| 高效液相色谱法分离茶儿茶素氧化聚合物 |
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| 引用本文： | 李春美,谢笔钧. 高效液相色谱法分离茶儿茶素氧化聚合物[J]. 茶叶科学, 2001, 21(1): 69-71,68. DOI: 10.13305/j.cnki.jts.2001.01.017 |
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| 作者姓名： | 李春美 谢笔钧 |
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| 作者单位： | 华中农业大学食品科技系!湖北武汉430070,华中农业大学食品科技系!湖北武汉430070 |
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| 摘 要： | 采用H2 O2 氧化以EGCG为主的儿茶素 ,并将所得的氧化产物进行了MS分析和HPLC分离。结果表明所得氧化产物主要为儿茶素的二至五聚体 ;一般的等度洗脱和线性梯度洗脱不能使儿茶素氧化聚合物各组分分离 ,往流动相中添加微量EDTA或乙酸铵并采用线性梯度和阶段梯度相结合的洗脱模式既可使氧化聚合物同小分子的儿茶素分开 ,也可使氧化聚合物各组分基本分离。这为儿茶素氧化聚合物的制取及分离提供了一条新思路
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| 关 键 词： | 高效液相色谱 分离 儿茶素 聚合物 |
| 文章编号： | 1000-369X（2001）01-0069-03 |
Separation of Tea Catechin Oxy-polymers by High Performance Liquid Chromatography |
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| LI Chun mei,XIE Bi jun [WTBX]. Separation of Tea Catechin Oxy-polymers by High Performance Liquid Chromatography[J]. Journal of Tea Science, 2001, 21(1): 69-71,68. DOI: 10.13305/j.cnki.jts.2001.01.017 |
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| Authors: | LI Chun mei XIE Bi jun [WTBX] |
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| Affiliation: | LI Chun mei,XIE Bi jun [WT6BX] |
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| Abstract: | Tea catechin oxy polymers were obtained by oxidizing tea catechin with H 2O 2 and their isolation conditions of HPLC were studied MS showed that their molecular weight range from 906 4 to 1962 4, which suggested that they might be dimer to pentamer of EGCG; Isocratic and linear gradient elution could not separate them from each other, but a method of combining linear and step gradients elution could separate them from each other This HPLC method might provide a new way to separate oxy polymers of catechin |
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| Keywords: | HPLC Separation Tea catechin Oxy polymers |
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