Effect of nitrogen supply on leaf appearance,leaf growth,leaf nitrogen economy and photosynthetic capacity in maize (Zea mays L.)

Leaf area growth and nitrogen concentration per unit leaf area, N_a (g m⁻² N) are two options plants can use to adapt to nitrogen limitation. Previous work indicated that potato (Solanum tuberosum L.) adapts the size of leaves to maintain N_a and photosynthetic capacity per unit leaf area. This paper reports on the effect of N limitation on leaf area production and photosynthetic capacity in maize, a C4 cereal. Maize was grown in two experiments in pots in glasshouses with three (0.84–6.0 g N pot⁻¹) and five rates (0.5–6.0 g pot⁻¹) of N. Leaf tip and ligule appearance were monitored and final individual leaf area was determined. Changes with leaf age in leaf area, leaf N content and light-saturated photosynthetic capacity, P_max, were measured on two leaves per plant in each experiment. The final area of the largest leaf and total plant leaf area differed by 16 and 29% from the lowest to highest N supply, but leaf appearance rate and the duration of leaf expansion were unaffected. The N concentration of expanding leaves (N_a or %N in dry matter) differed by at least a factor 2 from the lowest to highest N supply. A hyperbolic function described the relation between P_max and N_a. The results confirm the ‘maize strategy’: leaf N content, photosynthetic capacity, and ultimately radiation use efficiency is more sensitive to nitrogen limitation than are leaf area expansion and light interception. The generality of the findings is discussed and it is suggested that at canopy level species showing the ‘potato strategy’ can be recognized from little effect of nitrogen supply on radiation use efficiency, while the reverse is true for species showing the ‘maize strategy’ for adaptation to N limitation.     

Effect of nitrogen source on growth and morphogenesis of three micropropagated potato cultivars

Summary Different nitrogen sources (NO₃ ⁻, NH₄ ⁺, glutamic acid and their combinations) influenced the growth and morphogenic responses (node number, shoot length, and stem, leaf and root dry weight) of three micropropagated potato cultivars (Spunta, Kennebec, Huinkul). Addition of reduced nitrogen (NH₄ ⁺ or glutamic acid) in a nitrate medium increased shoot length and leaf number. The large increase in growth in plants fed with NO₃ ⁻, NH₄ ⁺ could be explained by higher organic nitrogen content and enhanced dry matter partition to the shoot. This suggests that reduced nitrogen source is required, at least as a supplement to NO₃ ⁻, to enhance N assimilation and growth.     

The effects of nitrogen supply and stem density on leaf attributes and stem branching in potato (Solanum tuberosum L.)

Summary The interaction between nitrogen supply and stem density on leaf attributes and branching was studied in two field experiments. Stem densities included 8, 16, 24, 32 and 40 stems m⁻². The low-N treatments received 5 or 10 g m⁻² nitrogen, and the high-N treatments 20 or 25 g m⁻². The sizes of mature individual leaves on the main stem declined with increase in stem density and were larger for greater N supply, with additive effects of nitrogen and stem density. The specific leaf area was not affected by nitrogen supply and increased with stem density (range of values: 210 to 310 cm² g⁻¹). The leaf weight ratio was not affected by the treatments. At 8 and 16 stems m⁻² basal branches contributed substantially to the total leaf area per stem: at 24 stems m⁻² and higher densities the development of basal branches was negligible. Leaf area on apical branches was always smaller for higher plant densities and lower rates of N supply.     

The influence of leaf area,light interception and season on potato growth and yield

Summary Light interception in the photosynthetically active radiation (PAR) range (400 700 nm) was measured over two years in potato crop canopies. Light interception increased linearly with increasing leaf area index (L) up to values of 2.25, light interception increased at a decreasing rate until L=4.0 was attained when around 95% of the incoming radiation was intercepted. The equation I_l=I_oe^kl accounted for 92% of the variance in the relationship between light interception and L, where I_l is the irradiance on a horizontal plane below a leaf area index of L., and k (light extinction co-efficient)=0.335+0.151 (L) 0.0129 (L)². Crop growth rate was found to be linearly related to the PAR intercepted by the canopy in 1980, in the absence of any apparent water-stress. The photosynthetic conversion efficiency of the canopy (g dry weight MJⁱ PAR intercepted) was 3.42 and 2.49 for 1980 and 1979, respectively. Tuber yields were also found to be linearly related to PAR intercepted by the canopy over the whole season.

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Zusammenfassung In den Jahren 1979 und 1980 wurde die Lichtaufnahme der photosynthetisch aktiven Strahlung (PAR) im Bereich von 400–700 nm in Kartoffelbest?nden im Vereinigten K?nigreich gemessen. Die meteorologischen Daten zeigten, dass der Niederschlag in den Jahren sehr unterschiedlich war (Abb. 2); 1979 war überwiegend trocken, vor allem in der sp?teren Vegetationszeit, w?hrend 1980 allgemein ein nasses Jahr war. Die Lichtaufnahme stieg linear mit dem zunehmenden Blattfl?chenindex (L) bis zu L=2,25 an (wobei rund 73% der einfallenden Strahlung aufgenommen wurde) und über L=2,25 nahm die Lichtaufnahme mit einer abnehmenden Rate bis zu L=4,0 zu, wobei rund 95% der einfallenden Strahlung aufgenommen wurden (Abb. 3). Die Gleichung I_L=I_oe^kL gilt für 92% der Varianz in der Beziehung zwischen Lichtaufnahme und Blattfl?chenindex (L), wobei I_L=Strahlung auf einer horizontalen Fl?che unter einem Blattfl?chenindex von L und k (Lichtextinktionskoeffizient)=0,335+0,151(L)−0,0129(L)². Wenn die Konstante k (=0,72) verwendet wurde (Abb. 3) entsprach die Varianz für das ausgew?hlte Modell 88%. Zwischen dem Gesamttrockengewicht (TDW) und der PAR, die aufgenommen wurde, bestand in beiden Jahren ein signifikantes lineares Verh?ltnis. Abb. 4 zeigt die Daten für 1980. Durch den Nullpunkt geführt ergibt sich ein TDW=3,42 (PAR) und 2,49 (PAR) für 1980 bzw. 1979, das bedeutet, dass die Umwandlung von Lichtenergie in Trockensubstanz ohne ersichtlichen Wassermangel 1980 wirkungsvoller war. Abb. 5 zeigt die photosynthetische Wirksamkeit (g Trockengewicht MJ^l PAR) w?hrend der Vegetationszeiten zusammen mit den ?nderungen im Blattfl?chenindex L. Die Wachstumsrate des Bestandes stand 1980 in einem linearen Verh?ltnis zu der von den Bl?ttern aufgenommenen PAR, w?hrend sich für 1979, wo der Bestand stark unter Trockenheit litt, eine Abweichung ergab (Abb. 6). Obwohl w?hrend der beiden Jahre die photosynthetische Wirksamkeit (g Knollentrockengewicht MJ^l PAR, aufgenommen w?hrend der gesamten Vegetationszeit) für das Knollengewicht unterschiedlich war: Knollentrockengewicht=1,97 (PAR) und 2,37 (PAR) für 1979 bzw. 1980, zeigen die Daten, dass ein gesteigertes Wachstum entsprechend einer h?heren Lichtaufnahme zu h?heren Knollenertr?gen führt, unabh?ngig von einer Reihe ackerbaulicher Massnahmen.

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Résumé L'interception des radiations actives pour la photosynthèse (PAR), dans des longueurs d'onde de 400 à 700 nm, est mesurée durant très différente les deux années; 1979 fut particulièrement sec, surtout en fin de saison, alors que 1980 fut une année humide. La quantité de lumière interceptée, augmente linéairement avec l'index de surface foliaire (leaf area index) jusqu'à une valeur de L=4,25 (correspondant à 73% de radiations interceptées) et pour des valeurs supérieures, l'interception augmente à une vitesse décroissante jusqu'à L=4,0 correspondant à 95% de radiations captées (fig. 3). La relation I_L=I_oe^kL interpréte 92% de la variation dans la liaison entre la lumière interceptée et l'index de surface foliaire (L), où I_L est le rayonnement atteignant un plan horizontal situé sous un index foliaire L et K (coefficient d'extinction lumineuse)=0,335+0,151(L) −0,0129(L)². Si on fixe k à 0,72 (fig. 3), la part de variation expliquée par le modèle est de 88%. Une relation linéaire significative entre la matière sèche totale (TDW) cumulée et le PAR intercepté cumulé, a été établie pour les deux années et les données de 1980 sont portées dans la figure 4. Si on fait passer la régression par l'origine, on écrira pour 1979: TDW=2,49× deux années, 1979 et 1980, dans des cultures de pomme de terre du Royaume-Uni. Selon les données météorologiques, la pluviométrie fut et pour 1980: TDW=3,42×PAR. Ces relations indiquent que la conversion de lumière en matière sèche a été plus efficace en 1980 en l'absence de déficit hydrique. L'efficacité de la conversion photosynthétique (g de matière sèche/PAR intercepté) au cours de la saison, est indiquée avec les variations de L dans la figure 5. La vitesse de croissance de la culture est liée linéairement au PAR capté par la végétation en 1980, alors que cette liaison s'écarte de la linéarité en 1979, lorsque la culture est affectée par la sécheresse (fig. 6). Bien que l'efficacité de la conversion photosynthétique (g de poids sec de tubercule/PAR captée durant toute la saison) pour le poids de tubercules soit légèrement différente pour les deux années (en 1979 elle est égale à 2,37 (PAR), en 1980 elle est égale à 1,97 (PAR)). Les données indiquent que l'augmentation de la croissance de la récolte totale, expliquée par l'augmentation de la lumière captée, résultent également du rendement de plus en plus grand des tubercules (fig. 7), sur une série de traitements agronomiques.

     

Influence of nitrogen supply on micropropagation and subsequent microtuberization of four potato cullwars

A medium containing low amounts of nitrogen (19–23 meq.l^?1) produced optimum results in micropropagation as revealed by the number of nodes, internode length, chlorophyll content, and leaf area of four potato cvs. belonging each to four different maturity groups. Decreasing amounts of nitrogen also increased chlorophyll content in all four cultivars tested. The NH ₄ ⁺ concentration did not have an effect on micropropagation for low nitrogen supplies. In all cvs., except Baraka, there was a “carry over” effect of the nitrogen content in the micropropagation medium onto subsequent tuberization, the lower nitrogen (23 meq.l^?1) advancing tuber initiation. Microtuberization of cv. Jaerla was earlier in darkness than under short days regardless of the propagation medium used.     

Effect of leaf age on photosynthesis,carbon transport and carbon allocation in potato plants

Summary Photosynthetic characteristics, carbon allocation and partitioning of newly-fixed¹⁴C among the various carbohydrate fractions were studied in leaves of potato plants (Solanum tuberosum L. cv. Désirée) of different ages. The higher photosynthetic rate of young leaves was associated with higher levels of starch and reducing sugars. After 24 hours, in young leaves the fixed¹⁴C had been partitioned mainly to the residue and starch fractions, while in older leaves there was an increase in partitioning to sucrose and reducing sugars. The activities of the various sink organs were found to be dependent upon their distance from the source leaf. Tuber activity, however, was affected similarly by all fully expanded leaves. Although young fully expanded leaves are thought to be the major source of assimilates for tuber growth, it seems that a vast potential for contributing to plant productivity and tuber yield lies also in the older leaves, which translocate most of their photoassimilates to the tubers.     

鸡脚叶对陆地棉光合特性的影响

用远缘杂交后代为材料,测定了不同叶型不同时期的叶绿素含量、光合强度.结果表明:在吐絮期前鸡脚叶叶片的叶绿素含量和光合作用强度均高于正常叶.而在吐絮期后正好相反.考查了不同叶型的单株结铃数、铃重、衣分、虫籽率和僵瓣率.试验结果表明:鸡脚叶对陆地棉产量没有不利影响;与正常叶比较,红铃虫为害的虫籽率和僵瓣率分别低32.7%和18.8%.     

Effect of potato leaf roll virus on autumn potatoes in Israel

Summary In field trials simulating commercial conditions, secondary PLRV infection of autumn-grown potato crops in Israel significantly reduced yield, although symptoms were not perceptible. Plots containing 7.3% PLRV-infected seed showed an increase in yield per plant of 17%, compared with plots with 36.2% infected seed; in plots with only 1.3% infected seed the increase of yield was 21%. In a second experiment, where individual plants were assayed, the yield of PLRV-infected plants of cv. Blanka was only 68.7% of that of PLRV-free plants; corresponding figures for cv. Up-to-Date and Désirée were 79.2 and 58% respectively. PLRV infection was not associated with reduced emergence or with the yield of deformed tubers.

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Zusammenfassung W?hrend der ersten zwei Monate der im Herbst liegenden Wachstumszeit der Kartoffel (August-Januar) sind in Israel sehr hohe Temperaturen (mittleres Maximum 34,7° C) und hohe Sonneneinstrahlung; anschliessend fallen die Temperaturen und die Lichteinstrahlung stark ab. Unter diesen Bedingungen bleibt die Infektion mit PLRV meist symptomlos und es gibt vom Pflanzenwuchs her keine Anzeichen dafür anzunehmen, dass der Ertrag beeintr?chtigt ist. Imersten Versuch (A) wurden die Ertr?ge von Best?nden verglichen, die die gleiche Pflanzgutgr?sse (Sorte Désirée) hatten, aber von drei Herkünften I, II und III stammten. Der Anteil der PLRV infizierten Knollen in diesen Herkünften betrug 1,3, 7,8 und 32,6% (Abb. 1A). Der durchschnittliche Ertrag pro Parzelle für Herkunft I, II und III lag bei 12,6 kg, 10,0 kg und 7,6 kg (Abb. 1C); die Differenzen zwischen den Herkünften waren signifikant (P<0,05). Der Unterschied zwischen Herkunft I und II gibt vielleicht die unterschiedlichen Auflaufraten wieder (Abb. 1B), aber die Differenz zwischen den Herkünften II und III scheint eine Folge der Virusinfektion zu sein, und ?hnliche Differenzen wurden beim Vergleich des Ertrages pro Pflanze gefunden (Abb. 1D). Diese Annahme wurde im Versuch B best?tigt, in dem der Ertrag PLRV-infizierter Pflanzen mit dem PLRV-freier Pflanzen verglichen wurde. Die Infektion mit PLRV war nicht mit einer verringerten Auflaufrate oder dem Ertrag an deformierten Knollen verbunden, aber infiziertes Pflanzgut der Sorte Blanka ergab nur 68,7% des Ertrages virusfreien Pflanzgutes, Up-to-Date 79,2% und Désirée 58%; die Verluste waren statistisch signifikant (Abb. 3). Diese Ergebnisse lassen annehmen, dass betr?chliche Ertragsverluste durch die Infektion mit PLRV in den im Herbst gepflanzten Kartoffeln eintreten k?nnen, obwohl die Symptome nicht wahrnehmbar sind. Im Hinblick auf den hohen PLRV-Befall in den Herbstbest?nden (Marco, 1981b), kann es von Vorteil sein die im Frühjahr gepflanzten Best?nde vor einer PLRV-Infektion zu schützen, da sie das Pflanzgut für den Herbst stellen.

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Résumé Pendant les deux premiers mois de végétation des pommes de terre cultivées à l'automne en Israel (Ao?t-Janvier), les températures sont très élevées (moyenne maximale de 34,7°C) et la luminosité très forte. Puis les températures et l'intensité lumineuse diminuent rapidement. Sous de telles conditions, la contamination par le virus de l'enroulement est généralement existante mais sans l'apparition de sympt?mes; rien ne permet donc d'indiquer en végétation que le rendement est affecté. Dans une première expérience (A), le rendement est comparé pour trois cultures obtenues à partir de plants de même calibre (variété Désirée), mais de sources différentes (I, II et III). Suivant l'origine I, II et III les pourcentages de la contamination par le virus de l'enroulement est respectivement 1,3, 7,8 et 32,6% (fig. 1A). Le rendement moyen obtenu par parcelle est respectivement 12,6, 10 et 7,6 kg (fig. 1C), la différence entre les trois origines étant significative (P<0,05). L'écart de rendement entre les plants de source I et II s'explique sans doute par le taux de levée différent (fig. 1B); mais entre les plants de source II et III, la différence semble provenir de la contamination par le virus et ceci est également observé pour le rendement par pied (fig. 1D). Cette hypothèse est confirmée par l'expérience B ou le rendement obtenu à partir de plants contaminés est comparé à celui du témoin planté avec des tubercules indemnes de virus. L'incidence de la contamination n'est pas correlée au manque à la levée et au rendement des tubercules difformes. Les plants contaminés ont donné pour les variétés Blanka, Up-to-Date et Désirée respectivement 68,7%, 79,2% et 58% du rendement obtenu à partir de tubercules sains, la diminution de rendement étant significative (fig. 3). Les résultats montrent qu'il peut y avoir des

     

Effect of supplemental nitrogen on true potato seed weight

The effect on true potato seed (TPS) weight of supplemental nitrogen (N) applied during seed development was investigated using crosses DTO-33 × R128.6 (“A” produced in the field) and Atzimba × R128.6 (“B” produced in the field and in a screenhouse). Dry weights of tops and tubers of the mother plants were also measured in the screenhouse. The response to supplemental N (0-240 kg/ha) in 100-TPS weight of cross A and B from the field was positive and linear. In the screenhouse, where higher total N (0-1200 kg/ha) was applied, the responses in 100-TPS weight and dry weight of tops and tubers were curvilinear, with maximum levels at 800, 1000 and 400 kg/ha, respectively. The 100-TPS weight of cross B was 40% higher in the field than in the screenhouse. In the field, increased frequency of supplemental N applications increased 100-TPS weight of large and medium berries of cross B, but had no effect on seed from small berries nor on seed from any berries of cross A. In the screenhouse, increased application frequency decreased tuber dry weight and increased dry weight of tops, but had no effect on 100-TPS weight. It was concluded that supplemental N must be applied during seed development and at higher total levels than those required for optimum tuber yields in order to maximize 100-TPS weight. The lower seed weight from the screenhouse suggests that other environmental factors (e.g., temperature) present during growth of the mother plant can affect the weight of the resultant TPS.     

ABT生根粉对花生根系活力及叶片光合性能的影响

在田间栽培条件下,研究花生结荚后期叶面喷施ＡＢＴ生根粉对促进花生后期根系活力及叶片光合性能的作用。研究结果表明,应用ＡＢＴ生根粉明显提高花生根系活力,增加后期功能叶片的叶绿素含量,使叶片保持较高的光合速率。ＡＢＴ２００ｍｇ／Ｌ处理综合效果较好,在生产上可作为促进花生生育后期生发育的一项技术措施。     

Effect of nitrogen supply on crop conductance,water- and radiation-use efficiency of wheat

Water-use efficiency (WUE_DM) is directly related to radiation-use efficiency (RUE) and inversely related to crop conductance (g_c). We propose that reduced WUE_DM caused by shortage of nitrogen results from a reduction in RUE proportionally greater than the fall in conductance. This hypothesis was tested in irrigated wheat crops grown with contrasting nitrogen supply; treatments were 0, 80 and 120 kg N ha⁻¹ in 1998 and 0, 80, 120 and 160 kg N ha⁻¹ in 1999. We measured shoot dry matter, yield, intercepted solar radiation and soil water balance components. From these measurements, we derived actual evapotranspiration (ET), soil evaporation and transpiration, WUE_DM (slope of the regression between dry matter and ET), WUE_Y (ratio between grain yield and ET), RUE (slope of the regression between dry matter and intercepted radiation), and g_c (slope of the regression between transpiration and intercepted radiation). Yield increased from 2.3 in unfertilised to an average 4.7 t ha⁻¹ in fertilised crops, seasonal ET from 311 to 387 mm, WUE_DM from 23 to 37 kg ha⁻¹ mm⁻¹, WUE_Y from 7.6 to 12.4 kg ha⁻¹ mm⁻¹, RUE from 0.85 to 1.07 g MJ⁻¹, while the fraction of ET accounted for soil evaporation decreased from 0.20 to 0.11. In agreement with our hypothesis, RUE accounted for 60% of the variation in WUE_DM, whereas crop conductance was largely unaffected by nitrogen supply. A greater fraction of evapotranspiration lost as soil evaporation also contributed to the lower WUE_DM of unfertilised crops.     

Effect of inorganic nitrogen nutrition on cytokinin-induced potato microtuber production in vitro

Summary The effect of inorganic nitrogen nutrition on the induction and development of microtubers by cytokinin-induced tuberization was studied in four potato genotypes belonging to different maturity groups. The objective of this study was to investigate whether a reduction in total nitrogen level in the Murashige & Skoog medium would improve cytokinin-induced tuberization rate. The effect of three levels of total nitrogen (15, 30 and 45 meq) on tuberization was studied at constant (20 meq K) and varying potassium levels approximating to 5, 10 and 15 meq. Reducing the total nitrogen supply increased the number but decreased the size of nitrogen level on the rate of assimilate partitioning (harvest index) during cytokinin-induced microtuberization.     

Effect of seedpiece spacing and nitrogen fertilization on tuber yield, yield components, and nitrogen use efficiency parameters of two potato cultivars

The effect of seedpiece spacing on the efficiency of nitrogen (N) use by the potato crop is generally unknown. The objective of this experiment was to determine the effect of seedpiece spacing on tuber yield, yield components and N use efficiency parameters of two potato cultivars. Potato cultivars Atlantic and Shepody were grown at two rates of N fertilization (0 or 100 kg N ha^?1) and three seedpiece spacings (20, 30, or 40 cm) in 2000 to 2002. Wider seedpiece spacing increased mean tuber weight and the number of tubers per stem, but decreased total tuber yield. The higher tuber yield at the narrow seedpiece spacing was attributed to higher biomass production in combination with lower tuber specific gravity. Seedpiece spacing had no consistent effect on plant N accumulation, and therefore no consistent effect on N uptake efficiency (plant N accumulation /N supply from the soil plus fertilizer). However, a small increase in soil NO₃-N concentration in the hill at topkill at wider seedpiece spacing suggested plant N accumulation was slightly reduced at wider seedpiece spacing, but at a level that could not be detected from a plant-based measure of N accumulation. The reduced dry matter accumulation, but similar plant N accumulation, resulted in lower N use efficiency (plant dry matter accumulation / N supply) at wider seedpiece spacing. Wider seedpiece spacing also resulted in generally lower values of N utilization efficiency (plant dry matter accumulation / plant N accumulation) for the 40-cm compared with the 20- and 30-cm seedpiece spacings. Effects of seedpiece spacing on N use efficiency parameters were generally consistent across cultivars and fertilizer N rates. Wider seedpiece spacing did reduce the efficiency of N use by the potato crop; however, the magnitude of the effect was small under the conditions of this study.     

Accumulation of protein,non-protein nitrogen and starch during tuber growth of three potato cultivars

The proportion of protein and non-protein nitrogen (NPN) in tuber dry matter decreases rapidly during the initial stages of tuber growth. Protein then stabilizes but NPN increases during the final stages of tuber growth. The percentage of starch in the dry matter increases rapidly during early tuber growth and then either stabilizes or increases at a slower rate. Protein and starch are accumulated at constant rates with respect to tuber growth and the relative amount of protein or starch in the tuber is logarithmically related to tuber size. During tuber growth some protein molecular weight classes increase in concentration while others decrease. We propose a model, based on the growth of storage parenchyma, to explain the data.     

减氮配施钙肥对花生光合特性、产量及肥料贡献率的影响

长期大量施用化肥造成土壤退化、环境污染等诸多问题。为培肥地力、提高肥料利用率,于2018年和 2019年在山东省沂南县设置减氮配施钙肥试验,选用品种龙花5号为试材,探明氮钙运筹对花生光合特性、产量、肥 料贡献率的影响。结果表明：与常规施氮量相较,减氮25%和35%处理降低了花生的主茎高、叶面积指数、净光合 速率、百果重、百仁重和产量,但对出米率无显著影响。氮肥不变的前提下,增施钙肥可促进花生主茎高的生长,增 加花生叶面积指数、SPAD、净光合速率和产量。氮钙运筹情况下,减氮25%配施300 kg∙hm-2 钙肥处理的净光合速 率、叶面积指数、产量、肥料贡献率最高。通过数学建模分析得出,2018年减氮25%配施311.15 kg∙hm-2 钙肥时花生 产量最高,2019年减氮25%配施304.99 kg∙hm-2钙肥时花生产量最高。本试验为花生均衡施肥、高产、稳产提供理 论支撑。     

Effect of hypovirulent rhizoctonia solani on rhizoctonia disease,growth, and development of potato plants

Field experiments were conducted to determine whether a hypovirulent, anastomosis group (AG) 3 isolate (Rhs 1A1) of R. solani could control rhizoctonia disease of potato caused by a virulent AG-3 isolate (Rhs 27). A uniform amount of Rhs 1A1, Rhs 27, or both was applied to formaldehyde-disinfected potato seed tubers at planting. In plots inoculated with Rhs 1A1 and Rhs 27, the area of infected stem tissue was significantly (P = 0.05) reduced by 56% as compared with that of plots inoculated with Rhs 27 alone. The number of stolons per plant was decreased by isolate Rhs 27 to one-half that of the uninoculated control, but remained unchanged in plots treated with both Rhs 1A1 and Rhs 27. The type of inoculum did not affect the percentage of infected stems or stolons. Plants inoculated with Rhs 1A1 alone exhibited a significantly (P = 0.05) greater growth response, expressed as a 4-fold increase in the dry weight of stolons, a 1.7-fold increase in the dry weight of stems, and reached full bloom approximately one week earlier than their respective control. Tuber yield increases associated with Rhs 1Al-treated plots were not statistically significant. When untreated seed tubers bearing natural inoculum (sclerotia) of R. solani were used, Rhs 1A1 failed to reduce disease severity or sig-nificantly stimulate plant growth.