Determination of selected antibiotics in farmed fish species using LC‐MS/MS |
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| Authors: | Mustafa Yipel Cemil Kürekci İbrahim O Tekeli Murat Metli Fatih Sakin |
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| Affiliation: | 1. Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Mustafa Kemal University, Hatay, Turkey;2. Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Mustafa Kemal University, Hatay, Turkey;3. Republic of Turkey Ministry of Food, Agriculture and Livestock, Hatay Food Control Laboratory, Hatay, Turkey |
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| Abstract: | The aim of the study was to monitor the occurrence of 37 antibiotics from different classes including fluoroquinolone, tetracycline, macrolide, sulphonamide, penicillin, amphenicol and cephalosporin in three different farmed fish species (n = 75; rainbow trout, see bass, gilthead see bream) from five cities in Turkey. A method for LC‐MS/MS was optimized and validated to assess these antibiotics. As a result, 25 samples showed contamination with at least one antibiotic residue. The most detected antibiotics were norfloxacin (NOR) (18.7%), marbofloxacin (MAR) (12%), tetracycline (TC) (10.7), danofloxacin (DAN) (9.3%), oxytetracycline (OTC) (9.3%), tilmicosin (TLM) (8%) and enrofloxacin (ENR) (6.7%). A total of 17.3% of samples examined were found to be contaminated with residues exceeding the maximum residue limits (MRLs). The results indicate that antibiotics are frequently used in aquaculture production without the lack of knowledge. This study also revealed that the consumption of farmed fish could produce a public health concern as the level of residues for some antibiotics was much higher than MRLs. |
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| Keywords: | aquaculture fish antibiotic residue LC‐MS/MS |
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