| 暹罗芽孢杆菌ZHX-10的分离鉴定及其对花生白绢病的生防效果 |
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| 引用本文： | 张霞,许曼琳,郭志青,于静,吴菊香,于建垒,刘同金,李新国,迟玉成,万书波. 暹罗芽孢杆菌ZHX-10的分离鉴定及其对花生白绢病的生防效果[J]. 中国油料作物学报, 2020, 42(4): 674. DOI: 10.19802/j.issn.1007-9084.2019207 |
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| 作者姓名： | 张霞 许曼琳 郭志青 于静 吴菊香 于建垒 刘同金 李新国 迟玉成 万书波 |
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| 作者单位： | 1. 山东省花生研究所，山东，青岛，266100；2. 山东省农业科学院植物保护研究所，山东济南，250100；3. 山东省农业科学院生物技术研究中心，山东济南，250100；4. 山东省农业科学院，山东济南，250100 |
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| 基金项目： | 国家重点研发计划项目（2017YFD0201600）；山东省自然科学基金（ZR2018LC015）；山东省农业科学院农业科技创新工程（CXGC2018E21；CXGC2016B11）；山东省重大科技创新工程项目（2019JZZY010702）；山东省现代农业产业技术体系花生创新团队建设项目（SDAIT-04-07） |
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| 摘 要： | 为筛选可用于防治花生白绢病的生防菌株，采用稀释涂布平板法，从土传病害发生严重的花生田中分离获得了1 株拮抗细菌ZHX-10，结合形态学观察、生理生化鉴定及16S rDNA、gyrB 序列分析对其进行了种类鉴定，并初步测定了ZHX-10的菌悬液、挥发性气体、发酵液对白绢病菌的抑制效果。结果表明：ZHX-10菌株为暹罗芽孢杆菌Bacillus siamensis，其菌悬液、挥发性气体和发酵液均能有效抑制白绢病菌菌丝的生长；同时离体叶片接菌实验发现ZHX-10菌悬液产生的挥发性气体能够有效降低白绢病的发病情况。研究结果表明，ZHX-10 在白绢病菌生物防治中具有较好的应用潜力和较高的研究价值。
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| 关 键 词： | 花生白绢病 分离鉴定 暹罗芽孢杆菌 抑制效果 生物防治 |
Isolation and identification of Bacillus sinensis ZHX-10 and analysison its biological control activities against Sclerotium rolfsii |
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| ZHANG Xia,XU Man-lin,GUO Zhi-qing,YU Jing,WU Ju-xiang,YU Jian-lei,LIU Tong-jin,LI Xin-guo,CHI Yu-cheng,WAN Shu-bo. Isolation and identification of Bacillus sinensis ZHX-10 and analysison its biological control activities against Sclerotium rolfsii[J]. Chinese Journal of Oil Crop Sciences, 2020, 42(4): 674. DOI: 10.19802/j.issn.1007-9084.2019207 |
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| Authors: | ZHANG Xia XU Man-lin GUO Zhi-qing YU Jing WU Ju-xiang YU Jian-lei LIU Tong-jin LI Xin-guo CHI Yu-cheng WAN Shu-bo |
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| Affiliation: | 1. Shandong Peanut Research Institute, Qingdao 266100, China;2. Institute of Plant Protection, Shandong Acade⁃my of Agricultural Sciences, Jinan 250100, China;3. Biotechnology Research Center of Shandong Academy of Agri⁃cultural Sciences, Jinan 250100, China;4. Shandong Academy of Agricultural Sciences, Jinan 250100, China |
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| Abstract: | In order to screen biocontrol strains for southern stem rot, a bacteria ZHX-10 was isolated from pea⁃nut field with serious soil-borne diseases by dilution plate method. The strain ZHX-10 was identified based on mor⁃phological characteristics, physiological, biochemical characteristics, 16S rDNA and gyrB sequence analysis. Theinhibitory effects of bacterial suspension, volatile gas, fermentation broth from strain ZHX-10 against Sclerotiumrolfsii Sacc. were preliminarily determined. The results showed that ZHX-10 was Bacillus siamensis, and its suspen⁃sion, volatile gas and fermentation broth could effectively inhibit the growth of Sclerotium rolfsii Sacc.. At the sametime, it was found that the volatile gas produced by ZHX-10 suspension could effectively reduce the incidence ofSclerotium rolfsii Sacc. on peanut leaves. It indicated that strain ZHX-10 had high research value and potentials forbiological control of Sclerotium rolfsii Sacc. |
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| Keywords: | Sclerotium rolfsii Sacc. isolation and identification Bacillus siamensis inhibitory effects biological control |
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