| 一株抗马铃薯坏疽病生防细菌B-401产抑菌物质条件优化及稳定性 |
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| 引用本文： | 畅涛,冯中红,杨成德,薛莉. 一株抗马铃薯坏疽病生防细菌B-401产抑菌物质条件优化及稳定性[J]. 中国生物防治学报, 2016, 32(3): 365-371. DOI: 10.16409/j.cnki.2095-039x.2016.03.012 |
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| 作者姓名： | 畅涛 冯中红 杨成德 薛莉 |
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| 作者单位： | 甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心, 兰州 730070 |
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| 基金项目： | 国家自然科学基金(31160122) |
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| 摘 要： | 以马铃薯坏疽病菌为靶标菌株,采用含药平板法对高寒草地禾草内生细菌B-401产抑菌物质条件及抑菌物质的稳定性进行了研究。结果表明,生防菌株B-401产抑菌物质的最佳培养基配方为牛肉膏8g、酵母浸膏5.0g、葡萄糖10g、水1000mL,最适温度为23.4℃,最适pH值为7,最佳装液量为35.2mL/150mL三角瓶,最佳培养方式为光照条件下振荡培养24h,最适发酵时间为96h,优化验证试验结果表明,其对马铃薯坏疽病菌的EC50＝0.111μL/mL,是优化前EC50＝3.196μL/mL的3.47%。菌株B-401所产抑菌物质在25~90℃具有较好稳定性,抑菌活性均大于75%,在100℃及以上高温处理后抑菌物质活性降低;对酸碱性和紫外照射稳定,相对活性分别在77.45%和98%以上;对金属离子Na+、Mg2+和Mn2+稳定,相对活性达99%以上,而对Ag+、Cu2+、Zn2+和Fe3+较不稳定。
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| 关 键 词： | 马铃薯坏疽病菌 禾草内生菌 抑菌物质 培养条件 稳定性 |
| 收稿时间： | 2015-10-26 |
Optimizing the Culture Conditions and Research on Stabilities of Antagonistic Substance Produced by Antagonistic Bacteria B-401 aganist Phoma foveata |
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| CHANG Tao,FENG Zhonghong,YANG Chengde,XUE Li. Optimizing the Culture Conditions and Research on Stabilities of Antagonistic Substance Produced by Antagonistic Bacteria B-401 aganist Phoma foveata[J]. Chinese Journal of Biological Control, 2016, 32(3): 365-371. DOI: 10.16409/j.cnki.2095-039x.2016.03.012 |
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| Authors: | CHANG Tao FENG Zhonghong YANG Chengde XUE Li |
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| Affiliation: | College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem Gansu Agricultural University, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S. Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China |
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| Abstract: | UsingPhoma foveata as target pathogen, we studied the optimum culture conditions and stabilities of inhibitive substances secreted by antagonistic endophytic bacteria B-401 from alpine meadow pasture. The results showed that the optimal medium of antagonistic substances produced by B-401 was D broth (beef extract 8 g, yeast extract 5.0 g, glucose 10 g, water 1000 mL), the optimum culture conditions were temperature 23.4 ℃, pH 7, loading volume 35.2 mL/150 mL flask, the way of cultivation was shaking culture under 24 h illumination, the optimum fermentation time was 96 h. The antifungal substances secreted by B-401 were stable at 25―90 ℃, and the antifungal activities were all surpassed over 75%, and it was reduced after treatment at 100 ℃ or more. Also the relative activities were resistant to the metal ions Na+, Mg2+ and Mn2+, and become less stable to Ag+, Cu2+, Zn2+, Fe3+. |
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| Keywords: | Phoma foveata endophytes of grasses inhibitive substance culture conditions stability |
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