Nutritive value of barley/kale bi-crop silage for lactating dairy cows

Six mid‐lactation multiparous Holstein–Friesian dairy cows were used to examine the potential of a fermented whole‐crop barley (Hordeum vulgare)/kale (Brassica oleracea) bi‐crop as a feed compared with a first‐cut perennial ryegrass silage. The barley/kale bi‐crop was grown as a strip intercrop, and was harvested and ensiled as an intimate mixture [0·80 barley and 0·20 kale on a dry‐matter (DM) basis]. Animals were offered ad libitum access to one of three experimental diets in a duplicated Latin Square design experiment: (i) Bi‐crop (the barley/kale bi‐crop); (ii) Grass (the grass silage); and (iii) Mix (a 1:1 fresh mixture of Bi‐crop and Grass). All animals also received a standard dairy concentrate at a rate of 4 kg d^?1 in equal portions at each of two milkings. The Bi‐crop and Grass silages contained 346 and 293 g DM kg^?1, 108 and 168 g crude protein kg^?1 DM, 268 and 36 g starch kg^?1 DM, and had pH values of 3·87 and 3·80 respectively. Animals offered the two bi‐crop silage‐containing diets consumed more forage DM than those offered grass silage (14·6, 14·9 and 12·6 kg DM d^?1 for Bi‐crop, Mix and Grass respectively; s.e.d. 0·45, P < 0·01) and yielded more milk (24·0, 23·9, 22·6 kg d^?1 for Bi‐crop, Mix and Grass respectively; s.e.d. 0·26, P < 0·01). However, differences in the partitioning of dietary nitrogen towards milk protein and away from excretion in urine suggest a more efficient (rumen) utilization of feed protein by animals offered diets containing the bi‐crop silage. It is concluded that, despite having a low crude protein concentration, barley/kale bi‐crop silage offers excellent potential as a feed for lactating dairy cows.     

Effect of three forages and two forage allowances offered to pregnant dry dairy cows in winter on periparturient performance and milk yield in early lactation

Sixty multiparous, Holstein–Friesian pregnant dry dairy cows were allocated to three forage treatments ( n  = 20; fodder beet, kale or grass silage) at two feeding allowances ( n  = 30; high and low) for 70 (s.e. of mean, 16) d before parturition. Cows offered the high feeding allowance were offered 9 kg of dry matter (DM) of kale or fodder beet grazed in situ plus 5 kg DM of baled grass silage daily or clamp grass silage ad libitum offered indoors. Cows offered the low feeding allowance were offered 6 kg DM of kale or fodder beet grazed in situ plus 3·5 kg DM baled grass silage daily, or 9·5 kg DM of clamp grass silage daily offered indoors. After calving, all cows received a daily allowance of 14 kg DM perennial ryegrass herbage at pasture plus 4 kg concentrate cow⁻¹ for the first 35 d of lactation. Cows offered grass silage had a greater increase in body condition score pre-partum compared to those offered kale or fodder beet. Cows offered fodder beet pre-partum had a greater milk solid and solids-corrected milk yield in the first 35 d of lactation than those offered kale and grass silage pre-partum. Offering fodder beet and kale pre-partum increased plasma non-esterified fatty acid concentrations pre-partum relative to offering grass silage. Offering kale pre-partum resulted in higher insulin-like growth factor-1 concentration post-partum but lower plasma copper concentration pre-partum and at calving than kale or grass silage. Offering the higher forage allowance pre-partum resulted in a higher plasma calcium concentration at calving and higher plasma non-esterified fatty acid concentration post-partum.     

Increased concentration of water-soluble carbohydrate in perennial ryegrass (Lolium perenne L.). Evaluation in dairy cows in early lactation

Twelve multiparous Holstein–Friesian dairy cows in early lactation were used to investigate the potential of using perennial ryegrass (Lolium perenne) with a high concentration of water‐soluble carbohydrates (WSC) to increase the efficiency of milk production. Ad libitum access to one of two varieties of zero‐grazed herbage was given continuously for 3 weeks: treatment High Sugar (HS), an experimental perennial ryegrass variety (Ba11353) bred to contain a high concentration of WSC, harvested in the afternoon; or Control, a standard variety of perennial ryegrass (cv. AberElan), harvested in the morning. All dairy cows also received 4 kg d^?1 of a standard dairy concentrate. Dairy cows given the HS diet treatment consumed 2·8 kg dry matter (DM) d^?1 more than Control dairy cows (P < 0·01), and the DM digestibility of the diet on the HS treatment was significantly greater than that of the diet on the Control treatment (0·75 vs. 0·72; s.e.d. 0·010; P < 0·05). Excretion of urinary purine derivatives (PD) tended (P < 0·1) to be higher from dairy cows on the HS treatment, implying increased microbial protein flow to the duodenum, although there was no significant difference in the apparent efficiency of rumen fermentation of either dietary nitrogen (N) or DM expressed as a ratio to urinary PD. Milk yields and milk composition were not significantly affected by dietary treatment, although true protein yields of milk were higher (P < 0·05) from dairy cows given the HS treatment. The proportion of dietary N excreted in urine was significantly lower from HS cows, although the values were low for both treatments (0·20 g g^?1 vs. 0·27 g g^?1; s.e.d. 0·020; P < 0·05). It is concluded that increased DM intakes by dairy cows given the HS treatment led to increased milk protein outputs. With a proportional decrease in urinary N excretion, the use of perennial ryegrass with a high WSC concentration, in the context of the harvesting regime used in this study, may help to reduce N pollution from dairy systems into which it is incorporated.     

Effects of feeding clover silages on feed intake, milk production and digestion in dairy cows

Silages were made from pure crops of perennial ryegrass, red clover and white clover over 2 years. In all but one case the silage was stored as bales. A silage additive specially adapted for bales (Kofasil Ultra^TM) was used for all silages and they were all of good hygienic quality. The additive contained sodium nitrite, hexamethylene, tetraamine sodium bensoate and sodium propionate. The silages were offered ad libitum, either pure or mixed [grass/clover 0·50/0·50 on a dry‐matter (DM) basis] with a fixed amount (8 kg) of concentrate. Two experiments, one in each year, were performed with high‐yielding multiparous dairy cows in mid‐lactation, and both rumen‐cannulated and intact cows were used. The experiments were carried out using an incomplete changeover design with fifteen cows and five treatments each year. The cows consumed large quantities of these silages (12·7–16·3 kg DM per cow per day). The highest intakes were obtained when the red clover and the 0·50 red clover:0·50 perennial ryegrass silage diets were offered. However, there was a difference between years. In year 1, 0·50 red clover:0·50 perennial ryegrass and 0·50 white clover:0·50 perennial ryegrass silage diets showed the highest intakes while pure perennial ryegrass and white clover silage diets gave lower intakes. In year 2 the highest intake of silage was obtained when the diet containing silage from red clover from a second cut was offered, while the silage from red clover from a first cut gave the lowest intake. The voluntary intakes of silages from white clover and perennial ryegrass were intermediate. No cases of bloat or other digestive disturbances were observed. Milk yield was significantly lower for the perennial ryegrass silage diet compared with all other diets in year 1. In year 2 milk yield was highest for the white clover silage diets and lowest for the red clover silage diets from both cuts. In year 1 there were relatively small differences in milk composition while in year 2 milk fat content was significantly lower with white clover silage diet and milk protein content was significantly higher with the perennial ryegrass diet. The overall conclusion from these experiments was that cows were able to consume large quantities of pure legume silage without serious disturbance to their metabolism. Differences in measurements of rumen metabolism were found between diets and especially between years. Milk production differences appears to be coupled to both differences in rumen physical characteristics, such as passage rate and particle size as well as differences in volatile fatty acid production in the rumen.     

Increased concentration of water-soluble carbohydrate in perennial ryegrass (Lolium perenne L.): milk production from late-lactation dairy cows

Eight multiparous Holstein–Friesian dairy cows in late lactation were used to investigate the potential of using perennial ryegrass with a high concentration of water‐soluble carbohydrate (WSC) to increase the efficiency of milk production. After a pretreatment period on a common pasture, the cows were each given ad libitum access to one of two varieties of zero‐grazed grass continuously for 3 weeks. Treatments were: high sugar (HS), an experimental perennial ryegrass variety bred to contain high concentrations of WSC; or control, a standard variety of perennial ryegrass (cv. AberElan) containing typical concentrations of WSC. The two grass varieties were matched in terms of heading date. All animals also received 4 kg day^–1 standard dairy concentrate. Grass dry matter (DM) intake was not significantly different between treatments (11·6 vs. 10·7 kg DM day^–1; s.e.d. 0·95 for HS and control diets respectively), although DM digestibility was higher on the HS diet (0·71 vs. 0·64 g g^–1 DM; s.e.d. 0·23; P < 0·01) leading to higher digestible DM intakes for that diet. Milk yield from animals offered the HS diet was higher (15·3 vs. 12·6 kg day^–1; s.e.d. 0·87; P < 0·05) and, although milk constituent concentrations were unaffected by treatment, milk protein yields were significantly increased on the HS diet. The partitioning of feed N was significantly affected by diet, with more N from the HS diet being used for milk production (0·30 vs. 0·23 g milk N g^–1 feed N; s.e.d. 0·012; P < 0·01) and less being excreted in urine (0·25 vs. 0·35; s.e.d. 0·020; P < 0·01). In a separate experiment, using the same grasses harvested earlier in the season, the fractional rate of DM degradation, measured by in situ and gas production techniques, was higher for the HS grass than for the control. It is concluded that increased digestible DM intakes of the HS grass led to increased milk yields, whereas increased efficiency of utilization of the HS grass in the rumen resulted in the more efficient use of feed N for milk production and reduced N excretion.     

Effect of swath treatment on water loss during field-wilting and on feeding value of perennial ryegrass silage

The effect of spreading mown perennial ryegrass (Lolium perenne) herbage over the total ground area on water loss during field-wilting was compared with leaving herbage in swaths (three swaths put together into one, occupying 0·18 of ground area) in three experiments. Spread crops were not tedded during wilting but were rowed up immediately before harvest. In all experiments, conventional silage-making equipment was used on a field scale. Feeding value was assessed with lactating dairy cows and growing heifers (Experiment 1) and sheep (Experiment 3). The periods of field wilting were 48 h (Experiment 1), 24 h (Experiment 2) and both 24 h and 48 h (Experiment 3). Spreading the crop was associated with larger increases in loss of water in all three experiments compared with leaving grass in swaths. Losses of dry matter (DM) during wilting were similar in Experiment 2 but were higher for the swathed crop wilted for 48 h than for 24 h in Experiment 3. Spreading resulted in restricted fermentations associated with higher crop DM contents at ensiling. In Experiment 1 the concentrations of DM, ash and water-soluble carbohydrate in silage were higher (P < 0·001) for spreading the crop and the concentrations of crude protein and neutral-detergent fibre were lower (P < 0·05) than for swathed material. In Experiment 3, spreading was associated with higher concentrations of water-soluble carbohydrates and ethanol and lower concentrations of fermentation acids, ammonia-N and neutralizing value in silage. Voluntary DM intake of silage by dairy cows and heifers was higher for spread than for swathed material (P < 0·05), but in Experiment 3 (sheep) there were no significant differences between treatments in voluntary intake of DM. The increased intake by dairy cows of silage from spread herbage was reflected in increased concentrations of milk fat (P < 0·01) and protein (P < 0·05) but not in milk yield (P > 0·05). It is concluded that spreading herbage during field wilting prior to ensiling accelerates water loss and has the potential to improve the feeding value of the ensiled product.     

Release of cell contents and comminution of particles of perennial ryegrass herbage during ingestion by dairy cows fed indoors or grazing

The effect of feeding indoors fresh perennial ryegrass vs. grazing on ingestive behaviour, release of cell contents and comminution of particles during ingestion, as well as on gas production of ingested boli fermented in vitro, was studied. Indoor feeding and grazing were compared using four dairy cows according to a triple reversal design with six periods. Chemical and morphological composition of the ingested herbage was similar for both indoor feeding and grazing treatments. The intake rate was markedly higher indoors compared with grazing [52·1 vs. 22·9 g dry matter (DM) min^?1] with heavier boli and less saliva added per gram of DM intake. The proportions of intracellular nitrogen and chlorophyll released during mastication after ingestion of herbage fed indoors were lower, and the median size of the particles in the boli was larger (5·97 vs. 4·44 mm) compared with grazing. As a result, the rate of gas production in vitro was also lower for herbage fed indoors compared with grazing (0·423 vs. 0·469 mL min^?1 g^?1 incubated DM). Indoor feeding or grazing may have limited consequences in vivo on the kinetics of availability of nutrients for micro‐organisms in the rumen, because the consequences of the more extensive physical damage suffered by herbage ingested at grazing could be compensated by a lower intake rate.     

Characteristics of baled silage made from first and second harvests of wilted and severely wilted forages

First and second harvests of lucerne (Medicago sativa L.), perennial ryegrass (Lolium perenne L.) and a lucerne–perennial ryegrass mixture [80 or 144 g kg^?1 dry matter (DM) of ryegrass] at the first and second harvests were cut and conditioned, wilted to 500 or 700 g DM kg^?1 then baled and stretch‐wrapped for silage on the same dates. Lucerne bales were denser (411 kg m^?3) than bales of perennial ryegrass (331 kg m^?3) (P < 0·05). After an 8‐month storage period, silage made from high DM‐content forage had a higher concentration of neutral‐detergent fibre (NDF) and was less digestible than that made from low DM‐content forage. Daily DM intakes by beef steers, when the silages of the second harvest were fed ad libitum, were 31·2, 31·2 and 22·3 g kg^?1 live weight for lucerne, lucerne–perennial ryegrass mixture and perennial ryegrass silages, respectively (P < 0·01), when the herbage had been wilted to 500 g kg^?1. In vivo digestibility of NDF in the lucerne–perennial ryegrass mixture silage (0·587) was significantly lower than that of perennial ryegrass silage (0·763) but higher than lucerne silage (0·518). Higher intakes of baled lucerne silage tended to offset its lower digestibility values. Lucerne–perennial ryegrass mixture silage had a higher DM and NDF digestibility than lucerne silage, indicating perhaps the presence of associative effects.     

Effect of early turnout to grass in spring on dairy cow performance

This experiment examined the effects of grazing severity and degree of silage restriction during early turnout of dairy cows to pasture in spring on animal performance. Forty late‐winter‐calving Holstein Friesian dairy cows were allocated to one of five treatments between 7 March and 17 April 1997. The treatments involved early turnout of cows to grass for 2 h per day at two residual sward heights and two silage allowances, plus a control treatment, in a randomized block design. Dairy cows on the control treatment remained indoors throughout the experiment and were offered grass silage ad libitum. Dairy cows on all treatments were also offered 6 kg d^–1 of a concentrate on a flat‐rate basis, split equally between the morning and afternoon milkings. Offering cows access to pasture in early spring for 2 h per day resulted in increases in both milk (P < 0·001) and protein yield (P < 0·01). On average, over all grazing treatments, cows produced an additional 2·6 kg milk per day compared with the control treatment (28·5 vs. 25·9 kg d^–1, s.e.m. 0·43). Furthermore, these increases in milk yield were obtained even when silage was restricted indoors (28·4 vs. 25·9 kg d^–1) and cows grazed down to a residual sward height of 40 mm (28·1 vs. 25·9 kg d^–1). Protein yield was higher (P < 0·01) with dairy cows grazing pasture compared with cows indoors (848 vs. 707 g d^–1, s.e.m. 28·9). Silage intake was significantly (P < 0·001) reduced when cows were turned out to pasture. In conclusion, early turnout of dairy cows to pasture in spring for 2 h per day reduced silage intake and increased milk yield and protein yield relative to those fully housed and offered grass silage with a low level of concentrates.     

Selection by sheep and goats for perennial ryegrass and white clover offered over a range of sward height contrasts

The selection by sheep (six Coopworth ewe hoggets, 44·3 ± 4·6 kg live weight) and goats (six Saanen/Anglo‐Nubian yearling males, 38·1 ± 3·8 kg live weight) for perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) and for sward height was measured in two experiments involving paired turves. Pairs of turves with herbage of differing height and of either the same or different plant species were offered. One sward (fixed height species, FHS) was always offered at 130 mm and the other (variable height species, VHS) at 130, 90 or 50 mm. Turves (450 mm × 220 mm) were cut to a soil depth of 100–150 mm from areas of perennial ryegrass and white clover regrown to the desired height after previously being cut to 30 mm. Each turf in a pair was weighed (±1 g) before and after grazing by penned animals maintained on a barley‐based pelleted diet. The number of prehending bites taken from each turf was recorded over a grazing period (128 ± 12 s). Bite mass, bite rate and intake rate were calculated. As the sward height of the VHS turf declined, an increasing proportion of the diet was selected from the 130 mm turf. When averaged over all height contrasts, both animal species selected a higher proportion (0·776 ± 0·026) of their diet from 130‐mm white clover than from 130‐mm perennial ryegrass (0·591 ± 0·018) turves. On average, goats selected a higher proportion (0·721 ± 0·022) of their dry‐matter (DM) intake from the 130‐mm turf than sheep (0·646 ± 0·019), but the effect was not consistent. In contrasts with perennial ryegrass as the VHS (and both perennial ryegrass and white clover as FHS), the proportion of the diet selected from the 130‐mm turf was very similar for both animal species. However, with white clover as the VHS (and both perennial ryegrass and white clover as FHS), goats selected a higher proportion of their intake from the 130‐mm turf to the extent that in the 130‐mm perennial ryegrass/50‐mm white clover contrast sheep showed as strong selection for 50‐mm white clover as goats did for 130‐mm perennial ryegrass. This lesser selection of goats for white clover as its height in a sward declines is likely to contribute to the higher white clover content observed in swards grazed by goats. Bite mass was greater on white clover (246 ± 5 mg DM bite^–1) than on perennial ryegrass (173 ± 5 mg DM bite^–1) and was greater for goats (255 ± 6 mg DM bite^–1) than for sheep (195 ± 5 mg DM bite^–1). Bite rate was greater on perennial ryegrass (45·9 ± 1·0 bites min^–1) than on white clover (39·9 ± 1·0 bites min^–1) and was greater for sheep (45·5 ± 1·1 bites min^–1) than for goats (42·5 ± 1·1 bites min^–1). Apparent intake rate by both sheep and goats was lower (mean, 5·0 ± 0·29 g DM min^–1) on 130 mm perennial ryegrass/white clover than on 130 mm perennial ryegrass/perennial ryegrass (7·0 ± 0·27 g DM min^–1), but was higher (9·62 ± 0·29 g DM min^–1) on 130‐mm white clover/perennial ryegrass than on 130‐mm white clover/white clover (8·2 ± 0·29 g DM min^–1) combinations.     

Effects of a stay-green trait on the concentrations and stability of fatty acids in perennial ryegrass

Abstract Three experiments were conducted to evaluate the effect of a stay‐green trait in perennial ryegrass (Lolium perenne) on concentrations of fatty acids as well as their susceptibility to peroxidation during wilting and to biohydrogenation by rumen bacteria. Fatty acid concentrations were recorded in stay‐green and corresponding normal perennial ryegrass selection lines over eight cuts during 1998. There was a progressive increase in total fatty acid concentrations [from 20·8 to 34·6 g kg^?1 dry matter (DM)] and the proportion of fatty acids as α‐linolenic acid (from 0·62 to 0·70 g g^?1) from early to late season. A second study compared fatty acid concentrations in stay‐green and normal herbage that was wilted for up to 48 h. There was a loss of 0·2–0·3 g g^?1 fatty acids during 48 h of wilting and a small reduction in the rate of loss of α‐linolenic acid in stay‐green perennial ryegrass compared with normal herbage (0·223 vs. 0·290 g g^?1 lost after 48 h). Stay‐green and normal perennial ryegrasses were offered to grazing lambs in a third study. Higher concentrations of trans‐vaccenic acid and conjugated linoleic acid in plasma from lambs offered less mature grass in the pre‐experimental period than during the experiment are considered to reflect a greater supply of precursor (linoleic acid). There were higher concentrations of conjugated linoleic (0·0070 vs. 0·0039 g l^?1) and linoleic (0·092 vs. 0·070 g l^?1) acids, without an increase in trans‐vaccenic acid, in plasma from lambs grazing stay‐green perennial ryegrass than normal perennial ryegrass. This suggests that the stay‐green trait affected the rate of degradation of fatty acids in the rumen. These results demonstrate the potential for obtaining proportionately large differences in fatty acid profiles of ruminant products by altering grassland management.     

The performance of summer-calving cows grazing perennial ryegrass swards

The objectives of this experiment were to study the effects of different grazing managements in spring on herbage intake and performance of summer-calving dairy cows and to examine the effects of regrowth in early June on herbage intake and cow performance. Four spring-grazing treatments were applied to predominantly perennial ryegrass swards: Control (C), sward grazed by cows to 6–8 cm sward surface height (SSH); CG16, sward grazed by cows to 3–4 cm SSH in May and allowed to regrow to a target SSH of 16cm in early June; CG8, sward grazed by cows to 3–4 cm SSH in May and allowed to regrow to 8cm in early June; and SG8, sward grazed by sheep to 2–3 cm SSH in May and allowed to regrow to 8 cm in early June, All swards were continuously stocked by summer-calving (May and July) primiparous and multiparous cows from 16 June to 7 September, to a target SSH of 8–10cm. Spring treatments bad marked effects on herbage intakes and milk production. Estimated in July by n alkane analysis, the mean herbage intake ± s.e.d. of cows on each treatment were 1·8, 1·4, 1·4 and 3·0 ± 0·31 kg dry matter (DM) 100 kg live weight (LW)^?1 d^?1 (P < 0·01) for treatments C, CG16, CG8 and SG8 respectively. Measured in August, intakes were 1·8, 20, 2·1 and 2·4 ± O·33kg DM 100kg LW^?1 d^?1 respectively. Severe spring grazing led to increased milk yield and reduced milk fat content from summer-calving cows fed 5·2 kg d^?1 of a proprietary concentrate. Average milk yields for the eleven experimental cows on each treatment were 24·3, 23·4, 26·2 and 29·0 ± 1·20 kgd^?1 (P < 0·01) for C, CG16, CG8 and SG8, and average milk fat contents were 45·4. 42·4, 43·9 and 40·9 ± 1·02gkg^?1 (P<0·05) respectively. The results suggest that severe grazing of swards in early season could improve herbage intake and milk yield of summer-calving cows in mid- and late season. The most favourable spring treatment in this respect was severe grazing by sheep. However, this advantage could be negated in midseason by lax grazing at that time.     

Effects of grass pasture and concentrate-based feeding systems for spring-calving dairy cows in early spring on performance during lactation

The effect of offering a total mixed ration of silage and concentrate (proportionately 0·44 silage) system [indoor feeding system (IF)] was compared with grazing at a high daily herbage allowance with a low level of concentrate supplementation [early grazing system (EG)] in early spring on the performance of spring‐calving dairy cows in Ireland. Sixty‐four spring‐calving Holstein–Friesian dairy cows (mean calving date, 2 February) were allocated to one of two systems between 16 February and 4 April 2004. An equal number of primiparous and multiparous cows were assigned to each system. The dairy cows on the IF system were housed for a 7‐week period and offered a diet of 10·9 kg DM cow^?1 d^?1 (s.d. 2·3) of concentrate, the remainder of the diet was 8·6 kg DM cow^?1 d^?1 (s.d. 1·9) of grass silage. The dairy cows on the EG system were offered a mean daily herbage allowance of 15·1 kg DM cow^?1 d^?1 (s.d. 3·7) and were supplemented with 3·0 kg DM cow^?1 d^?1 (s.d. 1·0) of concentrate. There was no difference in milk yield between the two systems but the cows in the EG system had a higher milk protein concentration (2·9 g kg^?1) and a higher milk protein yield than in the IF system. Milk fat concentration was higher for cows in the IF than EG system (3·0 g kg^?1). There was no difference in total daily dry‐matter intake between the systems, measured in week 6 of the study. Mean live weight of the cows in the IF system was greater than in the EG system. The results of the study suggest that a slightly greater performance can be achieved by a system offering a high daily herbage allowance to spring‐calving dairy cows in early lactation compared with a system offering a total mixed ration containing a high proportion of concentrate with grass silage.     

Dairy cow performance and labour inputs associated with two silage feeding systems

Two experiments were conducted to examine the effect of two winter feeding systems on the performance of dairy cows in early lactation. Experiments 1 (144 d duration) and 2 (146 d duration) involved sixty‐four (primiparous) and eighty‐six (primiparous and multiparous) Holstein Friesian dairy cows respectively. Rations offered comprised grass silage, maize silage [0·26–0·29 of forage dry matter (DM)] and concentrates (10–12 kg d^?1). With the complete diet (treatment CD), the forage and concentrate components were mixed using a complete diet mixer wagon, and offered daily in the form of a ‘complete diet’. With the easy feed (treatment EF), the dairy cows were offered the forage component of the ration twice weekly in whole blocks, in quantities sufficient for the following 3‐ or 4‐d period, while the concentrate component of the diet was offered via electronic out‐of‐parlour feeding stations. Total DM intakes were similar, namely 17·6 and 17·0 kg d^?1 (Experiment 1) and 18·7 and 18·5 kg d^?1 (Experiment 2), for treatments CD and EF respectively. Feeding system had no significant effect on milk yield, milk fat or milk protein content, or on end of study indices of body tissue reserves in either experiment (P > 0·05). Similarly, feeding system had no significant effect on the digestibility of the ration measured in Experiment 2 (P > 0·05). Feeding times associated with each component of the two feeding systems were measured, and these were then used to calculate total feeding time for a 97‐cow dairy herd. Calculated feeding times for this herd were 209·3 and 156·0 min week^?1 for treatments CD and EF respectively.     

The use of n-alkanes to estimate herbage intake and diet composition by dairy cows offered a perennial ryegrass/white clover mixture

The n-alkane technique for estimating herbage intake and diet selection in dairy cows fed perennial ryegrass (Lolium perenne)/white clover (Trifolium repens) herbages was evaluated. Pairs of animals were offered either 8, 10, 12 or 14 kg dry matter (DM) d^?1 of herbage alone or with 2 kg DM d^?1 of barley. Fresh herbage was cut daily from a perennial ryegrass/white clover sward and the appropriate amount was fed in four feeds during the day. Individual intakes and the white clover proportion of the diet were estimated during a 12-d period using the n-alkane technique. Animals were dosed twice daily with paper pellets containing dotriacontane (C₃₂-alkane). Faecal grab samples were collected after the morning and afternoon milking. Three least-squares optimization methods were compared in calculating the white clover proportion in the diet; then, total DM intake was calculated. The different least-squares optimization methods gave similar predictions of the white clover content of the forage consumed. No significant (P < 0.05) effects of sampling routine, concentrate (barley) fed or interactions between the two were detected with respect to the difference between calculated and actual intake, the difference as a proportion of the total intake and estimated white clover content of the diet. The difference between the calculated and actual intake ranged from 139 to 366 g DM d ^?1, which resulted in a proportional difference ranging from 0.004 to 0.02 depending on sampling routine. The actual white clover content of the herbage mixture fed was 0.42 ± 0.008, whereas the estimated white clover content ranged from 0.41 ± 0.006 to 0.43 ± 0.008. The results suggest that accurate herbage intake estimates can be achieved in dairy cows grazing perennial ryegrass/white clover swards if representative samples from herbage consumed can be collected.     

Effects of two mixtures of perennial ryegrass cultivars with contrasting heading dates, and differing in spring-grazing frequency and silage harvest date, on characteristics of silage from first-cut swards

This experiment quantified the effects of: (i) heading date of perennial ryegrass, (ii) grazing frequency in spring and (iii) date of silage harvest, on the ensilability of herbages harvested for silage, and on the conservation and estimated nutritive value of the resultant silages. Replicated field plots with two perennial ryegrass mixtures (intermediate‐ and late‐heading cultivars) were subjected to three spring‐grazing regimes (no grazing, grazing in late March and grazing in both late March and late April) and were harvested on four first‐cut harvest dates between 20 May and 21 June. Herbage from each of the four replicates of these 24 treatments was precision‐chopped and ensiled unwilted and with no additive in laboratory silos. Herbage from the sward with the intermediate‐heading cultivar had a higher (P < 0·001) dry‐matter (DM) content and buffering capacity than that from the late‐heading cultivar, whereas water‐soluble carbohydrate concentrations increased (P < 0·001) with more frequent grazing in spring. Later harvesting enhanced herbage ensilability through an increased (P < 0·001) DM content and reduced (P < 0·001) buffering capacity and pH. Fermentation profiles of the silage were not markedly influenced by the cultivar mixture used but grazing in both late March and late April resulted in a more extensive fermentation with the acids produced increasingly dominated by lactic acid. The concentrations of acetic acid, and to a lesser extent, ethanol declined as silage harvest date was delayed. Overall, the relative effects of grass cultivar mixture were smaller than those of spring‐grazing treatment or silage‐harvesting date although on any given harvest date the herbage from the intermediate‐heading cultivar mixture was easier to preserve as silage than herbage from the late‐heading cultivar mixture. Delaying the harvesting of the late‐heading swards by 8 d removed the differences related to growth stage in buffering capacity, pH and DM content.     

The effect of forage type and level of concentrate supplementation on the performance of spring-calving dairy cows in early lactation

Abstract In 1993 and 1994, 40 cows in early lactation in early spring were assigned randomly to four feeding treatments. One group of cows was kept indoors with access to grass silage ad libitum, plus 6 kg of concentrate daily. The other three groups had access to grass pasture (5–6 h per day in 1993 and 11–12 h per day in 1994) plus grass silage similar to that fed to the previous group while indoors plus 6, 4 or 2 kg of concentrate daily. The average daily allocations of herbage (> 3·5 cm) were 8·5 and 14·0 kg DM cow^?1 day^?1 in 1993 and 1994 respectively. The treatments were applied for 8 weeks (26 February to 23 April) in 1993, and 7 weeks (11 March to 29 April) in 1994. Cows with access to pasture had lower (P < 0·001) silage dry‐matter (DM) intakes and higher (P < 0·001) total forage DM intakes in both years than those kept indoors. This resulted in significantly higher yields of milk, fat, protein and lactose. Similarly, milk protein concentration was higher (P < 0·05 in 1993; P < 0·001 in 1994). There was a significant linear increase in total DM intake in both years with increased concentrate supplementation. In 1993, there was a linear increase in milk (P < 0·01), fat (P < 0·01), protein (P < 0·001) and lactose (P < 0·01) yields with increased concentrate supplementation. In 1994, only milk protein yield (P < 0·05) was increased. Concentrate supplementation had no effect on milk composition or liveweight change. Cows with access to grazed grass had higher liveweight gains (P < 0·05) than those kept indoors in both years. In 1993, increasing the energy intake increased the processing qualities of the milk produced. The results showed that access to grass pasture resulted in higher milk production, in reduced silage requirement and in reduced level of concentrate supplementation required for a given level of milk production with spring‐calving cows in early lactation compared with those kept indoors.     

A short-term comparison of the performance of four grassland-based systems of milk production for autumn-calving dairy cows

Eighty winter‐calving dairy cows of mixed parity were managed in four grassland‐based systems of milk production (F‐F, F‐C, C‐F and C‐C) over a full lactation (year 1) and during the winter period of the subsequent lactation (year 2). During the winter periods cows on systems F‐F and F‐C were offered silages of high feeding value, supplemented with 6·0 kg d^?1 of concentrate [crude protein (CP), 307 g kg^?1 dry matter (DM)] through an out‐of‐parlour feeding system, while cows on systems C‐F and C‐C were offered silages of medium feeding value, supplemented with c. 12·8 kg d^?1 of concentrate (CP, 204 g kg^?1 DM), in the form of a complete diet. After 25 February in year 1, cows on systems F‐F and C‐F were given access to grazing for periods of increasing duration, achieving full turnout on 17 April. Thereafter, until 21 October, these cows were offered a high daily allowance of herbage within a flexible grazing system (23·0 kg DM per cow, measured above a height of 4·0 cm), supplemented with 0·5 kg d^?1 of a ‘high magnesium’ concentrate. Cows on systems F‐C and C‐C (year 1) commenced grazing on 1 April, achieving full turnout on 17 April. Thereafter, until 20 October, these cows were managed on a restricted allowance of herbage in a rotational paddock grazing system, with concentrates (average allocation, 3·9 kg d^?1) being offered according to yield. In year 2, cows on systems F‐F and C‐F were given access to grazing for periods of increasing duration, from 11 March to 8 April, at which point the study was terminated. With systems F‐F, F‐C, C‐F and C‐C, mean feed inputs and milk outputs (per animal) during year 1 of the study were as follows: total concentrate DM intakes [881, 1272, 1729 and 2171 kg (s.e.m. 96·1)]; total silage DM intakes [1722, 1713, 1047 and 1154 kg (s.e.m. 70·7)], total grass DM intakes (3245, 2479, 3057 and 2481 kg) and total milk outputs [7541, 7527, 7459 and 7825 kg (s.e.m. 305·8)] respectively. Stocking rates associated with each of the four systems were 2·2, 2·5, 2·6 and 2·9 cows per hectare respectively. Performance of dairy cows on the systems during the winter of year 2 was similar to that recorded during year 1. The results of this study indicate that similar levels of milk output, DM intakes, tissue changes and plasma metabolite profiles can be achieved from grassland‐based systems involving very different combinations of grass silage, grazed grass and concentrate feeds.     

The effect of initial spring grazing date and subsequent stocking rate on the grazing management, grass dry matter intake and milk production of dairy cows in summer

The objective of this study was to investigate the effects of an early (February; F) or delayed (April; A) primary spring grazing date and two stocking rates, high (H) and medium (M), on the grazing management, dry matter (DM) intake of grass herbage and milk production of spring‐calving dairy cows grazing a perennial ryegrass sward in the subsequent summer. Sixty‐four Holstein‐Friesian dairy cows (mean of 58 d in milk) were assigned to one of four grazing treatments (n = 16) which were imposed from 12 April to 3 July 2004. Cows on the early spring‐grazing treatment were grazed at 5·5 cows ha^?1 (treatment FH) and 4·5 cows ha^?1 (treatment FM) while cows on the late‐grazing treatment were grazed at 6·4 cows ha^?1 (treatment AH) and 5·5 cows ha^?1 (treatment AM). The organic matter digestibility and crude protein concentration of the grass herbage were higher on the early‐grazing treatment than on the late‐grazing treatment. The cows on the FM treatment had significantly (P < 0·001) higher milk (24·5 kg), solids‐corrected milk (22·5 kg), fat (P < 0·01, 918 g) and protein (831 g) yields than the other three treatments. Cows on the FM treatment had a higher (P < 0·001) DM intake of grass herbage by 2·3 kg DM per cow per day than cows on the AH treatment, which had a DM intake significantly lower than all other treatments (15·2 kg DM per cow per day). The results of the present study showed that grazing in early spring has a positive effect on herbage quality in subsequent grazing rotations. The study also concluded that early spring‐grazed swards stocked at a medium stocking rate (4·5 cows ha^?1; FM) resulted in the highest DM intake of grass herbage and milk production.     

In vitro rumen methane output of perennial ryegrass varieties and perennial grass species harvested throughout the growing season

The selection and feeding of perennial ryegrass (Lolium perenne L.) varieties (PRV) or perennial grass species (PGS) may affect enteric methane (CH₄) output because of changes in the fermentation dynamics in the rumen as a result of differences in herbage chemical composition. The objective of this study was to determine the effects of PRV and PGS harvested throughout the growing season on herbage chemical composition, and in vitro rumen fermentation variables and CH₄ output per unit of feed using a batch culture technique. Seven PRV (Experiment 1: Alto, Arrow, Bealey, Dunluce, Greengold, Malone, Tyrella) and six perennial grasses [Experiment 2: perennial ryegrass (Navan), perennial ryegrass (Portstewart), cocksfoot, meadow fescue, tall fescue, timothy; defined as PGS], managed under a simulated grazing regime, were incubated for 24 h with buffered rumen fluid in two separate experiments. The CH₄ output per unit of feed dry‐matter (DM) incubated was not affected (P > 0·05) by PRV (range of mean values across PRV of 23·9–25·3 (SEM 0·41) mL g^?1 DM) or by PGS (25·6–26·6 (SEM 0·37) mL g^?1 DM). The CH₄ output per unit feed DM disappearing during the in vitro rumen incubation was not affected by PRV (33·9–35·1 (SEM 0·70) mL g^?1 DM), and although there was an overall PGS effect (P < 0·05; 37·2–40·3 (SEM 0·71) mL g^?1 DM), none of the paired contrasts between PGS were significant when analysed using Tukey adjusted comparisons. This outcome reflected either small‐scale or a lack of treatment effects on individual herbage chemical composition (e.g. 454–483 g NDF kg^?1 DM, 215–224 g CP kg^?1 DM and 94–122 g water‐soluble carbohydrate (WSC) kg^?1 DM across PRV; 452–506 g NDF kg^?1 DM, 208–243 g CP kg^?1 DM and 73–131 g WSC kg^?1 DM across PGS) and in vitro rumen fermentation variables. Hence, these results provide no encouragement that choices among the grasses examined, produced within the management regimes operated, would reduce enteric CH₄ output per unit of feed in vivo. However, the technique utilized did not take account of animal × PRV or PGS interactions, such as potential differences in intake between animals, that may occur under farm conditions.