猪败血性链球菌与附红细胞体混合感染的诊治

近年来,猪附红细胞体病对养猪生产造成的影响和损失越来越大,该病本身不引起猪只死亡,但因附红细胞体破坏猪血液中的红细胞,使其携氧功能丧失而引起猪抵抗力下降,极易并发感染其它疾病.     

猪败血性链球菌病与附红细胞体病混合感染的诊治

猪链球菌病在临床上较为多见，常表现为败血症、关节炎和局部脓肿。猪附红细胞体病是一种散发性、热性、溶血性疾病，该病常有零星发生或地方流行。这两种病单独发病时治疗时不难，但混合感染后临床症状和单独发病临床症状基本相似，难以诊断和治疗，死亡率高。2004年6月份一猪场发生上述两病混合感染，现将情况介绍如下。     

猪附红细胞体与链球菌混合感染的诊治

从发病情况、临床症状、剖检变化、实验室诊断、防制措施等方面对猪附红细胞体与链球菌混合感染病例进行了介绍,为广大从业者提供参考。     

猪附红细胞体与链球菌混合感染病例

20 0 3年 6月份 ,青岛市某猪场 2 0日龄左右仔猪相继出现抽搐、口吐白沫 ,发烧 ,皮肤苍白症状。用蒽诺沙星等药物治疗无明显效果 ,死亡率达60 % ,后通过综合诊断确认为附红细胞体和链球菌混合感染。通过综合防治很快控制病情。1　临床症状　病猪发病急、蔓延迅速。体温升高至41～ 42℃ ,精神沉郁 ,食欲下降 ,可视黏膜苍白、黄染 ,机体消瘦、脱水。浑身发抖 ,畏寒 ,喜卧、挤堆。部分仔猪呼吸困难 ,有的角弓反张 ,四肢卷缩 ,躺卧于地 ,全身战栗 ,口吐白沫 ,呼吸促迫 ,对外界刺激反应迟钝或无反应。短时间内 ( 2～ 3d)大批死亡。2　病理变化　…     

猪败血性链球菌与附红细胞体混合感染的诊断

猪链球菌病在兽医临床上较为多见,常表现为败血症、关节炎、局部脓肿等.猪附红细胞体是一种散发性、热性、溶血性疾病.     

猪痢疾与附红细胞体混合感染的病例诊治

猪痢疾是由猪密螺旋体引起的一种特有的肠道传染病,临床以消瘦、腹泻或粘液性出血性下痢为特征.该病发病率高、发病快、死亡率高.     

猪链球菌病、猪沙门氏菌病和猪附红细胞体病混合感染的防治

猪链球菌病是猪链球菌感染的一类疾病的总称，猪沙门氏菌病是由沙门氏茵属细菌引起的一种传染病，这两种病是一直困扰我国养猪业发展的主要传染病。猪附红细胞体病是由附红细胞体引起的一种人畜共患传染病。当前我县猪场多处出现混合感染的现象。     

猪附红细胞体病混合猪瘟感染的诊断与防治

2004年5月下旬，我市某个体养猪场在给新购回的一批仔猪做猪瘟三联苗防疫注射后，猪群相继发病，症状以体温升高．精神不振、不食、呼吸困难为主。伴有肢端皮肤发红、发绀及弥漫性出血症状。发病率为44．24％，死亡率为27．43％．经实验室诊断确定为猪附红细胞体病混合猪瘟感染。用血虫净、磺胺等药物注射治疗，同时用猪瘟弱毒冻干苗5倍剂量紧急接种治疗，治疗效果较好。     

猪附红细胞体与链球菌的混合感染病例

猪附红细胞体病是由附红细胞寄生于猪红细胞或血浆中引起的一种血液传染病;猪链球菌病是致病性溶血性链球菌引起猪的一种急性、败血性传染病。这两种疾病已成为断奶猪的多发常见病之一,发病快,死亡率高,并给养殖业带来了巨大的经济损失。2009年7月中旬,某猪场断奶仔猪陆续发病并出现死亡。经综合诊疗为猪附红细胞体与链球菌的混合感染病,现将有关诊断与治疗经过整理如下。     

附红细胞体自然感染猪血液生化指标的检测

附红细胞体 ( Eperythrozoon)是寄生于人畜血液、红细胞及骨髓中的一群微生物。附红细胞体病( Eperythrozoonosis)简称附红体病 ,本病危害的严重性主要表现在感染畜种基本齐全 ,病原分布逐年扩大 ,临床病例逐步增多 ,从 1 92 8年至今的 70多年中 ,在世界范围内已有 30多个国家发生此病。由于该病在多数情况下呈潜伏状态 ,临床发病的不显现性 ,并没有引起人们的足够重视。近年来 ,随着附红细胞体病临床病例的增多 ,家畜附红细胞体感染的暴发流行 ,影响畜牧业发展 ,对人类健康造成威胁 ,才逐渐被国内外学者关注。本试验在前人研究的基础上 ,…     

Indirect hemagglutination for Eperythrozoon suis detection in experimentally and spontaneously infected swine

Ten splenectomized and non-splenectomized pigs were experimentally infected with E. suis bearing red blood cells in order to determine the antibody response. All animals were monitored for antibody titer by indirect hemagglutination over a period of 80-290 days postinfection. Latent E. suis infection only yielded a detectable antibody titer in one pig. Acutely infected pigs had a titer ranging up to 1:640. Maximum antibody response lasted only 2 months and dropped below the level of detection of our assay within 2 to 3 months. At this time, the clinical symptoms could reappear and antibodies were again detectable. However, no booster effect was observed with this second outbreak. We also determined the antibody frequency in 138 pigs from 16 herds in Southern Germany. Pigs from only 4 out of 6 clinically positive herds had antibody titer against E. suis. 20 out of 78 pigs of the clinical positive herds demonstrated a detectable E. suis antibody titer. In 10 herds that were asymptomatic and presumed uninfected all 80 pigs were serologically negative for E. suis.     

猪附红细胞体人工感染小鼠的病理学试验

附红细胞体是寄生于人、猪以及其他动物的红细胞表面、血浆以及骨髓内的一群多形态微生物.猪附红细胞体病缺乏特征性临床症状和病理变化,使发病率和死亡率大大升高,给养殖户造成很大经济损失.本试验旨在研究猪附红细胞体病对小鼠血液生理指标的影响,及对小鼠各脏器的致病程度,为附红细胞体病的诊断及深入研究提供基础试验依据.     

附红细胞体自然感染猪血液流变学

选择临床健康的2月龄附红细胞体自然感染仔猪15头,每周采血1次,连续4次,检测血样的RBC、PCV、ηb10s-1、ηb150 s-1、ηp、ηre10 s、ηre150 s-1、AI、RI、TK.检测结果如下随着猪附红细胞体感染强度的增强,ηb10 s-1、ηre10s-1、ηre150 s-1、AI、RI、TK明显增高,而RBC、PCV、ηb150 s-1、ηp无明显变化,表明猪感染附红细胞体后,血液粘度增高,红细胞聚集性增高,红细胞变形能力降低,进而影响脏器组织的血液灌注和微循环功能.这些血液流变学改变,构成了血栓、炎症、变性、水肿、坏死等的病理学基础.     

附红细胞体自然感染对猪红细胞的影响

选择临床健康的 2月龄附红细胞体自然感染仔猪 1 5头 ,每周采血一次 ,连续 4次 ,观察红细胞的形态 ,并测定RBC、红细胞脆性 (最小脆性和最大脆性 )、红细胞ATPase和SOD活性、红细胞C3b受体 (E_C3bRR)率和红细胞免疫复合物花环 (E_ICR)率。检测结果 :随着猪附红细胞体感染强度的增强 ,所测定指标的变化无明显规律性或无明显差异 (P >0 .0 5 ) ,但红细胞脆性增高、红细胞ATPase和SOD活性及E_C3bRR率和E_ICR率均有降低趋势。表明猪感染附红细胞体后 ,红细胞易破裂而溶血、红细胞内外阳离子平衡失调、红细胞抗氧化能力降低、红细胞免疫功能下降。     

Recombinant DNA probe detecting Eperythrozoon suis in swine blood

A genomic library to Eperythrozoon suis DNA was constructed in lambda gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood DNA, bovine blood DNA infected with Anaplasma marginale, canine blood DNA infected with Ehrlichia canis, feline blood DNA infected with Haemobartonella felis, or uninfected swine blood DNA.     

猪弓形虫病与附红细胞体病混合感染的诊治

本文对垦利县猪混合感染猪弓形虫病与附红细胞体病的流行病学、临床症状、诊断、防治效果等方面作了较全面的介绍.群防群治应用敌菌净(1片/kg体重口服,首次加倍),强力霉素(100mg/kg,拌料喂饲),同时对未驱过肠道寄生虫的猪群,用左旋咪唑(2～3 mg/kg/日,口服),连用3天.很快控制了病情.     

应用间接血凝试验诊断猪附红细胞体病

附红细胞体病 (Eperythrozoonosis)是由附红细胞体 (Eperythrozoon)寄生于红细胞表面、血浆及骨髓内而引起的一种以红细胞压积降低、血红蛋白下降、白细胞增多、贫血、黄疸、发热为主要临床特征的人兽共患病 [1,2 ]。近年来随着人附红细胞体病例的增多 ,家畜附红细胞体病的暴发流行 ,已引起国内外学者的广泛关注 [3 ,4]。本试验探索了将用 IHA于猪附红细胞体病的诊断 ,以便为该病的临床诊断提供一种较为敏感、特异、实用和快速的血清学诊断方法。1　材料与方法1.1　血清样品　猪附红细胞体病阳性血清和阴性血清均由本教研室提供 ,被检血清…     

Epidemiological relationship of human and swine Streptococcus suis isolates

Two cases of meningitis due to Streptococcus suis in humans are reported here. A butcher and an abattoir worker were referred to a health centre in Castellón (Spain) with fever and symptoms of meningitis. After adequate treatment, a slight hipoacusia persisted as sequelae in both cases. Colonies of S. suis group R, serotype 2 and phenotype MRP+EF+ were isolated from cerebroespinal fluid. Epidemiological studies showed that both workers had in common the handling of pork meat of slaughtered healthy pigs from three closed farms. A study of the tonsils from apparently healthy, slaughtered pigs was carried out. A total of 234 tonsillar samples were obtained and 81 strains of S. suis were isolated from them. Serotype 2 appeared to be the most frequent (50.6%), and the analysis for phenotype showed a high percentage of tonsillar strains with the phenotype MRP+EF+ (35.9%). The humans and 28 tonsillar swine strains showed a similar profile (S. suis group R, serotype 2 and phenotype MRP+EF+). A total of 26 of the swine isolates were analysed by ribotyping using EcoRI. The human strains showed the same six-band hybridization pattern that shared five bands with the pattern most frequently shown by most of the tonsillar N. suis group R, serotype 2 and phenotype MRP+EF+ strains, differing only in the lightest, faintest band which was slightly less anodical in human (> or = 1.8 kb) than in swine (approximately 1.8 kb). From these results, both groups of strains, humans and porcine, showed differences; how can these differences in the pattern of ribotyping be explained if they should have the same origin? Is it possible that they have undergone an adaptation to the new host or perhaps the modification is due to other unknown causes? Further studies in this area are required in order to answer these questions.     

猪链球菌病的诊断和防治

1　临床症状及剖检变化某猪场存栏猪 5 0 0 0余头 ,于 1 999年 1 0月发生该病 ,从保育猪舍开始 ,2～ 3天发病蔓延到整个猪场 ,先后发病 82 0头 ,经紧急治疗仍死亡 3 2 5头 ,病死率为 3 9.6 %。表现突然发病不食 ,体温 41～43℃ ,结膜潮红、流泪。有浆液性鼻液 ,呼吸困难 ,腹下、四肢、耳尖等处皮肤呈出血性紫斑 ,少部分育成病猪表现明显的神经症状 ,共济失调 ,倒地四肢做游泳状 ,磨牙或口吐白沫 ,大多于 1～ 3天内死亡 ,症状较轻及治愈者 ,偶留有跛行或生长不良。剖检变化差异较大 ,主要为全身淋巴结充血肿胀 ,脾脏肿大 1～ 3倍 ,边缘发生出…