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| 果胶酶对莲心中黄酮类物质提取效果研究 | |
| 引用本文： | 肖贵平. 果胶酶对莲心中黄酮类物质提取效果研究[J]. 中国农学通报, 2012, 28(3): 267-271. DOI: 10.11924/j.issn.1000-6850.2011-2946 |
| 作者姓名： | 肖贵平 |
| 作者单位： | 福建农林大学食品科学学院,福州,350002 |
| 基金项目： | 福建省科技重大专项专题项目“建宁县特色农产品深加工技术创新示范”（2007SZ0001-9）; 福建省教育厅科技项目“莲心黄酮类物质提取工艺的研究与应用”（JB09110） |
| 摘 要： | 为了有效提高莲心中黄酮类物质的提取效果，采用果胶酶辅助提取方法，研究酶解温度、pH值、酶用量及酶解时间对莲心黄酮类物质提取效果的影响，通过L9(34)正交试验设计优选其最佳工艺条件。结果表明，影响莲心黄酮类物质提取效果的主次因素为酶用量>酶解温度>酶解时间>pH值，最佳提取工艺为酶解温度50℃，pH值4.5，酶用量0.6 mg/mL，酶解时间2.0 h，在此条件下莲心黄酮类物质提取率为1.271%。该酶法提取工艺，可以在较低的温度下提高提取效果，与传统的水提工艺(90℃、45 min)相比，莲心中黄酮类物质提取率提高了28.64%。 |
| 关 键 词： | 肥料利用率 肥料利用率 |
| 收稿时间： | 2011-10-12 |
| 修稿时间： | 2011-10-24 |
Study on Pectinase Assisted Extraction of Flavonoids from Plumula Nelumbinis |
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| Xiao Guiping. Study on Pectinase Assisted Extraction of Flavonoids from Plumula Nelumbinis[J]. Chinese Agricultural Science Bulletin, 2012, 28(3): 267-271. DOI: 10.11924/j.issn.1000-6850.2011-2946 | |
| Authors: | Xiao Guiping |
| Affiliation: | Xiao Guiping (College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002) |
| Abstract: | Improving the extraction efficiency of flavonoids from plumula nelumbinis, pectinase assisted extraction has been adopted. The effects of enzymatic hydrolysis temperature, pH value, enzyme dosage and enzymatic hydrolysis time on extraction yield of flavonoids from plumula nelumbinis were investigated. The processing parameters were optimized by orthogonal array design. The results showed that the order of the primary and secondary factors for the yield of flavonoids was enzyme dosageenzymatic hydrolysis temperatureenzymatic hydrolysis timepH value. The optimal extraction conditions were enzymatic hydrolysis temperature 50℃, pH 4.5, enzyme dosage 0.6 mg/mL, and enzymatic hydrolysis time 2.0 h, the extraction yield of flavonoids from plumula nelumbinis was 1.271% on optimum technological conditions. Compared to traditional water extraction method （90℃ 、45 min）, the pectinase assisted extraction method has the advantages of improving yield with lower extraction temperature. The extraction yield of flavonoids from plumula nelumbinis has increased by 28.64%. |
| Keywords: | plumula nelumbinis pectinase enzymatic extraction flavonoids |
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