Isolation and Identification of Local Aleutian Mink Virus Virulent Strain ADV-LN |
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| Authors: | JIA Yun LI Yan-wu SUN Ming-ying ZHANG Xue ZHANG Yan-yan SONG Da-he LIU Zhao WANG Quan-kai SUN Ying-jie |
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| Affiliation: | 1. Jilin Agricultural University, Changchun 130000, China;2. Liaoning Entry Exit Inspection and Quarantine Bureau, Dalian 116000, China;3. Liaoning Fisheries Technology Extension Station, Shenyang 116015, China;4. Sichuan Entry Exit Inspection and Quarantine Bureau, Chengdu 610000, China |
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| Abstract: | Mink suspected infection aleutian mink disease virus (ADV) from mink breeding areas in Liaoning province were tested with CIEP method.The mink with antibody to ADV were selected and culled.Liver,spleen,kidney and mesenteric lymph node samples were taken for pathological examination and the viruses were observed under electron microscope.The grinded tissue fluid filter was added penicillin and treptomycin and inoculated into CRFK cells and passaged by 6 times for virus isolation.And cells cultures were identified as ADV by PCR.Then they were inoculated into healthy mink.Three days later,the mink showed clinical signs,which including the loss of appetite,anemia,hair dull,antifeedant and binge drinking.Some minks showed neurological symptoms,manifested symptoms of convulsions,cramps,staggering gait,ataxia,or hind limb paralysis and died.The virus strains isolated and identified were named as the ADV-LN. |
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| Keywords: | aleutian mink disease virus isolation identification |
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