Energy metabolism and protein balance in growing rats fed different levels of dietary fibre and protein.

A study was performed to investigate the effect of different levels of dietary fibre (DF) and dietary protein on visceral organ size, digestibility, nitrogen balance and energy metabolism in rats. Thirty-six male Wistar rats, initial body weight about 76 g were used in a factorial design consisting of three levels of DF (low, 100 g/kg DM: medium, 250 g/kg DM and high, 290 g/kg DM) and two levels of dietary protein (low, 120 g/kg DM and high, 223 g/kg DM). The added fibre source was soybean hulls and Danish fish meal was used as sole source of dietary protein. Measurements of gas-exchange were done on six rats (one group) while urine and faeces were collected individually. The ratio of food/empty body gain increased (P<0.05) with increasing DF and decreasing levels of dietary protein. The weight of the digestive tract was larger (P<0.05) in rats fed the high fibre diet than in those fed the low fibre diet. The digestibility of nutrients and energy decreased linearly with increasing level of soybean fibre (P<0.05). An increased intake of DF was associated with a concomitant loss of protein and energy to faeces. The microbial degradation of NSP and other unabsorbed carbohydrates caused considerably changes in N metabolism of the colon. In rats fed the low protein diets increased levels of DF decreased N excretion in urine and increased N excretion in faeces, while the ratio of retained/digested protein remained constant. When rats were fed the high protein diet protein retention dropped in response to DF both absolute and relative to digested amount, indicating that energy intake could be a limiting factor. Heat production as a percentage of metabolizable energy (HP/ME) was higher (P<0.05) in rats fed the low protein diet than in rats fed the high protein diet, but no significant difference was found among DF levels.     

Effect of dietary medium chain triglyceride on protein and energy utilisation in growing chicks.

1. Protein, fat and energy retentions of chicks fed on diets containing medium chain triglyceride (MCT) and long chain triglyceride (LCT), at 100 and 200 g fat/kg diet, were investigated. Maize oil was used as the LCT source. The MCT used was glyceryl tricaprylate. 2. Body weight gain and food intake were decreased with the diets containing MCT and these effects were greater at the higher fat concentration. Protein retention was also reduced by dietary MCT, although the efficiency of protein utilisation (protein retained/protein intake) was not altered. 3. The values for fat and energy retentions were significantly lower in the chicks fed on the MCT-supplemented diets than in those receiving the LCT-containing diets. Dietary ME values and efficiencies for energy utilisation (energy retained/ME intake) were also reduced by dietary MCT.     

Effect of inclusion of sugar beet pulp,pelleting and season on laying hen performance

1. The effect of three metabolisable energy (ME) concentrations 12.2, 11.3 and 10.5 MJ ME/kg and two forms of diet (mash and pellet) on egg production in summer and winter were studied. The different ME values were obtained by diluting the 12.2 MJ ME/kg diet with sugar beet pulp of 2.88 MJ/kg DM. In a 3rd summer experiment the water intake of hens on these diets was examined. Data were analysed by factorial analysis of variance, and the relationship between performance and dietary energy concentration was examined by regression analysis.

2. The use of low energy diets (10.5 to 11.3 MJ ME/kg) decreased egg production in both seasons, but pelleting diets improved egg production and egg weight in summer. Egg weight was not affected by energy density in winter, but decreased in the summer when energy density increased.

3. In winter, food consumption was not affected by the dietary ME, while in summer food consumption increased when the dietary ME increased. Therefore, energy intake was not the same at all energy densities, but tended to increase as the energy density increased.

4. In summer, pelleting improved food efficiency and egg production and increased water consumption and the ratio of water to food intake. Water intake and water: food ratio decreased with increasing dietary energy concentration.     

Improvement of dietary protein utilisation in chicks by medium chain triglyceride

1. To improve the influence of variation of ME intake, an experiment was carried out using equalised feeding to investigate the comparative effects on protein and energy utilisation in chicks of diets containing medium chain triglyceride (MCT) and long chain triglyceride (LCT). Experimental diets were given at 3 different food intakes, namely, 100, 120 or 147 g/bird/10 d. The diets contained MCT or LCT on an isoenergetic basis. Maize oil and caprylic acid triglyceride respectively, were used as LCT and MCT sources.

2. Body weight gain and food efficiency of chicks significantly increased with the supplement of dietary MCT compared with dietary LCT at all food intakes. Protein retention and the efficiency of protein utilisation (protein retained/protein intake) at all food intakes also significantly increased with dietary MCT, while body fat and fat retention were significantly reduced. Chicks fed the LCT‐supple‐mented diet, on the other hand, had a lower protein retention, but significantly higher fat retention. The value for energy retention and the efficiency of energy utilisation (energy retained/ME intake) were not significantly different between MCT‐ and LCT‐supplemented diets.

3. It was concluded that supplementing MCT to the chick diet would improve body weight gain and protein utilisation while regulating fat deposition compared to the LCT supplemented diet, under equalised feeding conditions.     

Effect of Dietary Fibre on Digestibility and Energy Metabolism in Pregnant Sows

This study was undertaken to provide information on the effect of two levels of dietary fibre (DF) [184 vs. 407 g DF kg^-1 dry matter (DM)] on the digestibility of nutrients and energy metabolism in pregnant sows during different stages of pregnancy. The amount of DF in the high-fibre diet was supplied by dried sugar beet pulp (20%) and oats, wheat bran and grass pellets. The intake of metabolizable energy (ME) was similar for both the low-fibre and the highfibre diet, with an average daily intake of 30.74 MJ. Digestibility of DM, organic matter, protein, fat and energy was significantly decreased by a high DF level, but no effect of the diets was observed on the stage of pregnancy. A high fibre level significantly increased the digestibility of DF, indicating that fibre from sugar beet pulp is more fermentable than fibre from cereals. Protein utilization increased during the pregnancy, but at a very low level, indicating that the protein level in diets for pregnant sows could be lower. Inclusion of extra DF in the diet significantly increased the amount of energy excreted as CH₄. Heat production was affected by diet, although not significantly (28.77 vs. 29.72 MJ day^-1 in the low-fibre and highfibre diets, respectively). The utilization of ME for energy retention was lower for the high-fibre diet as a result of increased hind-gut fermentation. There was similar relation between the net energy of the standard and the high-fibre diet either calculated using the official Danish feed evaluation (0.77) or experimental estimated value (0.81).     

Effect of moderate methionine deficiency on food, protein and energy utilisation in the chick

The effects of feeding a diet marginally deficient in methionine to chicks have been examined. Compared with methionine‐supplemented diets the methionine‐deficient diet caused increased food intake, less efficient food conversion, decreased nitrogen retention and increased uric acid excretion; measurement of plasma uric acid concentration and liver xanthine dehydrogenase activity also indicated a more active state of uric acid synthesis in methionine‐deficient chicks. The increased food intake in response to a moderate deficiency of methionine is commented upon and the implications of the increased synthesis of uric acid in relation to the fate of the increased energy ingested is discussed.     

Dietary fiber for dogs: I. Effects of graded levels of dietary beet pulp on nutrient intake, digestibility, metabolizable energy and digesta mean retention time

The optimal level of beet pulp (BP) inclusion in a meat-based dog diet and the effects of graded levels of dietary BP on fecal excretion responses and mean retention time of marked fiber in the gastrointestinal tract of the dog were evaluated using 30 female English Pointers assigned to isonitrogenous diets containing 0, 2.5, 5.0, 7.5, 10.0 or 12.5% BP (DM basis). Beet pulp replaced portions of dietary cornstarch. Digestibilities of DM and OM decreased by an average of 6% when comparing diets containing BP to the control diet, and quadratic and cubic responses were noted in digestibilities of fiber constituents (lower values at the 7.5 and 10.0% levels, higher values at the 2.5, 5.0 and 12.5% levels). Digestible energy (DE) and ME intakes (kcal/d) were not affected by treatment, but when expressed as a percentage of GE, values decreased (4.8% for DE; 6.2% for ME) linearly with increasing BP levels. Wet weight of feces increased (from 117 to 374 g/d) linearly as percentage of dietary BP increased. Frequency of defecation was higher (P less than .05) for dogs fed the diet containing 12.5% BP than for dogs fed the other diets (5.2 vs mean value of 2.8/24 h). Mean retention time of marked fiber decreased linearly (high value of 23.4 h for the 2.5% BP treatment, low value of 13.0 h for the 10.0% BP treatment) with increased level of BP. Beet pulp levels up to 7.5% of diet DM appear acceptable as a dietary fiber source in a meat-based canine diet.     

Relationship between food consumption and energy and nitrogen utilisation by chicks given varying amounts of standard and leucine-, isoleucine- and valine-deficient diets

The effect of different amounts of food, varying from below to above the rate of voluntary intake, was studied in chicks using a standard diet and diets containing only half the recommendation concentrations of leucine, isoleucine or valine. Irrespective of diet the body weight gain of the chicks increased with increasing food consumption. The body weight gain of chicks given the leucine-deficient diet was similar to that of chicks given the same amount of the standard diet. In contrast, the body weight gains of chicks given either the valine- or isoleucine-deficient diet were significantly less than that of chicks given the same amount of the standard diet. When the food intakes of the birds were similar the differences in body protein and water contents of the birds fed the different diets reflected differences in body weight gains, whereas body fat content was affected in the reverse manner. Retention and retention rate of dietary energy increased as food consumption increased. This was particularly evident in chicks given the isoleucine-deficient diet, followed by those given the valine-, leucine-deficient and standard diets. For all diets, the amounts of nitrogen retained and nitrogen retention rate (N retained/N consumed) increased in line with body weight gain. The metabolisable energy values of the diets were similar, except for the isoleucine-deficient diet which had a significantly higher metabolisable energy value than the standard valine-deficient diets.     

Increasing dietary crude protein does not increase the methionine requirement in kittens

The objective of this study was to determine if the methionine (met) requirement of kittens is correlated with the concentration of dietary crude protein (CP). The study used 48 male kittens in two replications of six 4 x 4 Latin squares, each representing one concentration of met (1.5, 2.5, 3.5, 4.5, 6.0 or 9.0 g/kg diet) with four CP concentrations (150, 200, 300 and 500 g/kg diet) in 2-week periods. Cystine was present in the lowest CP diet at 5.3 g/kg diet and increased as dietary CP increased. Body weight gain, food intake, nitrogen balance and plasma amino acids, glucose, insulin, cortisol, somatomedin C, T(3) and T(4) concentrations on day 12 were measured. From breakpoint analysis of the nitrogen retention curves, the met requirement of kittens was found to be 3.1, 3.8, 3.1 and 2.4 g met/kg for the 150, 200, 300 and 500 g CP/kg diets, respectively. When met was limiting (1.5 or 2.5 g/kg diet), increasing dietary CP did not decrease, but rather increased food intake, body weight gain and nitrogen retention. Plasma met concentrations increased as dietary met increased and at 2.5-3.5 g met/kg diet were not different among kittens fed the various CP diets. Total plasma T(3) and T(4) increased significantly as dietary CP increased in kittens given the 2.5 and 4.5 g met/kg diets. Results indicate that food intake and possibly altered hormonal secretion play a role in this growth response. In conclusion, the met requirement of growing kittens, unlike omnivores and herbivores studied, was not positively correlated with the concentration of dietary CP.     

Growth and haematological response of indigenous Venda chickens aged 8 to 13 weeks to varying dietary lysine to energy ratios

The effect of feeding varying dietary lysine to energy levels on growth and haematological values of indigenous Venda chickens aged 8 – 13 weeks was evaluated. Four hundred and twenty Venda chickens (BW 362 ± 10 g) were allocated to four dietary treatments in a completely randomized design. Each treatment was replicated seven times, and each replicate had fifteen chickens. Four maize–soya beans‐based diets were formulated. Each diet had similar CP (150 g/kg DM) and lysine (8 g lysine/kg DM) but varying energy levels (11, 12, 13 and 14 MJ ME/kg DM). The birds were reared in a deep litter house; feed and water were provided ad libitum. Data on growth and haematological values were collected and analysed using one‐way analysis of variance. Duncan's test for multiple comparisons was used to test the significant difference between treatment means (p < 0.05). A quadratic equation was used to determine dietary lysine to energy ratios for optimum parameters which were significant difference. Results showed that dietary energy level influenced (p < 0.05) feed intake, feed conversion ratio, live weight, haemoglobin and pack cell volume values of chickens. Dry matter digestibility, metabolizable energy and nitrogen retention not influenced by dietary lysine to energy ratio. Also, white blood cell, red blood cell, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration in female Venda chickens aged 91 days were not influenced by dietary lysine to energy ratio. It is concluded that dietary lysine to energy ratios of 0.672, 0.646, 0.639 and 0.649 optimized feed intake, growth rate, FCR and live weight in indigenous female Venda chickens fed diets containing 8 g of lysine/kg DM, 150 g of CP/kg DM and 11 MJ of ME/kg DM. This has implications in diet formulation for indigenous female Venda chickens.     

Low-phytate barley cultivars improve the utilization of phosphorus, calcium, nitrogen, energy, and dry matter in diets fed to young swine

A 28-d experiment was conducted using 45 crossbred barrows with an average initial BW of 9.5 kg and age of 35 d to evaluate low-phytate barley (LPB) mutants (M) M422, M635, and M955, which were hulled, near-isogenic progeny of the normal barley (NB) Harrington and had 47, 66, and 80% less phytic acid, respectively, than NB. A hull-less LPB, M422-H, which was not near-isogenic to the other cultivars, was also evaluated. Apparent nutrient balance, bone measurements, and growth performance were the response criteria evaluated. The barrows were fed the diets to appetite in meal form in individual metabolism crates. Barley and soybean meal were the only sources of phytic acid. Dietary protein supplementation and ME/kg were equalized in all diets. The treatments were diets containing NB, M422, M635, or M422-H without or with added inorganic P (iP), or M955 without added iP. Diets with added iP contained 0.30% available P (aP), the same concentration of aP provided by the diet containing M955 without added iP. There were linear increases (P < or = 0.02) in ADG, G:F, metacarpal and radius bone strength, and fat-free dry weight, and in the absorption and retention (g/d and % of intake) of P and Ca with increasing dietary concentration of aP from the near-isogenic cultivars NB, M422, M635, or M955 without added iP. There were linear decreases in the grams (P < or = 0.02) and percentages (P < 0.001) of P and Ca excreted per day with increasing dietary concentration of aP without added iP. There were no responses for N or energy balance. Growth performance and bone response criteria did not differ for barrows fed the diet containing M955 or the near-isogenic diets containing NB, M422, or M635 with added iP. However, barrows fed the diet containing M955 had greater (P < or = 0.02) percentages of P, N, and energy absorption and retention, Ca absorption, and DM digestibility and had less (P < or = 0.02, g/d and %) excretion of P, N, energy, and Ca (g) per day than barrows fed the diets containing the near-isogenic NB or LPB cultivars with added iP. When dietary aP was equalized with iP, the excretion of P in feces plus urine (g/d) was reduced by 20.2, 27.9, and 44.6%, respectively, in barrows fed the diets containing M422 + iP, M635 + iP, or M955 compared with barrows fed the diet containing NB + iP. Energy utilization did not differ for barrows fed the diets containing hulled or hull-less LPB when ME/kg was equalized with lard. In conclusion, the apparent utilization of P and Ca, the bone strength and fat-free dry weight, and growth performance increased with increasing dietary concentration of aP provided by LPB, in association with linear decreases in P and Ca excretion. Barrows fed the diet containing M955 also had greater utilization and less excretion of P, Ca, N, energy, and DM than barrows fed the diets containing the near-isogenic NB or LPB cultivars with added iP to equalize aP at 0.30%.     

Prediction of nitrogen excretion in feces and urine of beef cattle offered diets containing grass silage

Data from 286 beef cattle, obtained in total diet digestibility assessments, were used to examine effects of dietary and animal factors on N excretion in feces and urine and to develop prediction equations for N excretion in beef cattle. The animals used were mainly from beef breeds, at various ages (from growth to finishing) and live BW (153 to 580 kg), and offered diets containing grass silage at production feeding levels. Dietary forage proportion ranged from 199 to 1,000 g/kg of DM and dietary CP concentration from 108 to 217 g/kg of DM. Linear and multiple regression techniques were used to examine relationships between the efficiency of N utilization and dietary and animal variables with the experimental effects removed. The statistical analysis indicated that N excretion was related positively (P < 0.001) to live BW and intakes of DM, N, and ME, and negatively (P < 0.001) to dietary forage proportion. The prediction equation for N excretion, developed using N intake alone, produced a large r2 (0.898) and a small SE (12.3). Addition of live BW and forage proportion as supporting predictors to this relationship only marginally increased R2 to 0.915 and reduced SE to 11.2. Nitrogen excretion was less well related to live BW (r2 = 0.771, SE = 18.5) than to N intake. Addition of N intake as a proportion of DMI or ME intake to the relationship between live BW and N excretion increased R2 to 0.824 and reduced SE to 16.2. The internal validation of these equations revealed that using N intake as the primary predictor produced a very accurate prediction of N excretion. In situations where data on N intake are not available, prediction equations based on live BW and dietary N concentration together can produce a relatively accurate assessment of N excretion. A number of mitigation strategies to reduce N excretion in feces and urine in beef cattle are discussed, including manipulation of dietary N concentration, diet quality, and level of feeding. The prediction equations and mitigation strategies developed in the current study provide an approach for beef producers to quantify N excretion against production and to develop their own mitigation strategies to reduce N excretion.     

Response of naked neck (Nana) and normal (nana) broiler chickens to dietary energy levels in a subtropical climate

1. The comparative response of naked neck (Nana) broiler chickens and their normally feathered (nana) full sibs to varied dietary energy levels was assessed under natural ambient conditions in a subtropical climate in two separate trials, one in winter (19.0 to 26.0 degrees C) and the other in summer (28.1 to 37.4 degrees C). 2. The chickens were fed on diets with 10.9, 11.5 or 12.1 MJ ME/kg and with a constant protein content of 200 g/kg from 1 to 42 d of age. 3. Both genotypes showed similar body weight, food intake and food conversion efficiency at 42 d of age. Body weight and food conversion efficiency improved on the diet with the highest energy content. 4. Apparent retention (AR) of crude protein (CP) was higher in nana chickens than in their Nana sibs in the winter trial, while no differences were observed for AR of dry matter (DM), crude fibre (CF), ether extract (EE) or calcium. In the summer trial, no difference in nutrient retention was seen between the genotypes. Increasing dietary energy had a positive influence on AR of DM, CP and EE. 5. The relative weights of feathers and skin were lower in Nana chickens than in their normal counterparts. Ready-to-cook yields were greater at the high dietary energy content, while abdominal fat increased with increasing energy content. Nana chickens had more skin protein and less fat in breast muscle and skin than their normal sibs in the summer trial. 6. It was concluded that the Nana broiler chickens showed similar performance and did not differ in dietary energy requirements in comparison to their nana sibs under natural ambient conditions in a subtropical climate.     

Food intake and abdominal adipose tissue in White Leghorn hens fed diets of different protein and energy concentrations

1. Single Comb White Leghorn hens of 2 ages (44 and 80 weeks) were fed diets of different energy (10.88, 12.13 or 13.39 MJ ME and 140 g protein/kg) or protein (120, 140, 160 or 180 g and 12.13 MJ ME/kg) concentration over an 8-week period. 2. Food intake did not change with increasing concentrations of dietary protein. Protein intake was directly correlated with dietary protein concentration. 3. Energy intake increased with dietary energy concentration but, generally, failed to match the increases in dietary energy concentration. Energy, rather than protein, concentration was the major determinant of food intake. 4. Efficiency of energy utilisation decreased and mean adipocyte size increased with higher energy intake. 5. A bimodal adipocyte size distribution, consisting of a primary large size and a secondary small size population, was present in the abdominal fat pad of birds of both ages. There was no significant difference in the numbers of large adipocytes between the hens of the two ages. 6. The greater mean fat pad weight in the older hens was associated with increased mean cell volume in the population of large adipocytes.     

Effect of dietary amino acids and metabolisable energy on the performance of broilers kept at high temperatures

Increasing dietary metabolisable energy (ME) at particular amino acid: ME ratios significantly improved growth and food utilisation of broilers kept at moderate (18 to 26 degrees C) and high (25 to 35 degrees C) ambient temperatures during the finishing period from 22 d of age. The optimum amino acid: ME ratio varied with dietary ME concentration in the hot, but not in the moderate environment. Relatively greater increases in food intake and growth rate occurred in the hot environment when dietary ME was increased and the amino acid: ME ratio was reduced. The minimum rate of food intake did not coincide with the period of maximum temperature. Increasing the dietary protein at particular ME concentrations had little or no effect on the food intake and growth rate of birds kept at high temperatures. Supplementation with dietary fat had no beneficial effect on performance at high temperatures. The rectal temperatures of birds in the hot environment increased with age and, towards the end of the finishing period, when higher energy diets were fed.     

Effect of dietary energy levels on the urinary excretion of purine derivatives in sheep

The effect of dietary energy levels on the urinary excretion of purine derivatives (PD) was investigated in sheep fed hay and concentrate diets. In addition, the possibility of using PD concentrations in spot samples of urine as a useful tool for determining daily PD excretion was also examined. Three female lambs (mean bodyweight (BW) ± standard error, 31.4 ± 1.0 kg) were fed three diets consisting of timothy hay (20 g dry matter/kg BW per day) and rolled barley at three different energy levels (low‐energy (LE), medium‐energy (ME) and high‐energy (HE) at 1.0‐, 1.2‐ and 1.5‐fold greater than the maintenance level of energy (450 KJ/BW^0.75/day), respectively) in a 3 × 3 Latin square design. The dietary protein level was almost constant (380 mg nitrogen/BW^0.75/day as digestible crude protein base) and was adjusted using soybean protein. Digestible organic matter intake (DOMI) increased (P < 0.05) with an increase in the dietary energy level and there was a difference (P < 0.05) in the organic matter digestibility between the LE and HE diets. For urinary PD excretion, the differences between the LE and ME diets and the HE diet were significant (P < 0.05), although there was no clear difference between the LE and ME diets. There was a positive correlation (r = 0.47, P < 0.05) between urinary PD excretion and DOMI. There was also a correlation (r = 0.59, P < 0.05) between the urinary PD level at 7–8 h after the morning feed and the daily total PD excretion, suggesting that the PD level in spot urine samples could be useful for estimating microbial protein synthesized in the rumen and/or digested in the lower gut.     

The influence of recombinant bovine somatotropin on dietary energy level-related growth of Holstein-Friesian bull calves

Our objective for this study was to assess the effect of recombinant bovine somatotropin (rbST) in overcoming the biological effects attributed to live weight increase and age on growth and fat deposition in male cattle. Holstein-Friesian bull calves (n = 56; 182.2 +/- 14.7 d old) were allotted to four subtreatments in a randomized complete block with a factorial arrangement of two levels of rbST: 0 and 500 mg of Posilac, every 2 wk, and two dietary metabolizable energy (ME) concentrations: low metabolizable energy (LME) and high metabolizable energy (HME); 10 vs 11.3 MJ/kg DM, respectively. The effect of rbST treatment on daily gain was expressed mostly on the HME diets. The rbST treatment had no effect on the animals fed the LME diets before the age of 240 d was reached. Dry matter intake and the effect of rbST treatment on DM intake were inversely related to the energy concentration of the diet. The degree of fatness of the animals was significantly reduced by rbST treatment and significantly increased by energy concentration of the diet. The major effect of rbST, under the experimental conditions, in regards to adipose tissue deposition, was on the fat depots and not on the intramuscular fat. The concentration of bST, IGF-I, and insulin in the plasma was increased (P < .001) owing to rbST treatment. Lower metabolizable energy supply led to a higher (P < .001) plasma bST concentration, nonsignificantly lower plasma IGF-I and thyroid hormone concentrations, and lower (P < .001) plasma insulin concentration. A trend (P = .065) toward an increase in PUFA was found in the muscle of the rbST-treated and the HME diet animals. At a young age, when the natural growth potential is high, rbST treatment will be efficient only when a diet allowing a high digestible energy intake is provided.     

Influence of energy intake on growth and utilisation of dietary protein and energy in germ-free and conventional chicks

The effect of metabolisable energy (ME) intake on the growth and utilisation of dietary protein and energy in germ-free (GF) and conventional (CV) chicks was investigated in two experiments. In experiment 1 a high energy diet (HED, 14.8 kJ ME/g) and a marginally-adequate energy diet (AED, 11.7 kJ ME/g) were fed to the GF and CV chicks at 240 g/2 birds/10 d. In experiment 2 a diet with 13.7 kJ ME/g was fed at 118 g (low level, LL) or 128 g (high level, HL)/bird/10 d. Body weight gain, protein retention and protein retention rate were similar in GF and CV chicks on both AED and HED in the first experiment, but in the second were higher in GF than in CV chicks. The increased ME intake of the CV chicks in experiment 2 may be too small to compensate for the increased requirement. ME intake was significantly higher in the CV chicks than in the GF chicks, whereas energy retention was similar in both groups.     

The effect of dietary nutrient concentration and calorie to protein ratio on growth and body composition of male and female turkey poults

Sixteen all‐mash starter diets, with calculated energy levels of 2205, 2646, 3086 and 3527 kcal ME/kg and calorie to protein ratios of about 93, 104, 115 and 126, were fed ad libitum to male and female poults from o to 4 weeks of age. The effect of dietary treatment on growth and body composition was similar for males and females. Growth rate increased by as much as 30 per cent as dietary energy level was increased from 2205 to 3086 kcal ME/kg. This effect was due to changes in nutrient intake associated with almost no change in food intake. A small additional growth response was obtained when energy level was raised from 3086 to 3527 kcal ME/kg but this was mainly associated with an increase in body fat.

Increasing the nutrient concentration and widening the calorie to protein ratio both caused an increase in the percentage of fat and a decrease in the percentage of protein in the body. Nitrogen retention improved as dietary energy level was increased but not beyond 3086 kcal ME/kg.     

饲粮粗蛋白和蛋氨酸水平对育雏期笼养金定鸭性能的影响

采用饲养试验、代谢试验和屠宰试验研究在等能条件下 (ME :1 1 .9MJ kg) ,不同蛋白质水平 (CP :1 7% ,1 9% ,2 1 % )和蛋氨酸水平 (Met CP :0 .0 2 0、0 .0 2 5、0 .0 30 )完全交叉组成的 9种饲粮对笼养金定鸭育雏期 (0～ 4周龄 )生长性能、养分利用、体成分沉积方面的影响。结果表明 :不同蛋白质水平饲粮对试鸭 0～ 4周龄的体增重、采食量、蛋白、能量、钙、磷的利用率以及蛋白、钙、磷沉积量影响显著 (P <0 .0 5或P <0 .0 1 ) ;不同蛋氨酸水平饲粮对试鸭的上述指标 ,除钙利用率、磷沉积量外 ,影响均不显著 (P >0 .0 5)。氮存留率与能量、钙、磷的利用率随饲粮CP(% )的升高而是降低 ,且各水平间差异显著 (P <0 .0 5) ,却随蛋氨酸水平的升高而升高。蛋白水平与蛋氨酸水平的互作结果为 :对氮存留率、能量和磷的利用率影响差异不显著 (P >0 .0 5) ,且以第 3组 (CP =1 7% ,Met CP =0 .0 30 )效果最好 ,而钙的利用率第 3组效果也较好。因此 ,在本试验条件下 ,饲粮适宜粗蛋白和蛋氨酸水平分别为 :CP(% ) =1 7,Met CP =0 .0 30。