| 百合新品种引种筛选及组培快繁技术研究 |
| |
| 引用本文： | 李茂娟,廖祯妮,邓少华,王华龙,曹罕升. 百合新品种引种筛选及组培快繁技术研究[J]. 中国农学通报, 2019, 35(22): 65-70. DOI: 10.11924/j.issn.1000-6850.casb19030095 |
| |
| 作者姓名： | 李茂娟 廖祯妮 邓少华 王华龙 曹罕升 |
| |
| 作者单位： | 郴州市林业科学研究所 |
| |
| 基金项目： | 2016年湖南省林业科技计划项目“百合优良品种的引种选育与栽培应用”(XLK201655)。 |
| |
| 摘 要： | 为了开展优良百合品种资源的引种收集与合理利用，推广百合作为林下花卉栽植，本研究以8个百合品种(‘Caesars Palace’、‘Black Out’、‘Donato’、‘Eyeliner’、‘Manissa’、‘Ceb Dazzle’、‘Brindisi’、‘Bonsoir’)为引种供试材料，建立评分表，对其在郴州地区生长的主要观赏性状及适应性进行观测评比，并对评分最高的百合品种开展组培快繁体系建立研究。研究表明，采用5分制评分法综合筛选出3个适宜郴州地区栽培的观赏百合品种（‘Caesars Palace’综合得分4.78、‘BlackOut’综合得分4.33、‘Donato’综合得分4.67）。将得分最高的‘Caesars Palace’百合品种的鳞茎为外植体开展组培研究，结果表明，以中层和内层的鳞片诱导效果较好；最佳增殖培养基为MS+ 6-BA 1.0 mg/L+ NAA 0.2 mg/L，增殖系数达3.13；最佳生根培养基为1/2MS+ NAA 0.5 mg/L；移栽基质配方为黄心土:泥炭土:珍珠岩=5:4:1，成活率可达85%以上。
|
| 关 键 词： | 树脂 树脂 武当道茶多糖 脱色率 脱蛋白率 多糖保留率 |
| 收稿时间： | 2019-03-22 |
Introduction and Tissue Culture Propagation Technology of New Lily Cultivars |
| |
| Abstract: | The paper aims to collect and utilize the resources of new Lily varieties, and to promote and popularize the cultivated area of Lily varieties as a kind of under-forest flower, In this study, 8 cultivars of Lilium cultivars (‘Caesars Palace’, ‘Black Out’, ‘Donato’, ‘Eyeliner’, ‘Manissa’, ‘Ceb Dazzle’, ‘Brindisi’ and ‘Bonsoir’) were introduced and planted in Chenzhou, and the ornamental characteristics and adaptability were observed and evaluated by a score sheet. Meanwhile, experiments were carried out for the rapid propagation of Lilium by tissue culture technology, which based on the highest scores. Firstly, the results showed that ‘Caesars Palace’, ‘Black Out’, ‘Donato’ were suitable for planting in Chenzhou, and their scores were 4.78, 4.33 and 4.67, respectively. Secondly, the experiments of tissue culture, using the bulb of ‘Caesars Palace’ as explants, were indicated that the medium and inner of bulb scales would promote induction of shoots easier. Thirdly, the optimal medium for plantlets proliferation was MS medium containing 1.0 mg/L 6-BA and 0.2 mg/L NAA, and for root induction and elongation, 1/2MS medium plus NAA at the concentration of 0.5 mg/L showed good effect. Additionally, the survival rate of transplantation was about 85% under the optimal transplantation matrix of V (yellow soil): V(peat soil): V(perlite)= 5:4:1. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
|
点击此处可从《中国农学通报》下载全文 |
|
