Comparison of Milk Fat Globule Membrane Proteins in Milk Samples of Dairy Cow and Goat |
| |
| Authors: | ZHAO Xiao-wei YANG Yong-xin HUANG Dong-wei CHENG Guang-long ZHAO Hui-ling |
| |
| Affiliation: | Institute of Animal Sciences and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China |
| |
| Abstract: | In order to compare the difference of milk fat globule membrane（MFGM）proteins between dairy cow and goat milk,30 milk samples were collected in a dairy farm and a goat farm from Anhui area,respectively.Extracted proteins from the MFGM-enriched fractions were identified and quantified by LC/MS approach.The results showed that 284 and 334 proteins of MFGM from dairy cow and goat were identified,the biological processes of MFGM proteins were similar between dairy cow and goat which were mainly related to biological regulation,localization,transport,signal transduction and response to stimulus.Meanwhile,there were some differences in molecular functions,and protein binding and nucleotide binding were the most prevalent molecular functions in dairy cow MFGM proteins,while protein binding and structural molecule activity were the most prevalent molecular functions in goat MFGM proteins.And structural molecule activity was the main molecular functions among the difference proteins.Pathway analysis revealed that tight junction,axon guidance,antigen processing and presentation,complement and coagulation cascades were enrichment in dairy cow MFGM proteins,and regulation of actin cytoskeleton was enrichment in goat MFGM proteins.Those results revealed the protein expression pattern difference between MFGM protein of dairy cow and goat milk,and provide reference data for further exploring the molecular mechanism of synthetic milk fat globule. |
| |
| Keywords: | milk fat globule membrane LC/MS dairy cow milk goat milk |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
|
点击此处可从《中国畜牧兽医》下载全文 |
|
