仔猪副伤寒并发慢性猪瘟混合感染的防控

在长途调运的过程中,副伤寒并发慢性猪瘟是受育肥仔猪的主要死亡原因副伤寒合并慢性猪瘟疾病的发生,多为保护措施不到位和仔猪本身的应激反应造成的。本文将从仔猪副伤寒并发慢性猪瘟混合感染的临床表现、基础治疗及预防入手,提升运输过程中仔猪的存活率并普及本疾病的基本知识点。     

仔猪副伤寒并发慢性猪瘟混合感染的防控

本文主要介绍副伤寒并发慢性猪瘟的发病情况、临床症状、预防、治疗,并且根据实际情况提出了防控措施,以期能为该病的防治提供一定的参考。     

一例仔猪副伤寒并发急性猪瘟混合感染的诊治

介绍某养猪场一例仔猪副伤寒并发急性猪瘟混合感染的发病情况、临床症状、检剖变化、诊断,并提出了防控方法,以为该病的防治提供参考。     

用仔猪副伤寒沙门氏菌自制荧光抗体检测仔猪副伤寒的研究

用兔抗仔猪副伤寒沙门氏菌抗体自制荧光抗体,可在2~3 h内对仔猪副伤寒病作出诊断,比直接镜检更具有特异性和敏感性。     

猪瘟,猪肺疫,仔猪副伤寒混合感染的诊治及发生原因 …

对河北省秦皇岛市某规模化养猪场发生的传染性猪病进行了临床症状及病理变化的调查研究并进行了细菌学检查,动物接种及猪瘟免疫兔体交互免疫试验,提出了防治措施,分析其发病原因主要对仔猪免疫时机及剂量不当,管理系统不完善,环境消毒不严格,没有利用科学的免疫监测方法,细胞弱毒疫苗与组织弱毒疫苗免疫效果有显著差异。     

仔猪副伤寒诊断与防治

仔猪副伤寒是由沙门氏菌属引起的一种严重危害仔猪生产的传染病。该病常发生于6月龄以内的猪,特别是1～4月龄仔猪多发。病程一般在2～23d,有的长达1个月以上。仔猪副伤寒致死率达20%,对养猪业构成严重威胁。现将仔猪副伤寒的诊治与预防总结如下,为仔猪副伤寒的防治提供参考。     

仔猪副伤寒的诊断与防治

仔猪副伤寒是由沙门氏菌属中猪霍乱沙门氏杆菌和猪伤寒沙门氏杆菌引起的以下痢、大肠的坏死性炎症为主要特征的仔猪传染病。主要经消化道传染。     

仔猪副伤寒的诊治

仔猪副伤寒，又名沙门氏杆菌病，是2～4月龄断奶仔猪的常发性疾病。发病的主要原因为在购买仔猪时，往往只给仔猪注射猪瘟和丹毒二联苗，极易携带病菌；再加上猪舍潮湿，饲养条件差等原因，也容易引发仔猪副伤寒。     

仔猪副伤寒的诊断与治疗

仔猪副伤寒为一种仔猪常见多发病,是由沙门氏菌所引起仔猪的一种传染病,故也被称为仔猪沙门氏菌病。该病在我国各地的猪场都有发生,受地域影响明显,本文以福建省永泰县近年来出现的仔猪沙门氏菌病为例,具体的描述以及分析该种病请的发病原因、症状、治疗手段和如何防御的具体方法。     

猪瘟继发细菌性感染的诊断

１９９９年５月，惠水及印江县养猪场爆发一种以急性经过、高热稽留、死亡率高、淋巴结及肾脾出血性变化显著、盲肠末端及回盲瓣附近有钮扣状溃疡等为特征的传染病。为快速、准确地对病猪进行诊断，以提出有效的防治措施，通过流行病学调查、病理剖检、病毒抗原测定及细菌学检验，确诊本次疫病为猪瘟继发细菌感染所致。其中猪瘟病毒荧光抗原的测定结果表明，肠系膜淋巴结、脾和肾的特异性荧光斑点且多亮度最强；肝的特异性荧光较弱。     

仔猪抗E2抗体水平的调查研究

【目的】寻找一种更具针对性、更为可靠的仔猪猪瘟抗体水平检测方法。【方法】分别采用间接ELISA、间接血凝试验（IHA）,对广西某猪场的90份已超免的不同阶段仔猪血清进行抗猪瘟E2抗体和猪瘟全抗体水平监测。【结果】在间接ELISA试验中,阳性样品数为86份,占总样品比例的95．56％;间接血凝试验结果效价在1：16以上（含1：16）的血清样品为88份,占总样品比例的97．78％,两者差异不显著。【结论】仔猪血清中确实存在具有保护性的抗E2抗体,以间接ELISA检测抗E2抗体来监测猪瘟免疫水平是可行的,这为猪瘟免疫水平的监测提供了一种新方法,也为制定免疫程序提供了科学依据。     

重组白细胞介素-2对猪瘟疫苗免疫效果的影响

观察了重组IL-2对经产母猪和育成母猪猪瘟疫苗免疫效果的影响．结果表明，进行猪瘟免疫时应用重组IL-2，可以显著提高经产母猪和育成母猪猪瘟疫苗的抗体滴度水平及整齐度．     

猪瘟抗体ELISA效价与保护力的相关性

对不同猪瘟抗体水平的6个试验组的24头猪及4头对照组猪用已建立的“ELISA间接法检测猪瘟抗体”法进行血清猪瘟抗体ELISA效价的测定,用石门系猪瘟强毒攻击受试猪测定保护力.研究结果表明,血清猪瘟抗体ELISA效价在1:30以上多数能够抵御猪瘟强毒的攻击.该临界线的确定为“ELISA间接法检测猪瘟抗体”技术在猪群免疫监测及防疫注射质量考核中的推广应用提供了依据.     

A multiplex real-time PCR assay for simultaneous detection of classical swine fever virus,African swine fever virus,and atypical porcine pestivirus

With the implementation of the C-strain vaccine, classical swine fever (CSF) has been under control in China, which is currently in a chronic atypical epidemic situation.  African swine fever (ASF) emerged in China in 2018 and spread quickly across the country. It is presently occurring sporadically due to the lack of commercial vaccines and farmers’ increased awareness of biosafety.  Atypical porcine pestivirus (APPV) was first detected in Guangdong Province, China, in 2016, which mainly harms piglets and has a local epidemic situation in southern China.  These three diseases have similar clinical symptoms in pig herds, which cause considerable losses to the pig industry.  They are difficult to be distinguished only by clinical diagnosis.  Therefore, developing an early and accurate simultaneous detection and differential diagnosis of the diseases induced by these viruses is essential.  In this study, three pairs of specific primers and Taq-man probes were designed from highly conserved genomic regions of CSFV (5´ UTR), African swine fever virus (ASFV) (B646L), and APPV (5´ UTR), followed by the optimization of reaction conditions to establish a multiplex real-time PCR detection assay.  The results showed that the method did not cross-react with other swine pathogens (porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease virus (FMDV), pseudorabies virus (PRV), porcine parvovirus (PPV), and bovine viral diarrhea virus BVDV).  The sensitivity results showed that CSFV, ASFV, and APPV could be detected as low as 1 copy mL^–1; the repeatability results showed that the intra-assay and inter-assay coefficient of variation of ASFV, CSFV, and APPV was less than 1%.  Twenty-two virus samples were detected by the multiplex real-time PCR, compared with national standard diagnostic and patented method assay for CSF (GB/T 27540–2011), ASF (GB/T 18648–2020), and APPV (CN108611442A), respectively.  The sensitivity of this triple real-time PCR for CSFV, ASFV, and APPV was almost the same, and the  compliance results were the same (100%).  A total of 451 clinical samples were detected, and the results showed that the positive rates of CSFV, ASFV, and APPV were 0.22% (1/451), 1.3% (6/451), and 0% (0/451), respectively.  This assay provides a valuale tool for rapid detection and accurate diagnosis of CSFV, ASFV, and APPV.     

猪瘟胶体金快速诊断试纸条的研制

采用柠檬酸钠还原法制备胶体颗粒,用猪瘟(CSF)抗体标记纯化,并包被在玻璃纤维膜上,另外将纯化的CSF抗体和羊抗鼠抗体包被在硝酸纤维膜上,组装成CSF快速检测条,对猪瘟病毒进行检测.结果表明:使用所研制的CSF快速检测条检测收集的70份待检样品,共检出猪瘟阳性病例26例,而经病毒分离鉴定阳性病例有28例,阳性符合率达92.8%;将5份阳性猪瘟病毒稀释后用试纸条和ELISA方法进行敏感性实验,结果表明,诊断试纸条的敏感性较高,用试纸条对鸽新城疫病毒、鸡减蛋综合症病毒、猪传染性胃肠炎病毒、猪繁殖与呼吸障碍综合症病毒进行检测,无交叉反应.     

猪瘟病毒荧光定量PCR标准阳性模板的构建

设计扩增猪瘟病毒(swine fever virus,SFV)核酸5’端非翻译区的引物,采用RT-PCR方法克隆目的片段并插入PMD一18 simple T质粒,重组质粒转化大肠杆菌DH5a感受态细胞,提取质粒经PCR和序列测定证实为阳性重组质粒,命名为pSF。将构建的pSF作为标准阳性模板进行荧光定量PCR(FQ-PCR)检测表明,此模板在一20℃保存6个月对结果无显著影响;对于1×10 9,1×10 7,1×10 5拷贝／2μL 的pSF用FQ-PCR方法测定的ct值最大变异系数分别为1．55％;使用该模板建立的定量范围为1×10 2—1×10 11拷贝／2 μL ;构建的阳性重组质粒pSF具有良好的稳定性和特异性。该方法可以用于临床诊断。     

Insights into African swine fever virus immunoevasion strategies

African swine fever(ASF) is an acute and highly contagious disease that causes severe economic losses to the swine industry. ASF is caused by infection of African swine fever virus(ASFV) in domestic pigs, leading to almost 100% mortality. However, no effective vaccines and pharmacologic treatment against ASF are available. ASF poses a severe threat to the swine industry and the economy. Here we summarize potential virus-host cell interaction mechanisms involving the suppression of innate and adaptive immune responses to ASFV entry and infection. These mechanisms include modulation of apoptosis, inhibition of inflammatory responses, reduction of IFN production, inhibition of autophagy, and suppression of MHC-I expression. Insights into immunoevasion strategies by ASFV may shed light on the development of vaccines, as well as preventive and therapeutic drugs.     

猪瘟和猪蓝耳病疫苗临床免疫效果评价

为了切实做好河南省猪瘟和猪繁殖与呼吸综合征疫苗在临床上使用范围、安全性、有效性的调查及免疫效果的评估工作,更好地利用疫苗免疫技术预防猪瘟和猪繁殖与呼吸综合征的发生和流行,在洛阳、新郑等20个市(县、区)、40个猪场进行了猪瘟和猪繁殖与呼吸综合征疫苗的临床应用,并随机抽取了其中338份血清样品,用酶联免疫吸附试验检测其抗体效价。河南省猪瘟免疫抗体合格率平均为73.67%,而猪繁殖与呼吸综合征免疫抗体合格率平均为87.57%。不同厂家疫苗对猪免疫后抗体合格率不同,不同年龄段的猪免疫后抗体合格率不同,不同疫苗种类对猪免疫后抗体合格率不同,对猪免疫次数不同其抗体合格率也不同。以上情况可能与不同厂家疫苗质量不同有关。