| 甜叶菊绿原酸增强大肠杆菌感染蛋雏鸡免疫力研究 |
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| 引用本文： | 卓春柳,王方方,钟翠红,王斌,石玉祥,徐美利,武亚南,张永英. 甜叶菊绿原酸增强大肠杆菌感染蛋雏鸡免疫力研究[J]. 畜牧兽医学报, 2021, 52(8): 2200-2212. DOI: 10.11843/j.issn.0366-6964.2021.08.013 |
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| 作者姓名： | 卓春柳 王方方 钟翠红 王斌 石玉祥 徐美利 武亚南 张永英 |
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| 作者单位： | 1. 河北工程大学, 邯郸 056000;2. 晨光生物科技集团股份有限公司, 邯郸 056000 |
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| 基金项目： | 河北省科技厅立项项目（19046637Z）；河北省植物天然色素产业研究院（20181114） |
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| 摘 要： | 旨在评价甜叶菊绿原酸增强人工腹气囊感染大肠杆菌O78蛋雏鸡免疫力的作用效果,为功能性抗生素替代品研发提供基础参数支持.本试验随机将1日龄、体重无显著差异的健康海兰蛋鸡360只分为6组:空白对照组(C)、大肠杆菌O78处理组(EC0)、1.0g·L-1杜仲素+大肠杆菌O78处理组(ED1)、1.0g·L-1甜叶菊绿原酸+...
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| 关 键 词： | 蛋雏鸡 绿原酸 大肠杆菌O78 腹气囊感染 免疫功能 |
| 收稿时间： | 2020-12-28 |
Effects of Stevia Chlorogenic Acid on Enhancing the Immunity of E.coli O78-infected Layers |
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| ZHUO Chunliu,WANG Fangfang,ZHONG Cuihong,WANG Bin,SHI Yuxiang,XU Meili,WU Ya'nan,ZHANG Yongying. Effects of Stevia Chlorogenic Acid on Enhancing the Immunity of E.coli O78-infected Layers[J]. Chinese Journal of Animal and Veterinary Sciences, 2021, 52(8): 2200-2212. DOI: 10.11843/j.issn.0366-6964.2021.08.013 |
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| Authors: | ZHUO Chunliu WANG Fangfang ZHONG Cuihong WANG Bin SHI Yuxiang XU Meili WU Ya'nan ZHANG Yongying |
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| Affiliation: | 1. Hebei University of Engineering, Handan 056000, China;2. Chenguang Biotech Group Co. Ltd., Handan 056000, China |
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| Abstract: | This study aimed to evaluate the effect of stevia chlorogenic acid on enhancing immunity of layers infected with E.coli O78 via abdominal air sac artificially, and to provide basic parameter support for the development of functional antibiotic substitutes. A total of 360 1-day-old healthy Hailan layers with no significant difference in body weight were randomly divided into 6 groups:control group (C), E.coli O78 treatment group (EC0), 1.0 g·L-1 eucommdin + E.coli O78 treatment group (ED1), 1.0 g·L-1 stevia chlorogenic acid + E.coli O78 treatment group (EC1), 2.0 g·L-1 stevia chlorogenic acid + E.coli O78 treatment group (EC2), 4.0 g·L-1 stevia chlorogenic acid + E.coli O78 treatment group (EC4), the formal test started after 7 days of pre-feeding. On the 7th day, the layers was infected with E.coli O78through the abdominal air sac. The treatment lasted for 3 days, and medicine was dissolved into drinking water once a day. Subsequently, the serum levels of IL-1β, IL-2, IL-6, IgM, IgA and TNF-α were detected by ELISA; the expression level of IL-1β, IL-2, TNF-α, Claudin-1 and ZO-1 genes in jejunum and ileum were detected by RT-qPCR; the high-throughput sequencing was used to analyze the types of microorganisms in the cecum contents.The results showed that:1) E.coli O78 significantly increased the mortality of layers (P<0.05), while the mortality of layers in EC2 and EC4 groups were significantly reduced (P<0.05). 2) Stevia chlorogenic acid had a tendency to increase serum IgA and IgM levels in E.coli O78-infected layers, and reduce serum inflammatory factor levels to varying degrees. Among them, serum TNF-α levels of layers in EC1, EC2 and EC4 groups were significantly reduced (P<0.05); the gene expression of ileal pro-inflammatory cytokines IL-1β, IL-2 and TNF-α in layers infected with E.coli O78 were significantly reduced in EC2 group (P<0.05). 3) Stevia chlorogenic acid could promote the expression of jejunum tight junction protein gene, and improve the intestinal barrier damage caused by E.coli O78 infection. 4) E.coli O78 infection would lead to the increase of specific OTUs in intestine of layers, and affect the composition of cecum microflora. The relative abundance of Bacteroidetes, Proteobacteria and Fusobacteria were increased and the relative abundance of Firmicutes was decreased by the injection of E.coli O78. However, stevia chlorogenic acid treatment group (EC2) could improve the relative abundance of Firmichoides in the cecum and reduce the relative abundance of Bacteroidetes and Proteobacteria in layers infected with E. coli. Stevia chlorogenic acid can enhance the immune function of layers infected with E.coli O78 in the abdominal air sacs, and resist the invasion of E.coli O78 to layers. The application dose of 2.0 g·L-1 stevia chlorogenic acid has a better effect. This indicates that chlorogenic acid has the effect of an antibiotic substitute. Its positive effect on the immunity of E.coli-infected layers may be achieved by regulating immune-related genes and maintaining the steady state of the cecal microbial flora, but its mechanism still needs in-depth study. |
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| Keywords: | layer chlorogenic acid E.coli O78 abdominal air sac infection immune function |
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