| 叶用芥菜小孢子离体培养条件 |
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| 引用本文： | 吴慧敏,何 勇,邵泱峰,朱祝军,符庆功. 叶用芥菜小孢子离体培养条件[J]. 浙江农林大学学报, 2014, 31(2): 315-321. DOI: 10.11833/j.issn.2095-0756.2014.02.023 |
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| 作者姓名： | 吴慧敏 何 勇 邵泱峰 朱祝军 符庆功 |
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| 作者单位： | 1.浙江农林大学 浙江省农产品品质改良技术研究重点实验室, 浙江 临安 3113002.浙江省临安市农业技术推广中心, 浙江 临安 311300 |
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| 基金项目： | 浙江省教育厅资助项目(2273000001) |
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| 摘 要： | 以5个叶用芥菜Brassica juncea var. foliosa品种为试材, 研究了游离小孢子培养过程中基因型、低温预处理、高温热激、更新及加液和秋水仙碱等对小孢子胚状体发生的影响。结果表明:有4个品种获得胚状体, 各品种间胚状体产量存在显著差异, ‘特选九心芥’胚产量最高;4℃低温预处理24~48 h及33~34℃高温热激处理24~48 h对诱导胚状体发生和发育有促进作用;更新和添加培养基处理也可促进胚状体发生, 以质量浓度为170.0 g·L-1的蔗糖起始培养48 h后添加等质量浓度90.0 g·L-1蔗糖培养基处理效果最佳;1~10 mg·L-1秋水仙碱24~48 h处理可提高小孢子培养反应, 增加供试材料胚产量。
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| 关 键 词： | 园艺学 叶用芥菜 小孢子培养 胚状体 热激 秋水仙碱 |
| 收稿时间： | 2013-03-21; |
In vitro microspore culture of leaf mustard |
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| WU Huimin,HE Yong,SHAO Yangfeng,ZHU Zhujun,FU Qinggong. In vitro microspore culture of leaf mustard[J]. Journal of Zhejiang A&F University, 2014, 31(2): 315-321. DOI: 10.11833/j.issn.2095-0756.2014.02.023 |
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| Authors: | WU Huimin HE Yong SHAO Yangfeng ZHU Zhujun FU Qinggong |
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| Affiliation: | 1.The Key of Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A & F University, Lin'an 311300, Zhejiang, China2.Agricultural Technology Promotion Center of Lin'an City, Lin'an 311300, Zhejiang, China |
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| Abstract: | In order to improve the isolated microspore culture system in Brassica juncea var. foliosa, five different genotypes of Brassica juncea var. foliosa were used to investigate the contribution of genotype, cool-pretreatment, heat shock, medium replacement and addition, as well as concentration of colchicine on microspore embryogenesis in vitro. Results showed that embryoids were obtained from four genotypes, and significant differences （P＜0.05, Duncan’s MRT） were observed for embryo yield with the highest yield being ‘Texuan-jiuxin’. For all cultivars, 24-48 h of cool-pretreatment at 4 ℃ and heat shock at 33-34 ℃ improved embryo yields. Also, medium addition and replacement enhanced microspore embryogenesis with the best treatment using a constant volume for a 90.0 g·L-1 sucrose medium supplemented to a 170.0 g·L-1 sucrose concentration medium after 48 h. In addition, treating the freshly isolated microspore with 1-10 mg·L-1 colchicine for 24-48 h greatly improved（P＜0.05, Duncan’s MRT）embryo yields of the four cultivars. The present results could be used to obtain haploid efficiently for the plant breeding and basic research in Brassica juncea var. foliosa. ［Ch， 1 fig. 5 tab. 19 ref.］ |
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| Keywords: | horticulture leaf mustard microspore culture embryoid heat shock colchicine |
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