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| 生防细菌A178的分子鉴定及抑菌机理的研究 | |
| 引用本文： | 陈莉. 生防细菌A178的分子鉴定及抑菌机理的研究[J]. 中国农学通报, 2009, 25(22): 241-244 |
| 作者姓名： | 陈莉 |
| 作者单位： | 1. 新疆农业职业技术学院,新疆昌吉,831100 2. 南京农业大学,南京,210095 3. 新疆农业科学院植保所,乌鲁木齐,833000 |
| 基金项目： | 国家科技部项目资助(2004BA901A36)国家公益行业(农业)项目，新疆维吾尔自治区科技厅项目资助 |
| 摘 要： | 通过培养形态、生理生化特征以及16SrDNA的分子序列同源性分析,16SrDNA测序结果在GeneBank数据库进行同源性比较,确定A178菌株为枯草芽胞杆菌（Bacillus subtilis）;采用平板对峙培养法,发现A178对棉花立枯病菌、棉花枯萎病菌和棉花黄萎病菌具有较强的抑菌作用,通过显微镜观察,发现拮抗细菌A178的主要拮抗机理是通过产生拮抗物质造成病原真菌菌丝体断裂、扭曲、畸形,抑制分生孢子的萌发,造成孢子畸形。 |
| 关 键 词： | 生防细菌 分子鉴定 抑菌机理 |
| 收稿时间： | 2009-06-09 |
| 修稿时间： | 2009-07-20 |
Molectial Identification and Mechanism of Antagonistic Bacteria A178 |
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| Chen Li,Mi Nawaer,Gong qin,Miao Weiguo,Nuerzi Ya,Liu Haiyang. Molectial Identification and Mechanism of Antagonistic Bacteria A178[J]. Chinese Agricultural Science Bulletin, 2009, 25(22): 241-244 | |
| Authors: | Chen Li Mi Nawaer Gong qin Miao Weiguo Nuerzi Ya Liu Haiyang |
| Affiliation: | Chen Li, Mi Nawaer, Gong qin, Miao Weiguo, Nuerzi Ya, Liu Haiyang (1Xinjiang agriculture vocation technical college, Xinjian Changji 831100; 2Nanjing Agricultural University, Nanjing 210095;＇Istitute of plant protection, Xinjiang academy of agricultural sciences, Urumqi 833000) |
| Abstract: | Established the position of A178 strains based on the results of morphologic characteristics, physiological and biochemical properties and phylogenetic analysis of 16SrDNA sequence. Eventually A178 was identified as Bacillus subtilis. And A178 showed stronger antagonism against Rhizoctonia solani, Fusarium oxysporumf, sp. vasinfectum and Verticillium dahliaeon by plate duel culture. And the examination of antagonistic mechanism under light microscope showed that antagonistic substance of A178 appeared to inhibit pathogens by leading to mycelium rupture, distortion, abnormality, and inhibition bourgeon of conidiophore, and leading to abnormality of conidiophore. |
| Keywords: | biological bacatiria| molecteul identification| Antagonistic mechanism |
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