微分光谱遥感及其在水稻农学参数测定上的应用研究

通过不同氮素营养水平的水稻田间试验,解析了水稻冠层微分光谱对消除背景(湿土、水面)信息的影响,证实微分光谱在消除背景信息的影响方面起到了很好的作用。用微分光谱确定出最优的波段宽度应小于10nm;将微分光谱应用于农学参数测定,存在红边位移现象,在孕穗期之前,红边随施氮量增加向长波方向移动“红移”;孕穗期之后“红移”现象基本消失,而发生“蓝移”。红边参数(红边、红边振幅、红边振幅与最小振幅的比值、红边峰值面积)与上层叶片的叶绿素含量、LAI有着密切的关系,而与叶片中的叶绿素b、类胡萝卜素之间相关性不明显。一些红边参数可作为水稻叶绿素含量、LAI测定的简捷方法。从而证实了利用微分光谱测定一些农学参数的可行性     

不同水分处理对烟草叶片高光谱及红边特征的影响

采用ASD Fieldspec HH光谱仪, 测定了不同水分处理下两个烟草品种叶片的光谱特性, 分析其红边特征参数的变化规律及其与烟叶生理指标的相关性。结果表明: 不同水分处理之间的烟草叶片光谱反射率差异明显, 但两个品种的变化规律一致。烟草伸根期和旺长期, 近红外光区的光谱反射率随土壤水分的增加而升高, 成熟期则呈相反趋势, 且光谱反射率在旺长期达到最高值。不同水分处理下烟草叶片一阶微分光谱趋势总体一致, 但红边一阶导数光谱差异显著。红边位置随土壤水分减少向长波方向移动, 发生“红移”现象。红边幅值和红边面积在伸根期和旺长期均随土壤含水量的增加而发生“红移”现象, 成熟期则发生“蓝移”现象。烟草叶片的红边位置同其生理指标的相关性要优于其他参数, 同叶绿素a含量、叶绿素b含量、类胡萝卜素含量、叶绿素总量、叶片鲜重、叶片干重、叶面积和叶片含水率均呈极显著相关关系; 红边幅值和红边面积与叶绿素a含量、叶绿素b含量、类胡萝卜素含量、叶绿素总量和叶片含水率之间均呈极显著性正相关, 红边面积同叶片干重呈极显著性负相关关系。     

基于棉花红边参数的叶绿素密度及叶面积指数的估算

利用野外非成像高光谱仪,测试棉花两个品种4种配置种植方式两年关键生育时期的冠层反射光谱数据,应用光谱微分技术,获取棉花微分光谱680～750 nm波段的红边参数：红边面积（SDr）、红边斜率（Dr）以及红边位置（λr）变量;将棉花红边面积、红边斜率分别与其冠层叶绿素密度（CH.D）、叶面积指数（LAI）进行相关分析,它们的相关性均达到1%极显著水平,其中红边面积与叶绿素密度的相关性最好（RCH.D=0.8787**,n=137）;并且红边面积较红边斜率对叶绿素密度、叶面积指数的预测精度更高。以棉花新陆早13号和19号为建模样本,通过红边面积与叶绿素密度的线性相关模型,分别反演新陆早13号、19号冠层叶片的叶绿素密度,结果表明对这两个棉花品种的叶绿素密度估算精度分别达87.4%和83.3%,说明高光谱红边参数是估算棉花叶绿素密度和叶面积指数的一种简单、快捷、非破坏性的有效方法。     

植被叶片光谱及红边特征与叶片生化组分关系的分析

以LOPEX’93数据集为基础, 利用最新的叶片光学模型——PROSPECT5模型模拟了不同生化组分(叶肉结构参数、等效水厚、叶绿素、类胡萝卜素和干物质)含量叶片的反射率、透射率和吸收率光谱, 并在此基础上提取植被红边特征, 系统分析光谱和叶片生化组分的关系, 建立光谱特征与叶片生化组分的关系模型, 为生化组分反演提供一定的理论基础。结果表明, 在可见光部分, 叶片的光谱主要取决于叶肉结构参数和色素(叶绿素和类胡萝卜素); 在红外波段, 叶片的光谱主要受水分、叶肉结构参数和干物质含量的影响。不同的波段范围内, 光谱对生化组分的敏感度不同。叶片的红边特征不受水分和类胡萝卜素的影响, 但红边位置随叶绿素的增加向长波方向移动(红移)。红边斜率随内部结构参数的增加而增加, 两者之间的关系可用二次方程描述, 这为叶肉结构参数的求解提供了一个方向。     

不同筋力冬小麦冠层反射光谱的差异分析

为了利用冠反射光谱特征监不同筋力小麦品种的生理特征差异,利用不同筋力小麦冠层反射光谱的差异,可对不同小麦品种进行遥感识别与监测。试验以低筋小麦品种扬麦13和高筋小麦品种徐麦31为材料,结合不同生育时期两品种叶面积指数(LAI)、叶绿素含量和叶片氮含量的变化,以及相应的光谱参数,分析不同筋力小麦冠层反射光谱的变化特征。结果表明,在近红外和可见光波段,从拔节期到蜡熟期,扬麦13的冠层光谱反射率均高于徐麦31,在孕穗期两品种的差异最显著;LAI、叶片叶绿素和氮含量均在开花时达最大值,扬麦13的叶绿素含量明显高于徐麦31,而LAI和叶片氮含量则低于徐麦31。比值植被指数(RVI)、归一化植被指数(NDVI)与LAI;红边位置(λr)、红边幅值(Dr)与叶绿素含量,氮素反射指数(NRI)、抗大气植被指数(VARIgreen)与叶片氮含量极显著相关,表明RVI、NDVI可以反演LAI;λr、Dr可以反演叶绿素;NRI、VARIgreen可以反演叶片氮含量的变化。以上光谱参数能反映小麦相关指标的变化情况,不同时期可运用小麦冠层反射光谱进行不同筋力小麦品种识别,孕穗期为最佳识别时期。通过本研究,以期为不同筋力小麦品种的遥感识别提供依据。     

油菜红边特征及其叶面积指数的高光谱估算模型

在2003～2004年油菜生长季,选用6个油菜品种,设置3个氮素水平的小区试验。在不同发育期同步测定油菜冠层的光谱反射率及对应叶片的叶面积指数。利用油菜冠层的光谱反射率数据提取红边参数,分析其变化规律,油菜叶面积指数与红边参数的相关性,估算结果表明：油菜冠层红边一阶导数光谱具有“双峰” 现象,红边位置λ_red位于690～720 nm之间,在油菜生长旺盛期间出现“红边平台”,前期有“红移”,后期有“蓝移”现象;叶面积指数与冠层光谱红边参数λ_red、Dλ_red、S_red之间在开花前存在显著相关,但开花后相关性不显著;利用开花前的红边参数可以估算油菜的叶面积指数,开花后的红边参数不能用于估算油菜的叶面积指数;最后建立了不同时期和开花前油菜叶面积指数的估算模型。     

水稻生物化学参数与高光谱遥感特征参数的相关分析

通过不同氮素营养水平的水稻田间试验，研究稻叶生物化学成份(叶绿素、类胡萝卜素、蛋白质、纤维素、淀粉含量) 与水稻冠层高光谱遥感特征参数之间的关系，结果表明，随着叶位的下移，叶片叶绿素含量与光谱特征变量之间的相关性明显减弱，绿峰反射率、红谷反射率、红边波长、蓝边面积和“三边”面积构成的植被指数等变量与上叶叶绿素含量、类胡萝卜素、纤维素、叶鞘淀粉含量之间有较好的相关性，而蛋白质含量与各种变量的相关系数均非常低。兰边面积、兰边面积和红边面积构成的植被植数与上叶叶绿素含量之间，红边面积 、绿反射峰与红反射峰构成的植被指数与叶鞘淀粉含量之间的相关系数都达到了0.01极显著检验水平，因此，可利用这些变量建立上叶叶绿素a、纤维素和叶鞘淀粉含量的估测模型。     

白术叶片对干旱胁迫的光谱特征响应

白术根茎膨大初期对轻度的土壤水分缺失具有一定的耐受性,但是过度干旱会抑制其根茎膨大及成分积累,因此,无损伤诊断该时期白术是否受到干旱胁迫至关重要。本文以2年生白术为试验材料,在根茎膨大初期控制土壤水分,形成不同程度的干旱胁迫,利用Uni Spec-SC光谱分析仪测定其光谱反射率,并结合光合色素含量,探讨白术叶片光谱特征对干旱胁迫的响应规律,为利用光谱参数监测白术生长状况提供技术依据。结果显示,随着干旱胁迫程度增加,可见光区域(400~750 nm)白术叶片光谱反射率升高,说明其光能吸收利用能力下降;但在750~1 000 nm的近红外波段,光谱反射率则逐渐平稳,在1 000 nm处,干旱胁迫下的全部叶片反射率均低于对照。微分光谱在680~750 nm间差异明显,并与叶绿素含量在700~750 nm间呈显著相关。同时,大多数光谱参数与色素含量呈显著相关(P0.05),尤其类胡萝卜指数(mCRI)、色素归一化指数(PSNDb)、红边位置(λ_(red))、红边幅值(D_(λ_(red)))、红边面积(S_(red))与之呈极显著相关(P0.01)。从上述结果可知,微分光谱680~750 nm可作为检测白术是否受到干旱影响的主要监测波段,红边参数、类胡萝卜指数及色素归一化指数可以作为重要指标,快速、准确、无伤害诊断白术受干旱胁迫程度。本研究结果可为应用反射光谱进行白术干旱胁迫程度诊断提供理论依据和技术支持。     

基于红边参数的水浇地与旱地春小麦各生育期叶绿素最佳估测模型比较研究

通过分析水浇地与不同类型旱地春小麦红边参数变化规律,利用与春小麦叶绿素含量相关性最好的红边位置,分别建立水浇地与旱地春小麦叶绿素含量最佳估测模型。结果表明:春小麦的红边位置表现出水浇地阴坡旱地半阴/阳坡旱地阳坡旱地的特点。起身期到扬花期,各地类春小麦红边位置均偏向长波方向,扬花至乳熟期,又偏向短波。水浇地与阴坡旱地春小麦的红边在起身期和乳熟期存在"单峰"现象,而在其他各期均为"双峰";半阴/阳坡旱地在抽穗和扬花期"双峰"明显,在起身、拔节和乳熟期表现出"单峰"现象;阳坡地在各生育期均为"单峰"现象。整个生育期,水浇地春小麦的红边面积最大,阳坡地的最小。起身期至乳熟期,水浇地与旱地春小麦叶绿素含量线性模型的拟合R2和检验R2均大于0.81,RMSE均小于1.70,说明可以利用红边位置预测各地春小麦叶绿素含量。     

高温胁迫下水稻红边特征及SPAD和LAI的监测

为了促进高光谱遥感技术在水稻长势监测及其灾害评估中的作用,该文以扬稻6号与南粳43为试验对象,通过高温胁迫试验,测定孕穗期高温胁迫后2个品种在不同生育期冠层高光谱反射率、红边参数和叶片绿色度（SPAD）值及叶面积指数（LAI）。结果表明,2个水稻品种剑叶的SPAD值与LAI随生育期的进行呈先上升后下降的趋势,在开花期均达到最大值,在始穗期与蜡熟期时较低。随着高温胁迫的加剧,SPAD值和LAI不断减小。冠层光谱红边位置、红边幅值与红边面积在开花前呈“红移”,开花后呈“蓝移”现象。随着胁迫温度的提高,3个红边参数也呈现“蓝移”现象。不同生育期剑叶SPAD值和LAI与红边特征参数均具有显著或极显著的相关关系。相对于红边幅值,以红边位置为参数所建的模型能更好地预测水稻剑叶的SPAD值和LAI。     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.     

Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater

Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells.     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.     

In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads

We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5-40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg⁻¹ buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg⁻¹ in conditioned and control soil, respectively. The results demonstrate that BioSep^® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation.