| 橡胶树两种叶部病害拮抗木霉T008的筛选、鉴定及室内防效评价 |
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| 引用本文： | 薛鸣,战鑫,王睿,邢梦玉,刘铜,侯巨梅. 橡胶树两种叶部病害拮抗木霉T008的筛选、鉴定及室内防效评价[J]. 中国生物防治学报, 2022, 38(1): 125-132. DOI: 10.16409/j.cnki.2095-039x.2021.06.017 |
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| 作者姓名： | 薛鸣 战鑫 王睿 邢梦玉 刘铜 侯巨梅 |
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| 作者单位： | 1. 海南大学热带农林生物灾害教育部重点实验室, 海口 570228;2. 海南省农用生物制剂创制工程研究中心, 海口 570228 |
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| 基金项目： | 海南省自然科学基金（319MS015）；国家自然科学基金（32060589） |
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| 摘 要： | 采用平板稀释法从海南橡胶园16份根际土壤中分离获得6株拮抗木霉,通过对峙培养、非挥发性和挥发性物质及几丁质酶活测定,从中筛选出一株对橡胶树胶孢炭疽菌Colletotrichum gloeosporioides和多主棒孢菌Corynespora cassiicola均有较好抑制作用的菌株T008;采用分子生物学鉴定方法,...
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| 关 键 词： | 木霉菌 橡胶病害 几丁质酶 形态学鉴定 分子鉴定 |
| 收稿时间： | 2021-03-30 |
Screening and Identification of Antagonistic Trichoderma T008 for two Leaf Diseases of Rubber Tree and Evaluation of Indoor Control Efficacy |
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| XUE Ming,ZHAN Xin,WANG Rui,XING Mengyu,LIU Tong,HOU Jumei. Screening and Identification of Antagonistic Trichoderma T008 for two Leaf Diseases of Rubber Tree and Evaluation of Indoor Control Efficacy[J]. Chinese Journal of Biological Control, 2022, 38(1): 125-132. DOI: 10.16409/j.cnki.2095-039x.2021.06.017 |
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| Authors: | XUE Ming ZHAN Xin WANG Rui XING Mengyu LIU Tong HOU Jumei |
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| Affiliation: | 1. Key Laboratory of Green Prevention and Control of Tropical Plant Disease and Pests(Hainan University), Ministry of Education, Haikou 570228, China;2. Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan, Haikou 570228, China |
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| Abstract: | Six Trichoderma strains were isolated from 16 rhizosphere soil samples of Hainan Rubber Plantation by the plate dilution method. An isolates T008 was screened out by dual culture, determination of non-volatile substance and volatile substances and chitinase activity, which had a significant inhibitory effect on Colletotrichum gloeosporioides and Corynespora cassiicola. The isolates T008 was identified as Trichoderma asperellum by molecular identification and morphological identification methods. The inhibition rates of Trichoderma asperellum T008 against C. gloeosporioides and C. cassiicola were 61.02% and 58.60%, respectively, which had a good preventive effect on these two diseases. These results indicated that the T. asperellum T008 could be used as a potential biocontrol agent against anthracnose and corynespora leaf fall disease of Hevea brasiliensis. |
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| Keywords: | Trichoderma rubber diseases chitinase morphological identification molecular identification |
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