| pH与盐度胁迫对鼠尾藻光合作用及抗氧化系统的影响 |
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| 引用本文： | 佘婷婷, 钟晨辉, 林琪, 唐隆晨, 宦忠艳, 周文发. 不同盐度低渗胁迫处理对龙须菜切段再生能力及光合生理的影响[J]. 水产学报, 2023, 47(12): 129604. DOI: 10.11964/jfc.20211013121 |
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| 作者姓名： | 佘婷婷 钟晨辉 林琪 唐隆晨 宦忠艳 周文发 |
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| 作者单位： | 1.福建省水产研究所，福建省海洋生物增养殖与高值化利用重点实验室，福建 厦门 361013;2.上海海洋大学水产与生命学院，上海 201306;3.莆田市正洋水产发展有限公司，福建 莆田 351100 |
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| 基金项目： | 国家现代农业产业技术体系专项(CARS-50)；福建省海洋与渔业结构调整专项(2021HYJG03)；福建省科技计划(2019NJJ009) |
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| 摘 要： | 为探究龙须菜对低渗胁迫的生理性适应，分析了龙须菜主枝切段在不同盐度低渗培养液(盐度19.500、13.000、6.500、0.000)下耐受不同胁迫时间(1、3、6、12和20 h)后，继续恢复正常盐度 (盐度 26.000)培养过程中的形态发生、光合生理指标和细胞器亚显微结构变化。结果显示，短于12 h的低渗 (盐度 0~19.500)胁迫可以促进龙须菜切段的出芽，其总出芽数均高于对照组 (盐度 26.000)。3 h的淡水胁迫后恢复至正常盐度培养，藻段再生过程的出芽数目较多，鲜重增加最多，表现出了明显的生长优势。龙须菜藻段经淡水胁迫3 h后恢复至正常盐度培养28 d，藻段的相对生长率(RGR)为0.91 %/d，较对照组RGR提高了61.27%。1和3 h淡水胁迫对龙须菜藻段的光合生理指标无明显的负面效应，且恢复至正常盐度培养后藻段的光合生理活性增强，提示低渗胁迫可能增强了藻段的细胞代谢活力。透射电镜观察表明，经淡水胁迫3 h后，表皮细胞内的红藻淀粉颗粒、质体小球及脂质体等为藻体生长发育提供能量的物质和质体再生所需中性脂原料明显增加，适应于藻段再生过程的新生芽形成。相反，长时间的淡水胁迫对色素体和类囊体等亚细胞器结构造成了不可逆的损伤。研究表明，龙须菜主枝切段经淡水3 h的浸泡有助于其再生过程的出芽，这将为龙须菜无性系苗种快速扩繁提供技术参考。本研究可为建立龙须菜无性系苗种快速扩繁技术提供参考数据。
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| 关 键 词： | 龙须菜 低渗胁迫 切段再生 光合生理 超微结构 |
| 收稿时间： | 2021-10-20 |
| 修稿时间： | 2021-11-28 |
Salinity tolerance of eukaryotic marine algae |
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| SHE Tingting, ZHONG Chenhui, LIN Qi, TANG Longchen, HUAN Zhongyan, ZHOU Wenfa. Effects of different hypo-osmotic stress treatments on budding regeneration and photosynthetic physiology of algal fragments in Gracilariopsis lemaneiformis[J]. Journal of fisheries of china, 2023, 47(12): 129604. DOI: 10.11964/jfc.20211013121 |
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| Authors: | SHE Tingting ZHONG Chenhui LIN Qi TANG Longchen HUAN Zhongyan ZHOU Wenfa |
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| Affiliation: | 1.Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China;2.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;3.Putian Zhengyang Fisheries Development Co., Ltd., Putian 351100, China |
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| Abstract: | In order to explore the physiological acclimation of Gracilariopsis lemaneiformis to hypo-osmotic stress, in this study, the morphogenesis, photosynthetic physiological responses and ultrastructure changes of algal fragments from the main branches after being cultivated at combinations of four different low salinities (S 19.500, S 13.000, S 6.500 and S 0.000) and for five different periods (1, 3, 6, 12 and 20 h) and then cultured at normal salinity for 28 days were analysed. The results showed that less than 12 h hypo-osmotic stress treatment could promote the regeneration of buds in algal fragments of G. lemaneiformis. After 3 hours of hypo-osmotic stress with freshwater, increased fresh weight and number of regenerated buds obviously emerged in these fragments during normal salinity of culture. The relative growth rate (RGR) of algal fragments treated with freshwater for 3 hours was 0.91%/d, which was 61.27% higher than that of the control group. Furthermore, 1 h or 3 h hypo-osmotic stress with freshwater had no significant negative effects on the photosynthetic physiological responses of algal fragments, manifesting their photosynthetic activity was stimulated after restoration to seawater at normal salinity. It suggested that the cell metabolic activity of algal fragments might be enhanced after hypo-osmotic stress with freshwater. Ultrastructural observations showed that abundant floridean starch grains and plastoglobuli, sporadic lipid droplets emerged in the epidermal cell after 3 hours of hypo-osmotic stress with freshwater which would provide metabolic energy and membrane components for the algal fragments upon budding regeneration. On the contrary, long-term hypo-osmotic stress with freshwater caused irreversible structural damages to the organelles such as chloroplasts and thylakoids. These results indicated that the 3-hour treatment of hypo-osmotic stress with freshwater for algal fragments from main branches would stimulate their budding regeneration. This study would provide technical reference for rapid asexual propagations of G. lemaneiformis seedlings. |
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| Keywords: | Gracilariopsis lemaneiformis hypo-osmotic stress fragment regeneration photosynthetic physiology ultrastructure |
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