Phenotypic,molecular and pathological characterization of motile aeromonads isolated from diseased fishes cultured in Uruguay |
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| Authors: | Alejandro Perretta Karina Antúnez Pablo Zunino |
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| Affiliation: | 1. Facultad de Veterinaria, Instituto de Investigaciones Pesqueras, Universidad de la República, Montevideo, Uruguay;2. Department of Microbiology, Instituto de Investigaciones Biológicas “Clemente Estable”, Ministerio de Educación y Cultura, Montevideo, Uruguay |
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| Abstract: | Information about motile aeromonads from aquaculture systems of the Neotropical region is scarce. The aim of this study was to characterize motile Aeromonas isolated from ornamental and consumable fishes cultured in Uruguay. Biochemical and molecular methods were used for species identification. Antimicrobial susceptibility and the presence of virulence genes were evaluated. Genetic diversity was analysed by rep‐PCR, and virulence of the most representative isolates was determined by calculating the fifty lethal dose in experimentally challenged fish (Australoheros facetus). Aeromonas hydrophila and A. veronii were the most prevalent identified species (38.2% and 32.4%, respectively), whereas A. allosacharophila, A. bestiarium, A. caviae and A. punctata were less prevalent. This study constitutes the first report of these last four species in Uruguay. All isolates were resistant to at least three antimicrobials, and 82.3% of them showed multidrug resistance. Virulence genotypes were correlated with the Aeromonas species and haemolytic activity. The genotype act+/alt+/ast+/ela+/lip+ was the most prevalent (26.5%). A correlation between virulence genotypes and Aeromonas species was found. A. punctata showed a clonal structure according to rep‐PCR analysis, whereas other species showed high genetic diversity. The number of virulence genes of the isolates was related with virulence according to the experimental challenge assays. |
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| Keywords: | antimicrobial resistance experimental challenge molecular epidemiology motile aeromonads virulence |
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