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HPLC检测分析速溶绿茶游离氨基酸
引用本文:朱旗,施兆鹏,童京汉,任春梅. HPLC检测分析速溶绿茶游离氨基酸[J]. 茶叶科学, 2001, 21(2): 134-136. DOI: 10.13305/j.cnki.jts.2001.02.013
作者姓名:朱旗  施兆鹏  童京汉  任春梅
作者单位:湖南农业大学食科院,湖南农业大学食科院,中国茶叶进出口公司,湖南农业大学理学院 湖南长沙410128 ,湖南长沙410128 ,北京100001 ,湖南长沙410128
基金项目:国家重点科技项目 (攻关 )计划 85_51 31_2 3_0 6
摘    要:本文采用AccQ .Tag衍生方法经HPLC检测分析茶叶的游离氨基酸 ,结果表明 ,该方法能够较好地分离氨基酸组分 ,色谱图形较好。在绿茶原料中检测出 16种游离氨基酸 ,其中茶氨酸的含量最高 ,占氨基酸总量的 5 6 0 3 %,其次是谷氨酸、天冬氨酸、丝氨酸和蛋氨酸等。在速溶绿茶中同样检测出 16种相同的游离氨基酸 ,但氨基酸总量及各氨基酸组分的相对含量发生了不同程度的变化

关 键 词:HPLC  速溶绿茶  氨基酸  AccQ.Tag法  
文章编号:1000-369X(2001)02-0134-03
修稿时间:2001-04-15

HPLC Determination of Free Amino Acids In Instant Green Tea
ZHU Qi ,SHI Zhao_peng ,TONG Jing_han ,REN Chun_mei. HPLC Determination of Free Amino Acids In Instant Green Tea[J]. Journal of Tea Science, 2001, 21(2): 134-136. DOI: 10.13305/j.cnki.jts.2001.02.013
Authors:ZHU Qi   SHI Zhao_peng   TONG Jing_han   REN Chun_mei
Affiliation:ZHU Qi 1,SHI Zhao_peng 1,TONG Jing_han 2,REN Chun_mei 3
Abstract:The free amino acids of green tea were determined by the AccQ.Tag derivation and HPLC method. The results showed that this method can separate the components of amino acids very well and get a very good HPLC chromatogram. 16 free amino acids of green tea were tested. Theanie had the highest content among them, reaching 56 03% of the total. The next were Glutamic acid, Aspartic acid, Serine, Methionine ect. The same 16 amino acids were tested in instant green tea, but the total content and the constitute of components changed differently.
Keywords:HPLC  Instant green tea  Amino acids  AccQ.Tag method
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