Water- and plant-mediated responses of soil respiration to topography,fire, and nitrogen fertilization in a semiarid grassland in northern China

Soil respiration is one of the major carbon (C) fluxes between terrestrial ecosystems and the atmosphere and plays an important role in regulating the responses of ecosystem and global C cycling to natural and anthropogenic perturbations. A field experiment was conducted between April 2005 and October 2006 in a semiarid grassland in northern China to examine effects of topography, fire, nitrogen (N) fertilization, and their potential interactions on soil respiration. Mean soil respiration was 6.0% higher in the lower than upper slope over the 2 growing seasons. Annual burning in early spring caused constant increases in soil respiration (23.8%) over the two growing seasons. In addition, fire effects on soil respiration varied with both season and topographic position. Soil respiration in the fertilized plots was 11.4% greater than that in the unfertilized plots. Water- and plant-mediation could be primarily responsible for the changes in soil respiration with topography and after fire whereas the positive responses of soil respiration to N fertilization were attributable to stimulated plant growth, root activity and respiration. The different mechanisms by which topography, fire, and N fertilization influence soil respiration identified in this study will facilitate the simulation and projection of ecosystem C cycling in the semiarid grassland in northern China.     

长期施氮肥对黄棕壤微生物生物性状的影响及其调控因素

基于黄棕壤小麦-甘薯轮作的长期定位试验,探究不同施氮处理土壤微生物生物量碳(MBC)、氮(MBN)含量和酶活性的变化及其潜在调控因子,为科学施氮提高土壤质量和改善土壤生态功能提供依据。试验选取始于2011年4个施氮处理:不施肥(CK)、不施氮肥(PK)、施化学氮肥(NPK)和化学氮肥配施有机肥(NPKM),调查两季作物收获后土壤MBC和MBN含量、酶活性及微生物碳氮利用效率的变化,并通过冗余分析(RDA)和结构方程模型(SEM)明确调控弱酸性黄棕壤中MBC和MBN变化的潜在因素。小麦和甘薯两季的结果表明:施氮肥降低了土壤MBC、MBN含量和蔗糖酶(SSC)、脲酶(SUE)活性,与NPK处理相比, NPKM处理增加了MBC、MBN含量和SSC、SUE活性。长期施用氮肥提高了土壤有机碳(SOC)和土壤养分[全氮(TN)和矿质态氮(MN)]含量,但施氮肥显著降低土壤p H以及微生物的碳氮利用效率。与小麦季相比,甘薯季土壤SOC和MN含量有所下降,而MBN含量和SSC活性有所升高。RDA和SEM结果表明,氮肥的施用强化了MBC与MBN、SSC与MBC以及SUE与MBC之间的关联性;不同施氮处理下土壤p H、有机碳、氮含量以及微生物的碳氮利用效率的变化直接或间接地影响土壤MBC、MBN含量和SSC、SUE活性,其中p H是调控土壤MBC变化的直接因素,而土壤SSC和SUE活性与MBC、MBN含量相互影响。长期施用氮肥降低了黄棕壤MBC、MBN含量和酶活性,化学氮肥配施有机肥有利于缓解生物性状的下降,土壤p H是影响MBC变化的主要因素,小麦-甘薯轮作中土壤微生物强烈的碳代谢过程利于增加MBN。     

不同施肥模式对设施菜田土壤微生物量碳、氮的影响

【目的】 本文利用天津日光温室蔬菜不同施肥模式定位试验,研究了不同施肥模式对设施菜田土壤微生物量碳、氮含量的影响,为设施蔬菜高效施肥和菜田土壤可持续利用提供依据。 【方法】 调查在第 9 茬蔬菜 (秋冬茬芹菜) 和第 10 茬蔬菜 (春茬番茄) 进行。定位试验设 8 个处理,分别为:1) 不施氮;2) 全部施用化肥氮 (4/4CN);3) 3/4 化肥氮 + 1/4 猪粪氮 (3/4CN + 1/4PN);4) 2/4 化肥氮 + 2/4 猪粪氮 (2/4CN + 2/4PN);5) 1/4 化肥氮 + 3/4 猪粪氮( 1/4CN + 3/4PN);6) 2/4 化肥氮 + 1/4 猪粪氮 + 1/4 秸秆氮 (2/4CN + 1/4PN + 1/4SN);7) 2/4 化肥氮 + 2/4 秸秆氮 (2/4CN + 2/4SN);8) 农民习惯施肥 (CF),除不施氮肥和农民习惯施肥外,其余处理为等氮磷钾处理。在不同生育时期,采 0—20 cm 土壤样品,测定土壤微生物量碳、氮含量,并分析其与蔬菜产量之间的关系。 【结果】 两茬蔬菜不同施肥模式土壤微生物量碳、氮含量总体上均随生育期的推进呈先增后降的趋势。芹菜季较高土壤微生物量碳含量出现在定植后 90 d,土壤微生物量氮较高含量出现在定植后 60 d;番茄季分别出现在定植后 20～80 d 和 60 d。芹菜季 5 个有机无机肥料配施模式土壤微生物量碳、氮含量分别在 185.0～514.6 和 34.3～79.1 mg/kg 之间,较化肥(4/4CN)模式平均分别增加 15.1%～81.7% 和 24.5%～100.0%,其中以配施秸秆模式土壤微生物量碳、氮含量相对较高,平均分别增加 62.0%～81.7% 和 81.1%～100.0%;番茄季 5 个有机无机肥料配施模式土壤微生物量碳、氮含量分别在 120.7～338.0 和 25.5～68.8 mg/kg 之间,较 4/4CN 模式平均分别增加 16.9%～86.9% 和 12.2%～109.3%,又以配施秸秆模式土壤微生物量碳、氮含量最高,平均分别增加 61.4%～86.9% 和 78.2%～109.3%。两季蔬菜不同生育期土壤微生物量碳、氮含量与当季蔬菜产量和定位试验开始以来蔬菜总产量之间均呈极显著正相关关系。 【结论】 同等养分投入量下,有机无机肥料配合施用提高土壤微生物量碳、氮的效果显著好于单施化肥,又以化肥配施秸秆效果更佳;土壤微生物量碳、氮含量与设施蔬菜产量之间呈极显著正相关关系。证明有机无机肥配施,特别是配施一定量的秸秆可有效提高土壤微生物量碳、氮含量,维持较高的菜田土壤肥力,有利于设施蔬菜的可持续和高效生产。      

有机无机肥配施对盐渍化土壤微生物量和呼吸的影响

微生物可以通过摄入能量合成有机渗透压物质来实现对盐度的适应,然而,不同程度盐渍土微生物对能量的需求可能会发生改变。因此,该研究于2018－2019年开展田间定位试验,选取河套灌区轻度盐渍土S1（电导率为0.46 dS/m）及中度盐渍土S2（电导率为1.07 dS/m）为研究对象,设置了6个处理,包括不施氮（CK）,单施无机氮（U1）以及分别用有机氮（U3O1、U1O1、U1O3和O1）替代25%、50%、75%和100%的无机氮,监测了土壤微生物量碳氮及土壤呼吸在第二个生长季的动态状况。结果表明：土壤盐渍化程度增加会导致土壤微生物量及微生物活性下降,S2土壤较S1土壤微生物量碳下降12.01%～68.81%,土壤微生物量氮下降14.31%～58.58%,土壤呼吸速率下降11.75%～54.71%。不同盐分条件下,适当的有机肥施入比例可以显著提高土壤微生物量及微生物活性,S1和S2盐渍土分别以U1O1及O1处理较优,土壤微生物量碳、微生物量氮、土壤呼吸分别较U1处理提高48.44%、42.50%、31.74%,68.07%、48.99%、45.19%。相关性分析表明,土壤呼吸速率与土壤微生物量碳氮呈极显著正相关（P＜0.01）,土壤温度、土壤矿质氮与土壤微生物量碳氮、土壤呼吸速率呈显著正相关（P＜0.05）。从玉米产量及改善土壤微生物生存环境角度,得到该地区适宜的施肥模式为,轻度盐渍土：有机氮替代50%无机氮;中度盐渍土：有机氮替代100%无机氮。     

The impact of mulch type on soil organic carbon and nitrogen pools in a sloping site

Three mulch treatments were tested for their ability to control erosion on a sloping site. Additionally, the choice of mulch can also enhance revegetation success and improve soil organic matter input. This study aimed to investigate the effects of three mulching treatments, hydro-seeding, granite mulch and forest mulch, on soil C and N pools at different positions on highly erodible slope with approximately 30 % gradient. Soil moisture, total C (TC), total N (TN), hot water-extractable organic C (HWEOC), hot water-extractable total N (HWETN), microbial biomass C and N (MBC and MBN), inorganic N and potentially mineralisable N were measured. All variables were significantly higher in soils amended with forest mulch than those with hydro-seeding and granite mulch, for the same slope positions. Soil moisture was significantly higher in the lower slope position than middle and upper slope positions in hydro-seeding and granite mulch treatments, whereas no slope effect was observed on soil moisture under the forest mulch application. In the forest mulch treatment, the upper slope position had higher soil TC, TN, HWEOC, HWETN, MBC, MBN, NO₃ ^?-N and total inorganic N than the middle and lower slope positions. Five years following mulch application, forest mulch still exerted a significant influence on soil fertility compared to the other treatments and the influence on soil moisture suggests that this treatment would be the most effective in the control of water-driven soil erosion on this steep site.     

Effect of land management and soil texture on seasonal variations in soil microbial biomass in dry tropical agroecosystems in Tanzania

Soil microbes are an essential component of most terrestrial ecosystems; as decomposers they are responsible for regulating nutrient dynamics, and they also serve as a highly labile nutrient pool. Here, we evaluated seasonal variations in microbial biomass carbon (MBC) and nitrogen (MBN) as well as microbial activity (as qCO₂) for 16 months with respect to several factors relating to soil moisture and nutrients under different land management practices (plant residue application, fertilizer application) in both clayey (38% clay) and sandy (4% clay) croplands in Tanzania. We observed that MBC and MBN tended to decrease during the rainy season whereas they tended to increase and remain at high levels during the dry season in all treatment plots at both of our test sites, although soil moisture did not correlate with MBC or MBN. qCO₂ correlated with soil moisture in all treatment plots at both sites, and hence soil microbes act as decomposers mainly during the rainy season. Although the effect of seasonal variation of soil moisture on the dynamics of MBC, MBN, and qCO₂ was certainly greater than that attributable to plant residue application, fertilizer application, or soil texture, plant residue application early in the rainy season clearly increased MBC and MBN in both clayey and sandy soils. This suggests that plant residue application can help to not only counter the N loss caused by leaching but also synchronize crop N uptake and N release from soil microbes by utilizing these microbes as an ephemeral nutrient pool during the early crop growth period. We also found substantially large seasonal variations in MBC and MBN, continuously high qCO₂, and rapid turnover of soil microbes in sandy soil compared to clayey soil. Taken together, our results indicate that soil microbes, acting as both a nutrient pool and decomposers, have a more substantial impact on tropical sandy soil than on clayey soil.     

有机物料输入稻田提高土壤微生物碳氮及可溶性有机碳氮

土壤微生物量碳、氮和可溶性有机碳、氮是土壤碳、氮库中最活跃的组分,是反应土壤被干扰程度的重要灵敏性指标,通过设置相同有机碳施用量下不同有机物料处理的田间试验,研究了有机物料添加下土壤微生物量碳（soil microbial biomass carbon,MBC）、氮（soil microbial biomass nitrogen,MBN）和可溶性有机碳（dissolved organic carbon,DOC）、氮（dissolved organic nitrogen,DON）的变化特征及相互关系。结果表明化肥和生物碳、玉米秸秆、鲜牛粪或松针配施下土壤微生物量碳、氮和可溶性有机碳、氮显著大于不施肥处理（no fertilization,CK）和单施化肥处理,分别比不施肥处理和单施化肥平均高23.52%和12.66%（MBC）、42.68%和24.02%（MBN）、14.70%和9.99%（DOC）、22.32%和21.79%（DON）。化肥和有机物料配施处理中,化肥+鲜牛粪处理的微生物量碳、氮和可溶性有机碳、氮最高,比CK高26.20%（MBC）、49.54%（MBN）、19.29%（DOC）和32.81%（DON）,其次是化肥+生物碳或化肥+玉米秸秆处理,而化肥+松针处理最低。土壤可溶性有机碳质量分数（308.87 mg/kg）小于微生物量碳（474.71 mg/kg）,而可溶性有机氮质量分数（53.07 mg/kg）要大于微生物量氮（34.79 mg/kg）。与不施肥处理相比,化肥和有机物料配施显著降低MBC/MBN和DOC/DON,降低率分别为24.57%和7.71%。MBC和DOC、MBN和DON随着土壤有机碳（soil organic carbon,SOC）、全氮（total nitrogen,TN）的增加呈显著线性增加。MBC、MBN、DOC、DON、DOC+MBC和DON+MBN之间呈极显著正相关（P<0.01）。从相关程度看,DOC+MBC和DON+MBN较MBC、DOC、MBN、DON更能反映土壤中活性有机碳和氮库的变化,成为评价土壤肥力及质量的更有效指标。结果可为提高洱海流域农田土壤肥力,增强土壤固氮效果,减少土壤中氮素流失,保护洱海水质安全提供科学依据。     

Soil microbial activity and biomass is stimulated by leguminous cover crops

The leguminous cover crops Atylosia scarabaeoides (L.) Benth., Centrosema pubescens Benth., and Pueraria phaseoloides (Roxb.) Benth., were grown in the interspaces of a 19 y–old coconut plantation and incorporated into the soil at the end of the monsoon season every year. At the end of the 12th year, soils from different depths were collected and analyzed for various microbial indices and their interrelationships. The objectives were to assess the effects of long‐term cover cropping on microbial biomass and microbial‐community structure successively down the soil profile. In general, total N (TN), organic C (OC), inorganic N, extractable P, and the levels of biological substrates viz., dissolved organic C (DOC) and N (DON), labile organic N (LON), and light‐fraction organic matter (LFOM) C and N decreased with depth at all the sites. Among sites, the cover‐cropped (CC) sites possessed significantly greater levels of TN, OC, DOC, DON, and LON compared to the control. Consequently, microbial biomass C (MBC), N (MBN), and P (MBP), CO₂ evolution, and ATP levels, in general, decreased with depth at all sites and were also significantly higher in the CC sites. Among the ratios of various microbial indices, the ratio of MBC to OC and metabolic quotient (qCO₂) declined with depth. Higher MBC‐to‐OC ratios and large qCO₂ levels in the surface soils could be ascribed to greater levels of readily degradable C content and indicated short turnover times of the microbial biomass. In contrast, the ratios of MBC to MBN and MBC to MBP increased with depth due to low N/P availability and relatively higher C availability in the subsoils. Cover cropping tended to enhance the ratios of MBC to OC, MBC to MBN, MBC to MBP, and ergosterol to MBC and decreased the ATP‐to‐MBC ratio at all depths. The relatively lower ATP‐to‐MBC ratios in the CC site, especially in the subsoil indicated microbial‐community structure possibly dominated by fungi. By converting the ergosterol content to fungal biomass, it was observed that fungi constituted 52%–63% of total biomass C at the CC site, but only 33%–40% of total biomass C at the control site. Overall, the study indicated that leguminous cover crops like P. phaseoloides or A. scarabaeoides significantly enhanced the levels of OC, N and microbial activity in the soils, even down to 50 cm soil depth.     

Nitrogen addition and mowing affect microbial nitrogen transformations in a C4 grassland in northern China

Microbial nitrogen (N) transformations play a key role in regulating N cycling in grassland ecosystems. However, there is still little information on how management of semi‐arid grassland such as mowing and/or N fertilizer application affects microbial activity and N transformations. In a field experiment in northern China, N was added at a rate of 10 g N m^?2 year^?1 as NH₄NO₃ to mown and unmown plots (4 × 4 m²) and in situ rates of net ammonification (R_amm), nitrification (R_nit) and mineralization (R_min) were followed at monthly intervals for the vegetation growth periods in the years 2006–2009. In addition, we also measured soil microbial biomass carbon (MBC) and nitrogen (MBN), microbial respiration (MR) and peak above‐ground biomass in August of each measurement year. Driven by the pronounced inter‐annual variability of rainfall, all the properties investigated varied markedly across years. Nevertheless, we were able to demonstrate that over the 4 years N addition significantly stimulated R_nit, R_min and MBN, on average, by 288, 149 and 11.6%, respectively. However, N addition decreased MBC significantly as well as the ratio of MBC:MBN by, on average, 10 and 23%, respectively, whereas an effect of N addition on MR could not be demonstrated. Mowing decreased MBN, MR and qCO₂ significantly by 9, 28 and 24%, respectively, but no effects were found on microbial net N transformation rates and MBC. N addition and mowing interactively affected R_amm and R_min, and MBN, MBC:MBN. In summary, our results indicate a positive effect of N addition but a negative effect of mowing on microbial N transformation in this C4 grassland in northern China.     

山核桃集约经营过程中土壤微生物量碳氮的变化

[目的]研究不同集约经营历史山核桃林的土壤微生物量碳氮的演变规律,为山核桃林地土壤管理提供科学依据。[方法]在浙江省临安市分别采集并分析了经营历史为5,10,15,20a的山核桃林土壤样品,并与天然混交林(0a)进行比较。[结果]天然混交林改造为山核桃纯林并经集约经营后,林地土壤微生物量碳(MBC)、微生物量氮(MBN)、MBC/MBN,MBC/SOC均表现出先下降而后上升的趋势,经过10a经营后降到最低水平,与0a相比,0—10cm土层MBC,MBN和MBC/SOC分别降低了52.1%,32.0%和31.0%。经营10a的林地土壤MBC/MBN显著低于前期经营林地,而MBN/TN在经营过程中的差异并不显著。[结论]山核桃集约经营后,林地土壤微生物量碳氮含量显著下降。     

Response of soil microbial community and diversity to increasing water salinity and nitrogen fertilization rate in an arid soil

The scarcity of fresh water has forced farmers to use saline water (SW) for irrigation. It is important to understand the response of the soil microbial community and diversity to saline irrigation water. The objective of this study was to determine the effects of irrigation water salinity and nitrogen fertilization rates on soil physicochemical properties, microbial activity, microbial biomass, and microbial functional diversity. The field experiment consisted of a factorial design with three levels of irrigation water salinity (electrical conductivities (ECs) of 0.35, 4.61 or 8.04?dS?m^?1) and two nitrogen rates (0 and 360?kg?N?ha^?1). The results showed that the 4.61 and 8.04?dS?m^?1 treatments both reduced soil microbial biomass C (MBC), microbial biomass N (MBN), basal respiration, total phospholipid fatty acid (PLFA), bacterial PLFA, fungal PLFA, and fungal:bacterial ratios. In contrast, the SW treatments increased the MBC:MBN ratio. Nitrogen fertilization increased soil MBC, MBN, basal respiration, total PLFA, bacterial PLFA, and gram-negative bacterial PLFA. In contrast, N fertilization decreased gram-positive bacterial PLFA, fungal PLFA, and fungal:bacterial ratios. Average well color development, Richness, and Shannon's Index were always lowest in the 8.04?dS?m^?1 treatment. Carbon utilization patterns in the 8.04?dS?m^?1 treatment were different from those in the 0.35?dS?m^?1 treatment. In conclusion, five years of irrigation with brackish or SW reduced the soil microbial biomass, activity, and functional diversity, which may cause the deterioration of soil quality. Thus, the high-salinity water (EC?>?4.61?dS?m^?1) is not appropriate as a single irrigation water resource. Proper N fertilizer input may overcome some of the negative effects of salinity on soil microbial.     

Dynamics of soil labile carbon and nitrogen pools in riparian zone of Wyaralong Dam in Southeast Queensland,Australia

Purpose

Soil labile carbon (C) and nitrogen (N) pools are considered to be sensitive indicators of changes in soil C and N pools. In this study, we examined possible factors affecting spatial and seasonal variations in soil labile C and N pools in the riparian zones in Southeast Queensland, Australia.

Materials and methods

Soil and sediment samples were collected from two sites in the riparian areas. The spatial and seasonal variabilities of soil moisture, hot-water extractable organic C and total N (HWEOC and HWETN), microbial biomass C and N (MBC and MBN), and the relationships among them were examined.

Results and discussion

Soil labile C and N pools decreased along the transects in both soil depths of the two soil types, with the peak or bottom of values detected between upland slope and the riparian zone. Other factors rather than soil moisture were more important in regulating seasonal changes of soil HWEOC and HWETN except the dry-rewetting influence in November 2013. Soil moisture played a significant role in the seasonal variations of MBC and MBN. Soil labile C (HWEOC and MBC) and N (HWETN and MBN) pools at Site 1 (S₁; heavy texture), which were significantly higher than those at Site 2 (S₂; light texture).

Conclusions

Soil moisture would be an important driving factor for the spatial and seasonal distributions of soil labile C and N pools. Our study highlighted the importance of riparian zones as the hot spot of soil C and N dynamics, especially at the onset of rewetting dry soil in subtropical Australia.

     

抛荒土地不同处理及利用方式对喀斯特地区土壤微生物的影响

土壤微生物特性是土壤养分的储存库,土壤养分也影响土壤微生物活性,了解两者的相互作用机制对土地利用与管理提供理论依据,而喀斯特地区不同土地利用方式相关研究较少。基于喀斯特峰丛洼地火烧、刈割、刈割除根、封育、种植玉米、种植桂牧1号杂交象草6种坡面典型的土地利用方式的动态监测样地建设与调查,该文分析了不同土地利用方式下土壤微生物特性,揭示其与土壤养分相互作用机制。结果表明,不同土地利用方式对土壤养分的影响不同,土壤有机质（soil organic matter,SOM）、全氮（total nitrogen,TN）、全磷（total phosphorus,TP）、碱解氮（available nitrogen,AN）等沿封育、火烧、刈割、刈割除根、种植桂牧1号、种植玉米等土地利用方式的转变而减少;不同土地利用方式土壤微生物生物量各不同,土壤微生物量碳（microbial biomass carbon, MBC）：244.98～1 246.89 mg/kg、土壤微生物量氮（microbial biomass nitrogen,MBN）：35.44～274.69 mg/kg、土壤微生物量磷（microbial biomass phosphorus,MBP）：30.88～64.72 mg/kg,其中,种植玉米土壤微生物生物量均最低,其土壤质量退化现象严重;不同土地利用方式土壤微生物种群数量及组成影响不同,种植玉米和桂牧1号杂交象草细菌占绝对优势,而火烧、刈割、刈割除根及封育均以放线菌占绝对优势,真菌的比例很少;不同土地利用方式土壤微生物特性与土壤养分之间相互作用关系不同：火烧的土壤TP和MBP、全钾（total potassium,TK）与MBC、TN与放线菌作用最强且均呈正相关,刈割的土壤TN与MBC呈正相关,刈割除根的速效磷（available phosphorus,AP）与MBN正相关,封育的pH值与MBC、真菌负相关,种植玉米的TN、TK与MBP负相关,种植桂牧1号杂交象草的pH值与真菌负相关、与放线菌正相关;聚类分析可以将喀斯特峰丛洼地6种土地利用方式划分为4类,其中封育和火烧最好。     

巴西东北部地区土地退化对土壤微生物量和活性的影响

Land degradation causes great changes in the soil biological properties.The process of degradation may decrease soil microbial biomass and consequently decrease soil microbial activity.The study was conducted out during 2009 and 2010 at the four sites of land under native vegetation(NV),moderately degraded land(LDL),highly degraded land(HDL) and land under restoration for four years(RL) to evaluate changes in soil microbial biomass and activity in lands with different degradation levels in comparison with both land under native vegetation and land under restoration in Northeast Brazil.Soil samples were collected at 0-10 cm depth.Soil organic carbon(SOC),soil microbial biomass C(MBC) and N(MBN),soil respiration(SR),and hydrolysis of fluorescein diacetate(FDA) and dehydrogenase(DHA) activities were analyzed.After two years of evaluation,soil MBC,MBN,FDA and DHA had higher values in the NV,followed by the RL.The decreases of soil microbial biomass and enzyme activities in the degraded lands were approximately 8-10 times as large as those found in the NV.However,after land restoration,the MBC and MBN increased approximately 5-fold and 2-fold,respectively,compared with the HDL.The results showed that land degradation produced a strong decrease in soil microbial biomass.However,land restoration may promote short-and long-term increases in soil microbial biomass.     

施氮和灌溉管理下作物产量和土壤生化性质

氮和水对作物生长非常重要,研究施氮和灌溉管理下作物产量、土壤性质以及它们的关系对我国农业科学有重要意义。本文以中国科学院封丘农业生态试验站为平台,研究了施氮(每季施氮150 kg·hm?2、190 kg·hm?2、230 kg·hm?2、270 kg·hm?2,以不施氮为对照)和灌溉(灌溉量达到0~20 cm、0~40 cm、0~60 cm土壤的田间持水量,以雨养为对照)管理下小麦-玉米轮作系统作物产量和土壤生化性质,以及它们之间的关系。研究结果表明,150~270 kg·hm?2施氮量对2008年、2009年玉米产量和2009年、2010年小麦产量无显著影响;灌溉对2010年玉米产量无显著影响,而2008年、2009年玉米产量随灌溉量增大而增加。尽管2008—2011年小麦产量随灌溉量变化趋势不一致,但与雨养相比灌溉提升了小麦产量。施氮在不同程度上提升了土壤全氮和速效氮含量、脱氢酶和脲酶活性、微生物生物量碳、基本呼吸和硝化势,稍微降低了土壤pH并大幅降低了速效磷含量(降幅48.7%~51.6%);灌溉提升了土壤全氮含量和脱氢酶活性,降低了全钾含量、脲酶活性、基本呼吸、硝化势。多元回归分析显示,某些土壤生化性质(全氮、溶解性有机碳、速效磷含量及微生物生物量碳氮、呼吸熵、硝化势)与2009年、2010年玉米产量很好地线性拟合。综上,土壤生化性质因施氮和灌溉发生不同程度的分异,因施氮和灌溉而分异的土壤生化性质能部分地鉴定作物产量。本研究方法可为产量主导因子的筛选及产量估算模型的建立提供一定参考依据。     

土壤微生物量碳氮作为土壤肥力指标的探讨

通过对不同肥力水平黑土、棕壤、黄棕壤、红壤土壤微生物量碳氮的比较,研究和探讨土壤微生物量碳氮与土壤肥力的关系。结果表明:有机肥或无机肥施入土壤均能增加土壤微生物量C、N的含量,且有机肥与无机肥配合有显著的正交互作用。土壤微生物量C、N从北到南的地带性变化规律不强。BC/BN及TC/TN从北到南逐渐减小,BC/TC及BN/TN从北到南逐渐增加。相关分析结果表明:土壤微生物量C、N与全C、全N均呈极显著相关,可以作为指示土壤肥力的重要指标。     

Biological dinitrogen fixation and soil microbial biomass carbon as influenced by free-air carbon dioxide enrichment (FACE) at three levels of nitrogen fertilization in a paddy field

An experiment was conducted in an Andosol paddy field in Shizukuishi (Iwate Prefecture, Japan) to determine the effects of free-air CO₂ enrichment (FACE) on biological N₂-fixation activity and soil microbial biomass C at three levels of N application. Rice (Oryza sativa L. cv. Akitakomachi) plants were grown under ambient CO₂ or FACE (ambient +200 µmol mol^-1 CO₂) conditions throughout the growing season with each treatment having four replicated plots. Three levels of N fertilizer (high, standard and low; 15, 9 and 4 g N m^-2, respectively) were applied to examine the effect of different N availability under both CO₂ conditions. Soil samples were collected at four different times from upper and lower soil layers (0-1-cm and 1-10-cm soil depths, respectively) and analysed for biological N₂-fixation (BNF) activity and microbial biomass C (MBC) by the acetylene reduction and chloroform fumigation-extraction methods, respectively. The amounts of chlorophyll-type compounds (Chls), an index of algal growth, and soil available C were also determined. Compared to the ambient CO₂ treatment, the FACE treatment had significantly higher BNF activity in both the upper and lower soil layers at ripening only in low-N soil and at harvest at all three levels of N fertilization rates. MBC was significantly increased by FACE in both the upper and lower soil layers from the middle to later period of the growing season compared to the ambient CO₂ treatment. The FACE treatment increased the Chls in the upper soil layers at ripening only in low-N soil and at harvest at all three levels of N fertilization rates. The amount of soil available C was not significantly different between FACE and ambient CO₂ treatments in both the upper and lower soil layers throughout the cropping season. From these results it can be concluded that the FACE treatment had a significantly positive influence on BNF activity, MBC and Chls at different levels of N fertilization rates in paddy field during the cropping season.     

Microbial Activity Is Constrained by the Quality of Carbon and Nitrogen under Long-term Saline Water Irrigation

Most important, yet least understood, question, how microbial activity in soil under saline water irrigation responds to carbon (C) varying qualitatively (most labile form to extreme recalcitrant form) with or without maintaining C/N ratio was investigated in an incubation experiment. Soil samples from a long-term saline-water (electrical conductivity, EC ≈ 0, 6, and 12 dS m^?1)- irrigated field were incorporated with three different C substrates, viz., glucose, rice straw (RS), and biochar with or without nitrogen (N as ammonium sulfate, NH₄SO₄) and were incubated at 25 °C for 56 days. Cumulative respiration (CR), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and dehydrogenase activity (DEA) concentrations decreased with increasing EC (P < 0.05), but less so in soils amended with glucose followed by RS and biochar. The addition of N to soils amended with different C substrates significantly decreased CR, MBC, DEA, and available phosphorus (P) concentrations at a given EC level.     

黄土高原半干旱地区撂荒地次生植被演替过程中土壤微生物活性研究

To show the vegetation succession interaction with soil properties, microbial biomass, basal respiration, and enzyme activities in different soil layers (0--60 cm) were determined in six lands, i.e., 2-, 7-, 11-, 20-, 43-year-old abandoned lands and one native grassland, in a semiarid hilly area of the Loess Plateau. The results indicated that the successional time and soil depths affected soil microbiological parameters significantly. In 20-cm soil layer, microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), MBC/MBN, MBC to soil organic carbon ratio (MBC/SOC), and soil basal respiration tended to increase with successional stages but decrease with soil depths. In contrast, metabolic quotient (qCO2) tended to decrease with successional stages but increase with soil depths. In addition, the activities of urease, catalase, neutral phosphatase, β-fructofuranosidase, and carboxymethyl cellulose (CMC) enzyme increased with successional stages and soil depths. They were significantly positively correlated with microbial biomass and SOC (P < 0.05), whereas no obvious trend was observed for the polyphenoloxidase activity. The results indicated that natural vegetation succession could improve soil quality and promote ecosystem restoration, but it needed a long time under local climate conditions.     

喀斯特峰丛洼地不同植被类型土壤微生物量碳氮磷和养分特征

  目的  探讨桂西北喀斯特峰丛洼地不同植被类型的土壤理化性质和微生物碳(MBC)、微生物氮（MBN）、微生物磷（MBP）含量的变化特征及它们之间的关系。  方法  利用生态化学计量方法和Pearson相关性分析方法研究不同植被类型和土层深度对土壤MBC、MBN、MBP含量和土壤养分含量分布特征的影响。  结果  （1）不同植被类型土壤养分含量和MBC、MBN、MBP含量依次为次生林 > 灌木 > 灌草 > 草地 > 耕地;土壤养分垂直分布表现为随着土层深度加深而下降,不同土层间土壤有机碳（SOC）、全氮（TN）、全磷（TP）含量差异显著,土壤MBC、MBN和MBP含量在不同植被类型和不同土层间差异显著,均表现为MBC > MBN > MBP。（2）不同植被类型土壤MBC/SOC和MBP/TP的值较小,MBN/TN的差异较大。不同植被类型的土壤MBC/MBN差异显著,MBC/MBP变化范围较大,MBN/MBP表现为次生林 > 灌草 > 灌木 > 草地 > 耕地。（3）土壤MBC和MBN与SOC、TN、速效氮和速效钾呈显著或极显著正相关,与土壤容重、pH值表现出不同程度的负相关,表明植被恢复过程中土壤MBC和MBN可作为衡量土壤养分的敏感性指标。  结论  不同植被类型的土壤微生物生物量碳氮磷、养分含量和化学计量特征有明显的表聚效应,随着植被的正向演替,土壤结构、养分和微生物群落功能得到显著提高。