AFLPs and Morphological Characters

The management of genebank collections of cultivated potato is costly due to the need for in vitro maintenance and virus eradication. Therefore, it is important to set up conservation strategies, which prevent duplicates entering the collections. In this study, 32 Nordic potato landraces were studied for 57 morphological traits and analysed for amplified fragment length polymorphism (AFLP). Most landraces could be distinguished based on the morphological characters, except five accessions. Using five primer combinations to generate 114 reproducible AFLPs, of which 63 (55%) were polymorphic, the five morphologically indistinguishable accessions were placed into two groups with identical AFLP patterns, suggesting that some of the accessions were redundant for long-term preservation. The AFLP data showed that the Nordic collection of potato landraces is composed of genetically different clones, and morphological analysis revealed a wide range of variability. This variability seems to be distributed randomly over the Nordic region since the cluster analysis based on AFLPs and morphological traits revealed no grouping based on the country of origin. Principal component analysis suggests that fewer morphological traits than used in this study will be sufficient to discriminate between different genotypes of cultivated potato (Solanum tuberosum L.). Future possibilities for rationalising potato collections are discussed.     

Impact of liming on utilization of 59Fe‐chelates by lettuce (Lactuca sativa L.)

A pot experiment was conducted to determine the utilization of iron (Fe) by lettuce (Lactuca sativa L. cv. Australian gelber). Iron was applied as ⁵⁹Fe in inorganic and chelated form, particularly biodegradable chelate, ⁵⁹Fe‐EDDS. Two stereoisomeric forms of ethylenediaminedisuccinate: [S,S]‐EDDS and a mixture of EDDS containing 25% [S,S]‐EDDS, 25% [R,R]‐EDDS, and 50% [S/R]/[R/S]‐EDDS, ethylenediaminetetraaceticacid (EDTA) and ethylenediimino bis(2‐hydroxyphenyl)acetic acid (EDDHA) were used as ligands. Lettuce was grown in unlimed and limed quartz sand with nitrate as the sole source of nitrogen. Liming decreased lettuce yields but had no effect on Fe concentrations, indicating that Fe concentrations were a poor indicator of Fe bioavailability within the plant. In unlimed sand, utilization of ⁵⁹Fe from all ⁵⁹Fe‐chelates was on the same level (2.8%–3.6%). In limed sand, only ⁵⁹Fe‐EDDHA maintained the ⁵⁹Fe utilization on a level (3%) comparable to that in unlimed sand. Although the utilization of ⁵⁹Fe from the other chelates decreased to 0.6%–1.1% after liming, Fe concentrations were not affected due to the increased uptake of indigenous Fe. The most biodegradable form of EDDS, namely ⁵⁹Fe‐EDDS(S,S), provided ⁵⁹Fe for lettuce as efficiently as the mixture of ⁵⁹Fe‐EDDS stereoisomers and the ⁵⁹Fe‐EDTA. Utilization of ⁵⁹Fe in inorganic form was 0.5% and 0.03% in unlimed and limed sand, respectively. This study shows that biodegradable ligands are able to serve as chelators to sustain Fe availability in calcareous environments. They may be of use especially in drip irrigation, where ligand accumulations may pose a threat to groundwater quality.     

To be or not to be—the effect of nature conservation management on flowering of Paeonia mascula (L.) Miller in Israel

Mount Meiron Nature Reserve is the largest Mediterranean reserve in Israel. Since its establishment, goat grazing and woodcutting have been prohibited and dense oak maquis has developed by succession. The succession of vegetation poses the risk of losing species. The peony (Paeonia masculla) is a locally endangered species in Israel, growing only in a small population in the Mount Meiron Nature Reserve with only 5% of plants flowering. We sought a management practice that would increase the flowering percentage of the peonies and secure the future of its population. We found that flowering peonies grew mainly in locations with about 47% direct sun radiation, while most plants grew under heavy shade with only 27% direct radiation. Creation of small gaps increased the flowering to 15-20%. To ensure the future of the peonies in the Mount Meiron Nature Reserve, small gaps must be created—even if this conflicts with broader current management policies.     

The Effect of Liming on the Availability of Cadmium in Soils and Its Uptake in Cacao (Theobroma c acao L.) In Trinidad & Tobago

ABSTRACT

Cadmium (Cd) is absorbed and bio-accumulated by cacao (Theobroma cacao L.) trees, resulting in unacceptably elevated levels in cocoa beans, necessitating measures to reduce its uptake from soils. A field experiment, lasting 18 months, was carried out to assess the effectiveness of liming on pH, bioavailability of Cd in soils and its uptake in cacao tissues. The treatments were: (a) control (untreated) and (b) lime-treated trees. Results demonstrated a significant (P < 0.05) increase in the soil pH (lime treated) and a natural fluctuation in pH for the control. For the lime-treated trees, bioavailable Cd levels generally stabilized with no significant change (P > 0.05) compared to the significant (P < 0.05) increase showed by control trees. The Cd levels in the leaves of both treatments decreased, however, the rate of decline in leaf Cd concentrations for lime-treated trees (?0.1378) was 3x faster than control (?0.0497) trees demonstrating the effectiveness of liming.     

Effects of ZnSO4 and Zn‐EDTA applied by broadcasting or by banding on soil Zn fractions and Zn uptake by wheat (Triticum aestivum L.) under greenhouse conditions

Zinc biofortification of staple food crops is essential for alleviating worldwide human malnutrition. Agronomic interventions to promote this should include fertilizer selection and management. A chelated Zn source, Zn‐EDTA, and an inorganic Zn source, ZnSO₄ × 7 H₂O, were applied either by banding or by broadcasting in soil, and Zn fractions in soil and Zn uptake by wheat were determined in a pot experiment. Compared to ZnSO₄ × 7 H₂O, Zn‐EDTA produced higher Zn concentration in grain regardless of application method and even at a lower application rate. Residual Zn fraction was the largest Zn fraction with both ZnSO₄ and Zn‐EDTA amendment. ZnSO₄ banded in soil caused Zn fractions to be restricted to the Zn‐amended soil band and resulted in lower grain Zn concentrations than did broadcast ZnSO₄. Planting wheat slowed Zn fixation by promoting the maintenance of a high concentration of Zn fraction loosely bound to organic matter (LOM‐Zn) in soil. Zn‐EDTA was a better Zn source for Zn biofortification of wheat than was ZnSO₄.     

Effects of N‐fertilization on shoots and roots of rape (Brassica napus L.) and consequences for the soil matric potential

The underlying question of these investigations asked, how and to which extent rape plants react with transpiration and soil water uptake to different degrees of nitrogen fertilization. Therefore repeated campaigns with concurrent measurements of plant surfaces (leaves, stems, pods), diurnal courses of leaf transpiration and root length density of rape plants growing on heavily (240 kg ha^—1), moderately, (120 kg ha^—1), and nil N‐fertilized plots of an experimental field in northern Germany were performed during two growing seasons. Additionally, matric potentials at different soil depths were measured. In the first year (1994) investigations were concentrated primarily on shoot area development and transpiration, whereas in the subsequent year (1995) root measurements were mainly undertaken. Also, the influence of soil management (ploughing, conservation tillage) was taken into consideration. The plots where the shoot measurements were carried out were ploughed in 1994 and rotovated in 1995. Matric potentials were measured in both years in ploughed soil and, for comparison, also in soils with conservation tillage. Shoot area index, as measure of the transpiratory capacity of the canopy, increased on ploughed soil and reached a maximum before flowering. Thereafter it decreased until harvest when the relative amount of green stems and pods was increasing. Then, the measured transpiration rate per pod surface area was equal to, or higher than, the transpiration rate per leaf surface area. Plant surface area was smaller in plots with conservation tillage and decreased generally with decreasing N‐fertilization. Increasing plant surface area was joined by an increasing density of plant canopy. Light interception was thus highest in the plots receiving 240 kg N ha^—1. Although the shading effect may cause a reduction of transpiration per plant, the total plant mass per area generally resulted in a greater water loss from these plots. Roots reached at least 110 cm depth. Root length density was significantly higher in the upper 10—30 cm of soil than at greater depths. Root mass was smaller in soil with conservation tillage than in ploughed soil. Oscillations of soil matric potentials in the diurnal and long‐term periods were highest in the upper 10 cm of soil. Here, they corresponded well with the cumulative diurnal transpiratory water loss. It is concluded that the soil water dynamics depends largely on the distribution of plant roots. As a result, rape plants did not change their specific transpiration capacity as a response to increased nitrogen fertilization. However, the transpiring plant surface and root length density increased the turnover rate of water by a higher plant density per plot. This effect was more pronounced in ploughed than in rotovated plots.