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米曲霉中性蛋白酶钝化大豆胰蛋白酶抑制剂 总被引:2,自引:0,他引:2
为了消除大豆饼粕中胰蛋白酶抑制剂的抗营养作用,提高大豆蛋白的利用效率,本文主要从中性蛋白酶加重、酶解温度、Na2SO3加量、不同金属离子和酶解时间等方面考察了米曲霉ZW-06所产中性蛋白酶对豆粕中胰蛋白酶抑制剂的钝化作用.研究结果表明,在豆粕中添加1∶500(质量比)的蛋白酶就能钝化豆粕中40%的胰蛋白酶抑制剂.温度对蛋白酶钝化胰蛋白酶抑制荆的影响很小,金属离子的加入普遍抑制蛋白酶对胰蛋白酶抑制荆的降解,酶解时间在30 min就能达到最大抑制效果. 相似文献
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Mastitis was induced by infusion of 3,000-7,000 colony-forming units of Klebsiella pneumoniae into the left rear quarter of four primiparous Holstein-Freisians. Greater than 100 organisms were recovered from a quarter milk sample within 2 hr and in 87.5% of the hourly cultures taken after 8 hr. Bacteria, California Mastitis Tests, somatic cell counts and N-acetyl-beta-D-glucosaminidase levels differed significantly from that of control quarters in infused cows after hr 6, 8, 9 and 9 respectively. High correlations were obtained between somatic cell count and N-acetyl-beta-D-glucosaminidase (r = .97) and N-acetyl-beta-D-glucosaminidase and natural log bacterial count (r. = .88). N-acetyl-beta-D-glucosaminidase was related to natural log bacterial counts of samples taken 1, 2, 3, and 4 hr prior to the test (rN-acetyl-beta-D-glucosaminidase and natural log bacterial count (r = .91, .92, .93, and .95). 相似文献
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The antigenic attributes of Pasteurella aerogenes sp n were serologically compared with species of the genera Actinobacillus and Pasteurella. Examination included the tube-agglutination and double-immunodiffusion techniques. The results indicated the possibility of serologically different strains of P aerogenes. Antisera prepared from strains of P aerogenes also reacted well with antigens prepared from Yersinia pseudotuberculosis (P pseudotuberculosis) and P pneumotropica. 相似文献
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Three strains of the Pasteurella aerogenes complex were isolated as sole pathogens from aborted fetuses of a sow aborted at the 12th week of gestation on a farm of 600 sows. Gross pathology showed no characteristic lesions. The isolates were biochemically identical and resembled P. pneumotropica on the basis of their strong indole and urease positivity but they produced gas, were ornithine decarboxylase negative and fermented mannitol but not trehalose. Only a few differences were apparent in biochemical characteristics between the isolated strains and P. aerogenes. They differed from the type strain of P. aerogenes in ornithine decarboxylase activity, indole production and lactose and mannitol fermentation; however, such strains do occur within this heterogeneous species. At the time of abortion the antibody titre of the aborted sow was 1 in 16 when examined with live bacterial suspension and 1 in 128 if boiled antigen was used. Similar strains could not be isolated from the vaginas of aborted sows or pregnant and newly farrowed sows in the same group. The bacteriological, serological and histological findings support the opinion of other workers on the occasional pathogenic nature of P. aerogenes. 相似文献
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把采自中华大蟾蜍的蟾酥用蒸馏水稀释成4、6、8、10mg/mL共4种浓度,作为因素A,用LB液体培养基将活化的产气肠杆菌原液按1:10^4和1:10^5稀释,作为因素B,采用二因子(A和B)方差分析观察了蟾酥对产气肠杆菌抑菌圈直径的影响,并分析了蟾酥浓度与抑菌圈直径之间的相关关系。结果显示,蟾酥浓度与抑菌圈直径之间存在显著正相关(r=0.988~0.997);不同浓度的蟾酥溶液对产气肠杆菌均有抑制作用;且随着蟾酥浓度的升高,抑制作用加强。 相似文献
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Boophilus microplus is a rich source of trypsin inhibitors, numerous Kunitz-BPTI (bovine pancreatic trypsin inhibitor) inhibitors have been described from larvae and eggs, named BmTIs. Among them, were characterized inhibitors for trypsin, human neutrophil elastase, human plasma kallikrein and plasmin. BmTIs elicited a protective immunological response against B. microplus infestation in cattle. However, only a small amount of purified natural BmTIs can be obtained from larvae and eggs by chromatographic methods, thus if BmTIs are to be used as vaccine antigens (immunogens) the production of recombinant BmTIs (rBmTIs) is essential. In this work we describe the cloning, expression, purification and characterization of rBmTI-6. rBmTI-6 is a three-headed Kunitz-BPTI inhibitor, expressed in the Pichia pastoris system. Although rBmTI-6 was processed by proteases and glycosylated during the expression process, these post-translational modifications did not alter the ability of rBmTI-6 to inhibit protease activity. Purified rBmTI-6 inhibited trypsin and plasmin. 相似文献
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农业环境可持续性是指减少农业生产活动对环境的影响,从而可以保护我们生活环境的质量,延长农业土地和其它资源的使用"寿命"。酶制剂可以帮助我们实现这一目的。 相似文献
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乐胺磷对5龄期家蚕肠液蛋白酶活性的影响 总被引:2,自引:0,他引:2
乐胺磷是一种兼有触杀作用和胃毒作用的有机磷广谱杀虫剂,在虫害严重的桑田治理中起到较好作用。但由于乐胺磷的作用对象也包括家蚕在内,用药后乐胺磷在桑叶上的残留对家蚕生理生化的影响仍是一个值得探讨的问题。家蚕肠液蛋白酶活性是检测家蚕消化能力的一个标志性酶。本文用紫外吸收法检测了残留量乐胺磷对5龄期家蚕肠液蛋白酶活性的影响。结果显示,在所添食的乐胺磷浓度范围内,乐胺磷添食量与家蚕肠液蛋白酶活性之间呈负相关。在家蚕5龄初期添食乐胺磷引起的肠液蛋白酶活性的下降,至5龄末期已基本消除。 相似文献
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黑曲霉酸性蛋白酶酶学性质的研究 总被引:1,自引:0,他引:1
对黑曲霉菌株ANS1产酸性蛋白酶基本酶学性质的研究表明:酸性蛋白酶适宜反应pH为2.5~4.0,在pH为3.5时,相对酶活力最高;适宜温度为40~50℃,在45℃时,相对酶活力最高;酸性蛋白酶有着较好的热稳定性,曲酶粉在80℃保温5min保留51.4%的相对酶活力,液态酶在80℃恒温水浴中保温60s保留54.6%的相对酶活力,曲酶粉较液态酶有着更好的抗热性;金属离子对酸性蛋白酶活力有一定的影响,在5.0mmol/L的浓度下,Mn2 和Cu2 对酸性蛋白酶有较强的激活作用,Fe3 对该酶表现出明显的抑制作用。 相似文献