| 香蕉枯萎病拮抗菌贝莱斯芽胞杆菌的筛选鉴定及其生物学特性 |
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| 引用本文： | 杨迪,杜婵娟,张晋,潘连富,叶云峰,黄思良,付岗. 香蕉枯萎病拮抗菌贝莱斯芽胞杆菌的筛选鉴定及其生物学特性[J]. 中国生物防治学报, 2021, 37(1): 165-171. DOI: 10.16409/j.cnki.2095-039x.2021.01.031 |
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| 作者姓名： | 杨迪 杜婵娟 张晋 潘连富 叶云峰 黄思良 付岗 |
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| 作者单位： | 1. 南阳师范学院生命科学与农业工程学院, 南阳 473061;2. 广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室, 南宁 530007;3. 广西农业科学院园艺研究所, 南宁 530007 |
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| 基金项目： | 广西自然科学基金(2017GXNSFDA198002);广西创新驱动发展专项(桂科AA18118028-5);广西农业科学院科技发展基金(桂农科2018YT15,31960520,2020YM96);广西作物病虫害生物学重点实验室基金(17-259-47-ST-1)。 |
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| 摘 要： | 香蕉枯萎病是目前香蕉产业面临的毁灭性病害,目前尚无特别有效的防治药剂.为发掘香蕉枯萎病的生防菌资源,本研究通过初筛和复筛获得两株对香蕉枯萎病菌具有较强拮抗活性的菌株Blz67和Blz02.依据形态学观察及生理生化测试结果,并结合16S rRNA和gyrA基因序列分析,将两株拮抗菌鉴定为贝莱斯芽胞杆菌Bacillus v...
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| 关 键 词： | 香蕉枯萎病 贝莱斯芽胞杆菌 生物防治 |
| 收稿时间： | 2020-05-28 |
Screening,Identification and Biological Characteristics of Bacillus velezensis with Antagonst Activity against Banana Fusarium Wilt |
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| YANG Di,DU Chanjuan,ZHANG Jin,PAN Lianfu,YE Yunfeng,HUANG Siliang,FU Gang. Screening,Identification and Biological Characteristics of Bacillus velezensis with Antagonst Activity against Banana Fusarium Wilt[J]. Chinese Journal of Biological Control, 2021, 37(1): 165-171. DOI: 10.16409/j.cnki.2095-039x.2021.01.031 |
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| Authors: | YANG Di DU Chanjuan ZHANG Jin PAN Lianfu YE Yunfeng HUANG Siliang FU Gang |
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| Affiliation: | 1. School of Lifescience and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;2. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Institute of Plant Protection Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;3. Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning 530007, China |
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| Abstract: | Banana Fusarium wilt caused by Fusarium oxysporum f.sp.cubense(Foc)is a destructive disease in global banana industry.Till now,there is still no means available for effective control of the disease in field.In order to explore the biocontrol agents against banana Fusarium wilt,two antagonistic bacterial strains Blz67 and Blz02 were screened out by primary and secondary selections.The two strains were both identified as Bacillus velezensis by morphological observations,physiological and biochemical tests,combined with 16S rRNA and gyrA gene sequences analysis.The results of biological characteristics tests showed that the optimum temperature for growth of both strains were 31℃,and the optimum pH value both were 5.5.The control efficacies of the strains Blz02 and Blz67 against banana Fusarium wilt in green house were 63.33%and 40.00%under greenhouse conditions,respectively,indicating that both strains had a promising prospect in biological control of banana Fusarium wilt. |
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| Keywords: | banana Fusarium wilt Bacillus velezensis biological control |
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