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| 不同饲喂水平对绵羊睾丸发育、睾酮合成相关基因及雄激素受体(AR)表达的影响 | |
| 引用本文： | 石磊,牛宏泽,姚晓磊,宋瑞高,吕丽华,张春香,张建新,任有蛇. 不同饲喂水平对绵羊睾丸发育、睾酮合成相关基因及雄激素受体(AR)表达的影响[J]. 畜牧兽医学报, 2021, 52(5): 1317-1327. DOI: 10.11843/j.issn.0366-6964.2021.05.017 |
| 作者姓名： | 石磊 牛宏泽 姚晓磊 宋瑞高 吕丽华 张春香 张建新 任有蛇 |
| 作者单位： | 山西农业大学动物科学学院, 太谷 030801 |
| 基金项目： | 山西省青年三晋学者专项（2016）；现代农业产业技术体系建设专项基金（CARS-38）；山西省基础研究计划（青年）项目（2012021027-6） |
| 摘 要： | 旨在研究不同饲喂水平对绵羊睾丸发育、睾酮(T)合成相关基因与雄激素受体(androgen receptor,AR)表达的影响.本研究采用单因素完全随机试验设计,将18只健康、体重相近((35±0.5)kg)的杜泊羊(♂)×晋中绵羊(♀)杂F1代4月龄公羔随机分为3组,每组6只,分别按照自由采食(AL组)、自由采食量的6... |
| 关 键 词： | 饲喂水平 绵羊 睾丸 睾酮 雄激素受体 |
| 收稿时间： | 2020-08-31 |
Effects of Different Feeding Levels on Testis Development and the Expression of Steroidogenesis-Related Genes and Androgen Receptor (AR) in Sheep |
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| SHI Lei,NIU Hongze,YAO Xiaolei,SONG Ruigao,Lü Lihua,ZHANG Chunxiang,ZHANG Jianxin,REN Youshe. Effects of Different Feeding Levels on Testis Development and the Expression of Steroidogenesis-Related Genes and Androgen Receptor (AR) in Sheep[J]. Chinese Journal of Animal and Veterinary Sciences, 2021, 52(5): 1317-1327. DOI: 10.11843/j.issn.0366-6964.2021.05.017 | |
| Authors: | SHI Lei NIU Hongze YAO Xiaolei SONG Ruigao Lü Lihua ZHANG Chunxiang ZHANG Jianxin REN Youshe |
| Affiliation: | College of Animal Science, Shanxi Agricultural University, Taigu 030801, China |
| Abstract: | The objective of this study was to investigate the effects of different feeding levels on testicular development, testosterone (T) concentration, the expression of testosterone synthesis-related genes and androgen receptor (AR) in testis of sheep. Eighteen healthy 4-month-old Dorper×Jinzhong crossed F1 ram lambs with similar body weight of ((35±0.5) kg) were selected and randomly divided into 3 groups for a comparative slaughter trial. They were offered one type of feed at 100%(AL group), 65%(AL65 group) and 40%(AL40 group) of ad libitum intake, respectively. When any lamb in AL group reached a body weight of 50 kg, all animals were slaughtered. After the testicular circumference and length were examined, tissue samples were collected for analyzing testosterone concentration and histological parameters using ELISA and H-E staining method, respectively. The expression of testosterone synthesis-related genes and AR mRNA were detected using quantitative real-time PCR. Localization and quantification of AR in testis of lambs were determined by immunohistochemistry and Western blotting analysis. The results showed that the length and circumference of testis in the AL40 group were significantly lower than those in the AL group(P<0.05), no significant difference was observed between the AL40 and AL65 groups(P>0.05). The thickness of germinal epithelium of lambs in AL40 group was not significantly different from that in AL65 group, but both were significantly lower than that in AL group (P<0.05). The density of spermatogenic cells and leydig cells were remarkably increased with the increasing feeding levels(P<0.05). Testosterone concentration, the expression of STAR, 3β-HSD and AR genes and AR protein were also significantly increased(P<0.05). Immunopositive product of AR was detected in leydig cells,primary spermatocytes, spermatids, peritubular myoid cells and perivascular smooth muscle cells. In conclusion, the testicular development, T level, the expression of testosterone synthesis-related genes and AR were regulated by dietary nutrition level. Dietary nutrition could modulate the process of spermatogenesis by regulating T concentration and the sensitivity of spermatogenic cells to T, which could influence the sexual maturation and reproductive performance of male animals. |
| Keywords: | feeding level sheep testis testosterone androgen receptor |
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