Evaluation of tiamulin for treatment of mycoplasmal pneumonia in swine

During 3 trials, using affected pigs of various ages, tiamulin was evaluated for treatment of experimentally induced mycoplasmal pneumonia. Pneumonia was induced in respiratory tract disease-free swine by intratracheal inoculation of a lung homogenate containing Mycoplasma hyopneumoniae. Eleven days after inoculation, when more than 20% of pigs were coughing, pigs were allotted to 3 or 4 groups (n = 8 pigs each) and were given regimens of no medication or 60 mg, 120 mg, or 180 mg of tiamulin/L of drinking water for 10 days. Twenty-one days after cessation of medication, pigs were euthanatized and then were necropsied. Results obtained from the 3 trials did not indicate significant difference among treatment groups in severity of macroscopic or microscopic lesions induced by M hyopneumoniae or in detection of M hyopneumoniae by use of immunofluorescent technique. Clinical evaluations, daily gain, and feed efficiency did not differ significantly among treatment groups. In this study, tiamulin administration did not have beneficial effects in swine with mycoplasmal pneumonia.     

Evaluation of criteria for the postmortem diagnosis of mycoplasmal pneumonia of swine.

Ten swine from each of five herds believed to be affected with mycoplasmal pneumonia of swine and ten swine from each of five herds believed to be mycoplasmal pneumonia-free were selected for postmortem study. Lungs from the 100 swine were examined; grossly and microscopically for lesions typical of mycoplasmal pneumonia of swine and culturally and by an indirect immunofluorescent procedure for the presence of Mycoplasma hyopneumoniae. Nineteen of the lungs had both gross and microscopic lesions typical of mycoplasmal pneumonia of swine and 13 (68%) of these were infected, i.e. were culturally and/or indirect immunofluorescent positive. Absence of gross lesions did not prove freedom from mycoplasmal pneumonia, 14 of 73 (19%) grossly normal lungs were found to be infected with M. hyopneumoniae. Comparison of the indirect immunofluorescent and cultural examination, as methods of diagnosing mycoplasma pneumonia, revealed that neither procedure alone was reliable in the case of negative results. Ten lungs were indirect immunofluorescent negative and culturally positive and seven were culturally negative and indirect immunofluorescent positive (11 lungs were positive by both procedures). It was concluded that a definitive diagnosis of mycoplasmal pneumonia of swine requires that M. hyopneumoniae be visualized in indirect immunofluorescent stained lung sections or that it be recovered culturally.     

Evaluation of diagnostic procedures for detection of mycoplasmal pneumonia of swine.

The complement-fixation test (CFT), a latex-agglutination test (LAT), and Mycoplasma hyopneumoniae isolation procedures were compared with gross and microscopic lung evaluations for the routine diagnosis of mycoplasmal pneumonia (MP) of swine. The difficulty and variability of M hyopneumoniae isolation rendered this technique unacceptable. In market-weight swine, the finding of typical gross lesions was correlated with CFT results (P less than 0.005). The MP status of a herd could not be determined by use of these techniques in 4- to 9-week-old pigs. No technique could be used alone to establish a diagnosis. Gross and microscopic examination of all slaughter specimens, CFT, and clinical evaluation of the herd, used in combination, were the most useful means of determining the MP status of the herd.     

猪地方流行性肺炎综合控制措施

猪地方流行性肺炎,又称为猪支原体性肺炎,俗称猪气喘病,是由猪肺炎支原体所引起的一种慢性接触性呼吸道传染病,临床上主要以咳嗽和气喘为特征性症状,发病率高,死亡率低,病猪生长发育迟缓,饲料转化率低,生猪抵抗力下降,极易并发其他呼吸道疾病。近年来,本病的发生在当地呈上升态势,是养猪生产中极为顽固的呼吸道传染病。一、病原体与流行特点:1、猪地方流行性肺炎的病原体是由猪肺炎支原引起,该病原体是一种无细胞壁的多形性微生物。存在于病猪的呼吸器官内,随咳嗽、喷嚏等分泌物排出体外,污染猪舍、饲具和用具。通过空气、接触、环境污染等…     

An indirect hemagglutination test for the diagnosis of Eperythrozoon suis infection in swine.

An indirect hemagglutination test was developed to detect naturally occurring eperythrozoonosis in swine and to follow experimentally induced infections serologically. The antigen used was prepared from the plasma of acutely infected, splenectomized pigs. The test was specific and reliable in detecting swine latently infected with the disease.     

猪支原体肺炎的综合防治

猪支原体肺炎(MPS)又称猪喘气病,是由猪肺炎支原体引起的一种慢性呼吸道病.猪肺炎支原体属于支原体科支原体属,存在于病猪的呼吸道(咽喉、气管、肺组织)、肺门淋巴结和纵隔淋巴结中,具有多形性.猪肺炎支原体带菌猪是本病主要传染源.健康猪群常因引进病猪而使本病暴发.     

泰乐松注射液治疗猪喘气病试验效果观察

猪喘气病(mycoplasmal pneumonia of swine,MPS)又称猪地方流行性肺炎,是猪的慢性呼吸道疾病,其病原为猪肺炎支原体。主要症状为咳嗽和气喘,病变特征是肺的尖叶、心叶、中间叶和膈叶前缘呈“肉样”或“虾肉样”实变。本病一年四季均可发生,     

中药炎康注射液治疗猪气喘病的试验观察

猪气喘病又叫猪支原体肺炎,我国的发病率也较高(见表1).患猪长期生长发育不良,饲料转化率低,给养猪业发展带来了严重的经济损失.据有关资料表明,由于支原体的感染,育肥猪每增重1 kg,比健康猪多消耗0.1～0.2 kg饲料.     

猪气喘病的治疗与预防控制关键点

猪呼吸道疾病中以猪气喘病最为常见，也是最难根除治愈的疾病之一，因其特有的致病机理所致，该病给全世界养猪业造成的损失巨大。根除受感染的猪场中的病原是可行的，但实际不容易做到，所以预防该病的临床综合症的发生和减少经济损失是防制猪气喘病最根本之原则。本文主要从中西医的角度来描述治疗猪气喘病的方法和规模化猪场应控制的几个关键点。     

Failure of lungworm-larvae-infected earthworms to transmit mycoplasmal pneumonia to swine

Two herds of swine, believed to be free from mycoplasmal pneumonia of swine (MPS) based upon negative Mycoplasma hyopneumoniae microtiter complement fixation test (CFT) results, subsequently exhibited clinical signs of MPS.Lungworms (Metastrongylus spp.) were common to both herds. The possibility that lungworms could serve as a reservoir for M. hyopneumoniae was investigated. Lungworm-larvae-infected earthworms, collected from each of the farms, were fed to swine free of lungworms and MPS. This procedure resulted in lungworm infection in the recipient pigs, but failed to produce pneumonic lesions or CFT antibody titers against MPS.     

Comparison of manually performed Microtiter plate and semiautomated (cuvette) indirect enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine

Standardized enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine were developed, using the traditional Microtiter plate method and a method based on semiautomated processor-analyzer instrumentation. The results of the 2 procedures were not significantly different when the titers of positive and negative anti-Mycoplasma hyopneumoniae standard reference sera were determined in triplicate in 3 separate experiments. Although both methods yielded reproducible results, the instrument-assisted enzyme-linked immunosorbent assay would be preferable if large numbers of samples are to be tested.     

四种方法治疗猪气喘病的疗效对比试验

为观察4种治疗方法治疗猪喘气病的疗效并进行对比,选出较好的治疗方案,以提高猪喘气病的治愈率,本试验选某猪场确诊为猪喘气病的病猪60头,将患猪随机分4组,分别采用麻杏石甘汤加味配合西药穴位注射、单用麻杏石甘汤加味、单用西药及针灸的治疗方法。结果显示,麻杏石甘汤加味配合西药穴位注射组的治愈率为93.33%;麻杏石甘汤加味组治愈率为73.33%;西药组治愈率为80.00%;针灸组治愈率为46.67%。试验证明,采用麻杏石甘汤加味配合西药穴位注射治疗猪喘气病的疗效高于其他3种方法,为临床治疗该病提供了确实可行的治疗方法。     

An indirect hemagglutination test for the detection of antibodies to Clostridium chauvoei

An indirect hemagglutination (IHA) test using sonicated extract as the antigen was developed for the detection of antibodies to Clostridium chauvoei. This antigen can be adsorbed onto glutaraldehyde-fixed sheep red blood cells treated with tannic acid and can be destroyed by trypsin and heat treatment. It corresponded well with the flagella of the organism, when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the gel diffusion test. No serological cross-reactivity was found in the IHA test when the antigen was tested against 4 species of clostridial antibodies. Our results suggest that the IHA test mainly detects antibodies against the flagella of C. chauvoei.