| 3-溴丙酮酸对美国白蛾核型多角体病毒的增效作用 |
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| 引用本文： | 刘瑞霞,李恩杰,柏超,王青华,段立清. 3-溴丙酮酸对美国白蛾核型多角体病毒的增效作用[J]. 林业科学研究, 2022, 35(6): 101-107. DOI: 10.13275/j.cnki.lykxyj.2022.006.011 |
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| 作者姓名： | 刘瑞霞 李恩杰 柏超 王青华 段立清 |
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| 作者单位： | 中国林业科学研究院森林生态环境与自然保护研究所/国家林业和草原局森林保护学重点实验室,北京 100091;内蒙古农业大学林学院,内蒙古 呼和浩特 010019;中国林业科学研究院华北林业实验中心,北京 102300;北京市新海园林工程有限公司,北京 100018;中国林业科学研究院森林生态环境与自然保护研究所/国家林业和草原局森林保护学重点实验室,北京 100091;内蒙古农业大学林学院,内蒙古 呼和浩特 010019 |
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| 基金项目： | 中国林科院中央级公益性科研院所基本科研业务费专项基金;国家重点研发计划 |
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| 摘 要： | 目的 利用3-溴丙酮酸（3-BrPA）作为美国白蛾核型多角体病毒（HycuNPV）的生物增效剂,探讨3-BrPA对HycuNPV的毒力和对美国白蛾生长发育的影响,为进一步推进HycuNPV杀虫剂的应用提供新的增效剂。 方法 通过室内在HycuNPV中添加不同剂量3-BrPA,分析其对HycuNPV的增效作用以及对美国白蛾生长发育的影响。 结果 室内生物活性结果表明,3-BrPA对HycuNPV具有增效作用,不仅可以提高HycuNPV的感染能力,还能提高杀虫速度。当3-BrPA的添加剂量为5.0、10.0和15.0 mg·g−1时,HycuNPV作用于美国白蛾3龄幼虫的LC50都有不同程度地降低;并且对HycuNPV的增效倍数（SR）分别为825.14、164.72和0.47,共毒系数（CTC）分别为82 613.63、16 579.25和147.27。不同浓度的HycuNPV在不同剂量的3-BrPA作用下,其杀虫效果各不相同。添加不同剂量的3-BrPA,LT50缩短了3.280～9.724 d。根据SR、CTC、LT50及ST等指标评判,在浓度为9.0 × 105 OBs·mL−1的HycuNPV中添加5.0 mg·g−1的3-BrPA时,增效作用最优。此外,3-BrPA和HycuNPV联合作用还显著抑制了幼虫的生长发育,且存活幼虫的化蛹率和羽化率均低于单独使用病毒杀虫剂。 结论 3-BrPA对HycuNPV具有增效作用,有望作为理想的病毒杀虫剂的增效剂。
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| 关 键 词： | 美国白蛾核型多角体病毒 3-溴丙酮酸 美国白蛾 增效作用 |
| 收稿时间： | 2022-02-06 |
Synergistic Effect of 3-bromopyruvate Combined with Hyphantria cunea Nucleopolyhedrovirus Against Hyphantria cunea (Drury) |
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| LIU Rui-xia,LI En-jie,BAI Chao,WANG Qing-hua,DUAN Li-qing. Synergistic Effect of 3-bromopyruvate Combined with Hyphantria cunea Nucleopolyhedrovirus Against Hyphantria cunea (Drury)[J]. Forest Research, 2022, 35(6): 101-107. DOI: 10.13275/j.cnki.lykxyj.2022.006.011 |
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| Authors: | LIU Rui-xia LI En-jie BAI Chao WANG Qing-hua DUAN Li-qing |
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| Affiliation: | 1. Institute of Forest Ecology, Environment and Nature Conservation, Chinese Academy of Forestry/Key Laboratory of Forest Frotection, State Forestry and Grassland Administration, Beijing 100091, China;2. , Forestry College, Inner Mongolia Agricultural University, Huhhot 010019, Inner Mongolia, China;3. North China Forestry Experimental Center, Chinese Academy of Forestry, Beijing 102300, China;4. Xinhai Landscape Engineering Co., Ltd., Beijing 100018, China |
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| Abstract: | Objective In this paper, we used 3-bromopyruvate (3-BrPA) as a biological synergist of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) to investigate the effect of 3-BrPA on the virulence of HycuNPV and the growth and development of Hyphantria cunea, which will provide new synergist resources for the application of HycuNPV insecticides. Method The synergistic effect on HycuNPV was analyzed by adding different doses of 3-BrPA incorporating with HycuNPV in laboratory. Result The bioassay results showed that 3-BrPA had a synergistic effect on HycuNPV. It could not only improve infectivity of HycuNPV, but also increase the insecticidal speed. When the dosages of 3-BrPA with 5.0, 10.0 and 15.0 mg·g−1 were added to HycuNPV, the LC50 of HycuNPV in the 3rd instar larvae of H.cunea decreased. The synergistic ratio (SR) of HycuNPV were 825.14, 164.72 and 0.47, and the co-toxicity coefficients (CTC) were 82613.63, 16579.25 and 147.27, respectively. Different concentrations of HycuNPV had different insecticidal effects under the different doses of 3-BrPA. Added different doses of 3-BrPA, the LT50 of HycuNPV against the larvae were shorten by 3.280～9.724 d. According to SR, CTC, LT50 and ST, the optimum synergistic effect was observed: 5.0 mg·g−1 3-BrPA + 9.0 × 105 OBs·mL−1 HycuNPV. In addition, the combination of 3-BrPA and HycuNPV significantly inhibited the growth and development of larvae, and the pupation and emergence rate of surviving larvae were significantly lower than that of the virus insecticide alone. Conclusion 3-BrPA has a synergistic effect on HycuNPV, and is expected to be an ideal synergistic resource of viral insecticides. |
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| Keywords: | Hyphantria cunea nucleopolyhedrovirus 3-bromopyruvate Hyphantria cunea (Drury) Synergistic effect |
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