Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius) semen |
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| Authors: | Mariana Santos Florbela Soares Márcio Moreira José Beirão |
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| Affiliation: | 1. Faculdade de Medicina Veterinária, Pólo Universitário do Alto da Ajuda, Lisbon, Portugal;2. IPMA ‐ Instituto Português do Mar e da Atmosfera, EPPO ‐ Esta??o Piloto de Piscicultura de Olh?o, Olh?o, Portugal;3. Faculty of Biosciences and Aquaculture, Nord University, Bod?, Norway |
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| Abstract: | Meagre (Argyrosomus regius) is considered a potential candidate for aquaculture diversification in southern Europe. The main objective of this experiment was to develop a cold storage protocol for meagre semen to facilitate artificial reproduction techniques. Three extenders (non‐activating medium, 0.9% NaCl, and 0.9% NaCl with glycine and glucose) in three different sperm:extender dilutions (1:4, 1:9 and 1:19) were tested in a full factorial design. The quality parameters assessed along the storage time were the sperm motility, viable sperm percentage, adenosine triphosphate (ATP) content, and bacterial growth. The 0.9% NaCl and 0.9% NaCl with glycine and glucose extenders and the 1:4 and 1:9 dilutions maintained a higher sperm motility and a higher sperm linearity for a longer period time. Sperm viability was maintained at a higher value over a longer period with the 0.9% NaCl and 0.9% NaCl with glycine and glucose extenders. Sperm motility and viability appeared to be the main parameters showing the loss of semen quality during cold storage. Meagre semen demonstrated an ability to be stored for up to 10 days at 4°C when using 0.9% NaCl in a 1:4 dilution. These results contribute to a better understanding of the causes of fish semen quality deterioration during cold storage. |
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| Keywords: | refrigeration reproduction sciaenidae sperm |
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