MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle |
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| Authors: | Hong Quyen Dang XU Gu-li HOU Lian-jie XU Jian HONG Guang-liang Chingyuan Hu WANG Chong |
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| Affiliation: | 1. National Engineering Research Center for Breeding Swine Industry/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China;2. Bac Giang Agricultural and Forestry University, Bac Giang 26000, Viet Nam;3. Department of Human Nutrition, Food and Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Honolulu, HI 96822, USA |
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| Abstract: | Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. Micro RNAs(mi RNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. mi R-22 differentially expresses in embryonic and adult skeletal muscle. However, the underlying mechanism is unclear. In this study, we investigated mi R-22 function in proliferation and differentiation of porcine satellite cells(PSCs) in skeletal muscle. Our data show that mi R-22 expressed in both proliferation and differentiated PSCs and is significantly upregulated(P0.05) during differentiation. After treated with the mi R-22 inhibitor, PSCs proliferation was significantly increased(P0.05), as indicated by the up-regulation(P0.01) of cyclin D1(CCND1), cyclin B1(CCNB1) and down-regulation(P0.05) of P21. Conversely, over-expression of mi R-22 resulted in opposite results. Differentiation of PSCs was significantly suppressed(P0.05), evidenced by two major myogenic markers: myogenin(Myo G) and myosin heavy chain(My HC), after transfecting the PSCs with mi R-22 inhibitor. Opposite results were demonstrated in the other way around by transfection with mi R-22 mimics. In conclusion, the data from this study indicated that mi R-22 inhibited the PSCs proliferation but promoted their differentiation. |
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| Keywords: | miR-22 skeletal muscle porcine satellite cells proliferation differentiation |
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