| 辣椒热激蛋白HSP90家族基因鉴定及分析 |
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| 引用本文： | 王 静,谭放军,梁成亮,张西露,欧立军,Niran Juntawong,王 飞,焦春海,邹学校,陈文超. 辣椒热激蛋白HSP90家族基因鉴定及分析[J]. 园艺学报, 2020, 47(4): 665-674. DOI: 10.16420/j.issn.0513-353x.2019-0470 |
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| 作者姓名： | 王 静 谭放军 梁成亮 张西露 欧立军 Niran Juntawong 王 飞 焦春海 邹学校 陈文超 |
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| 作者单位： | 1湖南省蔬菜研究所，长沙410000；2湖北省农业科学院，武汉430064；3泰国农业大学，曼谷10900 |
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| 基金项目： | 国家重点研发计划项目（2016YFE0205500） |
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| 摘 要： | 借助生物信息学工具对辣椒热激蛋白HSP90家族基因进行鉴定,并分析其理化性质、结构特征、系统进化关系以及在各组织器官和高温胁迫下的表达模式。结果表明,辣椒基因组中含有7个HSP90家族基因,分布于5条染色体上,内含子数2～19不等,含有5个保守基序;系统进化关系分析显示辣椒7个HSP90家族基因分为3组;亚细胞定位结果显示辣椒HSP90蛋白定位于细胞质与内质网中;基于已发表的转录组数据进行组织表达分析,HSP90在不同组织中的表达模式不同;高温处理可不同程度地激活HSP90的表达。
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| 关 键 词： | 辣椒 HSP90家族基因 特征分析 表达分析 |
Genome-wide Identification and Analysis of HSP90 Gene Family in Pepper |
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| WANG Jing,TAN Fangjun,LIANG Chengliang,ZHANG Xilu,OU Lijun,Niran Juntawong,WANG Fei,JIAO Chunhai,ZOU Xuexiao,CHEN Wenchao. Genome-wide Identification and Analysis of HSP90 Gene Family in Pepper[J]. Acta Horticulturae Sinica, 2020, 47(4): 665-674. DOI: 10.16420/j.issn.0513-353x.2019-0470 |
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| Authors: | WANG Jing TAN Fangjun LIANG Chengliang ZHANG Xilu OU Lijun Niran Juntawong WANG Fei JIAO Chunhai ZOU Xuexiao CHEN Wenchao |
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| Affiliation: | 1.Hunan Vegetable Research Institute，Changsha 410000，China；2Hubei Academy of Agricultural Sciences，Wuhan 430064，China；3Kasetsart University，Bangkok 10900，Thailand |
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| Abstract: | The pepper HSP90 gene family was identified and analyzed. Their physicochemical properties，structural characteristics，phylogenetic relationships，tissue-specific expression patterns and heat stress responses were also analyzed. The results showed that 7 genes of HSP90 were identified and they were located on five chromosomes. The HSP90 gene family contained 2–19 introns and 5 conserved motifs. The HSP90 genes were divided into three groups. Subcellular localization analysis indicated that HSP90 targeted the cytoplasm and endoplasmic reticulum. Moreover，the HSP90 gene family showed different expression profile in different tissues by analyzing published RNA-Seq data. And the expressions of HSP90 were induced by heat stress to varying degrees. |
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| Keywords: | pepper HSP90 gene family feature analysis expression analysis |
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