Molecular assessment of genetic diversity in winter barley and its use in breeding

Summary During the last decades extensive progress has been achieved in winter barley breeding with respect to both, yield and resistance to fungal and viral diseases. This progress is mainly due to the efficient use of the genetic diversity present within high yielding adapted cultivars and – with respect to resistance – to the extensive evaluation of genetic resources followed by genetic analyses and introgression of respective genes by sexual recombination. Detailed knowledge on genetic diversity present on the molecular level regarding specific traits as well as on the whole genome level may enhance barley breeding today by facilitating efficient selection of parental lines and marker assisted selection procedures. In the present paper the state of the art with respect to virus diseases, i.e. Barley mild mosaic virus, Barley yellow mosaic virus, and Barley yellow dwarf virus is briefly reviewed and first results on a project aiming on a genome wide estimation of genetic diversity which in combination with data on yield and additional agronomic traits may facilitate the detection of marker trait associations and a more efficient selection of parental genotypes are presented. By field tests of 49 two-rowed and 64 six-rowed winter barley cultivars the genetic gain in yield for the period 1970–2003 was estimated at 54.6 kg ha⁻¹ year⁻¹ (r² = 0.567) for the six-rowed cultivars and at 37.5 kg ha⁻¹ year⁻¹ (r² = 0.621) for the two-rowed cultivars. Analysis of 30 SSRs revealed a non-homogenous allele distribution between two and six-rowed cultivars and changes of allele frequencies in relation to the time of release. By PCoA a separation between two and six-rowed cultivars was observed but no clear cut differentiation in relation to the time of release. In the two-rowed cultivars an increase in genetic diversity (DI) from older to newly released cultivars was detected.     

Genetic improvement of spring barley cultivars grown in eastern Canada from 1910 to 1988

Summary Spring barley is an important feed crop in eastern Canada, and the development of high-yielding, high grain protein cultivars is desirable. This study was conducted to assess the impact of breeding on the yield and protein aspects of cultivar development, and to identify related changes in plant characteristics which may have been altered over time. A 3-year field experiment was conducted to evaluate twenty six-rowed spring barley genotypes representing the majority of cultivars developed from 1910 to 1988 for eastern Canada. The yields of barley cultivars released from 1935 to 1988 increased at a rate of about 0.03 t ha^-1 yr^-1, and showed no evidence of having reached a plateau. Increases in yield were associated with higher total dry matter production and harvest index, reduced plant height and increased lodging resistance. No consistent change in main stem or tiller yield components was observed. Grain protein concentration decreased progressively with time, especially with the newer cultivars. Reduction in grain protein concentration was not associated with lower protein content on a per grain basis, but rather with an increase in the amount of non-structural carbohydrate per grain. Total plant and grain N accumulation showed positive trends with time. No trends were observed for N harvest index, apparent post-heading N uptake, N retranslocation, and retranslocation efficiency. Thus, while the newer cultivars accumulated more total and grain N, proportional N partitioning to the grain was not altered.     

Agronomic performance of winter wheat grown under highly divergent soil moisture conditions in rainfed and water‐managed environments

Even in the temperate climates of Europe, increasing early season drought and rising air temperature are presenting new challenges to farmers and wheat breeders. Sixteen winter wheat (Triticum aestivum L.) genotypes consisting of three hybrids, six line cultivars and two breeding lines from Germany as well as five line cultivars from France, Austria, Slovakia, Hungary and the Ukraine (referred to as “exotic” lines) have been included in this study. The genetic materials were evaluated over three growing seasons under a range of soil moisture regimes at the three North German sites Braunschweig (irrigated and drought‐stressed), Warmse (rainfed) and Söllingen (rainfed). The average grain yields in the twelve growth environments (water regime × season combinations) ranged from 6.1 to 13.5 t ha^?1. The exotic lines showed little evidence of specific phenological adaptation to drought although they are frequently faced with water scarcity in their countries of origin. The hybrids and German lines exhibited higher regression coefficients (b_i) to environmental means than the exotic lines, indicating particular adaptation to favourable growing conditions. The phenotypical correlations of grain yield between the various environments were high, ranging for instance from 0.6 to 0.8 for the irrigated and drought‐stressed environments at Braunschweig. It is thus expected that in the foreseeable future continued selection aiming at high yield potential will suffice as a means to counter the expected increase in droughts.     

Breeding Progress in Grain Yield and Selected Agronomic Characters of Winter Barley (Hordeum vulgare L.) over the Last Quarter of a Century

This study gives estimates of breeding progress achieved in winter barley (Hordeum vulgare L.) since the 1960′s in grain yield and the change in several morphological traits for seventeen historically important cultivars: 12 six-rowed and 5 two-rowed, grouped into four epochs according to the period of major commercialization in Italy. All genotypes were evaluated across a range of soil fertility and climatic environments. The modern six-rowed cultivars have shown a consistent increase in tillers/m² and seeds/spike (16 and 28 %, respectively) over the local populations. In the modern two-rowed cultivar ‘Igri’ the increase in tillers/m² and seed weight were, respectively, 20 and 14 % snd seeds/spike decreased by 20 % over the local populations. The modern six-rowed and two-rowed cultivars have less lodging, are earlier and shorter than the old varieties and local populations. In the last quarter of a century, the breeding progress for gram yield has been increased by 52 and 74 kg/ ha/year or 0.75 and 1.1 % per year respectively, for six-rowed and two-rowed genotypes: while the gain in biomass yield in the same period was not uniform for six-rowed cuitivars and increased by 64 kg/ha/ year or 0.46 % in two-rowed cultivars. The grain yield improvement in the modern six-rowed and two-rowed cultivars is the result of a better partitioning of the photosynthetic products into the grain.     

Genetic improvement of yield responsiveness to nitrogen fertilization and its physiological determinants in barley

Traditionally, barley in Argentina has been cultivated in low-yielding environments. A study was conducted to test whether breeding for improved performance under these conditions would have also improved the responsiveness to nitrogen availability. Four cultivars of two-rowed malting barley (released in 1944, 1960,1982 and 1998) were grown under 4 rates of nitrogen fertilizer at sowing (20, 50, 110and 160 kgN ha^-1). All cultivars increased their yield with the increase in soil nitrogen. But yield of modern cultivars responded more strongly than yield of old ones. For modern cultivars, increase in grain yield was of 12 ± 0.6 kgha^-1 for each 10 kg ha^-1 of increase in the mean yield (environmental index). Absolute values of genetic gain were related to nitrogen availability: 1.59, 2.58, 4.52 and 4.29 g m^-2 year^-1 for the N₂₀, N₅₀, N₁₁₀ and N₁₆₀ treatments, respectively. Grain yield was associated with grain number m^-2, which was dependent on spikes m^-2 and grains spike^-1. Total biomass at maturity also explained the changes in yield. It is concluded that selection under stress conditions was, in this case, beneficial to identify cultivars with high yields under a wide range of nitrogen availabilities. This revised version was published online in July 2006 with corrections to the Cover Date.     

Effect of Different Preceding Crops and Crop Rotations on Yield of Winter Oil-seed Rape (Brassica napus L.)

To determine the effect of different preceding crops and crop rotations on the grain yield of oil-seed rape, a long-term rotation experiment was conducted at the Hohenschulen experimental station in Kiel, NW Germany. Additional factors included the nitrogen fertilization and the fungicide application. The results reported herein are based upon the harvest years 1988 to 1993. Averaged over the different rotations and husbandry treatments, the grain yields in the 6 experimental years varied between 2.71 t ha^?1 and 3.99 t ha^?1. In contrast, the effect of the different husbandry treatments was smaller and non significant. Averaged over 6 years, only the fungicide application caused small yield increase of 0.2 t ha^?1. The highest grain yields of 3.77 t ha^?1 or 3.65 t ha^?1 occurred when oil-seed rape was directly following peas. Low yields between 3.15 tha^?1 and 3.33 tha^?1 were obtained when oil-seed rape was grown after oilseed rape. The lowest grain yield of 3.13 t ha^?1 was produced with oil-seed rape grown in monoculture only. In rotations with oil-seed rape following a preceding cereal crop (wheat or barley), the grain yields averaged between 3.22 tha^?1 in a two course rotation and up to 3.44 tha^?1 in a four course rotation. In general, the yields of oil-seed rape increase with the length of the rotation and the length of the break between two oilseed rape crops. The yield component number of seeds per m² was affected by the previous cropping accordingly, whereas the thousand seed weight did not respond to the cropping history. Based upon disease assessments in the first years of this experiment, we argue that an increase in the incidence of fungal diseases has considerably contributed to the yield decrease of oil-seed rape in short rotations.     

Optimierung der Produktion von Wintergetreide zur Bioethanolherstellung durch unterschiedlich intensive Anbauverfahren

The investigations were based on biennial field trials carried out at two locations comprising the factors location/previous crop, winter cereal genotype (rye cv. ‘Farino’,triticale cv. ‘Modus’, wheat cv. ‘Batis’) and production intensity level. One agronomical focus was to replace the mineral N‐supply due to its energetic relevance, by either the residues of legumes, or stillage, a processing residue containing organic N. The measurement included the crop yield ha^?1, the bioethanol exploitation dt^?1 and the bioethanol yield ha^?1. The last was closely correlated to the grain yield and thus dominated by intensity level. Highest bioethanol yields with an average peak at 4022 l ha^?1, always occurred at the highest intensity level. Bioethanol exploitation however, was mainly determined by the genotype. The cultivars showed significant exploitation and yield differences. An adequate bioethanol exploitation was observed with the wheat cv. Batis in contrast to diminished grain and bioethanol yields. Considering bioethanol exploitation and bioethanol yield, the triticale cv. Modus was the outstanding genotype. Despite high grain yields, the bioethanol yields of the rye cv. Farino stayed mean, because of a genotypic lowered bioethanol exploitation. Comparing the approaches of mineral nitrogen substitution, legume N was successful, whereas stillage fertilizing, according to the examined conditions, resulted in ample decreased grain and bioethanol yields ha^?1.     

Wheat Cultivars Adapted to Post-Heading High Temperature Stress

The existence of genetic variation in wheat for tolerance to high temperature stress has been reported but cultivars released for a particular production system often are not characterized. The objective of this study was to identify and describe the characteristics of wheat cultivars adapted to production systems with risks of high temperature during the post‐heading period. Fifteen diverse wheat cultivars and one unreleased genotype were evaluated at the Texas A&M University Agricultural Research and Extension Center, Uvalde, TX, during two seasons characterized by daily maximum temperatures as high as 36 °C. Measurements during both seasons included days to heading, days to physiological maturity and grain yield. Large and significant (P < 0.05) grain yield differences were measured among cultivars within each season. Yield varied between 2979 and 4671 kg ha^?1 in the first season and between 1916 and 5200 kg ha^?1 in the second season. Late planting in the second season delayed heading date resulting in the post‐heading period to coincide with periods of high temperatures. Cultivars that headed early, in general, yielded better than cultivars that headed later within each season with yield reduction of 35.3 kg ha^?1 in the first season and 91.0 kg ha^?1 in the second season for every 1 day delay in heading after mid‐March. Early‐heading cultivars outperformed later‐heading cultivars because of two distinct advantages: the early‐heading cultivars had longer post‐heading and, therefore, longer grain filling period than the later‐heading cultivars. In addition, early‐heading cultivars completed a greater fraction of the grain filling earlier in the season when air temperatures were lower and generally more favourable. The advantage of earlier‐heading cultivars was also manifested in the amount of green leaves retained to anthesis. Earlier‐heading cultivars produced fewer total leaves per tiller but retained more green leaves and lost fewer leaves to senescence at anthesis than later‐heading cultivars. The results suggest that early heading is an important and effective single trait defining wheat cultivars adapted to production systems prone to high temperature stress during the post‐heading period.     

Genotypic Variation of Gas Exchange Parameters and Leaf Stable Carbon and Nitrogen Isotopes in Ten Quinoa Cultivars Grown under Drought

Genotypic variations in leaf gas exchange and grain yield were analysed in 10 highland‐adapted quinoa cultivars grown in the field under drought conditions. Trials took place in an arid mountain region of the Northwest of Argentina (Encalilla, Amaicha del Valle, 22°31′S, 65°59′W). Significant changes in leaf gas exchange and grain yield among cultivars were observed. Our data demonstrate that leaf stomatal conductance to water vapour (g_s) is a major determinant of net CO₂ assimilation (A_n) because quinoa cultivars with inherently higher g_s were capable of keeping higher photosynthesis rate. Aboveground dry mass and grain yield significantly varied among cultivars. Significant variations also occurred in chlorophyll, N and P content, photosynthetic nitrogen‐use efficiency (PNUE), specific leaf area (SLA), intrinsic water‐use efficiency (_iWUE) and carboxylation capacity (A_n/C_i). Many cultivars gave promissory grain yields with values higher than 2000 kg ha^?1, reaching for Sayaña cultivar 3855 kg ha^?1. Overall, these data indicate that cultivars, which showed higher photosynthesis and conductances, were also generally more productive. Carbon isotope discrimination (Δ) was positively correlated with the grain yield and negatively with _iWUE, but δ¹⁵N did not show significant correlations. This study provides a reliable measure of specific responses of quinoa cultivars to drought and it may be valuable in breeding programmes.     

Genetic gains in Nordic spring barley breeding over sixty years

Accurate assessments of genetic gains ensuing from plant breeding for the most important agronomic characteristics in Nordic spring barley (Hordeum vulgareL.) are not available. Hence this research was aimed to determine the rate of genetic improvement in the Nordic barley breeding pool. This study included 90, 2-row spring barley cultivars released (1942–1988) and29, 6-row spring barley cultivars released(1930–1991) adopted by Nordic farmers that were tested in four Nordic locations for three consecutive years. Relative genetic gain owing to plant breeding was 13% in2-row barley and 34% in 6-row barley for grain yield. The absolute gain for this characteristic was 13 ± 3 kg ha^-1 year ^-1 in 2-row barley, and22 ± 3 kg ha^-1 year^-1 in6-row barley. Improved yield was achieved in Nordic barley by reducing plant height(0.20 ± 0.04 cm year^-1 for 2-rowbarley and 0.16 ± 0.06 cm year^-1for 6-row cultivars), thereby reducing significantly lodging (0.5 ± 0.1%year^-1 and 0.4 ± 0.1year^-1), and increasing significantly the harvest index (0.0008 ± 0.0002year^-1 and 0.0018 ± 0.0002year^-1). Additionally, in 2-row spring barley cultivars resistance to powdery mildew (0.19 ± 0.08% year^-1)and thousand-kernel weight (0.07 ±0.03 g year^-1) were also significantly enhanced, whereas hectoliter weight was improved (0.06 ± 0.02 kg year^-1)in 6-row barley cultivars in the period investigated. This revised version was published online in August 2006 with corrections to the Cover Date.     

Evaluation of new breeding lines of white lupin with improved resistance to anthracnose

Since the mid-1990s, the cultivation of white lupin (Lupinus albus L.) has rapidly decreased in Germany and other countries in Central Europe because of the occurrence of Colletotrichum lupini, the causal agent of the fungal disease anthracnose, and the lack of varieties with a sufficient resistance towards this disease. To reestablish the relevance of cultivating white lupins, the development of new varieties with improved resistance is vital. In the study presented here, new breeding lines were evaluated from 2012 to 2014 on a total of five experimental sites in Germany. In each year, at least on one site a high disease pressure with good differentiation built up from natural seed infestations, so that evaluation and further selection for resistance was possible in all 3 years. The breeding lines showed improved performance of resistance towards C. lupini (anthracnose disease score 3.8 on average of all breeding lines and environments) compared to the reference varieties (anthracnose disease score 5.8). Improved resistance had a favorable effect on grain yield, particularly in environments with high disease pressure, and yield stability. Average grain yields were 2.6 t ha^?1 for the breeding lines and 1.5 t ha^?1 for the reference cultivars. Among the tested range of white lupins, the breeding material showed consistently higher grain yields on average of all environments compared to the rather low and variable yielding reference cultivars. The alkaloid content varied among environments and breeding lines.     

Management of Italian and Perennial Ryegrasses for Seed and Forage Production in Crop Rotations

Italian ryegrass (Lolium multiflorum Lam.) and perennial ryegrass (L. perenne L.) can be grown for seed and forage in cold winter regions provided the stand persists well over winter. Seed yield and plant characteristics during primary growth, and forage yield during regrowth, were determined for two Italian and one perennial ryegrass cultivars in Atlantic Canada. Establishment methods and dates included sowing ryegrass in cultivated soil alone or with barley in mid‐May and, after harvesting the barley crop, by sowing ryegrass following conventional or reduced cultivation and by no‐till drilling into barley stubble in mid‐August and early September. Despite some winterkill, particularly in Italian ryegrass, seed and forage yields were adequate in post‐establishment growing seasons. Seed yield for Italian ryegrass was greatest (1270 kg ha^?1) when it was sown into cultivated soil in mid‐August and least (890 kg ha^?1) when sown alone in May. Italian ryegrass yielded 15–17 % more seed when plots were established in mid‐August rather than in mid‐May or early September. Italian ryegrass cv. Lemtal had a greater density of fertile tillers (1030 m^?2) in the sward than cv. Ajax (860 m^?2) and its tiller density was greater when seeded into cultivated soil in September than in mid‐August. There were fewer spikelets per seed head for sowing Italian ryegrass with barley in May than for the other methods of establishment. Forage yield in regrowth was greater for Italian ryegrass cv. Ajax (2770 kg ha^?1) than for cv. Lemtal (2480 kg ha^?1). Seed yield of perennial ryegrass was greater when seeded in mid‐May than in mid‐August or early September. The seed yield of perennial ryegrass was greater when it was sown with barley in May and harvested for grain, than when it was sown alone or with barley harvested at late milk stage. The establishment methods for mid‐August and early September sowing had little effect on seed yield. However, the no‐till and reduced tillage methods resulted in a greater tiller density than sowing into the cultivated seedbed. Fertile tillers tended to be denser under reduced cultivation for sowing in August. Forage yield of perennial ryegrass regrowth was not influenced by the sowing method and timing. In conclusion, Italian and perennial ryegrasses produce adequate seed and forage regrowth under different establishment methods and timing. However, the poor persistence of Italian ryegrass may limit commercial production after the establishment year in Atlantic Canada.     

Effect of Irrigation on Winter-Sown Chickpea in a Mediterranean Environment*

In the dry Mediterranean environments of the West Asia and North Africa region, irrigation is frequently used to supplement rainfall to increase crop productivity and yield stability. Chickpea (Cicer arietinum L.), an important pulse crop of the region, often suffers from drought and can benefit from such a practice. To investigate the response of chickpea to irrigation, experiments were conducted in the field at Tel Hadya, Syria, from 1985 to 1988 using 24 improved chickpea genotypes sown in winter. Irrigation scheduling was done using the daily water balance computed from rainfall and pan evaporation data. Yearly rainfall was 316, 358, and 504 mm and supplemental irrigation amount was 130, 120, and 80 mm in 1985–86, 1986–87, and 1987–88, respectively. Irrigation increased seed yield by 916 kg ha^?1 (44.0%) over the 3-year period. Irrigation requirement for chickpea coincided with flowering and seed development period. The top 10 highest-yielding genotypes under irrigated conditions were ILC 464, ILC 1272, ILC 237, ILC 613, ILC 95, ILC 4291, ILC 142, ILC 147, ILC 295, and ILC 3256. Their mean seed yields ranged from 3877 to 3208 kg ha^?1. Among these four genotypes, ILC 464, ILC 1272, ILC 3256, and ILC 4291 with mean seed yields of 3877, 3726, 3208, and 3266 kg ha^?1, respectively, were with predictable response to favourable conditions. Aboveground biomass contributed 49% of the total increase in seed yield from irrigation followed by plant height (26%) and early maturity (16%). These results indicate that it may be possible to breed chickpea for improved response to irrigation, and irrigation can enhance the yields of winter-sown chickpea grown in the lowland Mediterranean drylands.     

Spatial variability of salt-affected soils in the middle Ebro Valley (Spain) and implications in plant breeding for increased productivity

Barley breeding programs have empirically selected for improving grain yield and quality. The objective of this study was to quantify genetic gains in yield in 2-rowed malting barley cultivars released from 1944to 1998 in Argentina, identifying the major physiological traits responsible for them. For this purpose, a field experiment was conducted in absence of biotic and abiotic stressful factors and with lodging being prevented mechanically. Until the 1970's,potential yield was maintained nearly constant at 5.25 mg ha^-1 and since then it increased at a rate of 41 kg ha^-1 year^-1. That bi-linear trend was closely related to the trend of averaged yields obtained by farmers. The contribution made by breeding yield potential to the total yield gains achieved by farmers was estimated in c. One third. Neither time to heading nor time to maturity were systematically modified by breeding. However, the partitioning of the developmental time was modified: time to achieve both maximum number of floret primordia and length of the jointing –heading period were increased with the year of release of the cultivars. The main component associated with yield was the number of grains per m², due to variations in number of spikes per m².Total and vegetative biomass at maturity increased with the year of release of the cultivars, at a rate of 45 and 19 kg ha^-1 year^-1, while both harvest index and stem height remained virtually unmodified. Differences in biomass at heading among cultivars were related to the improvement on the abilities to capture more radiation. This revised version was published online in August 2006 with corrections to the Cover Date.     

Is the Companion Crop Harmless to Alfalfa Establishment in the Highlands of East Anatolia?

This study documented the effects of barley companion crop seeding rate and cutting stage on alfalfa establishment in a highland area. Alfalfa was established with barley at seeding rates of 0, 60, 120 and 180 kg ha^?1 and cut at the milk‐dough and ripe grain stages. In most cases, hay yield and composition of herbage were affected by companion crop seeding rate and cutting stage. Total hay yield increased from 3294 to 5131 kg ha^?1 when the companion crop seeding rate was increased from 0 to 180 kg ha^?1 at the milk‐dough stage. Legume and weed growth was suppressed by the companion crop during establishment, but using a barley companion crop decreased alfalfa plant losses in the seeding year. Few residual effects of barley were seen on hay yield in the subsequent year, but residual effects of companion crop treatments on weed suppression continued in all clippings of the second year. The results suggest that alfalfa should be sown with a barley companion crop in highland areas with adequate moisture. The seeding rate for barley is about the same as that for barley grown alone, and the companion crop could be harvested for hay or grain in the establishment year.     

Analysis of adaptation of barley,triticale, durum and bread wheat under Mediterranean conditions

Summary Yield data obtained from a comparative small grain cereals trial, grown for five consecutive growing seasons at a total of 23 environments in Cyprus, were subjected to regression analysis. Within each environment, yield trials consisted of a standard set of three cultivars or elite lines of barley, triticale, durum and bread wheat. The regression coefficient (b) of crop mean on the environmental index (I) and the mean square deviation from regression (sd²) were calculated for each crop. Each crop tended to have its own characteristic value of sd² and its magnitude was an excellent indicator of specific crop-environment interaction. The causes of large sd², for two of the four crops, were the susceptibilith of barley to lodging, when favourable conditions were encountered at high yielding environments, and triticale dependence on late season precipitation. Durum wheat and triticale had an average response to different yielding environments (b>1.19) and both were significantly different from those of bread wheat (1.08) and barley (0.54). Hence, barley, bread and durum wheat are specifically adapted to low, average and high yielding Mediterranean environments, respectively. The cultivation of triticale at the expence of durum wheat is not feasible. Furthermore, interactions between crops and environments demonstrated by the regression parameters, should constitute the basis for decision making, regarding crop adaptation in a region. The average yield in all environments should not be considered as a proper criterion for adaptation. In this study, triticale had a similar mean grain yield (3,842 kg/ha) to that of bread wheat, but was significantly higher yielding than barley or durum wheat (5 and 7%, respectively).     

Maize Grain Yield Response to Tillage and Fertilizer Nitrogen Rates on a Tara Silt Loam

Effects of tillage on the appropriate fertilizer N applications needed to achieve maximal grain yield are poorly denned. The study objective was determination of relative corn grain yield response to N application rate for four tillage practices: no-tillage (NT), ridge tillage (RT), fall chisel plowing (CP) and fall moldboard plowing (MP). Maize (Zea mays L.) grain yield and N accumulation were monitored over a 6 year period with the same tillage treatment and the same fertilizer N rate applied each year to each plot. Two hybrids, differing in relative maturity rating, were planted each year. Fertilizer N rates ranged from 10 to 190 kg ha^?1 and consisted of 10 kg ha^?1 of liquid starter N applied at planting with varying amounts of fall applied anhydrous ammonia. With only starter fertilizer, grain yields increased with tillage intensity in the order NT ≤ RT ≤ CP ≤ MP. With ≥ 55 kg total applied Nha^?1, 6 year average grain yields were unaffected by tillage. Total N removed in grain annually with only starter fertilizer ranged from 25–85 kg ha^?1 Maximal amounts of N removed, about 145 kg N ha^?1, occurred with 100–145 kg applied N ha^?1 for all tillage treatments under the more favorable climatic conditions. Several interactions affecting grain yield appear climatically sensitive with exception of tillage by fertilizer N interactions. Because of variability in climate, planting dates varied by almost 4 weeks. Relative yield loss due to planting delay were Fertilizer N (mean change ??124 –?275 kg ha^?1 day^?1) > Starter N only and MP (mean ?? 259 kg ha^?1 day^?1) > other tillages in general. Yield loss due to delayed planting ranged from 0.0–275 kg ha^?1 day^?1. Grain yield gains due to early spring soil temperatures were 16.0–21.8 kg ha^?1 index-degree^?1 with MP tillage and averaged 2.7– 16.7 kg ha^?1 index-degree^?1 more than those of other tillage-hybrid combinations.     

Effects of Nitrogen and Foliar Sulphur Interaction on Grain Yield and Yield Components in Barley

In this study, conducted from 1979 to 1986 in southern Spain, the objective was to analyze the effects of a possible interaction between soil-applied N and foliar S applied to barley (Hordeum vulgare L.) during tillering on grain yield and to identify the mechanism involved. From 1979 to 1982, we used rates of 20, 40, 60 and 80 kg a.i. N ha^?1, together with 12.5 or 25 kg foliar a.i. S ha^?1 during tillering. The results demonstrated that foliar S at both dosages acted as a partial (but not total) substitute for N, when the latter was applied at levels of 40 to 60 kg ha^?1. These effects of S did not appear to result only from a nutritive mechanism, but rather from a hormonal mechanism through the increase in ethylene biosynthesis. Therefore, during 1983 to 1986, we used 40, 60 and 80 kg a. i. N ha^?1, together with 12.5 a. i. S ha^?1 and 0.55 kg a.i. ethrel (2-chloroethyl-phosphonic acid) ha^?1. The results showed that the effects of S and ethrel on yield were practically the same. Assayed with 40 and 60 kg N ha^?1, S and ethrel acted as partial (but not total) substitutes for N, exceeding the yield of the control without S or ethrel, and equalling the yield obtained with 20 kg more of N ha^?1. The S or ethrel applied with 80 kg N ha^?1 presented an additive effect with the N. The increases in yield using S or ethrel were in all cases due to the increased final number of spikes m^?2, which was principally a consequence of the higher number of tillers formed but also a result of increased survival of tillers to form a viable spike. In addition, the positive effects of S on yield were greater the smaller the N dosage and the lower the annual yield. Finally, we present a possible mechanism of hormonal action, to explain how foliar S applied during tillering affects grain yield in barley.     

Crop and Weed Growth in a Sequence of Spring Barley and Winter Wheat Crops Established Together from a Spring Sowing (Relay Cropping)

A relay cropping system of cereals, whereby winter wheat (Triticum aestivum L.) was undersown in two‐row spring barley (Hordeum distichum L.), was established in a field trial in central Sweden in 1999 and continued until 2000. The purpose of the study was to examine crop and weed responses to different plant densities of the undersown winter crop. Winter wheat was sown at four seed rates (187, 94, 47 and 0 kg ha^?1) immediately after the sowing of barley. Barley was harvested in the first autumn after sowing and winter wheat in the second autumn. The grain yield of barley was not affected by the seed rate of wheat, and averaged 4580 kg ha^?1. Winter wheat did not vernalize during the first growing season but remained at the vegetative stage. The grain yield of wheat was 1990 kg ha^?1 for the lowest and 5610 kg ha^?1 for the highest seed rate of wheat. Whilst the undersowing process itself stimulated weed emergence in this experiment, increasing the undersowing seed rate reduced the population of perennial weeds by 40–70 %. In the second growing season, the total biomass of weeds was 66 % higher at the highest seed rate compared with the lowest seed rate.     

Genotype by environment interactions (GEIs) for barley grain yield under salt stress condition

Changes in the relative genetic performance of genotypes across environments are referred to as genotype × environment interactions (GEIs). GEIs can affect barley breeding improvement for salt tolerance because it often complicates the evaluation and selection of superior genotypes. The present study evaluated the GEIs over 60 barley genotypes for yield components and grain yield in six salinity environments in North Delta, Egypt. Data were analyzed using the additive main effects and multiplicative interaction (AMMI) and Tai’s stability parameters. GEIs effects on yield explained 20.3, 20.1, 14.6, and 33.0% of the total variation besides, the first two principal components account for 67.3, 56.3, 64.3, and 83.7% of the explained variance in the four sets, respectively. Six genotypes namely G-4, G-7, G-20, G-34, G-36, and G-39 were found to be most stable and high yielding across environments (GY >2.00 t ha^-1), and located close to zero projection onto the AEC ordinate. Tai’s stability parameters demonstrated that these genotypes were more responsive to the environmental changes. The genotypes G-50 and G-53 showed perfect/static stability (α = -0.95, -0.91, respectively). In contrast, the genotype; G-36 had α = 0 and λ = 1.10, indicating parallel with the environmental effects followed by G-44. Overall, we found that GEIs for grain yield are highly significant in all sets, suggesting that responded differently across environments. This interaction may be a result of changes in genotypes’ relative performance across environments, due to their differential responses to various abiotic factors.