| 高致病性猪繁殖与呼吸综合征的调查报告 |
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| 引用本文： | 栾智华,周洪臣. 高致病性猪繁殖与呼吸综合征的调查报告[J]. 中国畜牧兽医, 2012, 39(6): 242-244 |
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| 作者姓名： | 栾智华 周洪臣 |
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| 作者单位： | 辽宁省盘锦市动物疫病预防控制中心,辽宁盘锦 124010 |
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| 摘 要： | 为了解2011年盘锦地区免疫猪群中高致病性猪繁殖与呼吸综合征的免疫效果和感染状况,采用ELISA和荧光定量RT-PCR方法分别检测了本年度1-12月采集到的2160份猪血清和120份猪组织样品。结果表明,受检的猪组织样品中均无高致病性猪繁殖与呼吸综合征抗原;大洼县、盘山县、兴隆台区、双台子区猪繁殖与呼吸综合征抗体阳性率分别为82.2%、81.1%、78.8%、81.1%;种猪场、商品代猪场、散养户的猪繁殖与呼吸综合征抗体阳性率分别为90%、81.3%、75%。从总体来看,2011年盘锦地区的猪繁殖与呼吸综合征免疫状况良好,无猪繁殖与呼吸综合征疫情发生。
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| 关 键 词： | 高致病性猪繁殖与呼吸综合征 酶联免疫吸附试验 荧光RT-PCR |
Investigation on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome |
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| LUAN Zhi-hua , ZHOU Hong-chen. Investigation on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(6): 242-244 |
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| Authors: | LUAN Zhi-hua ZHOU Hong-chen |
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| Affiliation: | Panjin Center for Prevention and Control of Animal Epidemic Disease, Panjin 124010,China |
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| Abstract: | To investigate the infection status and the immune effect of the highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) from immunized pig populations of Panjin city in 2011, 2160 serum samples and 120 tissue specimens were collected from pig farms in Panjin city from January to December in 2011, and examined by enzyme linked immunosorbent assay (ELISA) and quantitative real-time PCR respectively. The results showed that there were not the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-RRSV) in the tissues, the antibodies positive rate in Dawa,Panshan, Xinglongtai,Shuangtaizi was 82.2%, 81.1%,78.8%,81.1%, respectively; the antibodies positive rate in swine breeding farm,commercial pig farm,peasant household pigs was 90%,81.3%,75%, respectively. In summary, the immune status of HP-PRRS was fine, HP-PRRS did not break out in this year. |
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| Keywords: | HP-PRRS ELISA quantitative real-time PCR |
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