共查询到20条相似文献，搜索用时 15 毫秒
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This case report describes the laparoscopic approach for removal of cystic intra-abdominal testicles in the standing colt. One 3-year-old Tobiano and one 2-year-old Warmblood colts were referred for abdominal cryptorchidectomy. The horses were clinically and ultrasonographically examined and a presumptive diagnosis of unilateral abdominal cryptorchidism was made. A laparoscopic approach via the flank was used to localise each abdominal testicle. In both colts the abdominal testicle was enlarged and cystic. Each spermatic cord was ligated and fluid was aspirated from the testicle. By reducing the size of the mass minimally invasive removal through an enlarged instrumental portal was possible. Histopathology revealed a cystic rete testis in the Tobiano and a teratoma in the Warmblood. In these cases the cystic enlarged testicles were nonpainful and were incidental findings. A cystic testicle might be developmental (Tobiano case) or arise due to neoplastic transformation (Warmblood case). The laparoscopic approach for enlarged cyst-like testicles in the standing horse offers a secure minimally invasive method for removal. 相似文献
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John M. Losonsky DVM MS Louise C. Abbott PhD DVM Igor V. Kuriashkin MD PhD 《Veterinary radiology & ultrasound》1997,38(4):251-258
Computed tomography (CT) images of the feline nasal cavity and paranasal were acquiredfrom noraml adult cats. Good resolutin and amatomic detail were obtained from the CT images using soft tissue formatting. A desciption of normal feline nasal cavity and paranasal sinus anatomy using CT is presented. 相似文献
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猫传性腹膜炎(FIP)是由猫冠状病毒引起的猫科常见致死性传染病。为分析成都地区猫传性腹膜炎病毒(FIPV)部分基因的序列特征,本研究从11只FIP患猫的腹水中提取总RNA,采用普通PCR或套式PCR对FIPV的非结构蛋白基因ORF3 (3a、3b和3c)、7b以及S基因七肽重复区1 (HR1)序列进行PCR扩增并测序。结果显示:检测到FIRV的上述几个基因均呈遗传多样性,序列存在很大的个体间差异。3a和3b基因主要表现为氨基酸突变,3c基因在约89%的样品中存在截短。7b基因有7个位点的氨基酸被完全替换。S基因HR1序列融合肽的1045位甲硫氨酸被亮氨酸替换,其下游的1057位丝氨酸被丙氨酸替换。遗传进化树显示,本实验扩增的FIRV 7b及S基因HR1序列与GenBank中国外FIPV相应基因序列亲缘性较高。推测3c基因的截短和/或某些氨基酸的替换的共同作用与病毒的致病性有关。本研究结果为FIP的临床确诊提供了分子诊断依据,增加了临床诊断结果的可靠性。 相似文献
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Meghan Woodland LeeAnn Pack Paul Rist Bronwyn Crane 《Veterinary radiology & ultrasound》2014,55(4):368-373
It is not always possible to identify female cats that have undergone previous ovariohysterectomy based on physical examination alone. An easy, cost‐effective method for screening female cats for reproductive status would be helpful for avoiding unnecessary exploratory laparotomies. The purpose of this prospective study was to compare diagnostic sensitivities of digital radiography, ultrasonography, and positive contrast vaginourethrography for determining reproductive status in female cats. Sixty‐seven recently euthanized female cats of unknown medical history and reproductive status were randomly selected and included in the study. Digital abdominal radiography, digital abdominal radiography with compression, abdominal ultrasonography, and positive contrast vaginourethrography were performed in sequence by a board‐certified veterinary radiologist and a second‐year radiology resident. Immediately following diagnostic imaging procedures, necropsy was performed. Ultrasonography of the uterus had the highest sensitivity (86%) for determining reproductive status of all the imaging modalities tested. The specificity was 88%, and the positive predictive value and negative predictive value were 96% and 68%, respectively. The calculated sensitivities and specificities of other modalities were as follows: digital radiographs (28%, 100%), digital compression radiographs (58%, 100%), and vaginourethrography (32%, 100%). Based on McNemar's test statistic, there was a significant difference in the sensitivity of ultrasound compared to digital radiographs (P ≤ 0.05), compression radiographs (P ≤ 0.05), and vaginourethrogram (P ≤ 0.05). Findings from the current study indicated that ultrasonography is a sensitive diagnostic test for determining reproductive status in female cats. Although more readily available in private practice and shelters, digital radiography and vaginourethrography are not reliable predictors of reproductive status. 相似文献
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Thirty-six formalin-fixed, paraffin-embedded enucleated globes from cats with a diagnosis of diffuse anterior uveal melanoma were obtained. Sections of tumor were excised, deparaffinized, and subjected to nested polymerase chain reaction (PCR) to identify proviral DNA sequences from the feline leukemia virus (FeLV)–feline sarcoma virus (FeSV; 36 eyes), and the feline immunodeficiency virus (FIV; 18 eyes). All samples tested were negative for FIV DNA. Three samples were positive for FeLV–FeSV DNA. This is the first reported evidence of a possible link between naturally occurring feline anterior uveal melanoma and the presence of FeLV–FeSV DNA. 相似文献
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Christopher P. Ober DVM Kathy Spaulding DVM Edward B. Breitschwerdt DVM David E. Malarkey DVM PhD Barbara C. Hegarty BA 《Veterinary radiology & ultrasound》2004,45(5):458-465
Two dogs with testicular swelling were sonographically diagnosed with orchitis and were subsequently diagnosed with Rocky Mountain spotted fever (RMSF). Use of both gray scale and color Doppler sonography allowed for differentiation of orchitis from neoplasia and torsion. While only experimentally induced RMSF is reported to cause orchitis in dogs, it should be considered in any dog with vascular insult to the testes, especially when other signs of systemic illness are involved. 相似文献
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2月龄雄性小尾寒羊12只,随机分为3组,Ⅰ组饲喂基础日粮(Mo 5.61 mg/kg),Ⅱ、Ⅲ组饲喂高钼日粮(在基础日粮中分别添加30、60 mg/kg的钼构成).120 d后取睾丸,检测精液品质,以流式细胞术和透射电镜的方法观察睾丸的变化.结果表明,与Ⅰ组相比,Ⅱ、Ⅲ组睾丸指数显著降低(P＜0.01),精子密度和精子活力显著下降(P＜0.01),Ⅱ、Ⅲ组之间差异显著(P＜0.05);Ⅱ、Ⅲ组睾丸细胞G0/G1期显著升高,S期,G2 +M期和增值指数(PI)显著降低(P＜0.05或P＜0.01),Ⅱ、Ⅲ组之间各项指标差异极显著(P＜0.01);Ⅱ、Ⅲ组睾丸细胞线粒体肿胀、空泡化,内质网扩张,胞核染色质边集,精子顶体膜模糊或缺损,Ⅲ组出现核溶解的现象.结论:日粮钼含量≥30 mg/kg可造成小尾寒羊睾丸超微结构损伤,导致精液品质和生精功能下降. 相似文献
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Trimmer AM Griffin CE Rosenkrantz WS 《Clinical Techniques in Small Animal Practice》2006,21(3):157-161
Feline allergen specific immunotherapy (ASIT) is considered to be a safe and effective treatment for feline atopy. ASIT is defined as the practice of administering gradually increasing quantities of an allergen extract to an allergic subject. The purpose of which is to reduce or eliminate the symptoms associated with subsequent exposures to the causative allergen. ASIT offers an effective and safe treatment option for cats. Reported success rates range for 60 to 78% in feline atopic patients. Additionally, the reported incidence of side effects in feline atopic patients undergoing ASIT is very low and mainly anecdotal. 相似文献
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Ocular sarcoma was diagnosed by light microscopic examination in enucleated globes ( n = 4), orbital tissue biopsy ( n = 1) and ocular evisceration contents ( n = 1) from six cats. To determine if feline leukemia virus (FeLV) or a replication-defective FeLV, feline sarcoma virus (FeSV), was present in these ocular sarcomas, immunohistochemistry (IHC) and polymerase chain reaction (PCR) for FeLV were utilized. Immunohistochemical staining for FeLV glycoprotein 70 (gp70) was performed on all six formalin-fixed, paraffin-embedded tumors using an avidin–biotin complex technique. DNA was extracted from each specimen and a 166 bp region of the FeLV long-terminal repeat (LTR) was amplified by PCR. All tumors were composed primarily of spindle cells; two neoplasms had PAS-positive basement membrane enveloping areas of spindle cells. All tumors involved the uvea and five of six tumors showed transcleral extension, one of which invaded the optic nerve. Immunohistochemical staining for FeLV gp 70 was negative. PCR to amplify a portion of the FeLV LTR was negative. Based on these findings of these limited number of cases, FeLV/FeSV may not play a role in the tumorigenesis of feline ocular sarcomas. However, additional tumors representing all morphological subtypes should be investigated for the presence of viral antigen and DNA. It is important to determine the etiology and pathogenesis of these malignant ocular sarcomas. If the cell of origin and pathogenesis involve ocular and lenticular injury, and FeLV/FeSV is not present, then the clinical management of cases of feline ocular trauma, uveitis and glaucoma may prevent the development of this tumor. 相似文献
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Pinches MD Diesel G Helps CR Tasker S Egan K Gruffydd-Jones TJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(2):141-147
BACKGROUND: Screening tests for feline retroviruses are thought to have high sensitivity and specificity, although previous studies that evaluated these tests have limitations. Novel statistical approaches have been developed that allow the estimation of sensitivity and specificity in situations where the true state of the disease in individual animals cannot be assured. OBJECTIVE: The purpose of this study was to evaluate the sensitivity and specificity of a variety of retrovirus tests, including some screening tests, in a population of cats potentially infected with either feline leukemia virus (FeLV) and/or feline immunodeficiency virus (FIV) by using a Bayesian statistical approach. METHODS: Four hundred and ninety blood samples from cats being evaluated for FIV infection were tested by 2 rapid immunomigration tests (Witness single [WS], Witness combi [WC]) and a plate-based ELISA (Petcheck) for FIV antibody, and by a newly designed real-time polymerase chain reaction (PCR) assay for FIV provirus. Four hundred and ninety-five blood samples from cats being evaluated for FeLV infection were tested by 2 rapid immunomigration tests (WS, WC) and a plate-based ELISA (Petcheck) for FeLV antigen, and by a FeLV virus isolation technique. Results were then analyzed by using a Bayesian statistical method. RESULTS: For FIV tests, median sensitivity estimates were 0.98 for WS, 0.97 for WC, 0.98 for ELISA, and 0.92 for PCR. Median specificity estimates were 0.96 for WS, 0.96 for WC, 0.93 for ELISA, and 0.99 for PCR. For FeLV tests, median sensitivity estimates were 0.97 for WS, 0.97 for WC, 0.98 for ELISA, and 0.91 for virus isolation. Median specificity estimates were 0.96 for WS, 0.96 for WC, 0.98 for ELISA, and 0.99 for virus isolation. CONCLUSIONS: The use of Bayesian statistical methods overcomes a variety of methodologic problems associated with diagnostic test evaluations, including the lack of a definitive reference test. The sensitivity and the specificity of all 6 evaluated screening tests was high: however, specificity estimates were slightly lower than those reported by most recent studies. 相似文献
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Louise Katherine Bartolac Jenna Louise Lowe George Koustas Christopher Gerald Grupen Cecilia Sjöblom 《Animal Science Journal》2018,89(9):1230-1239
The aim of this study was to determine the most efficient vitrification protocol for the cryopreservation of day 7 in vitro produced (IVP) porcine blastocysts. The post‐warm survival rate of blastocysts vitrified in control (17% dimethyl sulfoxide + 17% ethylene glycol [EG] + 0.4 mol/L sucrose) and commercial media did not differ, nor did the post‐warm survival rate of blastocysts vitrified in medium containing 1,2‐propandiol in place of EG. However, vitrifying embryos in EG alone decreased the cryosurvival rate (55.6% and 33.6%, respectively, p < .05). Furthermore, the post‐warm survival rates of blastocysts vitrified with either trehalose or sucrose as the non‐penetrating cryoprotectant did not differ. There was also no significant difference in post‐warm survival of blastocysts vitrified in control (38°C) media and room temperature (22°C) media with extended equilibration times, although when blastocysts were vitrified using control media at room temperature, the post‐warm survival rate increased (56.8%, 57.3%, 72.5%, respectively, p < .05). The findings show that most cryoprotectant combinations examined proved equally effective at supporting the post‐warm survival of IVP porcine blastocysts. The improved post‐warm survival rate of blastocysts vitrified using media held at room temperature suggests that the cryoprotectant toxicity exerted in 22°C media was reduced. 相似文献
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Rie KOIDE Shoichi SAKAGUCHI Takayuki MIYAZAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):565-569
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered
in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic
nephritis, little is known about its biological characteristics. In this study, we
established a quantitative assay of FmoPV by using an indirect immunofluorescence
technique. Viral titers of FmoPV were determined in one week. Treatment with
polybrene® or trypsin which was previously used in virus isolation did not
augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at
least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2
min, respectively. The biological characteristics of FmoPV reported here will be
beneficial for establishing an efficient virus isolation method and will provide important
information to take a measure to reduce the risk of FmoPV infection. 相似文献
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B.A. Selcer DVM S.M. Newell DVM A.E. Mansour MS J.W. Mccall PhD 《Veterinary radiology & ultrasound》1996,37(1):37-44
Eighteen cats were exposed to Dirofikria immitis infected mosquitoes. Thoracic radiography was performed prior to exposure and at 5, 7, and 9 month intervals following exposure. Immunologic testing for adult heartworm antigen was performed on days 168,195,210,224,237,254 and 271 post infection. Necropsies were performed on all cats. Adult heartworms were found in 61% of the exposed cats. Radiographic findings in heartworm positive cats included bronchointerstitial lung disease, lobar pulmonary arterial enlargement and pulmonary hyperinflation. In most heartworm positive cats, lobar arterial enlargement resolved as the disease progressed while pulmonary hyperinflation progressively became more common. Pulmonary patterns in heartworm positive cats remained abnormal throughoutthe study while abnormal pulmonary patterns resolved in over 50% of the heartworm negative cats. Cardiomegaly was seen in less than 50% of the cats with adult heartworms at necropsy. This study suggests that the radiographic appearance of heartworm disease is variable and radiographic changes are dependent on the time post infection at which cats are evaluated. Echocardiographic examinations were randomly performed on 16 of 18 cats. Heartworms were identified in 7 cats. No false positive identifications were made. Persistent pulmonary disease accompanied by resolving vascular disease in heartworm cats with pulmonary hyperinflation may be difficult to distinguish from cats with feline allergic lung disease. Echocardiograms may be helpful in identifying adult heartworms in cats in which the radiographic signs or immunodiagnostic data are insufficient to provide a diagnosis. 相似文献
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公牛精液品质不仅与种公牛的饲养水平有关,而且与公牛本身的遗传基础有关。公牛产弱精或不产精严重影响了养牛业的经济效益。为了解精液品质的遗传机制,发展优质、高效的制种业,作者从精子发生过程入手,着重介绍在精子发生过程中特异的细胞分化活动中发挥至关重要作用的基因或基因家族,包括tsMCAK、Minna53及TLF等,讨论了与精子发生有关的转录因子基因、细胞周期相关基因、原癌基因、细胞凋亡相关基因的研究成果,为从事该领域研究的工作者提供可靠的参考信息,并探讨了今后如何对精液品质遗传基础进行研究。 相似文献
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Cathy Beck BVSc Jennifer A. Charles BVSc Alistair A. Maclean BVSc 《Veterinary radiology & ultrasound》2001,42(4):355-357
A 16-year-old thoroughbred stallion developed sudden swelling of the left testicle. The stallion had previously been regarded as a unilateral cryptorchid. Ultrasound examination of the left testicle revealed a diffusely heterogeneous parenchyma. The testicle was diffusely hypoechoic with ill defined regions of hyperechogenicity giving the appearance of hypoechoic nodules throughout the testicular parenchyma. No normal testicular tissue was identifiable. An echogenic band, representing a pseudocapsule could be seen surrounding the testicle. Histopathologic diagnosis a seminoma. 相似文献
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免疫去势对公鸡生长发育及睾丸组织结构和功能的影响 总被引：3，自引：0，他引：3
用成鹅睾丸组织匀浆加佛氏完全佐剂对１０日龄、３０日龄公鸡人工免疫１次或２次，与对照组相比，不同日龄实验组鸡的体重均增大，以６０日龄最明显（Ｐ＜０．０５）；鸡冠长、高及肉垂较小，外貌变化不大，均有鸣叫和交尾行为；采精量少（Ｐ＜０．０１），甚至采不出来；人工授精和孵化无胚胎成活，未受精；左右睾丸的重量、体积和精细管管径均较小（Ｐ＜０．０５或Ｐ＜０．０１），在１５０日龄、２７０日龄，仅有个别公鸡的精细管管内有极少量的精子。结果表明，用免疫去势法可达去势的目的。 相似文献