孕牛细胞免疫功能的研究

应用酸性α－醋酸萘酯酶染色法、碱性磷酸酶－抗碱性磷酸酶桥联免疫酶染色法（ＡＰＰＡＰ－ＥＬＩＳＡ）和ＭＴＴ比色法分别测量未妊娠、妊娠前期,妊娠中期和妊娠后期。     

乳牛骨软症的调查研究

本文对哈尔滨奶牛场发生的以异嗜、跛行等为特征的乳牛疾病进行了调查研究,分析研究了日粮成分,临床表现,血清钙、无机磷、镁和羟脯氨酸的含量及变化,以及尾椎密度的测定值。结果表明,日粮中钙过多,磷、镁供给不足,钙、磷比例过大是致病的主要原因。并分析研究了乳牛血液生化指标的变化和尾椎骨密度的测定法对诊断和监测骨软症和钙、磷、镁代谢动态的意义。     

CHRONIC COPPER POISONING IN DAIRY COWS

SUMMARY Chronic copper poisoning in a dairy herd fed supplementary copper is recorded. Most cows received 11 g of copper sulphate per day but high milk producers received 22,0 g and within 6 months, 3 of them became ill and died. The signs shown by the 3 cows were anorexia, decreased milk production, frequent recumbency and jaundice. Within 4 days of showing signs, the cows died. The autopsy findings were dehydration, anaemia, jaundice, liver damage and intestinal haemorrhage. Liver copper values varied from 1,250 to 2,410 ppm DM.     

骨钙素在乳牛骨软病研究上的重要性

从北京某农场选取５头骨软病乳牛作为试验组,５头健康乳牛作为对照组,然后进行了血常规,血清学检测。结果表明,试验组乳牛血清钙水平呈下降趋势;血清无机磷水平显著下降。知清ＡＫＰ活性显著升高,其同工酶特征性酶谱中骨带染色谱宽变深;血清维生素Ｄ３活性代谢产物（２５－ＯＨ－Ｄ和１,２５－（ＰＨ）２－Ｄ３）均显著下降;血清骨钙素（ＢＧＰ）水平极显著下降。     

二磷酸组织胺诱发奶牛蹄叶炎病变的病理学研究

本文给临床健康,蹄病成年奶牛８头皮下注射三磷酸组织胺（１５０μｇ／ｋｇ活重）,与对照组５ 期比较。给药前生屠宰前测量两组奶牛蹄前壁长度;屠宰后３３天,对两组占蹄真皮及表皮组织的组织学结构及病理学变化进行了观察,结果显示：实验组奶牛蹄角质的生长速度明显快于对照组,屠宰时测量,两组奶牛蹄前壁的长度差异显著（Ｐ〈０．０５）。组织病理学研究表明：正常蹄表皮组织的角化程序适中,没有完全角化的小叶远端有隔,隔     

乳牛体内维生素D3代谢与骨软病的研究

在北京效区某农场，选择１０头健康乳牛作为对照组，１０头具有骨营养不良的乳牛作为试验组，分别进行了饲料和血清钙，磷含量分析，血清ＡＫＰ活性及其同功酶，血清维生素Ｄ３及活性代谢产物等项目的检测，尾椎Ｘ线检查及维生素Ｄ３治疗试验等。结论如下：①该场乳牛发生的骨营养不良为贩质软化症，维生素Ｄ３其活性代谢产物缺乏是其主要病因；②血清２５－ＯＨ－Ｄ３和１，２５－（ＯＨ）２－Ｄ３是诊断乳牛维生素Ｄ营养状况和乳牛     

AN INVESTIGATION INTO HINDLEG LAMENESS IN DAIRY COWS

A hind leg lameness of dairy cows was investigated in south-east Queensland. Samples of bone, blood, saliva, faeces, urine and milk were collected from lame and normal cows, some of which had received a phosphorus supplement of 30 g per day. Results of these investigations are presented and discussed. Clinical observations and pathological examinations indicated that the disease was an arthropathy affecting either the coxo-femoral or femoro-tibial joints. The disease was present in 30% of herds surveyed. Each owner reported 2 to 3 lame cows. The affected cows could not be differentiated from the normal cows on biochemical grounds. An hypothesis is advanced to explain the reported response of lame animals to large supplements of phosphorus. Information which indicates that previous nutritional status and methods of production cannot be ignored as predisposing factors is presented.     

奶牛发情期的奶孕酮分析

<正> 影响奶牛人工授精成功率的主要原因是对发情期能否正确判断,适时输精。据1981年联邦德国Bavarian动物健康服务机构的报告,输精时间不正确,是影响奶牛繁殖率的重要因素。由于孕酮含量在发情周期中呈规律性变化,在发情期显著下降,因此可     

奶牛头状瘤治疗的免疫研究

采用外科手术部分摘除法、液氮冷冻疗法和瘤体灭活疫苗疗法分别治疗奶牛乳头状瘤各６例，并选用对流免疫电泳和非特异性醋酸萘酯法，结合免疫指标（抗体水平和Ｔ淋巴细胞值）的定期测定，初效，其中以免疫疗法效果最好，保护期最长，是一种实用的治疗方法。奶牛乳头部乳头状瘤自愈，初期是以Ｔ淋巴细胞为主导的细胞免疫为主，后期以抗体介导的体液免疫应答为主导的综合结果