Pharmacokinetics of florfenicol and behaviour of its metabolite florfenicol amine in orange‐spotted grouper (Epinephelus coioides) after oral administration |
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| Authors: | J‐B Feng D‐R Huang M Zhong P Liu J‐D Dong |
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| Affiliation: | 1. Key Laboratory of Tropical Marine Bio‐resources and Ecology (LMB), Key Laboratory of Applied Marine Biology of Guangdong Province and Chinese Academy of Sciences (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China;2. Tropical Marine Biological Research Station in Hainan, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Sanya, China;3. School of Pharmaceutical Sciences, South Medical University, Guangzhou, China;4. Department of Aquaculture, College of Animal Science, South China Agricultural University, Guangzhou, China |
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| Abstract: | Pharmacokinetics and elimination of florfenicol and florfenicol amine in grouper held in sea water at 23.3 ± 0.8 °C were studied using HPLC method after they were given a single peroral dose of florfenicol at 24 mg kg?1 body weight. Florfenicol was rapidly absorbed from intestine and distributed extensively to all the tissues examined. The maximum concentrations (Cmax, μg g?1 or μg mL?1) in plasma and tissues were observed at 2–6 h (the time to reach maximum concentration, Tmax) except for bile (Tmax = 24 h) and were in the order of intestine (52.02 ± 25.07) > bile (49.41 ± 28.16) > gill (45.12 ± 11.10) > plasma (28.28 ± 5.43) > liver (21.97 ± 12.08) > muscle (21.63 ± 6.12) > kidney (20.88 ± 11.28) > skin (19.10 ± 5.88). The drug distribution level was higher in plasma than in extravascular tissues except for bile, based on the ratios of the area under concentration–time curve between tissue and plasma (AUCtissue/plasma). The elimination of florfenicol was rapid in fish, and the corresponding half‐lives (T1/2β) in the order of magnitude were bile (13.92 h) > muscle or liver (12.31 h) > skin (11.77 h) > plasma (11.57) > gill (11.04 h) > intestine (10.55 h) > kidney (10.05 h). The delayed Tmax, lower Cmax and longer T1/2β for florfenicol amine compared with florfenicol were measured in grouper. |
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| Keywords: | distribution elimination florfenicol florfenicol amine grouper pharmacokinetics |
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