| 沼泽红假单胞菌与枯草芽孢杆菌混施对水稻根域细菌多样性与功能的影响 |
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| 引用本文： | 张娜,朱艳,肖娴,赵远. 沼泽红假单胞菌与枯草芽孢杆菌混施对水稻根域细菌多样性与功能的影响[J]. 植物营养与肥料学报, 2022, 28(1): 58-71. DOI: 10.11674/zwyf.2021262 |
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| 作者姓名： | 张娜 朱艳 肖娴 赵远 |
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| 作者单位： | 1.常州大学环境与安全工程学院，江苏常州 213000 |
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| 基金项目： | 国家自然科学基金(42007027);常州市科技支撑计划(社会发展)(CE20205002)。 |
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| 摘 要： | [目的]微生物菌肥作为一种绿色环保的生物肥料,可以改善土壤质量,促进作物生长.以水稻根际和根表微生物为对象,探究不同微生物菌肥单施和混施对其群落多样性和功能的影响.[方法]采用盆栽试验,设单施沼泽红假单胞菌(Rhodopseudomonas palustris)、单施枯草芽孢杆菌(Bacillus subtilis)及...
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| 关 键 词： | 微生物菌肥 水稻 根际细菌 根表细菌 |
| 收稿时间： | 2021-05-17 |
Effects of co-inoculation of Rhodopseudomonas palustris and Bacillus subtilis on the diversity and function of soil bacteria in rice root zone |
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| ZHANG Na,ZHU Yan,XIAO Xian,ZHAO Yuan. Effects of co-inoculation of Rhodopseudomonas palustris and Bacillus subtilis on the diversity and function of soil bacteria in rice root zone[J]. Plant Nutrition and Fertilizer Science, 2022, 28(1): 58-71. DOI: 10.11674/zwyf.2021262 |
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| Authors: | ZHANG Na ZHU Yan XIAO Xian ZHAO Yuan |
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| Affiliation: | 1.School of Environmental and Safety Engineering, Changzhou University, Changzhou, Jiangsu 213000, China |
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| Abstract: | 【Objectives】 Microbial fertilizer is effective to improve soil fertility and promote crop growth. We studied the effects of single- and co-inoculation of two microbial fertilizers on rice rhizosphere and rhizoplane bacterial diversity and function. 【Methods】 A rice pot experiment was conducted, and four treatments were set up, i.e. clean water as control, single inoculation of Rhodopseudomonas palustris (R. palustris), single inoculation of Bacillus subtilis (B. subtilis), and co-inoculation of both (Mix). At harvest of rice, soil samples were collected around rice plant of 10 cm × 10 cm× 10 cm, and rhizoplane and rhizosphere samples were separated from the soil samples, to determine bacterial diversity and function by 16S rDNA high-throughput sequencing and functional prediction. 【Results】 All the three biofertilizer treatments increased rice yield and the setting rate, of which Mix treatment increased the most by 13.7% and 17.2%, respectively. All the three biofertilizer treatments did not change soil physicochemical properties significantly at harvest stage of rice. Compared with control, the application of the two functional bacteria did not significantly affect the diversity and function of rhizosphere bacterial community, but single-inoculation of B. subtilis and co-inoculation reduced Chao1 richness and Shannon-Wiener index of rhizoplane bacterial community. An increase in the relative abundance of rhizoplane dominant phylum Proteobacteria (average increase of 3%, 10% and 13% by single-inoculation of R. palustris, single-inoculation of B. subtilis and co-inoculation of both, respectively) was found. Microbial fertilizer treatments caused variations in the dominant genera, such as increasing the relative abundances of Novosphingobium, Flavobacterium and Rhizobium and reducing those of Anaeromyxobacter, Bradyrhizobium, Pleomorphomonas, Nocardioides, Propionicimonas and Methanobacterium, changed rhizoplane bacterial community structure, and increased their functional gene abundance. The abundance of both rhizosphere and rhizoplane bacterial genes involved in metabolism, genetic information processing, environmental information processing, and cellular processes were positively correlated with panicle seed setting rate (r = 0.60–0.68, P< 0.05). The increase of functional gene abundance was related to the change of dominant genera, and such change in rhizoplane bacterial community had a greater impact on the bacterial functional gene abundance. 【Conclusion】 Application of R. palustris improved rice yields significantly, and its co-inoculation with B. subtilis was more effective than single-inoculation. Compared with control, both co-inoculation and single-inoculation changed the rhizoplane bacterial diversity and increased the abundance of dominant bacteria and functional genes. The effect of co-inoculation on bacterial community was similar to single-inoculation of B. subtilis, but different with that of single-inoculation of R. palustris. Microbial fertilizer can indirectly promote crop growth by affecting the diversity of root zone bacterial communities. |
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| Keywords: | microbial fertilizer rice rhizosphere bacteria rhizoplane bacteria |
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