| 褪黑素对镉致鸭大脑皮质毒性损伤的保护作用 |
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| 引用本文： | 张文华,闻双全,王莉,徐明畅,马勇刚,邹辉,顾建红,刘学忠,卞建春,刘宗平,袁燕. 褪黑素对镉致鸭大脑皮质毒性损伤的保护作用[J]. 畜牧兽医学报, 2021, 52(10): 2953-2959. DOI: 10.11843/j.issn.0366-6964.2021.010.026 |
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| 作者姓名： | 张文华 闻双全 王莉 徐明畅 马勇刚 邹辉 顾建红 刘学忠 卞建春 刘宗平 袁燕 |
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| 作者单位： | 1. 扬州大学兽医学院, 扬州 225009;2. 江苏高校动物重要疫病与人畜共患病防控协同创新中心, 扬州 225009 |
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| 基金项目： | 国家自然科学基金（31772808）；江苏高校优势学科建设工程资助项目（PAPD）；扬州大学优秀青年骨干教师资助；扬州大学校教改课题（YZUJX2019—51C） |
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| 摘 要： | 为探究褪黑素对镉致鸭大脑皮质毒性损伤的保护作用，本试验将16只20日龄高邮鸭随机分为4组，分别为对照组、褪黑素组、镉组、镉与褪黑素共处理组。对照组鸭自由采食饮水；褪黑素组鸭自由饮用含有0.2 mg·L-1褪黑素的水；镉组鸭自由采食拌有2 mg·kg-1氯化镉的饲料；镉与褪黑素共处理组鸭自由饮用含有0.2 mg·L-1褪黑素水的同时自由采食拌有2 mg·kg-1氯化镉的饲料。60 d后，剖检并采集鸭大脑皮质。比色法检测大脑皮质中丙二醛（MDA）和总抗氧化能力（T-AOC）的水平，ELISA法检测肿瘤坏死因子（TNF-α）、白介素-1β（IL-1β）的含量，免疫组化染色观察Nrf2核转位，免疫印迹法检测Nrf2、HO-1的蛋白表达。结果显示，与对照组相比，镉组鸭大脑皮质发生明显Nrf2核转位，T-AOC水平极显著降低（P<0.01），MDA、TNF-α、IL-1β含量和Nrf2、HO-1蛋白表达量极显著升高（P<0.01）；与镉组相比，镉与褪黑素共处理组鸭大脑皮质Nrf2核转位减少，T-AOC水平显著升高（P<0.05），MDA、TNF-α、IL-1β含量和Nrf2、HO-1蛋白表达量显著或极显著降低（P<0.05或P<0.01）。综上，褪黑素对镉所致的鸭大脑皮质毒性损伤具有一定的保护作用。
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| 关 键 词： | 褪黑素 镉 鸭 大脑皮质 毒性损伤 |
| 收稿时间： | 2021-03-26 |
Protective Effect of Melatonin on Toxic Damage Caused by Cadmium in Cerebral Cortex of Duck |
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| ZHANG Wenhua,WEN Shuangquan,WANG Li,XU Mingchang,MA Yonggang,ZOU Hui,GU Jianhong,LIU Xuezhong,BIAN Jianchun,LIU Zongping,YUAN Yan. Protective Effect of Melatonin on Toxic Damage Caused by Cadmium in Cerebral Cortex of Duck[J]. Chinese Journal of Animal and Veterinary Sciences, 2021, 52(10): 2953-2959. DOI: 10.11843/j.issn.0366-6964.2021.010.026 |
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| Authors: | ZHANG Wenhua WEN Shuangquan WANG Li XU Mingchang MA Yonggang ZOU Hui GU Jianhong LIU Xuezhong BIAN Jianchun LIU Zongping YUAN Yan |
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| Affiliation: | 1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China |
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| Abstract: | In order to investigate the protective effect of melatonin on toxic damage caused by cadmium in cerebral cortex of duck, sixteen GaoYou ducks were randomly allocated into 4 groups:control group, melatonin-treated group, cadmium-treated group, cadmium and melatonin co-treated group. The ducks in the control group were free to eat and drink. The ducks in the melatonin group received drinking water containing 0.2 mg·L-1 melatonin. The ducks in the cadmium group received feeding mixed with 2 mg·kg-1 cadmium chloride. The ducks in the cadmium and melatonin co-treated group received drinking water containing 0.2 mg·L-1 melatonin plus feeding mixed with 2 mg·kg-1 cadmium chloride. After 60 d, the cerebral cortices of ducks were dissected and collected. The levels of malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in cerebral cortex of duck were measured by colorimetry, the contents of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were detected by ELISA, the nuclear translocation of Nrf2 was observed by immunohistochemical staining, the protein expression of Nrf2 and HO-1 was detected by Western blot. The results showed that compared with the control group, the Nrf2 nuclear translocation was obvious, the level of T-AOC showed extremely significant decrease (P<0.01), the contents of MDA, TNF-α, IL-1β, the protein expression of Nrf2 and HO-1 showed extremely significant increase (P<0.01) in the cerebral cortex of duck in cadmium-treated group. Compared with the cadmium-treated group, the Nrf2 nuclear translocation was decreased, the level of T-AOC showed significant increase (P<0.05), the contents of MDA, TNF-α, and IL-1β, the protein expression of Nrf2 and HO-1 showed significant or extremely significant decrease (P<0.05 or P<0.01) in the cerebral cortex of duck in cadmium and melatonin co-treated group. The results indicate that melatonin has a protective effect on toxic damage caused by cadmium in cerebral cortex of duck. |
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| Keywords: | melatonin cadmium duck cerebral cortex toxic damage |
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