| 缺硼对脐橙幼苗硼分配及叶片细胞壁组分硼含量的影响 |
| |
| 引用本文： | 刘桂东,胡萍,张婧卉,周高峰,曾钰,钟八莲,姜存仓. 缺硼对脐橙幼苗硼分配及叶片细胞壁组分硼含量的影响[J]. 植物营养与肥料学报, 2018, 24(1): 179-186. DOI: 10.11674/zwyf.17107 |
| |
| 作者姓名： | 刘桂东 胡萍 张婧卉 周高峰 曾钰 钟八莲 姜存仓 |
| |
| 作者单位： | 1.赣南师范大学生命与环境科学学院/国家脐橙工程技术研究中心，江西赣州 341000 |
| |
| 基金项目： | 国家自然科学基金项目（31701871）；2011协同创新中心项目（2014QC15）资助。 |
| |
| 摘 要： | 【目的】硼在维持细胞壁正常结构方面具有重要的作用，前期结果证实缺硼严重的脐橙叶片细胞壁结构改变程度也更大，但这种变化与细胞壁组分中硼的含量变化是否有关尚不清楚。本研究通过分析缺硼对脐橙幼苗各部分硼分配及叶片细胞壁组分硼含量的影响，明确缺硼症状表现及细胞壁结构变化程度与细胞壁各组分中硼含量变化之间的关系。【方法】以纽荷尔脐橙幼苗为试材，利用营养液培养方法进行缺硼处理，测定根、砧木茎、接穗茎、上部叶、下部叶、叶片细胞壁以及细胞壁各组分硼含量的变化情况。【结果】缺硼处理9周后上部叶出现叶片卷曲及叶片失绿等症状，而下部叶没有出现任何可见的症状。缺硼处理的脐橙幼苗各部位硼含量和硼吸收量均显著降低，缺硼降低了硼向地上部的相对分配比例且上部叶受到的影响程度更大。在硼正常供应条件下，上部叶和下部叶游离态硼、原生质体硼和细胞壁硼的含量和相对分配比例没有显著差异，说明硼在不同类型脐橙叶片细胞各组分中的分配是相对稳定的。缺硼后水溶性硼 (包括游离态硼和原生质体硼) 在脐橙上部叶和下部叶中都降到极低的水平，尤其是原生质体硼百分含量下部叶甚至是低于上部叶的。缺硼后细胞壁硼占总硼的比例则由22%左右增加到80%以上。与叶片中硼含量的变化趋势一致，缺硼以后虽然上部叶和下部叶细胞壁硼含量都显著降低，但上部叶降低的程度远大于下部叶。进一步分析细胞壁组分硼含量变化，发现缺硼显著降低了上部叶细胞壁中离子结合态果胶硼含量而对下部叶的无明显影响，其他组分硼含量的变化趋势下部叶和上部叶一致。【结论】原生质体硼含量的高低并不是决定缺硼症状的主要因素，离子结合态果胶与硼的结合能力对缺硼条件下细胞壁的结构及缺硼症状表现起着至关重要的作用。
|
| 关 键 词： | 硼 脐橙 细胞壁 叶片黄化 |
| 收稿时间： | 2017-03-28 |
Effect of boron deficiency on boron distribution in different plant parts and boron concentration in leaf cell wall components in navel orange plants |
| |
| LIU Gui-dong,HU Ping,ZHANG Jing-hui,ZHOU Gao-feng,ZENG Yu,ZHONG Ba-lian,JIANG Cun-cang. Effect of boron deficiency on boron distribution in different plant parts and boron concentration in leaf cell wall components in navel orange plants[J]. Plant Nutrition and Fertilizer Science, 2018, 24(1): 179-186. DOI: 10.11674/zwyf.17107 |
| |
| Authors: | LIU Gui-dong HU Ping ZHANG Jing-hui ZHOU Gao-feng ZENG Yu ZHONG Ba-lian JIANG Cun-cang |
| |
| Affiliation: | 1.College of Life and Environmental Sciences/National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou, Jiangxi 341000, China |
| |
| Abstract: | 【Objectives】Boron (B) plays an important role in maintaining the normal structure of cell walls. The previous results showed that the cell wall structure of leaves with severe B deficiency had a lot of change, but it was not clear whether this change was related to the change of B concentration in the cell wall components. This paper aims to clarify the relationship between B deficiency symptoms and changes of cell wall structure and B content in cell wall component.【Methods】In this study, the changes of B distribution in different plant parts and B concentrations in leaf cell wall and cell wall components of navel orange plants were measured using hydroponics method.【Results】The symptoms of leaf chlorosis and curling of the leaves were observed only in the upper leaves of B-deficient plants. The lower leaves did not show any visible symptoms. The B concentration and B accumulation in different parts of the boron-deficient navel orange plants were significantly decreased. Boron deficiency reduced the relative distribution ratio of B in the shoots and the greater impact on the upper leaves. There was no evident difference in concentrations of free B, protoplast B and cell wall B in leaves between the two types of leaves under normal B condition. However, the water-soluble B (including free B and protoplast B) decreased to extremely low levels in the upper and lower leaves of navel orange under B deficiency, especially the protoplast B in the lower leaves was even lower than that in the upper leaves. In control plants, the upper and lower leaves contained only about 22% of cellular B in the cell wall, whereas in B-deficient plants the cell walls of the upper and lower leaves contained >80% of total cell B. Furthermore, the concentrations of B in lower leaves and their cell walls were much higher than those in the upper leaves and their cell walls under B-deficient conditions. Further analysis of the changes of B content in cell wall components showed that B deficiency significantly reduced the boron content in the ion-bound pectin in the upper leaf cell wall and had no significant effect on that in the lower leaf cell wall. In contrast, no significant difference was found in changes of B content in other wall components due to B deficiency between the upper leaves and lower leaves. In addition, the structural changes of the upper leaf cell wall were much larger than those of the lower leaf cell wall under B deficiency.【Conclusions】Boron concentration in the protoplast was not the main factor determining the symptoms of B deficiency, while the binding capacity of ion-bound pectin to B was crucial to the structure of cell wall and the occurrence of B deficiency symptoms in navel orange leaves. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《植物营养与肥料学报》浏览原始摘要信息 |
|
点击此处可从《植物营养与肥料学报》下载全文 |
|
