The tryptophan requirements of young laying pullets

1. Two experiments were conducted with laying pullets between 32 and 47 weeks of age. In each trial 1 728 White Leghorn and 1 728 crossbred pullets were used.

2. A series of diets of increasing protein content was offered. Protein quality was identical in all diets and tryptophan was demonstrated to be the most limiting amino acid in the protein mixture used.

3. The daily tryptophan requirement of the individual pullet was estimated, by indirect methods, to be 2.25 mg/g egg output plus 10.25 mg/kg body weight. Response curves for flocks of pullets are illustrated. Calculated optimum intakes of tryptophan for various ratios of costs of input to value of output are tabulated.

4. It is estimated that for a flock of mean body weight 1.5 kg, producing 55 g egg mass/hen d and consuming 110 g food/hen d, the optimum dietary tryptophan concentration is 1.7 g/kg when the marginal cost of supplying 1 kg tryptophan is 20 times the marginal value of 1 kg egg output.     

A study of the effect of variation in the calorie‐protein ratio of a medium energy diet and a high energy diet containing sucrose on the efficiency of egg production in caged layers

Literature pertaining to the variation in the protein content of layers’ diets is discussed and the lack of information on the effect of calorie‐protein ratio on food conversion is mentioned. The experiment described in the present paper was designed to test the effect of variations in calorie‐protein ratio on food conversion on a diet of high energy content (1340 k cal/lb.) based on sucrose supplementation and on a medium energy diet (1160 k cal/lb.). At the same time three types of pullet, Thornber 606, Thornber 404 and Light Sussex x Brown Leghorn pullets were compared on the two types of diet, each being fed at three nominal protein levels of 13, 15 and 17 per cent giving a total of eighteen treatments. The pullets were individually recorded for egg production, egg weight and food consumption. Meta‐bolisable energy crude protein determinations were carried out on each of the six diets employed and the actual calorie‐protein ratios calculated.

There was no significant effect on egg production when the calorie‐protein ratio of the diet was raised from 69 (i.e. k cal M.E./lb. per 1 per cent protein) to 90 but an increase to 103 reduced production and egg weight. It was found that maximum efficiency of food conversion was realised at ratios of 76 to 78.

The efficiency of conversion of dietary energy was unaffected by energy concentration in the diet.

The lightweight hybrid (Thornber 606) produced eggs more efficiently than either the Thornber 404 or the crossbred type.     

Phosphorus requirements of poultry. IV. The effects on growing pullets of feeding diets containing no animal protein or supplementary phosphorus

Pullets reared to 18 weeks of age on diets containing no animal protein supplements and to which no source of inorganic phosphorus was added showed no clinical symptoms of rickets and no significant differences in average body weight, feed consumption, efficiency of feed conversion or livability compared with pullets reared on comparable diets supplemented with inorganic phosphorus.

The results indicate that growing pullets are able to use effectively organic sources of dietary phosphorus for growth and bone formation and can be reared satisfactorily on starter diets containing lower levels of total phosphorus than currently recommended.

Replacing protein of animal origin in pullet starter and growers' diets by soyabean meal and DL‐methionine or by extracted soyabean meal and extracted sunflower seed meal did not result in any adverse effects.     

A study of digestibility and biological value of protein in diets fed to colostomised laying pullets to determine their protein requirement

Hybrid pullets were colostomised just after the commencement of laying. A nitrogen‐free diet was used to determine metabolic faecal nitrogen (MFN) and endogenous urinary nitrogen (EUN). The mean figure obtained for MFN/kg. wt./100 g. dry matter/day was 67.2 mg. and for EUN/kg. body weight 3/4/day was 83.3 mg.

High energy diets of about 1350 k cal./lb. containing 13, 15 and 17 per cent protein and medium energy diets of 13 and 17 per cent protein were fed to colostomised pullets to determine their digestibility, biological value and net protein value. In a further experiment a maize‐fishmeal diet of 1370 k cal. ME/lb. and 17 per cent protein, a soyabean‐maize diet of 1330 k cal. ME/lb. and 14 per cent protein and the latter diet supplemented with methionine and/or lysine were also examined. The mean BV and NPV values of the diets considered in Experiment 1 were 60 and 52 respectively.

Addition of methionine to the maize‐soyabean diet increased the BV from 43.9 to 61.6, the addition of lysine to 43.0 and the addition of both to 67.1.

A factorial method of calculating the daily protein requirement of the pullet based on the data for MFN and EUN and the NPV of each diet is presented. The estimate of 13.3 g. protein per day for the hybrid pullet is compared with other estimates in the literature.     

Phosphorus requirements of poultry. V. The effects during the subsequent laying year of feeding growing diets containing no animal protein or supplementary phosphorus

Pullets reared to 18 weeks of age on diets containing no feeding stuffs of animal origin and without phosphorus supplementation showed no significant difference in egg yield compared with similar pullets fed rearing diets containing animal protein and added phosphorus.

During the laying stage, diets in commercial use at this centre gave significantly higher egg yields, better average egg prices due to superior grading results and a higher margin over food cost than the same diets modified to conform with the nutrient recommendations of the National Research Council of America (1960).

Where the supplementary protein in both types of layers' diet was of vegetable origin, egg yield, food cost and margin of income over food cost were all significantly inferior to groups fed comparable diets containing supplementary protein of both vegetable and animal origin.

Soyabean meal and sunflower seed meal gave significantly poorer results when used in combination as the sole protein supplement compared with soyabean meal plus methionine.     

Studies on the effect of lysine and methionine supplementation of a high‐protein maize‐soyabean diet and of a low‐protein barley‐white fish meal diet on the performance of caged layers

Selected literature relating to the amino acid supplementation of layers’ diets is reviewed and the results of two experiments are presented.

In the first experiment a basal soyabean meal diet (14.3 per cent crude protein) was supplemented with L‐lysine and DL‐methionine, singly and together. Supplementation of the basal soyabean diet with the amino acids caused no significant effects on egg production, mean and total egg weight or food conversion efficiency. None of the soyabean diets proved as satisfactory for egg production or food conversion efficiency as a fish meal control diet (16.9 per cent crude protein).

In the second experiment the effect of adding 0.1 per cent each of L‐lysine and DL‐methionine or 0.2 per cent each of l‐lysine and DL‐methionine to a low fish meal diet (2.5 per cent fish meal) was examined. The addition of 0.2 per cent of each amino acid caused an increase in egg production.

Mean egg weight was improved by the addition of amino acids at both levels although this effect was not statistically significant. Comparison of the low fish meal diets with a fishmeal control diet (10 per cent fishmeal) showed that at the 0.2 per cent addition of amino acids, egg production, total egg weight and food conversion efficiency were better than on the 10 per cent fish meal control diet.

Two breeds, light and medium hybrids were used in both experiments.

The results are discussed with reference to their practical significance.     

Effects of age on the tryptophan requirement of laying hens

1. White Leghorn pullets which had been used for an assay of tryptophan requirement between 32 and 40 weeks of age were used for similar determinations between 63 and 73 and, after a moult, from 97 to 106 weeks of age.

2. A tryptophan‐limiting protein mixture was used and by dilution seven dietary protein contents were produced, supplying from 0.84 to 1–92 g tryptophan/kg diet. The diet of lowest protein content was also sup‐lemented with free tryptophan. These diets were fed in experiments using 24 groups of 72 pullets at 63 to 73 weeks and 45 groups of 21 hens at 97 to 106 weeks.

3. The relationship between egg output and tryptophan intake was the same in moulted hens as in young pullets, but pullets of 63 to 73 weeks of age yielded a different response curve; more tryptophan being needed for a given egg output.

4. It is concluded that tryptophan required, per day, does not decrease during the first laying year, despite a decrease in rate of egg output.     

Incorporation of triticale in layer diets

1. Diets containing either maize or triticale were fed unsupplemented or supplemented with meat meal or groundnut oil to White Leghorn pullets for two periods of 11 weeks each.

2. The egg production of birds fed on the unsupplemented triticale diet was significantly higher than that of the birds fed on the unsupplemented maize diet, but was not significantly different from that of the birds fed on the maize plus meat meal diet.

3. Protein quality of the unsupplemented triticale diet as judged by efficiency of nitrogen retention was similar or inferior to that of the unsupplemented maize diet.

4. The higher protein content of triticale and the increased intake of these diets seemed to be responsible for the better performance observed.

5. Addition of groundnut oil to the triticale diet did not improve either egg weight or production.

6. It is concluded that the quantitative substitution for triticale of maize does not adversely affect egg production and egg weight.     

Replacement of groundnut cake by dried poultry manure in the diets of laying hens

1. The effect on food consumption by pullets when dried poultry manure (DPM) replaced part of dietary groundnut cake depended on the strain of the birds: in two of the three hybrids food consumption was not affected when up to 100 g DPM/kg was fed.

2. Increases in food consumption were accompanied by increases in egg production.

3. The food required per dozen eggs was improved by up to 100 g dietary DPM/kg and variation in the sizes of eggs laid depended on the hybrid.

4. Concentrations of DPM up to 100 g/kg did not affect the water‐soluble nitrogen content of the egg but the crude albumen nitrogen content was depressed at concentrations of 80 g/kg or above.

5. Feeding DPM to laying pullets depressed body weight.     

The effects of crude and refined low erucic acid rapeseed oils in diets for laying hens

1. The effects of feeding diets containing 15% crude or refined low erucic acid Span rapeseed oils, refined regular rapeseed oil or lard to pullets on productive performance and the lipids of egg yolk were determined.

2. Productive performance was best with the low‐fat control diet, less good with the crude low erucic acid Span oil or lard‐containing diets, intermediate with the diet containing refined Span oil and worst with the diet containing regular rapeseed oil.

3. The various dietary treatments did not affect total lipid content of egg yolk but did affect the fatty acid composition of egg yolk lipids.

4. No substantial amounts of erucic acid were found in the eggs indicating that the hen is able to control the lipid concentration in egg yolk though control of the fatty acid composition of lipids is poorer.     

A formal method of determining the dietary amino acid requirements of laying‐type pullets during their growing period

1. The amino acid requirements of laying type pullets during the growing period can be estimated by measuring the growth of different components of the body and making use of nutritional constants that define the amount of each amino acid that is required for the production of the tissues being formed.

2. In this experiment, carcase analyses of each of three breeds of pullets were conducted at weekly intervals throughout the growth of the pullets, to 18 weeks of age. Measurements were made of body weight, gut‐fill and feather weight, and chemical analyses consisted of water, protein, lipid and ash measurements of both the body and the feathers. Each age group comprised 10 birds of each breed.

3. Gompertz functions accurately estimated the growth of both body protein and feather protein, to 18 weeks of age, from which the rate of growth of these two components of the body could be estimated. The mature weight of pullets was overestimated by the Gompertz growth curve, which may indicate that a pullet ceases to increase in body protein content once sexual maturity has been reached.

4. Using allometric relationships between the chemical components of the body and of feathers, all the components of growth could be estimated from the growth of body protein and feather protein. These components were then added together to determine the growth rate of the body as a whole.

5. The daily amino acid requirements for 4 functions were calculated, namely, those for the maintenance of body protein and feather protein, and for the gain in body protein and feather protein. These requirements were then summed to determine the requirement of pullets on each day of the growing period.

6. Using the ‘effective energy’ system, the amount of energy required by these pullets was calculated for each day of the growing period, from which the desired daily food intake of the pullets could be predicted. By dividing the amino acid requirement by this daily food intake it was possible to determine the concentration of amino acids that would be needed in the diet in order to meet the requirements of a pullet.

7. The results indicate that the ratio between the requirement for lysine and for methionine and cysteine changes dramatically during the growing period, negating the concept of a fixed ratio between all the amino acids during growth.

8. The above process is the first step in determining the optimal feeding programme for a population of pullets of a given genotype. The constraining effects, of the diet being offered and of the environment in which the pullets are housed, on the food intake and growth rate of each pullet have to be estimated, and such a theory can then be expanded to include all the individuals in the population. Only by the use: of simulation models can all these constraining effects be considered simultaneously.     

The use of rapeseed meals and a mustard seed meal as a protein source in diets for laying pullets

1. Three experiments with laying pullets were carried out in which rapeseed meals (B. napus and B. campestris) and a mustard seed meal (B. juncea) formed 12% of the diet.

2. Dietary rapeseed had no effect on the onset of lay when fed from 17 weeks of age but caused a delay when fed from 10 weeks of age: changing to a control soyabean diet for the laying period resulted in normal egg production.

3. Diets containing B. napus but not B. campestris consistently depressed egg production and reduced egg weight in one experiment. Mustard seed meal did not affect performance.

4. Significant number of eggs with a “fishy” taint were laid by brown‐egged birds fed on a diet containing rapeseed.

5. Thyroids of birds fed on rapeseed meal diets during the laying period were significantly heavier than those of birds fed on diets containing mustard seed or soyabean.

6. The enlargement of the thyroid gland is probably related to the oxazolidinethione content of the rapeseed.     

Calcium and phosphorus levels in the diet of laying birds

Two hatches of crossbred pullet chicks were brooded and reared to 18 weeks of age on diets which conformed to the recommendations of the National Research Council (N.R.C.) of America for calcium and phosphorus. The pullets of each hatch were then divided at random into six laying groups. Three groups were fed diets containing 2.25, 3.14 and 5.0 per cent respectively of calcium without supplementary phosphorus and the other three groups were fed diets of the same graded calcium levels but with supplementary phosphorus to give a total inorganic phosphorus content of 0.45 per cent. When the rate of egg production in the groups receiving 2.25 per cent calcium exceeded 70 per cent an ad libitum supply of limestone grit was provided and the intake recorded. Over the twelve months of lay this resulted in an average total dietary intake level of nearly 2.65 per cent calcium.

Egg yield was not significantly influenced by the diets, except that the production of birds fed on the diet containing 5 per cent calcium without supplementary phosphorus was significantly depressed. Food intake was not adversely affected by the higher levels of ground limestone; in fact, there was a positive regression of food consumption on the percentage of calcium in the diet. Because of the greater food intake and the lower egg production, food consumed per dozen eggs and cost of food per dozen eggs were higher in the groups receiving 5 per cent dietary calcium without phosphorus supplementation, compared with groups which received lower dietary levels of calcium. There were, however, no significant differences between the various groups in income or in the margin of income over costs, per bird or per dozen eggs.

The data on egg grading showed that over the two experiments and at all levels of calcium there was no evidence of any influence of phosphorus on the combined percentage of large and standard eggs. The average percentage for the two diets with the 5 per cent level of calcium was significantly greater than the combined average for all the other diets.

The average percentage of cracked eggs for the groups with diets containing 5 per cent calcium was 9.4 per cent compared with an average of 11.1 per cent for all the other diets, but the difference was not significant.

The results suggest that there is no economic advantage in raising the calcium content of the basal diet used in these experiments beyond the level of 3.14 per cent and that at this level there appears to be no need with this basal diet to supplement the phosphorus content.     

Comparison of nutritive values of groundnut oil cake and Niger oil cake for laying pullets

1. The nutritive value of Niger oil cake (Guizotia abyssinica, Cass.) as a protein supplement for layers’ diets has been assessed.

2. Replacing groundnut oil cake (GNC) by Niger oil cake (NC) on an isonitrogenous basis, did not affect egg production, egg weight or the amount of food required per dozen eggs.

3. The percentage retention of nitrogen from diets containing 30% GNC or 30% NC was similar.

4. The ME value of NC used was 3025 kcal/kg.

5. It is concluded that NC can replace GNC in layers’ diets.     

Asociation of plasma 5'‐nucleotidase and alkaline phosphatase with production traits in chickens: Effects of age and housing systems

1. The housing system, age of pullets and their interaction had a significant effect on plasma 5'‐nucleotidase and alkaline phosphatase activities.

2. Activities were higher in birds in cages than in those on the floor, reflecting perhaps the stress of caging. Activities increased with age.

3. Activities were higher in pullets selected for higher production.

4. The type of housing had no effect on egg production, but age at first egg, egg weight and 40‐week body weight were found to be affected significantly.     

Whole grain diets for pullets

1. Three experiments, each involving over 400 pullets, were conducted into the use of whole grain rearing diets. They were concerned with the cereal to be used, the age from which such diets may be used, and the value of a vitamin‐trace element supplement. Each experiment was performed on a brown‐egg and a white‐egg strain.

2. Whole maize was found to be unsuitable.

3. Whole wheat or whole barley diets should not be introduced before the age of 10 weeks to avoid unacceptably high mortality due to cannibalism. Thereafter the principal results are reduced body weight at caging and retarded sexual maturity.

4. The addition of a vitamin‐trace element supplement had some effect in reducing mortality when whole grain diets were introduced before 10 weeks of age.     

The effects of early and late maturity on the protein requirements of pullets

Growing pullets were exposed to two light patterns which caused a difference in sexual maturity of 5 weeks. From 28 to 38 weeks of age they were fed diets containing crude protein levels of 7.0, 8.5, 10.0, 11.5, 13.0 and 14.5 per cent. Yellow maize provided 45 per cent and soyabean meal 55 per cent of the protein in all six diets.

Rate of lay, egg weight and body weight were greater in the late maturing flock than in the early flock at the start of the assay and throughout the assay period. The late maturing pullets required more protein to reach and maintain their maximum potential than the early birds, presumably because their potential output was greater. The late birds showed a diminishing but continued response to protein up to the highest level fed (14.5 per cent corresponding to 23 g. protein per bird day). The early maturing pullets showed no response to dietary protein levels beyond 11.5 per cent and their estimated protein requirement was about 16 g. per bird day.

When limiting amounts of protein were fed (7.0–10.0 per cent of the diet) the two flocks achieved similar levels of egg output. In both flocks and throughout the assay, body weight and egg weight fell at the two lowest levels and increased at the three highest levels of protein.

It is concluded that the protein requirements of early maturing and late maturing flocks of pullets may differ, but only in a way which corresponds directly with their different potential outputs. In the late flock, which had the higher protein requirement, excellent production was obtained with a diet containing 14½ per cent crude protein all of which came from vegetable sources.     

Comparisons of frequencies and egg shell characteristics of broken and intact eggs within diverse populations of chickens

1. Differences were found among eight populations of White Plymouth Rock pullets in the frequency of broken eggs.

2. The shells of such eggs were thinner than those of intact eggs in seven of the populations.

3. The number of defective eggs, the specific gravity of the eggs, and the percentage hen‐day egg production of normal eggs were significantly correlated within populations with the incidence of broken eggs.

4. No significant correlations were found within populations between the incidence of egg breakage and either egg weight, body weight, or shank length.     

Heritabilities and genetic correlations in related dwarf and normal broiler populations 1

1. Heritability estimates were higher for 8‐week body weight in dwarf than in normal broiler populations due to the maternal effects of dwarf dams.

2. The dwarfing gene dw did not induce new genetic variability for egg weight, 30‐week body weight and age at sexual maturation.

3. Genetic correlation estimates showed that the selection for 8‐week body weight will increase egg weight in dwarf pullets more than in normals.

4. Within a dwarf population it should be possible to increase 8‐week body weight without influencing the mature size of pullets.     

Effect of feeding low lysine starters and developers on growth,sexual maturity and subsequent performance of broiler breeder pullets 1

Pullets that were fed diets containing 0.53 per cent and 0.42 per cent lysine grew at a slower rate and matured later than those that were given adequate amount of lysine. The latter pullets converted their food more efficiently than did the restricted pullets with the exception of the group fed the ration containing 0.53 per cent lysine for the first 12 weeks. The restricted pullets consumed less food during the growing period. A smaller bird was produced on less food. The restricted pullets were also lighter than the control pullets at the end of the laying season. A saving in food costs is indicated because the smaller hen would require less food for maintenance.

There was a higher mortality in the pullets fed a ration containing 0.53 per cent lysine during the growing period. The mortality in the group fed the ration containing 0.42 per cent lysine from 7 to 22 weeks of age was the same as that of the control. There were no differences in mortality during the laying season that could be related to methods of rearing the pullets.

During the laying season, the highest rate of lay was obtained from hens which had been fed the ration containing 0.42 per cent lysine from 7 to 22 weeks of age. Hens fed on the ration containing 0.53 per cent lysine from o to 4, 8 or 12 weeks of age showed no improvement in rate of lay as compared with controls.

Hens fed the ration containing 0.42 per cent lysine from 7 to 22 weeks laid fewer small eggs at the beginning of the laying season than did hens receiving adequate amounts of lysine throughout or fed the ration containing 0.53 per cent lysine from o to 4, 8 or 12 weeks. The hens fed the latter ration laid smaller eggs throughout the laying season.

No differences were found between the fertility, hatchability or embryonic mortality of eggs obtained from hens fed on lysine deficient diets during growth and those fed on diets adequate in lysine.