|
|||||
|
|
| 土壤结构性质和磷脂剖面的联合保存: 三种干燥技术的比较 | |
| 引用本文： | L. J. DEACON,D. V. GRINEV,J. W. CRAWFORD,J. HARRIS,K. RITZ,I. M. YOUNG. 土壤结构性质和磷脂剖面的联合保存: 三种干燥技术的比较[J]. 土壤圈, 2008, 18(3): 284-287. DOI: 10.1016/S1002-0160(08)60018-1 |
| 作者姓名： | L. J. DEACON D. V. GRINEV J. W. CRAWFORD J. HARRIS K. RITZ I. M. YOUNG |
| 作者单位： | [1]National Soil Resources Institute, Department of Natural Resources, School of Applied Sciences, Cranfield University,Cranfield, MK430AL （UK） [2]SIMBIOS Centre, University of Abertay, Dundee, DD11HG （UK） [3]Integrated Earth Systems Science Institute, Department of Natural Resources, School of Applied Sciences, CranfieldUniversity, Cranfield, MK430AL （UK） |
| 摘 要： | There is a need to simultaneously preserve evidence of interactions between the biological community and soil structural properties of a soil in as near an intact （natural） state as possible. Three dehydration techniques were implemented and assessed for their ability to minimise disruption of both biological and physical properties of the same arable soil sample. Dehydration techniques applied until samples were at constant weight were i） air-drying at 20℃ （AD）; ii） -80 ℃ freeze for 24 h, followed by freeze-drying （-80FD）; and iii） liquid nitrogen snap freeze, followed by freeze-drying （LNPD） and were compared to a moist control. Physical structure was determined and quantified in three dimensions using X-ray computed tomography and microbial phenotypic community composition was assessed using phospholipid fatty acid （PLPA） profiling. This study confirms that any form of dehydration, when preparing soil for simultaneous biological and physical analysis, will alter the soil physical properties, and cause some change in apparent community structure. Freeze-drying （both the LNFD and -80FD treatments） was found to minimise disruption （when compared to the moist control soil） to both the soil physical properties and the community structure and is a preferable technique to air-drying which markedly alters the size and character of the pore network, as well as the phenotypic profile. The LNFD was the preferred treatment over the -80FD treatment as samples show low variability between replicates and a fast turn-around time between samples. Therefore snap freezing in liquid nitrogen, followed by freeze drying is the most appropriate form of dehydration when two sets of data, both physical and biological, need to be preserved simultaneously from a soil core. |
| 关 键 词： | 生物群落 脱水作用 磷脂 干燥环境 |
| 收稿时间： | 2008-01-22 |
| 修稿时间： | 20 March 2008. |
Simultaneous preservation of soil structural properties and phospholipid profiles: A comparison of three drying techniques |
|
| L. J. DEACON,D. V. GRINEV,J. W. CRAWFORD,J. HARRIS,K. RITZ and I. M. YOUNG. Simultaneous preservation of soil structural properties and phospholipid profiles: A comparison of three drying techniques[J]. Pedosphere, 2008, 18(3): 284-287. DOI: 10.1016/S1002-0160(08)60018-1 | |
| Authors: | L. J. DEACON D. V. GRINEV J. W. CRAWFORD J. HARRIS K. RITZ I. M. YOUNG |
| Affiliation: | aNational Soil Resources Institute, Department of Natural Resources, School of Applied Sciences, Cranfleld University, Cranfleld, MK43 0AL (UK) bSIMBIOS Centre, University of Abertay, Dundee, DD1 1HG (UK) cIntegrated Earth Systems Science Institute, Department of Natural Resources, School of Applied Sciences, Cranfleld University, Cranfleld, MK43 0AL (UK) |
| Abstract: | There is a need to simultaneously preserve evidence of interactions between the biological community and soil structural properties of a soil in as near an intact (natural) state as possible. Three dehydration techniques were implemented and assessed for their ability to minimise disruption of both biological and physical properties of the same arable soil sample. Dehydration techniques applied until samples were at constant weight were i) air-drying at 20 °C (AD); ii) −80 °C freeze for 24 h, followed by freeze-drying (−80FD); and iii) liquid nitrogen snap freeze, followed by freeze-drying (LNFD) and were compared to a moist control. Physical structure was determined and quantifled in three dimensions using X-ray computed tomography and microbial phenotypic community composition was assessed using phospholipid fatty acid (PLFA) proflling. This study conflrms that any form of dehydration, when preparing soil for simultaneous biological and physical analysis, will alter the soil physical properties, and cause some change in apparent community structure. Freeze-drying (both the LNFD and −80FD treatments) was found to minimise disruption (when compared to the moist control soil) to both the soil physical properties and the community structure and is a preferable technique to air-drying which markedly alters the size and character of the pore network, as well as the phenotypic proflle. The LNFD was the preferred treatment over the −80FD treatment as samples show low variability between replicates and a fast turn-around time between samples. Therefore snap freezing in liquid nitrogen, followed by freeze drying is the most appropriate form of dehydration when two sets of data, both physical and biological, need to be preserved simultaneously from a soil core. |
| Keywords: | biological community dehydration phospholipid proflle physical properties preservation |
| 本文献已被 维普 ScienceDirect 等数据库收录！ | |
| 点击此处可从《土壤圈》浏览原始摘要信息 | |
| 点击此处可从《土壤圈》下载全文 | |