| 玉米根际细菌群落特征及生防菌筛选 |
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| 引用本文： | 刘泉成,张茜茜,田雪亮,刘文德. 玉米根际细菌群落特征及生防菌筛选[J]. 中国生物防治学报, 2018, 34(5): 771-778. DOI: 10.16409/j.cnki.2095-039x.2018.05.017 |
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| 作者姓名： | 刘泉成 张茜茜 田雪亮 刘文德 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;2 河南科技学院, 新乡 453003 |
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| 基金项目： | 植物病虫害生物学国家重点实验室开放基金（SKLQF201508）；河南省高等学校青年骨干教师项目（2016GGJS-217） |
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| 摘 要： | 为了明确玉米根际细菌群落结构特征并从中筛选玉米病害生防资源，本研究采用培养法从玉米根际分离得到1584株细菌，分别属于变形菌门、厚壁菌门、放线菌门和拟杆菌门，其中变形菌门为优势菌群。在属水平上，假单胞菌为优势菌群。以玉米大斑菌为靶标菌，通过平板对峙方法，筛选出162株具有抑菌效果的菌株，其中1株抑菌效果最强，根据形态学和分子生物学结合的方法，将其鉴定为解淀粉芽胞杆菌。该菌对4种玉米病害病原菌具有广谱的抑菌效果，且含有脂肽类抗生素合成基因，其脂肽类物质粗提液对病原菌的抑制率高达81.56%。总体来看，该菌株具有较大的生防潜力，可作为生防菌防治玉米病害。
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| 关 键 词： | 根际微生物 可培养细菌 解淀粉芽胞杆菌 生物防治 玉米 |
| 收稿时间： | 2018-03-15 |
Composition of Bacterial Community in Maize Rhizosphere and Screening of Biocontrol Bacteria Strains |
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| LIU Quancheng,ZHANG Xixi,TIAN Xueliang,LIU Wende. Composition of Bacterial Community in Maize Rhizosphere and Screening of Biocontrol Bacteria Strains[J]. Chinese Journal of Biological Control, 2018, 34(5): 771-778. DOI: 10.16409/j.cnki.2095-039x.2018.05.017 |
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| Authors: | LIU Quancheng ZHANG Xixi TIAN Xueliang LIU Wende |
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| Affiliation: | 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Henan Institute of Science and Technology, Xinxiang 453003, China |
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| Abstract: | To clarify composition bacterial community in maize rhizosphere and screen biocontrol resources for maize diseases, a total of 1584 bacteria strains were isolated from maize rhizosphere by culture-dependent method. The bacteria were classified into Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, among which Proteobacteria were the dominant groups. At genus level, Pseudomonas was the most abundant groups. A total of 162 bacteria strains with antagonism against Setosphearia turcica were selected through plate confrontation method and strain D3 had the strongest antifungal effect. Moreover, strain D3 was identified as Bacillus amyloliquefaciens by morphological and molecular methods. Strain D3 had broad-spectrum antifungal effect on maize fungal pathogens, including S. turcica, Colletotrichum graminicola, Fusarium avenaceum and Curvularia lunata. Using the specific primers of lipopeptide gene, the related genes could be amplified by PCR in strain D3. Additionally, cell-free filtrate and the crude extract of lipopeptide from strain D3 had biocontrol effect on pathogen with the inhibition rate of 81.56%. In conclusion, strain D3 can be used as a new biocontrol microorganism against maize fungal diseases. |
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| Keywords: | rhizosphere microbes culturable bacteria Bacillus amyloliquefaciens biological control maize |
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